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Citations for Thermo Fisher :
201 - 250 of 10000+ citations for IFN γ Receptor II Human CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... CHO cells were transfected using Lipofectamine 2000 reagent (ThermoFisher) and cells stably-expressing each construct were selected-for with blasticidin ...
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bioRxiv - Bioengineering 2022Quote: ... were grown in Freestyle CHO expression medium (ThermoFisher Scientific); suspension HEK293 host cell line (Freestyle 293-F ...
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bioRxiv - Neuroscience 2023Quote: ... CHO-K1cells were cultured in DMEM/F12 Medium (Gibco) supplemented with 10% FBS and penicillin/streptomycin (10 U/ml and 10 µg/ml ...
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bioRxiv - Immunology 2023Quote: ... Transfections were carried out using CHO Freestyle cells (Invitrogen). 1.24mg of plasmid DNA was transfected into 90 million cells were using a MaxCyte Electroporation Transfection System ...
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bioRxiv - Microbiology 2024Quote: ... CHOs were transfected using Lipofectamine 3000 transfection reagent (Invitrogen) according to manufacturer instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Flip-InTM-CHO cells (purchased from Thermo Fisher Scientific), was cultured in Ham’s F-12 medium (Sigma–Aldrich ...
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bioRxiv - Neuroscience 2019Quote: Silencing lentiviral vectors were produced by co-transfecting HEK293T producing cells with lentiviral silencing plasmids GIPZ Human histamine H3 receptor shRNA (Clone V3LHS_638095 or Clone V3LHS_638091, Thermo Scientific) with packing plasmid psPAX2 and envelope coding plasmid pMD2.G (Addgene#12260 and #12259 ...
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bioRxiv - Neuroscience 2023Quote: ... Transferrin (loading control) was detected with mouse anti-human transferrin receptor antibody (1:500, Cat# 136800, Invitrogen, Waltham, MA). Protein samples (50 µg ...
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bioRxiv - Immunology 2021Quote: - IFNγ and IL-10 levels were measured in the supernatant of the WBA using IFN gamma and IL-10 Human ELISA Kit (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: ... and anti-transferrin receptor (Invitrogen). For the screening of compounds with the potential to rescue GlyT2 defective phenotypes the following chemicals were used ...
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bioRxiv - Cell Biology 2023Quote: ... Transferrin receptor (Invitrogen, 13-6890), NHE5 (PA5-37222) ...
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bioRxiv - Microbiology 2021Quote: The IFN-β levels in BALF were determined using mouse IFN beta ProQuantum Immunoassay kit (ThermoFisher). IFN-α and IFN-β levels in the supernatants of cultured BMDCs were determined by ELISA (PBL Biomedical Laboratories).
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bioRxiv - Immunology 2019Quote: ... The IFN-α/IFN-β 2-Plex Mouse ProcartaPlexTM Luminex Panel kit used was from Invitrogen. Tetramethylrhodamine ...
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bioRxiv - Cell Biology 2020Quote: Parent CHO-K1 and CHO-K1 cells stably expressing extracellular ACP-tagged human insulin receptors were maintained at 37°C with 5% CO2 in HAM’s F-12 medium (Thermo Fisher) supplemented 10% fetal bovine serum (FBS) ...
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bioRxiv - Immunology 2021Quote: The COVID-19 receptor-binding domain (RBD) and the N-terminal peptidase domain of human ACE2 were expressed using HEK293F cells (Invitrogen). The COVID-19 RBD (residues Arg319-Phe541 ...
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bioRxiv - Microbiology 2022Quote: ... Cells were seeded in 96-well-plates and treated with 10μg/ml OM-85 concentrate (OM-Pharma) or 100 ng/mL of human recombinant IFN-β protein (ThermoFisher Scientific). The concentration of 10μg/ml of OM-85 was based on previous data with RSV we recently generated [9] ...
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bioRxiv - Microbiology 2021Quote: ... the bound antibody was detected with goat anti-human Ig (γ chain specific)-HRP conjugated secondary antibody (Thermo Fisher Scientific) diluted 1:10,000 in blocking buffer ...
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bioRxiv - Immunology 2021Quote: ... ExpiFectamine CHO Enhancer and ExpiCHO Feed reagents (Thermo Fisher Scientific) were added to the cells ...
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bioRxiv - Systems Biology 2019Quote: Two isogenic CHO-S cell lines (Thermo Fisher Scientific, USA) adapted to grow in suspension ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... were transfected into the Flp-In-CHO cell line (ThermoFisher) as previously described (22) ...
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bioRxiv - Systems Biology 2021Quote: ... was used for Freestyle™ CHO-S cells (Gibco™). Cells were cultivated in 125 ml Erlenmeyer shake flasks at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... CHO cells were harvested and loaded with Coelenterazine H (Invitrogen) for 4 h at room temperature ...
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bioRxiv - Bioengineering 2021Quote: CHO cells stained with 20 mM CFDA-SE dye (Invitrogen,) were incubated at 37°C for 20 minutes ...
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bioRxiv - Cell Biology 2021Quote: CHO cells detached using Hyclone HyQTase (Thermo Fisher Scientific, SV300.30.01), washed with Tyrode’s Buffer (10 mM HEPES-NaOH ...
