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Citations for Thermo Fisher :
401 - 450 of 3033 citations for IFN gamma Rat CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Rat astrocytes (Gibco™, N7745100) were consecutively seeded at a density of 100 cells/mm2 ...
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bioRxiv - Neuroscience 2022Quote: ... -anti-rat 568 (Invitrogen, #A11004) were used 1:1000 dilution as secondary antibody ...
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bioRxiv - Neuroscience 2023Quote: ... Rat mCherry 1:1000 (Invitrogen)) ...
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bioRxiv - Neuroscience 2023Quote: ... rat mCherry (1:1000) (Invitrogen), mouse puromycin (1:1000 ...
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bioRxiv - Bioengineering 2023Quote: ... rat tail (A1048301, Thermo Fisher); Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Cell Biology 2022Quote: ... anti-rat AF488 (ThermoFisher A21208)) ...
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bioRxiv - Microbiology 2022Quote: ... anti-rat-HRP (Invitrogen, #31470), anti-sheep-HRP (A16041 ...
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bioRxiv - Neuroscience 2023Quote: ... Anti-Rat AF 568 (Invitrogen), Anti-Rabbit AF 647 (Invitrogen)) ...
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bioRxiv - Developmental Biology 2023Quote: ... anti-rat AF488 (Invitrogen, A21208); anti-goat AF488 (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... anti-rat Alexa Fluor546 (Invitrogen) and anti-guinea pig Cy3 (Jackson ImmunoResearch Inc.).
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bioRxiv - Developmental Biology 2023Quote: ... EpCAM (rat, 1:100, Invitrogen), Sca-1 (rat ...
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bioRxiv - Immunology 2024Quote: ... Goat anti-Rat Alexa555 (Invitrogen); and Goat anti-Rat Alexa647 (Invitrogen) ...
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bioRxiv - Neuroscience 2024Quote: ... Rat-GFAP (1:1000, Thermofisher), Rabbit-GFAP (1:1000 ...
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bioRxiv - Immunology 2020Quote: ... pH 7.2) resuspended and incubated for 30 min in FACS buffer supplemented with Fc gamma receptor CD16/CD32 antibodies (ThermoFisher). Cells were then incubated with Alexa fluor 488-conjugated antibodies against F4/80 or rat IgG2a kappa isotype control ...
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bioRxiv - Immunology 2024Quote: ... corresponding to the concentration of the highest standard of recombinant human IFNγ (Human IFNγ Gamma Uncoated ELISA kit, Invitrogen). IFNγ concentrations below the level of detection by the ELISA standard curve were set to 0 pg/ml ...
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bioRxiv - Developmental Biology 2019Quote: cDNA encoding FLAG-CT-mVenus of rat CACNA1C and rat AKAP2 was purchased from Thermofisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... GM was replaced with GM containing 64 different IFN-γ and TNF-α (Life Technologies) priming conditions consisting of a full factorial design of 0 ...
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bioRxiv - Immunology 2020Quote: ... IL-17 and IFN-γ were measured by ELISA Ready-Set-Go kits from Thermofisher and culture supernatants were tested at different dilutions according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: Patient sera were thawed and used as per manufacturer’s protocols for IFN-α ProQuantum (Invitrogen), LEGENDMAX MIF ELISA (BioLegend ...
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bioRxiv - Biophysics 2021Quote: A stable β2AR-overexpressing cell line was produced by transfecting CHO-K1 cells (ECACC) with a plasmid coding for the human β2AR with C-terminal GFP using lipofectamine 2000 (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... cells stably expressing I-Ek-MCC (CHO-I-Ek) were maintained by passage every 3 days in complete RPMI 1640 medium (ThermoFisher Scientific ...
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bioRxiv - Genetics 2019Quote: ... ExpiCHO-S cells were transfected with full-length pRTL1-GCSF vectors using the ExpiFectamine™ CHO Transfection Kit (Life Technologies Corporation ...
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bioRxiv - Genetics 2021Quote: ... The hGOT1-GFP and mutant hGOT1-GFP constructs were transfected into Chinese Hamster Ovary (CHO) (ExpiCHO-S) cells with an ExpiFectamine Transfection Kit (Gibco) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... were transfected with the appropriate light chain and heavy chain encoding plasmids for each Fab following the ExpiFectamine CHO Transfection Kit (ThermoFisher) high titer protocol ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids containing the Fab heavy chains and light chains were chemically co-transfected into an afucosylated ExpiCHO cell line using the ExpiFectamine CHO Transfection Kit (Gibco). After 9 days of transient expression ...
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bioRxiv - Biophysics 2022Quote: ... were transfected with the appropriate light chain and heavy chain encoding plasmids for each Fab following the ExpiFectamine CHO Transfection Kit (ThermoFisher) high titer protocol ...
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bioRxiv - Biochemistry 2021Quote: The wild-type (WT) and mutant GLP-1R constructs were integrated into FlpIn-CHO cells using FlpIn Gateway technology system (Invitrogen). Stable CHOFlpIn expression cell lines were selected using 600 μg/ml hygromyocin B ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Stable Flp-In-CHO cell lines were generated through co-transfection of the pcDNA5/FRT expression vector (Thermo Fisher Scientific) containing the gene of interest and the Flp recombinase expressing plasmid ...
