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Citations for Thermo Fisher :
351 - 400 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2021Quote: gRNA expression plasmids were purified using PureLink HiPure Plasmid Midiprep Kit (Invitrogen K210004) following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Plasmid material was isolated using the GeneJet plasmid miniprep kit (Thermo Fisher Scientific), sequence verified (GATC-biotech ...
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bioRxiv - Biochemistry 2020Quote: Plasmid DNA was extracted with the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... repair template plasmids were isolated with the GeneJET Plasmid Miniprep Kit (Thermo Scientific) from the overnight E ...
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bioRxiv - Microbiology 2021Quote: Plasmid DNA was routinely purified using the GeneJet plasmid miniprep kit (Thermo Fisher). PCR was conducted with Phusion HiFi polymerase (Thermo Fisher) ...
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bioRxiv - Neuroscience 2019Quote: ... plasmid was isolated using GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific Inc., USA). DNA sequencing was performed in both directions using universal primers for M13 which flanks the multiple cloning site of the plasmid ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmids were extracted using the PureLinkTM HiPure Plasmid Maxiprep Kit (Thermo Fisher Scientific) with the PureLinkTM HiPure Precipitator module (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... the plasmids were isolated by using GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific) and sent for sequencing to confirm the correct assembly ...
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bioRxiv - Microbiology 2022Quote: ... and the plasmids were isolated using the GeneJET Plasmid Miniprep kit (Thermo Fisher). DNA sequencing was carried out to assess the fidelity of the mutagenesis reaction.
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bioRxiv - Neuroscience 2020Quote: ... Plasmid DNA extractions were performed with GeneJET Plasmid Miniprep Kits (Thermo Fisher Scientific). Sanger sequencing reactions were performed by the Molecular Biology Services Unit at the University of Alberta ...
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bioRxiv - Neuroscience 2021Quote: ... The plasmids were collected and purified using GeneJET Plasmid Maxiprep Kit (Thermo Scientific) and the product was sequenced by GATC Biotech to check the orientation of the insert in the plasmid and the identity of the sequence ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmids were purified using GeneJET Plasmid Miniprep Kit (ThermoFisher Scientific, Waltham, MA, USA) and sequenced in both directions to confirm completeness of coding regions.
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bioRxiv - Genetics 2020Quote: ... Plasmids were purified using the PureLink HiPure Plasmid Midiprep Kit (Thermo Fisher Scientific) or GenElute plasmid mini kit (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmids were purified using GeneJET Plasmid Miniprep Kit (ThermoFisher Scientific, Waltham, MA, USA) and sequenced in both directions to confirm complete coding regions.
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bioRxiv - Bioengineering 2022Quote: Plasmid DNA was routinely isolated using GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific) or QIAprep Spin Miniprep Kit (Qiagen) ...
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bioRxiv - Cancer Biology 2022Quote: Cells were transfected with plasmid DNA (see plasmid section) using Lipofectamine 2000 (Invitrogen) according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... were co-transfected with the final plasmid and the pOG44 plasmid (Thermo Fisher), and stable integrated clones were selected with hygromycin.
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bioRxiv - Evolutionary Biology 2023Quote: ... Plasmids were purified using GeneJET Plasmid Miniprep Kit (ThermoFisher Scientific, Waltham, MA, USA) and sequenced in both directions to confirm complete coding regions ...
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bioRxiv - Cell Biology 2023Quote: ... injection plasmids were miniprepped using PureLink HiPure Plasmid Miniprep Kit (Thermo Fisher Scientific). Plasmids were combined at 100 ng/μl SEC plasmids and 50 ng/μl Cas9 plasmid and spun down (15,000 rpm ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmids were harvested with a HiPure Plasmid Filter Midiprep Kit (Invitrogen, Cat #K210014). The library transfer reaction with LR Clonase II was carried out to move inserts into pDEST17 (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... Colonies were then collected for plasmid isolation (Thermo Scientific GeneJET Plasmid Miniprep Kit). Metagenomic inserts in the resistant hits were sequenced by two complementary sequencing methods ...
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bioRxiv - Physiology 2023Quote: ... isolating plasmid DNA using the GeneJET Plasmid Miniprep Kit (Thermo Scientific, Vilnius, Lithuania), and preparing plasmid standards to a known copy number ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmids were then extracted using the GeneJET Plasmid Mini-prep Kit (Thermo Fisher) and verified via Sanger sequencing.
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bioRxiv - Evolutionary Biology 2023Quote: ... Plasmid DNA from positive clones was isolated (GeneJET Plasmid Miniprep Kit; Thermo Scientific) and Sanger-sequenced (Macrogen) ...
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bioRxiv - Biophysics 2023Quote: ... The plasmid DNA was purified using GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific) and eluted in nuclease-free water ...
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bioRxiv - Immunology 2021Quote: ... IL-2 Human Uncoated ELISA kit and IFNγ Human Uncoated ELISA kit (both from Invitrogen) and DuoSet Human IFN-gamma kit and Ancillary Reagent Kit 2 (R&D Systems ...
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bioRxiv - Immunology 2022Quote: ... rat anti-human (16G6) and rabbit anti-human polyclonal CD49a were all purchased from ThermoFisher. For collagen measurements 10 mg of allograft tissue/sample was analyzed with a Hydroxyproline Assay Kit (Sigma ...
