Labshake search
Citations for Thermo Fisher :
51 - 100 of 10000+ citations for Human TENC1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Physiology 2022Quote: ... HEK293 cells were transfected with human TRPA1 expressing plasmid using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2020Quote: ... Cells were either transduced with lentiviral particles carrying a control shRNA or a specific shRNA against RTP801 or transfected with lipofectamine 2000 (Thermo Fisher Scientific) with pCMS vectors expressing eGFP (donated by Dr ...
-
bioRxiv - Microbiology 2021Quote: ... and sgRNA or shRNA using Lipofectamine 2000 (Thermo Fisher). Lenti-X Concentrator (TaKaRa #631232 ...
-
bioRxiv - Cancer Biology 2021Quote: ... cells were transfected with pLKO.1 puro vector constructs expressing shRNA against mouse Aldh1a1 or Alhd1a3 and non-silencing control shRNA using Lipofectamine 2000 Transfection Reagent (Thermo Scientific, Waltham, MA) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2020Quote: ... cultured hippocampal neurons treated with adenoviruses expressing either βII-spectrin shRNA or scrambled (control) shRNA were lysed on ice using the RIPA buffer (Thermo Fisher Scientific, 89900) complemented with 1x protease and phosphatase inhibitor cocktail (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2023Quote: The human DBT cDNA plasmid was obtained from the Ultimate ORF collection (Thermo Fisher). Synonymous mutations in the DBT cDNA were generated with Q5-site direct mutagenesis (NEB ...
-
bioRxiv - Developmental Biology 2021Quote: ... shRNA was synthesized using the T7 MegaShortScript kit (Invitrogen, AM1354) with an incubation time of 6 hours ...
-
bioRxiv - Cell Biology 2020Quote: ... shRNA/siRNA transfection was carried out with Lipofectamine 2000 (ThermoFisher) according to the manufacturer’s protocol.
-
bioRxiv - Cell Biology 2020Quote: ... The corresponding direct and reverse oligos for every shRNA (Invitrogen; Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2021Quote: ... Non-silencing shCTRL = RHS4743) lentiviral shRNAs were purchased from ThermoFisher Scientific (Thermo ...
-
bioRxiv - Physiology 2022Quote: Human TRPM6 was either transfected in a bicistronic vector pCINeo/IRES-GFP plasmid or in a pcDNA pDEST53 plasmid (ThermoFisher Scientific, Waltham, MA) (26 ...
-
bioRxiv - Immunology 2020Quote: ... and sgRNA or shRNA lentiviral construct using Lipofectamine 2000 (Thermo Fisher). Lenti-X Concentrator (TaKaRa #631232 ...
-
bioRxiv - Neuroscience 2022Quote: ... and scrambled shRNAs were transfected into the cells using Lipofectamine (GIBCO). Seventy-two hours after transfection ...
-
bioRxiv - Neuroscience 2021Quote: ... Lentiviral shRNAs against mouse ptprt were constructed in pLKO.1 (Invitrogen) and transfected with pMD2g and psPAX into HEK293T cells for viral package ...
-
bioRxiv - Microbiology 2020Quote: ... and NCBP1 were selected from the GIPZ Lentiviral shRNA library (ThermoFisher, sh-eIF4E2 ...
-
bioRxiv - Cancer Biology 2020Quote: Lentiviral shRNA constructs were obtained from Open Biosystem (Thermo Fisher Scientific). Targeting sequences were cut from the pGIPZ constructs using MIuI and XhoI sites and cloned into pInducer10 using the same sites ...
-
bioRxiv - Developmental Biology 2022Quote: ... Concentrations of shRNA was measured with a NanoDrop One (Thermo fisher).
-
bioRxiv - Developmental Biology 2023Quote: ... HaCaT SAM and shRNA cells were done using Lipofectamine 2000 (Invitrogen). Briefly ...
-
bioRxiv - Neuroscience 2021Quote: ... The plasmids were transfected into HEK293 human embryonic kidney cells using Lipofectamine2000 (Invitrogen, Carlsbad, CA, USA). The recombinant proteins were purified with anti-FLAG agarose affinity gel (A2220 ...
-
bioRxiv - Immunology 2019Quote: ... Custom synthesized (IDT biotech) human KCNJ15 cDNA was sub-cloned into the PE1A plasmid (Invitrogen, #A10462), followed by gateway transfer of KCNJ15 into the lentiviral vector ...
-
bioRxiv - Neuroscience 2023Quote: ... The resulting plasmid was transfected into the wild-type human iPSCs using Lipofectamine (CMAX00015, ThermoFisher Scientific). Transfected iPSC clones were selected against 5 μg/ml Puromycin (A1113802 ...
