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Citations for Thermo Fisher :
251 - 300 of 10000+ citations for IL 4 Human CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... sections were fixed in ∼100ul of 4% paraformaldehyde (PFA; Thermo Fisher Scientific, Rockford, IL, USA) at room temperature for 15 minutes and then washed 2 times for 5 minutes each ...
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bioRxiv - Neuroscience 2019Quote: ... loaded onto a 4-12% NuPage Bis-Tris gels (Thermo Fisher Scientific, Rockford, IL, USA). The proteins were revealed using Pierce Silver Staining kit (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: Ectocervical biopsies were fixed for 48 hours in 4% formaldehyde (Thermo Fisher Scientific, Rockford, IL) and paraffin-embedded to generate 5 μm sections used for hematoxylin and eosin (H&E ...
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bioRxiv - Microbiology 2021Quote: ... Goat anti-mouse and anti-human antibodies pre-coupled to Alexa Fluor 647 (Invitrogen, Rockford, IL, USA) were used as secondary antibodies in flow cytometry experiments ...
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bioRxiv - Cancer Biology 2021Quote: ... Equal amounts of conditioned media were analyzed by ELISA (IL-1 beta Human ELISA Kit, Thermofisher KHC0011), using manufacturer protocol ...
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bioRxiv - Immunology 2023Quote: Sandwich enzyme-linked immunosorbent assay detection (ELISA) kit for human IL-1β (Invitrogen, catalog # 88-7261-88) was used at the specified temperature and conditions according to the manufacturer’s instructions.
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bioRxiv - Biophysics 2023Quote: ... human IL-2 (Cat# PHC0026) and CellTrace™ CFSE (Cat# C34570) were purchased from ThermoFisher (Waltham, MA). Bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2020Quote: ... CHO and Fao cells were transfected with DNA using Lipofectamine reagent (Invitrogen) or polyethylenimine (PEI-MAX ...
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bioRxiv - Microbiology 2021Quote: ... HEK/CHO cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) alongside 10% fetal bovine serum (FBS ...
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bioRxiv - Synthetic Biology 2020Quote: ... CHO-K1 were maintained in Minimum Essential Medium α (α-MEM, Gibco). All media were supplemented with 10% FBS (Atlanta BIO) ...
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bioRxiv - Biochemistry 2020Quote: ... Bioreactors were fed CHO CD EfficientFeed A (A1023401, Invitrogen, called bioreactor H1) or EfficientFeed B (A1024001 ...
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bioRxiv - Microbiology 2020Quote: ... in CHO-S-SFM media with hypoxanthine and thymidine (Life Technologies, # 12052098) using polyethylenimine (PEI ...
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bioRxiv - Biochemistry 2022Quote: ... CHO-K1 cells were cultured in F12 Nutrient Mixture (Ham) media (Gibco) with L-Glutamine ...
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bioRxiv - Bioengineering 2022Quote: ... The cells were maintained in CD-CHO medium (Life Technologies, Paisley, UK) at 36.5 °C ± 0.5 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... Chinese hamster ovary (CHO-K1) cells were cultured in F-12 (Gibco) containing 10% FBS ...
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bioRxiv - Microbiology 2021Quote: ... CHO cells were treated with 100 μL of either Opti-MEM (Gibco) or PI-PLC from Bacillus cereus (Invitrogen ...
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bioRxiv - Bioengineering 2021Quote: ... cultures were transfected using the Expifectamine CHO Transfection Kit (Thermo Fisher Scientific), following manufacturer instructions for the Standard Protocol ...
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bioRxiv - Molecular Biology 2020Quote: CHO-K1 cells were maintained in adherent culture in DMEM: F12 (Gibco) supplemented with 5% Fetal Bovine Serum (Gibco) ...
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SPNS1 is required for the transport of lysosphingolipids and lysoglycerophospholipids from lysosomesbioRxiv - Biochemistry 2022Quote: HEK293 cells and CHO cells were cultured in DMEM (Gibco, Cat 11995065) media supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Biophysics 2023Quote: CHO cells (ATCC) were grown in complete DMEM/F12 medium (ThermoFisher, #11320033) containing 10% fetal bovine serum (ThermoFisher ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... С6 and CHO/5-HT2C were maintained in the F12 medium (Invitrogen). In all cases ...
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bioRxiv - Immunology 2021Quote: ... containing the cytokines IL-4 (500 U/mL) and GM-CSF (800 U/mL) (both Gibco) for differentiation into DCs ...
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bioRxiv - Immunology 2020Quote: ... were used for T cell activation and expansion in the condition of human recombinant IL-2 (Gibco, PHC0021) or where indicated ...
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bioRxiv - Immunology 2022Quote: ... Human IFNg and IL-13 cytokine secretion was measured by ELISA using eBioscience Ready-SET-Go! (now ThermoFisher) ELISA kits according to the manufacturer’s protocol.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Pro-inflammatory cytokine (IL-8) and (IL-6) release was determined using the IL-6 and IL-8 ELISA kit (Invitrogen) according to the manufacturer’s instructions (ThermoFisher).