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bioRxiv - Pathology 2022Quote: ... Aβ42 human ultrasensitive ELISA Kit and Tau (phospho) [pT231] human ELISA Kit from Thermo Fisher, and sAPPα and sAPPβ ELISA Kit from Mybiosource ...
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bioRxiv - Genomics 2021Quote: ... surviving human leucocytes in thawed samples were removed using anti-human CD45 DynaBeads (ThermoFisher Scientific). The resulting parasite pellet was washed to remove soluble human DNA (hDNA) ...
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bioRxiv - Genomics 2022Quote: Human total RNA was extracted from THP-1 human cells using TRIzol (ThermoFisher, cat# 15596026) as per manufacturer instructions ...
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bioRxiv - Immunology 2023Quote: ... human kinome panel (Cat # 4475388) and human stem cell openarray panel (Cat # 4475390, ThermoFisher, USA). Briefly ...
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bioRxiv - Cancer Biology 2020Quote: ... V2LHS-196739 (shNCOR2-B)) and one non-silencing control construct were selected from the V2LHS pGIPZ based lentiviral shRNA library (Thermo Fisher Scientific). Viral packaging and cellular infection (RPCCC shRNA Resource ...
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bioRxiv - Cancer Biology 2022Quote: ... One million scramble/control or HIF1a targeting shRNA transduced SKOV3ip1 cells were stained using CellTracker™ Red CMTPX Dye (Thermo Fisher Scientific) and added to each well ...
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bioRxiv - Cancer Biology 2023Quote: ... For MDA-MB-231 with stable shRNA-mediated MCT4 KD (described in (Andersen et al., 2018)) the media was supplemented with 1 µg/ml puromycin (Gibco, #A11138-02). MCF10A cells were cultured in DMEM containing 4.5 g/l D-glucose ...
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bioRxiv - Cell Biology 2023Quote: ... and siRNA (corresponding to the shRNA sequences detailed below) were then introduced to the cells following the manufacture protocol (Thermo Fisher Scientific). The cells were left to incubate for 48 hours.
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bioRxiv - Cancer Biology 2023Quote: The shRNA constructs were ordered from Functional Genomics Unit (University of Helsinki) or designed by BLOCK-iT RNAi Designer (Thermo Fisher Scientific) and inserted into pLKO.1 Vector (Addgene) ...
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bioRxiv - Biochemistry 2024Quote: Lentiviral stable knockdown generation was done by growing HEK293T packaging cells in DMEM high glucose supplemented with 10% FBS and after 24 h were transfected with viral components and shRNA against target genes (or scrambled control) using Lipofectamine 2000 (Invitrogen, 11668–019), grown and concentrated using Lenti-X Concentrator (Clontech Laboratory ...
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bioRxiv - Microbiology 2020Quote: ... The plasmid was purified using PureLink™ HiPure Plasmid Maxiprep Kit (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2020Quote: ... 2.5µg psPAX2 packaging plasmid and 1µg pMD2.G envelope plasmid using Lipofectamine 2000 (Invitrogen). Lentiviral supernatant was collected ...
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bioRxiv - Developmental Biology 2021Quote: ... Plasmids were isolated from bacteria using the HQ PureLink Mini Plasmid Purification Kit (Invitrogen) with an extra wash step ...
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bioRxiv - Microbiology 2019Quote: ... Plasmids from white colonies were purified using a GeneJet Plasmid Miniprep kit (Thermo Scientific) following the manufacturer’s protocols ...
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bioRxiv - Microbiology 2019Quote: ... Plasmids in this study were isolated using GeneJET Plasmid Maxiprep Kit (Thermo Scientific™).
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bioRxiv - Cancer Biology 2020Quote: ... Plasmid was isolated from bacteria using the GeneJet Plasmid Miniprep kit (ThermoFisher; Cat#: K0502) or the GeneJet Plasmid Midiprep kit (ThermoFisher ...
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bioRxiv - Synthetic Biology 2021Quote: All expression plasmids were constructed from pENTR plasmids using Gateway cloning (Thermo Fisher Scientific). DNA fragments were either PCR-amplified with oligonucleotide primers (Sigma-Aldrich and IDT ...
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bioRxiv - Microbiology 2021Quote: ... and a plasmid miniprep was prepared using GeneJET Plasmid Miniprep Kit (Thermo Scientific K0702). Plasmids were sequenced with both T7 promoter and terminator primers by the Eurofins A/S (Hamburg ...
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bioRxiv - Evolutionary Biology 2022Quote: ... plates were scraped into PBS and plasmid isolated (GeneJET Plasmid Miniprep Kit, Thermo Scientific). Primers encoding 8-bp 5’ and 3’ barcodes were used to amplify samples from each condition.
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bioRxiv - Microbiology 2022Quote: ... The plasmid DNA was isolated using the Gene Jet Plasmid Miniprep Kit (Thermo Scientific) and its sequence was validated by Sanger sequencing (GATC-biotech).
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bioRxiv - Bioengineering 2022Quote: ... Plasmid isolation was carried out using the PureLink™ Quick Plasmid Miniprep Kit (Invitrogen).
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bioRxiv - Immunology 2022Quote: ... The plasmid was purified using PureLink™ HiPure Plasmid Maxiprep Kit (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2019Quote: ... This viral plasmid and a plasmid expressing VSVG were mixed with lipofectamine 2000 (ThermoFisher) according to manufacturer’s instructions and were added to the cell culture ...