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bioRxiv - Microbiology 2021Quote: Plasmid pCAG-CD5-S-ecto-6P-Spytag was transiently transfected into ExpiCHO cells using ExpiFectamine CHO transfection kit (Thermo Fisher). After 18-22 hr of transfection ...
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bioRxiv - Cell Biology 2022Quote: Histidine-tagged Mindin was purified from conditioned media collected from CHO-Mindin cells using Ni-NTA beads (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2019Quote: ... consisting of 43,856 probe sets that correspond to 13,358 mouse genes based on CHO EST Sequence and Annotation Release 06 (Affymetrix, Singapore). Quality assessment and data analysis were carried out on the CEL files obtained ...
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bioRxiv - Neuroscience 2019Quote: ... were transfected into HEK293T cells (3×105/well) using PEI or into CHO cells (1.5×105/well) with lipofectamine 3000 reagent (ThermoFisher, L3000001) in 24-well plates ...
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bioRxiv - Microbiology 2020Quote: ... HEK293 and CHO cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12; Gibco, #11320033), supplemented with 10% (w/v ...
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bioRxiv - Microbiology 2021Quote: CHO cells stably expressing SARS-CoV-2 S-glycoprotein were seeded in 96 well plates for microscopy (Thermo Fisher Scientific) at 12’500 cells/well and the following day ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: CHO cell lines overexpressing FPR2 and FPR1 receptors were generated in-house and propagated in Ham’s F12 medium (Gibco Biosciences) supplemented with 10% (v/v ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Total RNA from CHO-FPR2 overexpressing cells or HL-60 cells was isolated using Trizol reagent (Invitrogen, Carlsbad, CA, USA) and purified using RNeasy Mini kits (Qiagen ...
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bioRxiv - Immunology 2020Quote: ... were transfected with the plasmid expressing S-2P protein ectodomains by ExpiFectamine 293 transfection kit and ExpiFectamine CHO transfection kit (ThermoFisher), respectively ...
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bioRxiv - Biophysics 2022Quote: ... and a combination of the glCV30 Hc and glCV30 Lc vectors or a combination of the glCV30-SDF-1α Hc and glCV30 Lc vectors were introduced into the CHO-S cells (Gibco). The transfected cells were grown in the ExpiCHO expression medium (Gibco ...
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bioRxiv - Physiology 2023Quote: Myc-tagged wild-type (WT) and PKA-resistant mutant (Ser791Ala) Raptor vectors7 were transfected into CHO-GLP-1R cells using lipofectamine-3000 (Invitrogen). 48h later ...
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bioRxiv - Immunology 2022Quote: ... expansion and production of the CHO cells from the working cell bank (WCB) into shake flasks and bioreactor (Applikon) using serum-free medium (Gibco). At the end of the growth phase ...
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bioRxiv - Immunology 2023Quote: ... CHO and G8 hybridomas cells were loaded respectively with 10 μM of the red cell-labeling agent DDAO (Molecular Probes) [ex/em 635/662] and 1 μM Vybrant CFDA SE [ex/em 492/517] in serum-free RPMI-1640 for 30 minutes at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... CHO-DSP1 and MeWo-DSP2 cells were kept under puromycin selection (8 µg/ml for CHO-DSP1 and 5 µg/ml for MeWo-DSP2; Gibco). BHK-21 cells were grown in GMEM supplemented with 20 mM Hepes pH 7.4 ...
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bioRxiv - Neuroscience 2024Quote: Chinese Hamster Ovary (CHO) cells were maintained similar to HEK cells with the following differences: Culture media was IMDM (Invitrogen) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2020Quote: ... the macrophages were allowed to adhere to the culture plate for 20 minutes before 100 units (U) of murine recombinant interferon-gamma (IFNγ) (Invitrogen) was added to each well to stimulate the macrophages ...
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bioRxiv - Immunology 2022Quote: Cytokine and chemokine levels in gamma irradiated (2 megarads) BAL specimens were analyzed using a custom ProcartaPlex immunoassay (ThermoFisher Scientific) nonhuman primate cytokine magnetic bead panel premixed 24-plex kit ...
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bioRxiv - Neuroscience 2023Quote: ... and/or mCherry (primary: rat anti-mCherry, Invitrogen #M11217; secondary: goat anti-rat 594, Invitrogen #11007). Primary antibodies were used at a concentration of 1:1000 and incubated on a shaker overnight at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Toxicogenomic data from the TG-GATEs (TG) rat kidney repository (Affymetrix Rat Genome 230 2.0 array) were downloaded (Igarashi et al. ...
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bioRxiv - Developmental Biology 2021Quote: ... goat-anti-rat-Alexa488 (Life Technologies) diluted to 1:1000 in PBS and 0.2% Triton X-100.
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bioRxiv - Evolutionary Biology 2021Quote: ... rat anti-OLLAS clone L2 (Invitrogen) 1:200 ...
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bioRxiv - Developmental Biology 2021Quote: ... donkey anti-rat Alexa488 (Invitrogen A21208), goat anti-rat Alexa546 (1:500 ...