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bioRxiv - Immunology 2022Quote: ... single CHO-cells using an Attune NxT 6 cytometer (ThermoFisher).
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bioRxiv - Microbiology 2021Quote: ... were transfected with 640 μl of Expifectamine CHO reagent (ThermoFisher) and 200 μg of CCoV-HuPn-2018-S-2P following manufacturer’s recommendations ...
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bioRxiv - Immunology 2021Quote: ... ExpiFectamine CHO Enhancer and ExpiCHO Feed reagents (Thermo Fisher Scientific) were added to the cells ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The cells were transfected with ExpiFectamine CHO (Thermo Fisher Scientific) according to the manufacturer’s directions ...
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bioRxiv - Immunology 2020Quote: ... ExpiFectamine CHO Enhancer and ExpiCHO Feed reagents (Thermo Fisher Scientific) were added to the cells ...
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bioRxiv - Immunology 2021Quote: CHO cells (cGMP bank, Thermo Fisher Scientific, Waltham, MA, USA) were propagated and maintained in the animal component free ...
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bioRxiv - Bioengineering 2022Quote: ... Austria) were grown either in CD CHO medium (ThermoFisher Scientific) or CHOlean (Xell AG ...
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bioRxiv - Bioengineering 2022Quote: ... CHO-bsAb cell line was maintained in Dynamis medium (Gibco) supplemented with 4 mM glutamine ...
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bioRxiv - Microbiology 2023Quote: Expi-CHO-S Cells (Cat# A29127; ThermoFisher Scientific, Waltham MA) were cultured in ExpiCHO Expression Medium (Cat# A2910001 ...
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bioRxiv - Cancer Biology 2023Quote: ... Flp-InTM CHO cells (Thermo Fisher Scientific, Waltham, MA, USA) stably overexpressing mouse PAMR1 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Chinese hamster ovary (CHO) FlpIn cells were purchased from Invitrogen, Fetal bovine serum (FBS ...
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bioRxiv - Immunology 2024Quote: ... following the high-titer protocol for CHO Expifectamine (ThermoFisher Scientific). Cultures were centrifuged 9–10 days after transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... ExpiFectamine CHO Enhancer and ExpiCHO Feed reagents (Thermo Fisher Scientific) were added to the cells ...
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bioRxiv - Cell Biology 2024Quote: FlpIn CHO and HEK293 cell lines were purchased from Invitrogen and maintained in Dulbecco’s Modified Eagle’s Medium supplemented with 5% Foetal Bovine Serum (ThermoFisher Scientific (Gibco) ...
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bioRxiv - Neuroscience 2023Quote: ... Fetal brains were evaluated by Western blot (corticotropin releasing factor receptor 1 [CRFR1]; glucocorticoid receptor [GR], interleukin [IL]-6 receptor [IL-6R]; IL-17A and β-actin, Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Cell Biology 2021Quote: The expression cassette consisting of a Kozak sequence followed by the coding sequence for human diphtheria toxin receptor (hDTR; UniProt Q53H93) was codon-usage optimized for expression in mice and synthesized (Thermo Fisher Scientific/GeneArt ...
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bioRxiv - Cell Biology 2021Quote: ... gene expression was analysed using the TaqMan™ Array Human Cardiomyocyte Differentiation by BMP Receptors (Thermo Fisher Scientific, Waltham, MA). Methods are detailed in the online Supplementary material.
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bioRxiv - Molecular Biology 2020Quote: ... and Transferrin Receptor (1:1,000; Invitrogen). Proteins were detected with HRP-conjugated secondary antibodies (1:10,000 ...
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bioRxiv - Neuroscience 2023Quote: ... anti‐transferrin receptor (1:500; Invitrogen), anti‐α‐fodrin (1:5000 ...
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bioRxiv - Microbiology 2023Quote: Levels of IFN-λ in the culture supernatants were determined with ELISA using the human IL-29 ELISA kit (Invitrogen, cat. BMS2049) following the instructions of the manufacturers ...
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bioRxiv - Microbiology 2020Quote: ... HRP conjugate (1/4500) (all from Jackson Immunosearch Laboratories, Inc.) or goat anti-human IgG (γ-chain specific) HRP conjugate (1/50 000) (Invitrogen) were added and samples were incubated at 20-24°C for 60 minutes ...
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bioRxiv - Genomics 2020Quote: ... 5% A+ human serum (Interstate Blood-Bank) and 0.5% AlbuMAX II (Life Technologies), 5% HC ...
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bioRxiv - Immunology 2022Quote: ... IFN-alpha rabbit polyclonal (#PA5-115430 ThermoFisher). CD123-goat polyclonal (#ab205365 Abcam ...
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bioRxiv - Biophysics 2022Quote: ... CHO-K1 cells constitutively expressing human KCNE1 (designated CHO-KCNE1 cells) were generated as previously described (74) using the FLP-in™ system (Thermo Fisher Scientific, Waltham, MA, USA) and maintained under selection with hygromycin B (600 μg/mL).
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bioRxiv - Developmental Biology 2019Quote: ... anti-γ-tubulin (1:200, Invitrogen), anti-aPKC (1:200 ...
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bioRxiv - Cell Biology 2022Quote: ... and γ Tubulin (4D11, Thermo Scientific) antibodies.