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Citations for Thermo Fisher :
501 - 550 of 10000+ citations for IFN γ Receptor II Human CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Cyanine3 or Alexa-488 labeled polyclonal γ-tubulin antibody (1:1000) (Thermo Fisher) and Alexa Fluo 488nm labeled acetylated α-tubulin antibody (1:500 ...
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bioRxiv - Microbiology 2022Quote: ... and 5’-labeled with [32P]-γ-ATP using T4 polynucleotide kinase (Thermo Scientific) with forward reaction buffer according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... Nuclear and poly-γ-glutamate were stained with DAPI (1:250; Invitrogen, D1306), and actin fibers were stained with Alexa 546 phalloidin (1:400 ...
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bioRxiv - Microbiology 2024Quote: ... and human (goat anti-human IgG, ThermoFisher) were also used ...
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bioRxiv - Immunology 2023Quote: Patient sera were thawed and used as per manufacturer’s protocols for IFN-α ProQuantum (Invitrogen), LEGENDMAX MIF ELISA (BioLegend ...
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bioRxiv - Cell Biology 2022Quote: ... cells stably expressing I-Ek-MCC (CHO-I-Ek) were maintained by passage every 3 days in complete RPMI 1640 medium (ThermoFisher Scientific ...
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bioRxiv - Genetics 2019Quote: ... ExpiCHO-S cells were transfected with full-length pRTL1-GCSF vectors using the ExpiFectamine™ CHO Transfection Kit (Life Technologies Corporation ...
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bioRxiv - Genetics 2021Quote: ... The hGOT1-GFP and mutant hGOT1-GFP constructs were transfected into Chinese Hamster Ovary (CHO) (ExpiCHO-S) cells with an ExpiFectamine Transfection Kit (Gibco) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... were transfected with the appropriate light chain and heavy chain encoding plasmids for each Fab following the ExpiFectamine CHO Transfection Kit (ThermoFisher) high titer protocol ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids containing the Fab heavy chains and light chains were chemically co-transfected into an afucosylated ExpiCHO cell line using the ExpiFectamine CHO Transfection Kit (Gibco). After 9 days of transient expression ...
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bioRxiv - Biophysics 2022Quote: ... were transfected with the appropriate light chain and heavy chain encoding plasmids for each Fab following the ExpiFectamine CHO Transfection Kit (ThermoFisher) high titer protocol ...
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bioRxiv - Biochemistry 2021Quote: The wild-type (WT) and mutant GLP-1R constructs were integrated into FlpIn-CHO cells using FlpIn Gateway technology system (Invitrogen). Stable CHOFlpIn expression cell lines were selected using 600 μg/ml hygromyocin B ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Stable Flp-In-CHO cell lines were generated through co-transfection of the pcDNA5/FRT expression vector (Thermo Fisher Scientific) containing the gene of interest and the Flp recombinase expressing plasmid ...
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bioRxiv - Microbiology 2021Quote: Plasmid pCAG-CD5-S-ecto-6P-Spytag was transiently transfected into ExpiCHO cells using ExpiFectamine CHO transfection kit (Thermo Fisher). After 18-22 hr of transfection ...
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bioRxiv - Cell Biology 2022Quote: Histidine-tagged Mindin was purified from conditioned media collected from CHO-Mindin cells using Ni-NTA beads (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2019Quote: ... consisting of 43,856 probe sets that correspond to 13,358 mouse genes based on CHO EST Sequence and Annotation Release 06 (Affymetrix, Singapore). Quality assessment and data analysis were carried out on the CEL files obtained ...
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bioRxiv - Neuroscience 2019Quote: ... were transfected into HEK293T cells (3×105/well) using PEI or into CHO cells (1.5×105/well) with lipofectamine 3000 reagent (ThermoFisher, L3000001) in 24-well plates ...
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bioRxiv - Microbiology 2020Quote: ... HEK293 and CHO cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12; Gibco, #11320033), supplemented with 10% (w/v ...
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bioRxiv - Microbiology 2021Quote: CHO cells stably expressing SARS-CoV-2 S-glycoprotein were seeded in 96 well plates for microscopy (Thermo Fisher Scientific) at 12’500 cells/well and the following day ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Total RNA from CHO-FPR2 overexpressing cells or HL-60 cells was isolated using Trizol reagent (Invitrogen, Carlsbad, CA, USA) and purified using RNeasy Mini kits (Qiagen ...
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bioRxiv - Immunology 2020Quote: ... were transfected with the plasmid expressing S-2P protein ectodomains by ExpiFectamine 293 transfection kit and ExpiFectamine CHO transfection kit (ThermoFisher), respectively ...
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bioRxiv - Biophysics 2022Quote: ... and a combination of the glCV30 Hc and glCV30 Lc vectors or a combination of the glCV30-SDF-1α Hc and glCV30 Lc vectors were introduced into the CHO-S cells (Gibco). The transfected cells were grown in the ExpiCHO expression medium (Gibco ...
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bioRxiv - Physiology 2023Quote: Myc-tagged wild-type (WT) and PKA-resistant mutant (Ser791Ala) Raptor vectors7 were transfected into CHO-GLP-1R cells using lipofectamine-3000 (Invitrogen). 48h later ...
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bioRxiv - Immunology 2022Quote: ... expansion and production of the CHO cells from the working cell bank (WCB) into shake flasks and bioreactor (Applikon) using serum-free medium (Gibco). At the end of the growth phase ...
