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Citations for Thermo Fisher :
451 - 500 of 7935 citations for IL 12RB1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: HEK293 cells were grown and maintained in DMEM/F12 medium (Gibco/Thermo Fisher Scientific ...
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bioRxiv - Genomics 2021Quote: HEK293 cells were cultured in DMEM (GIBCO; Cat. No. 10313-039) supplemented with 10% FBS and 1% penicillin/streptomycin ...
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bioRxiv - Cancer Biology 2022Quote: ... HEK293 cells were maintained in Dulbecco’s Modified Eagle’s medium (DMEM) (Gibco) supplemented with 10% (v/v ...
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bioRxiv - Immunology 2022Quote: ... HEK293-F cells were grown in suspension using FreeStyle medium (Invitrogen). All cell lines were tested and confirmed mycoplasma-free using the protocol for Myco-Alert- Mycoplasma Detection Kit (LT07-218 ...
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bioRxiv - Cell Biology 2022Quote: ... and FLP-In-293 (HEK293-FLP) cells purchased from Thermo Fisher Scientific (Cat # R75007) ...
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bioRxiv - Molecular Biology 2022Quote: Cells employed in the studies are HEK293 (Gibco 293-H cells), GM08207 (Coriell Cell Repositories ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293 FRT cells were cultured in zeocin (100 μg/ml, Invitrogen) to maintain the integrated FRT site ...
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bioRxiv - Neuroscience 2023Quote: HEK293 cells (ATCC) were cultured in Dulbecco’s Modified Eagles Medium (Gibco) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Biochemistry 2023Quote: Expi293F is a derivative of the HEK293 cell line (Thermo Fisher). Expi293F cells were grown in Expi293 Expression Medium (Thermo Fisher) ...
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bioRxiv - Biophysics 2023Quote: ... HEK293S GnTI-cells (ATCC) were cultured in Freestyle 293 medium (GIBCO) supplemented with 2% (v/v ...
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bioRxiv - Neuroscience 2023Quote: ... The siRNAs were transfected into HEK293 cells using Lipofectamine 2000 (Invitrogen). The effect of knockdown was validated by western blot.
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bioRxiv - Biochemistry 2023Quote: ... HEK293-F cells were grown in FreeStyle 293 expression medium (Gibco) (20 ...
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bioRxiv - Biochemistry 2023Quote: HEK293-F cells were grown in FreeStyle 293 Expression Medium (Gibco) at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: HEK293 cells were grown in Dulbecco’s modified Eagle medium (DMEM; Gibco) containing 10% (v/v ...
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bioRxiv - Physiology 2023Quote: HEK293 cells were cultured in Dulbecco’s modified Eagle’s medium (Gibco, France) supplemented with 10% fetal calf serum (Eurobio ...
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bioRxiv - Evolutionary Biology 2023Quote: ... HEK293 cells were washed with Opti-MEM Reduced Serum Media (Gibco) and incubated with transfection mix containing 1 µg total of the indicated plasmid DNA and 3 µL Lipofectamine 3000 Transfection Reagent (Invitrogen ...
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bioRxiv - Physiology 2023Quote: ... HEK293 cells were washed with Opti-MEM Reduced Serum Media (Gibco) and transfected using lipofectamine 2000 (Invitrogen/Life Technologies Cat #11668019 ...
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bioRxiv - Biochemistry 2023Quote: ... After transfection the Flp-In TRex HEK293 (Flp-In-293, Invitrogen) cells were selected in the presence of Hygromycin B (100 µg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 and C166 cells were maintained in DMEM (high glucose, Gibco) supplemented with 10% FBS (Giboco) ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293-FT (RRID:CVCL_6911) were culture similarly but in DMEM medium (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: HEK293-T cells were cultured in DMEM medium (#31966-021, Gibco) supplemented with 10% fetal bovine serum (#10270-106 ...
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bioRxiv - Neuroscience 2023Quote: HEK293 cells were grown in Dulbecco’s Modified Eagle Medium (DMEM, ThermoFisher) containing 10% fetal calf serum and 0.5% penicillin/streptomycin ...
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bioRxiv - Immunology 2021Quote: ... 100 units penicillin and 100 μg streptomycin per ml) supplemented with 50 U/ml IL-2 and 1 × 106 CD3/CD28-coated Human T-Activator Dynabeads/ml (Life Technologies). The next day ...
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bioRxiv - Immunology 2019Quote: ... The isolated CD8+ T cells were washed and resuspended at a concentration of 1 × 106 cells per ml in completely recon-stituted RPMI supplemented with 50 units/ml IL-2 and 1 × 106 CD3/CD28-coated Human T-Activator Dynabeads (Life Technologies) per ml ...
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bioRxiv - Immunology 2022Quote: ... Cells were incubated in RP-10 supplemented with 10 ng/ml recombinant human IL-2 and 2 μg/ml anti CD28 (clone 37.51, Thermo Fisher Scientific) for 18-22 hours ...
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bioRxiv - Microbiology 2022Quote: ... Primary human CD4+ T cells stimulated with IL-2 alone or with α-CD3/CD28 antibody-coated beads (Gibco/Thermo Fisher) were infected for 5 days ...
