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Citations for Thermo Fisher :
201 - 250 of 7701 citations for IGSF11 Human HEK 293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: Human embryonic kidney (HEK) 293T cells were cultured in DMEM (GIBCO), 10% FBS ...
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bioRxiv - Bioengineering 2022Quote: Human embryonic kidney (HEK) 293T cells were cultured in DMEM (Gibco) supplemented with 10% FBS (Corning ...
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bioRxiv - Microbiology 2023Quote: ... murine TLR4 (mTLR4) and human TLR4 (hTLR4) HEK-Blue cells (Invitrogen), and RAW-Blue cells (Invitrogen) ...
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bioRxiv - Immunology 2024Quote: ... Human embryonic kidney (HEK) epithelial 293FT cells were ordered from Invitrogen. A549 cell lines were maintained in F-12K media with 10% fetal bovine serum (FBS) ...
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bioRxiv - Neuroscience 2024Quote: Human embryonic kidney (HEK) 293T cells were cultured in DMEM (GIBCO), 10% FBS ...
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bioRxiv - Systems Biology 2021Quote: ... anti-human FITC-IgG (Fc specific) (ThermoFisher Scientific) at a dilution of 1:400 and for 45 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: ... 100ug/mL anti-human IgG(Fc) (Life Technologies) was added to virus-Ab complexes and incubated for 30 min at 37°C prior to the addition of cells as described in (39) ...
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bioRxiv - Molecular Biology 2021Quote: ... dsiRNAs targeting Rpp30 (10 nM) were transfected into HEK 293 cells using Lipofectamine 3000 reagent according to the manufacturer’s instructions (Invitrogen). Target are available on www.idtdna.com ...
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bioRxiv - Neuroscience 2021Quote: ... 0.6 µg KV3.1b plasmid and 0.1 µg eGPF were transfected into HEK-293 cells using Lipofectamine 2000 (Invitrogen Corporation), and recordings were performed after 3 days of incubation at 37 °C ...
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bioRxiv - Biophysics 2020Quote: ... HEK-293 cells were transiently transfected with NaChBac WT or T220A cDNA using Lipofectamine™ 2000 transfection reagent (Invitrogen) and seeded onto 12 mm circular glass coverslips 24 hours prior to patch clamp recording ...
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bioRxiv - Neuroscience 2020Quote: ... expression vector plasmids encoding opsins or hrGFP were transfected into HEK 293 cells using Lipofectamine 2000 (Thermo Fisher Scientific). The cells were washed with phosphate-buffered saline (PBS ...
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bioRxiv - Physiology 2020Quote: ... 24 hours post-transfection, HEK 293 cell were harvested, resuspended in reduced serum media (MEM, NEAA (Thermo Fisher Scientific) supplemented with 1% L-glutamine (2 mM final ...
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bioRxiv - Synthetic Biology 2020Quote: ... human embryonic kidney 293 (HEK 293)(ATCC) cells were seeded in a 6-well plate at 0.3×106 cells/well in DMEM (ThermoFisher) supplemented with 10% FBS and 1% penicillin-streptomycin (Gibco ...
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bioRxiv - Neuroscience 2019Quote: Human embryonic kidney 293 (HEK 293) cells were cultured on 100 mm culture dishes in Dulbecco’s Modified Eagle’s medium (DMEM-GlutaMAX, Life Technologies) supplemented with 10% Foetal Calf Serum (FCS ...
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bioRxiv - Biochemistry 2021Quote: ... Plasmids (3 µg) were introduced into HEK 293 cells (1×106) by electroporation with Neon electroporator (Thermo Fisher Scientific) according to the manufacturers protocol ...
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bioRxiv - Genetics 2020Quote: ... Human embryonic kidney cells (HEK-293, ATCC) were grown in Dulbecco’s modified Eagle medium supplemented with 10% fetal bovine serum (Gibco). Cell lines were regularly screened for mycoplasma contamination by DAPI staining ...