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bioRxiv - Immunology 2023Quote: ... CHO and G8 hybridomas cells were loaded respectively with 10 μM of the red cell-labeling agent DDAO (Molecular Probes) [ex/em 635/662] and 1 μM Vybrant CFDA SE [ex/em 492/517] in serum-free RPMI-1640 for 30 minutes at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... CHO-DSP1 and MeWo-DSP2 cells were kept under puromycin selection (8 µg/ml for CHO-DSP1 and 5 µg/ml for MeWo-DSP2; Gibco). BHK-21 cells were grown in GMEM supplemented with 20 mM Hepes pH 7.4 ...
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bioRxiv - Neuroscience 2024Quote: Chinese Hamster Ovary (CHO) cells were maintained similar to HEK cells with the following differences: Culture media was IMDM (Invitrogen) supplemented with 10% FBS ...
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bioRxiv - Biochemistry 2021Quote: ... CellfectinTM II (ThermoFisher) was employed to transfect 10 μg of bacmid into 1×106 attached Sf9 cells following manufacturer’s protocols (ThermoFisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... SuperScript II (Invitrogen) was added and the reaction continued at 42°C for 15 min ...
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bioRxiv - Cancer Biology 2023Quote: ... dispase II (Gibco), and DNaseI (Roche ...
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bioRxiv - Cancer Biology 2020Quote: ... B lymphocytes were obtained by negative cell selection using the MagniSort Human B-cell enrichment kit II (Thermo Fisher Scientific, Carlsbad, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: Full-length human SETD7 cDNA sequences were amplified from a human mRNA pool generated by RT-PCR using SuperScript II RNase H Reverse Transcriptase (Life Technologies,CA, USA). The SETD7 cDNA was cloned into the EcoRI/BamHI site of a pCDH-CMV-MCS-EF1α -Puro vector (System Biosciences ...
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bioRxiv - Immunology 2022Quote: ... and binding to Fc receptors was blocked using CD16/CD32 (clone 93, eBioscience/ThermoFisher). Cell numbers were calculated using counting beads (123count eBeads ...
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bioRxiv - Bioengineering 2021Quote: ... cells were blocked with an anti-Fc Receptor polyclonal antibody (Invitrogen 14-9161-73) for 30 minutes ...
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bioRxiv - Bioengineering 2020Quote: ... while acetylcholine receptors were detected by a-bungarotoxin (Alexa Fluor 488®, Invitrogen # B13422). Nuclei were stained with 300 nM DAPI (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... mouse monoclonal anti-transferrin receptor (clone H68.4 catalog no. 13-6800, Thermo-Fisher Scientific); mouse monoclonal anti-tubulin (clone DM1A ...
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bioRxiv - Physiology 2019Quote: ... Protein homogenates were analyzed for abundance of phosphorylated(Tyr972)-insulin receptor (Invitrogen: 44-800G), insulin receptor-β (Cell Signaling ...
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bioRxiv - Cancer Biology 2021Quote: ... rabbit anti-GABA A Receptor α6 (1:1000 dilution; Cat. #. PA5-77403, GABRA6; Invitrogen), and a-Tubulin (1:5000 dilution ...
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bioRxiv - Immunology 2020Quote: ... Fc receptors were blocked prior to staining using anti-CD16/32 (93, ThermoFisher Scientific). Where >1 brilliant violet or brilliant UV dye was used concurrently ...
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bioRxiv - Immunology 2022Quote: ... followed by Fc-receptor blockade with anti-CD16/CD32 (Thermo Fisher #14-0161-85), and then stained for 30 min on ice with the following antibodies in flow cytometry staining buffer (FACS) ...
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bioRxiv - Cell Biology 2023Quote: ... Rabbit anti-phospho ryanodine receptor 2 (Ser2808) antibody (#PA5-104444) is from Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... Fc receptors were blocked with anti-CD16/CD32 (diluted 1:50, Thermo Fisher Scientific) and cells were stained with cell surface antigens ...
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bioRxiv - Systems Biology 2023Quote: ... ryanodine receptor (RyR2; mouse monoclonal antibody, Cat. # MA3-916, ThermoFisher, Grand Island, NY, USA), connexin 40 (Cx40 ...
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bioRxiv - Cell Biology 2023Quote: ... After Fc receptor blockage using an antibody for CD16/32 (14-0161-85, Invitrogen), stainings were performed in FACS buffer (1 % BSA ...
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Chimeric Antigen Cytotoxic Receptors for In-Vivo Engineering of Tumor-targeting Natural Killer CellsbioRxiv - Immunology 2023Quote: Huh7 cells were transfected with receptor mRNA using the Lipofectamine™ MessengerMAX™ (Invitrogen) mRNA transfection reagent ...
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bioRxiv - Cell Biology 2023Quote: ... Rabbit anti-phospho ryanodine receptor 2 (Ser2808) antibody (PA5-104444) is from Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... unincorporated ATP [γ-32P] was removed using G-50 spin columns (GE Life Technologies) following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2020Quote: ... incubated with (N-γ-maleimidobutyryl-oxysuccinimide ester) crosslinker (4 mM in ethanol, Thermo Scientific), rinsed with ethanol and dried thoroughly in a sterile biosafety cabinet ...
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bioRxiv - Biochemistry 2022Quote: ... were labeled with [γ-32-P]-ATP using T4 polynucleotide kinase (Thermo Fisher Scientific) and hybridized to the membrane in hybridization buffer (4× Denhardts solution ...
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bioRxiv - Immunology 2023Quote: ... TRAC edited and SFG γ-retrovirally-transduced T cells were maintained in RPMI (ThermoFisher) supplemented with 10% FCS (Sigma-Aldrich) ...