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bioRxiv - Microbiology 2022Quote: ... Primary human CD4+ T cells stimulated with IL-2 alone or with α-CD3/CD28 antibody-coated beads (Gibco/Thermo Fisher) were infected for 5 days ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 10% fetal bovine serum (FBS)) and 30IU/mL recombinant human IL-2 (rhIL-2; Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Immunology 2020Quote: ... The isolated CD8+ T cells were washed and resuspended at a concentration of 1 × 106 cells per ml in completely reconstituted RPMI supplemented with 50 units/ml IL-2 and 1 × 106 CD3/CD28-coated Human T-Activator Dynabeads (Life Technologies) per ml ...
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bioRxiv - Immunology 2020Quote: ... by negative selection magnetic bead sorting (Miltenyi) and activated in complete RPMI supplemented with 30 U/ml IL-2 with anti-human CD3/CD28 dynabeads (Thermo Fisher) at a 1:1 cell-to-bead ratio.
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bioRxiv - Immunology 2021Quote: Purified CD8+ cells were cultured in AIMV medium supplemented with 10% FBS and 100 U/ml recombinant human IL-2 (ThermoFisher Scientific) and used for sorting of CD8+ T cell subsets and for further experiments three days after isolation.
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bioRxiv - Immunology 2021Quote: ... populations were subject to either “rest” (low dose 30U/mL IL-2 only) or stimulation using Dynabeads Human T-Expander CD3/28 (ThermoFisher Scientific) at 1 million cells/mL at a ratio of 2 beads per cell for 48 hours ...
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bioRxiv - Immunology 2023Quote: ... as well as between stratified patient plasma samples (n=7) over three time points using an IL-23 Human ELISA Kit (BMS2023-3, ThermoFisher, Canada), as per manufacturer’s protocol ...
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Pathogenic CD8 T cell responses are driven by neutrophil-mediated hypoxia in cutaneous leishmaniasisbioRxiv - Immunology 2023Quote: ... 20 U/mL recombinant human IL-2 at 37°C and 5% CO2 for 24 hours in RPMI 1640 (Gibco, Canada) containing 100 Units of penicillin and 0.1 mg/mL of Streptomycin (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... IL-5 and IL-13 concentration were assessed following manufacturer’s instructions using mouse uncoated IL-5 and IL-13 ELISA kits (Invitrogen).
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bioRxiv - Immunology 2023Quote: ... IL-5 and IL-13 concentration were assessed following manufacturer’s instructions using mouse uncoated IL-5 and IL-13 ELISA kits (Invitrogen).
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bioRxiv - Cancer Biology 2020Quote: ... His-tagged GOT2 protein was immunoprecipitated using the His-tag isolation and pull-down Dynabeads system (Thermo Fisher) using the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and further ligated into pcDNA 3.1 V5/His TOPO vector using pcDNA 3.1 V5/His TOPO TA cloning kit (Invitrogen). The ACRD etoll was then transferred to pGADT7-AD vector using Nde1 and BamH1 sites ...
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bioRxiv - Cell Biology 2022Quote: ... The His SNX32ΔC protein bound to the lipids was detected by anti-His antibody [1:10,000 (Thermo Scientific)]
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bioRxiv - Molecular Biology 2023Quote: ... ceSMSγ and ceSMSr cDNAs were cloned into yeast expression vector pYES2.1/V5-His-TOPO and mammalian expression vector pcDNA3.1/V5-His-TOPO (Invitrogen) as described previously(18).For expression studies in Drosophila S2 cells ...
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bioRxiv - Microbiology 2023Quote: ... whereas His-tagged EF-P was detected with primary monoclonal antibodies against the His-Tag (1:10,000; AB_2536841, Invitrogen). Membranes were incubated with the secondary antibodies conjugated with a fluorophore (1:20,000 in TBS ...
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bioRxiv - Genetics 2021Quote: ... 12 µL Hi-Di Formamide (Applied Biosystems) and 0.3 µL MapMarker 500 labelled with DY-632 (Eurogentec) ...
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bioRxiv - Microbiology 2019Quote: ... we used Dynabeads™ His-Tag (Invitrogen) for Ni-affinity purification ...
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bioRxiv - Bioengineering 2022Quote: 6x-His tag antibodies (Invitrogen, MA1-21315) were coated at 2 µg/mL in PBS onto 96-well half- area high binding plates (Corning ...
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bioRxiv - Immunology 2021Quote: ... blot using an anti-His Ab (ThermoFisher). MSP-142 and AMA-1 were biotinylated and tetramerized with streptavidin-PE (SA-PE ...
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bioRxiv - Microbiology 2021Quote: ... eluted in Hi-Di formamide (Applied Biosystems) with ROX-350 ladder ...
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bioRxiv - Biochemistry 2021Quote: ... anti-His (MA1-21315, Invitrogen, 1:1,000). Proteins separated by SDS-PAGE were detected following the manufacturer’s guidelines (GE Healthcare ECL Western Blotting detection system ...
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bioRxiv - Microbiology 2020Quote: Hi-sensitivity Qubit DNA kit (Thermo Fisher) was used to measure the DNA concentration directly from the lysate ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% HI FBS (Gibco 10500), 20% D4T conditioned medium (24) ...
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bioRxiv - Microbiology 2020Quote: ... and cloned into pcDNA-V5/His (Invitrogen). The M fragment of CCHFV was PCR amplified with primers shown in Table II from cDNA generated from CCHFV genomic RNA ...