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bioRxiv - Biophysics 2022Quote: ... HEK 293 cells and COS-7 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Thermo Fisher Scientific, USA) supplemented with glucose (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... HEK-293 cells were purchased from ATCC and cultured in 10% (v/v) FBS (Gibco, Cat. No. 10270-106) and 100U mL-1 penicillin and 100µg mL-1 streptomycin (Gibco ...
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bioRxiv - Biochemistry 2023Quote: ... HEK-293 cells were purchased from ATCC and maintained in 10% (v/v) FBS (Gibco, Cat. no. 10270-106) and 100U ml-1 penicillin and 100 µg ml-1 streptomycin (Gibco ...
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bioRxiv - Molecular Biology 2024Quote: ... in a pcDNA5/FRT/TO/n2SC or pcDNA5/FRT/TO/c2SC vector with 0.9 µg of pOG44 plasmid into HEK 293 Flp-In T-REx cells (Invitrogen). Cells were selected for 2 weeks in DMEM containing 100 µg/mL hygromycin and 15 µg/mL blasticidin ...
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bioRxiv - Biochemistry 2024Quote: ... HEK-293 cells were purchased from ATCC and maintained in 10% (v/v) FBS (Gibco, Cat. no. 10270-106) and 100 U ml-1 penicillin and 100 µg ml-1 streptomycin (Gibco ...
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bioRxiv - Developmental Biology 2023Quote: HEK-293 (ATCC CRL-1573) was maintained in DMEM supplemented with 10% fetal bovine serum and penicillin/streptomycin (Invitrogen). Cells were cultured according to the manufacturer’s instructions (ATCC) ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293T (Human Embryonic Kidney cells 293) cells were obtained from Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... the expression vector was used to transfect human 293-EBNA cells (Invitrogen) and serum- free medium was collected for protein purification according to established methods [51] ...
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bioRxiv - Molecular Biology 2022Quote: Human embryonic kidney (HEK293; Invitrogen’s Flp-In-293 cell line cat #R75007) cells were grown and maintained in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Biochemistry 2022Quote: HEK293 (Human Embryonic Kidney 293-Flp-In T-Rex, Thermo Fisher Scientific) cells were cultured in DMEM (Dulbecco’s modified Eagle‘s medium ...
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bioRxiv - Biochemistry 2021Quote: ... Human embryonic kidney 293T cells (HEK-293T) were cultured in DMEM (Gibco) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Cell Biology 2021Quote: Suspension cultures of human embryonic kidney (HEK) 293F cells (purchased from Invitrogen) were maintained using 293 freestyle media (gibco ...
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bioRxiv - Neuroscience 2019Quote: Human embryonic kidney (HEK) 293T cells were cultured in DMEM (GIBCO 11995065), 10% FBS (Gibco ILT10082147) ...
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bioRxiv - Microbiology 2019Quote: ... we grew human embryonic kidney (HEK) 293FT cells (Thermo Fisher Scientific; #R70007) in Dulbecco’s modified Eagle medium (DMEM ...
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bioRxiv - Cell Biology 2023Quote: ... Pooled neonatal primary Human Epidermal Keratinocytes (HEK) were obtained from Life Technologies and were used between passages two to five ...
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bioRxiv - Neuroscience 2023Quote: ... Human fibroblasts and Human embryonic kidney (HEK) 293T cells were cultured in DMEM (1g/L glucose) (GIBCO) supplemented with 10% Fetal Calf Serum (FCS) ...
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bioRxiv - Cancer Biology 2021Quote: ... was then gently mixed with 20 parts (v/v) of HEK-293 cells (ATCC) suspended at a density of 2 x 105 cells/mL in DMEM (Gibco) + 10% FBS (Seradigm/VWR) ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... HEK-293 cells were transferred with a density of 12000 cells per well in white 96-well plates (Thermo Scientific™ Nunc™ F96 MicroWell™ ...
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bioRxiv - Biochemistry 2020Quote: ... HEK 293T and T-Rex 293 cells were grown in Dulbecco’s modified Eagles medium (DMEM, Thermo Fisher scientific, MA, USA) containing 4.5 mg/ml glucose ...