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bioRxiv - Immunology 2020Quote: ... and Luminex (Cytokine Human Magnetic 30 Plex Panel, Thermo Scientific, n=4) per manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... IL-5 and IL-13 (Invitrogen, Massachusetts, USA) were all measured according to kit protocols ...
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bioRxiv - Developmental Biology 2021Quote: Chinese hamster ovary (CHO) cells were cultured in HAMs F-12 (Gibco BRL) containing 10% Foetal serum ...
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bioRxiv - Synthetic Biology 2020Quote: ... HEK293T and CHO-K1 cells were transfected with Lipofectamine® 3000 (ThermoFisher Scientific) according to manufacturer’s instructions and 300 ng total DNA (500 ng in Fig ...
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bioRxiv - Bioengineering 2019Quote: ... cells were adapted from CD-CHO medium to FortiCHO (Gibco, Carlsbad, CA, USA), OptiCHO (Gibco) ...
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bioRxiv - Bioengineering 2019Quote: ... This cell line was derived from CHO-S cells (Gibco, Catalog No. A11364). In order to find the best combination of basal media and feed that could result in optimum cell growth ...
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bioRxiv - Biochemistry 2019Quote: ... which secrete large amounts of the NPC2 protein into CD CHO media (Invitrogen), using a 10 kDa cutoff flow filtration membrane (Millipore ...
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bioRxiv - Biochemistry 2020Quote: ... The fresh media and media supplements tested included CD CHO medium (10743029, ThermoFisher), CHO CD EfficientFeed A (A1023401 ...
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bioRxiv - Biochemistry 2020Quote: ... CHO cells expressing P-cadherin were cultured in Ham’s 12 medium (Life Technologies) containing 10% fetal bovine serum (FBS) ...
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bioRxiv - Neuroscience 2020Quote: CHO (Freestyle) cells were grown in serum-free Freestyle media (Gibco, 12651-014) and transfected with human cDNA expression plasmids with N-terminal Myc tags obtained from Sinobiological ...
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bioRxiv - Neuroscience 2022Quote: ... CHO cells were loaded with FM4-64FX dye (15 μM, Thermo Fisher Scientific) applied for 15 min at 37°C and 5% CO2 in 4 mM K+ buffer ...
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bioRxiv - Biochemistry 2022Quote: The resulting pApoA1-ApoM plasmid was transfected into adherent CHO-S cells (InvitroGen) using PEI (Polyethylenimine ...
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bioRxiv - Cell Biology 2020Quote: ... CHO cells were detached using Hyclone® HyQTase (Thermo Fisher Scientific Inc, #SV300.30.01), and resuspended in warm ...
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bioRxiv - Microbiology 2020Quote: ... CHO-HVEM cells were propagated in Ham’s F12 nutrient mixture (Gibco/Life Technologies) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2020Quote: ... CHO-HVEM cells were propagated in Ham’s F12 nutrient mixture (Gibco/Life Technologies) supplemented with 10% FBS ...
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bioRxiv - Immunology 2021Quote: The prefusion stabilized HexaPro spike protein27 with and without a 3xGGGS spacer and mCRAMP sequence added to the C-terminus were produced by Celonic AG (Basel, Switserland) by transient expression in Expi-CHO cell cultures (ThermoFisher Scientific) as previously described33 ...
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bioRxiv - Genetics 2019Quote: ... and CHO cell lines were cultured in 1x MEM (Gibco, Cat#11430-030) supplemented with non-essential amino acids (Gibco ...
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bioRxiv - Synthetic Biology 2022Quote: ... HEK293T and CHO-K1 were analysed with the Attune NxT flow cytometer (ThermoFisher). For each sample 10000 viable cells were recorded ...
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bioRxiv - Neuroscience 2024Quote: ... Kv2.1-CHO cells were detached in a PBS-EDTA solution (15040-066, Gibco), spun at 500 g for 2 minutes and then resuspended in 50% cell culture media and 50% CHO-external recording solution ...
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bioRxiv - Cell Biology 2023Quote: Interleukin-6 levels in supernatants were determined using commercially available human IL-6 ELISA kits according to the manufacturer’s protocol (Invitrogen). A detailed description of the procedure are reported in Supporting methods ...
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bioRxiv - Physiology 2021Quote: ... The total protein was inoculated with anti-HA magnetic beads at 4 C (Thermo Scientific, Rockford, IL) overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... Heat denatured protein samples were separated using a 4–12% NuPAGE gel (Thermo Scientific, Rockford, IL, USA). Proteins were transferred to PVDF membranes at 30 volts for 90 minutes ...
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bioRxiv - Immunology 2023Quote: ... IL-4 and IFN-ᵧ in supernatant was measured according to the manufacturer’s protocol (all Life Technologies).
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bioRxiv - Cell Biology 2021Quote: LX-2 and TWNT-4 human HSCs were lysed in TRIzol (Life Technologies) and total RNA was purified following the supplied protocol ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed 4 × in TBST and 30 μl of anti-human (Invitrogen) horseradish peroxidase-conjugated antibodies diluted 1:5,000 was added to each well and incubated at 37°C ...