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bioRxiv - Neuroscience 2020Quote: ... Lentiviruses were produced by transfecting HEK 293 cells with lentiviral vectors together with packaging vectors (3rd generation) using Lipofectamine 3000 (Thermofisher). Viral supernatant was collected 24hr and 48hr post-transfection ...
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bioRxiv - Genetics 2022Quote: HEK 293 cells were cultured in medium consisting of high glucose DMEM (Hyclone) supplemented with 10% fetal bovine serum (Gibco) and 1× penicillin-streptomycin (Gibco) ...
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bioRxiv - Microbiology 2020Quote: ... The pSAd36-S and pSAd-control plasmids were linearized with PacI restriction enzyme to liberate viral genomes for transfection into T-Rex 293-HEK cells (Invitrogen). The rescued replication-incompetent ChAd-SARS-CoV-2-S and ChAd-Control vectors were scaled up in 293 cells and purified by CsCl density-gradient ultracentrifugation ...
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bioRxiv - Microbiology 2022Quote: The viability of HEK-293 cells post exposure to films was also determined using a LIVE/DEAD Viability/Cytotoxicity Kit (Invitrogen). Approximately 105 cells were seeded per well ...
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bioRxiv - Molecular Biology 2022Quote: pcDNA5/FRT/TO-FLAG-Angel1 WT was used to generate stable HEK FLp-In T-REx-293 cell lines (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... supplemented with 10% Foetal Bovine Serum (FBS) (HEK 293) or Calf Serum (NIH 3T3 and Ptch1−/− MEFs) containing 1% Glutamax (Gibco). All cell lines were grown at 37°C in 5% CO2 and passaged prior to reaching confluence.
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bioRxiv - Cancer Biology 2023Quote: ... HEK-293 (CRL-1573) cells were obtained from ATCC and maintained in high-glucose (4.5 g/L) DMEM (Gibco 11995073) supplemented with 4 mM L-glutamine ...
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bioRxiv - Microbiology 2023Quote: ... lentiviruses (for target sequences see S1 File) for shRNA delivery were produced by transfecting HEK-293 cells in a 60mm dish with a 1ml OptiMEM (Gibco) mix containing 15µl of Lipofectamine-2000 (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... with fewer than 15 passages onto 12-well plates in HEK-293 growth medium (DMEM [Gibco # 10566-061] with 10% FBS [Gibco #16140-071] and 1x Pen Strep [Gibco #15070-63] ...
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bioRxiv - Neuroscience 2023Quote: ... we plated HEK-293 cells (ATCC catalog # CRL-1573) with fewer than 15 passages onto 12-well plates in HEK-293 growth medium (DMEM [Gibco # 10566-061] with 10% FBS [Gibco #16140-071] and 1x Pen Strep [Gibco #15070-63] ...
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bioRxiv - Biophysics 2024Quote: The original CC1 construct with super ecliptic pHluorin (SE) A227D (31) or CC1 variants with mutations in S1 were expressed in HEK 293 cells via lipofectamine (Invitrogen). Coverslips with transiently transfected cells were placed into a bath chamber (Warner instruments ...
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bioRxiv - Biophysics 2020Quote: ... and inserted into a human Fc expression vector (Invitrogen). The proteins were expressed in HEK293T cells ...
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bioRxiv - Biochemistry 2022Quote: ... Human FLP-In™ T-REx™ 293 Cell Lines (Invitrogen™, R78007) expressing hnRNPA1 wild type or D262V mutant cDNAs were generated by cotransfecting the hnRNPA1 cDNAs with a FLP recombinase expression plasmid according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... and transfected into human Flp-In T-REx 293 cells (Invitrogen, Carlsbad, CA). Following hygromycin selection and cell expansion ...
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bioRxiv - Cell Biology 2020Quote: ... Identified and characterized plasmids containing the ACE2 isoform open reading frames were transfected into the HEK-293 cells using TurboFect reagent (ThermoFisher Corp.). 24 hours post-transfection the cells were harvested and processed for analysis by western blot according to established procedures [21] ...