-
bioRxiv - Cell Biology 2024Quote: ... the cells were transfected with 1μg/well of human RGS14 expression plasmid using Lipofectamine 3000 (Invitrogen). After 48 hr ...
-
bioRxiv - Cell Biology 2021Quote: Human LAMP2A was synthesized into pUC57 Plasmid with the sequence: (5’-CAAGTTTGTACAAAAAAGCAGGCTCTCGAGCAatgGTGTGCTTCCGCCTCTTCCCGGTTCCGGGCTCAGGGCTCGTTCTGGTCTGCCTAGTCCTGGGAGCTGTGCGGTCTTATGCATTGGAACTTAATTTGACAGATTCAGAAAATGCCACTTGCCTTTATGCAAAATGGCAGATGAATTTCACAGTACGCTATGAAACTACAAATAAAACTTATAAAACTGTAACCATTTCAGACCATGGCACTGTGACATATAATGGAAGCATTTGTGGGGATGATCAGAATGGTCCCAAAATAGCAGTGCAGTTCGGACCTGGCTTTTCCTGGATTGCGAATTTTACCAAGGCAGCATCTACTTATTCAATTGACAGCGTCTCATTTTCCTACAACACTGGTGATAACACAACATTTCCTGATGCTGAAGATAAAGGAATTCTTACTGTTGATGAACTTTTGGCCATCAGAATTCCATTGAATGACCTTTTTAGATGCAATAGTTTATCAACTTTGGAAAAGAATGATGTTGTCCAACACTACTGGGATGTTCTTGTACAAGCTTTTGTCCAAAATGGCACAGTGAGCACAAATGAGTTCCTGTGTGATAAAGACAAAACTTCAACAGTGGCACCCACCATACACACCACTGTGCCATCTCCTACTACAACACCTACTCCAAAGGAAAAACCAGAAGCTGGAACCTATTCAGTTAATAATGGCAATGATACTTGTCTGCTGGCTACCATGGGGCTGCAGCTGAACATCACTCAGGATAAGGTTGCTTCAGTTATTAACATCAACCCCAATACAACTCACTCCACAGGCAGCTGCCGTTCTCACACTGCTCTACTTAGACTCAATAGCAGCACCATTAAGTATCTAGACTTTGTCTTTGCTGTGAAAAATGAAAACCGATTTTATCTGAAGGAAGTGAACATCAGCATGTATTTGGTTAATGGCTCCGTTTTCAGCATTGCAAATAACAATCTCAGCTACTGGGATGCCCCCCTGGGAAGTTCTTATATGTGCAACAAAGAGCAGACTGTTTCAGTGTCTGGAGCATTTCAGATAAATACCTTTGATCTAAGGGTTCAGCCTTTCAATGTGACACAAGGAAAGTATTCTACAGCTCAAGACTGCAGTGCAGATGACGACAACTTCCTTGTGCCCATAGCGGTGGGAGCTGCCTTGGCAGGAGTACTTATTCTAGTGTTGCTGGCTTATTTTATTGGTCTCAAGCACCATCATGCTGGATATGAGCAATTTTAGGGTACCACCCAGCTTTCTTGTACAAAGTGGACGCGT-3’) Human LAMP2A was subcloned into pLenti6 (Thermo Scientific), a Gateway adapted vector using BP/LR Clonase II (Thermo Scientific) ...
-
bioRxiv - Neuroscience 2020Quote: ... Certified scramble shRNA (ACTACCGTTGTTATAGGTG) was also used as a negative control (Invitrogen). All plasmids used in this study were purified by NucleoBond Xtra purification kit (MACHEREY-NAGEL) ...
-
bioRxiv - Cancer Biology 2020Quote: ... Cells with integrated shRNA were selected with 300μg/ml Hygromycin B (Invitrogen). The GFP expressing cells were isolated by FACS sorting (BD FACSAria Fusion).
-
bioRxiv - Molecular Biology 2022Quote: ... Patz1 shRNA sequences were designed using BLOCK-iT RNAi designer tool (Invitrogen). Sense and antisense oligos were annealed and cloned into pENTR/U6 plasmid (Invitrogen) ...
-
bioRxiv - Immunology 2021Quote: ... and the Ido1 shRNA using Lipofectamine 2000 (ThermoFisher Scientific, Cat. No. 11668027). Cells were transfected in virus collection medium (DMEM supplemented with 1% FBS ...
-
bioRxiv - Cancer Biology 2023Quote: ... while pLL3.7-shRNA infected cells were selected with 2μg/ml puromycin (Gibco). The overexpression and knockdown efficiencies were validated with WB and qRT-PCR ...
-
bioRxiv - Cancer Biology 2023Quote: The seed shRNA sequences were designed by BLOCK-iT RNAi Designer (ThermoFisher) and cloned into lentiviral expressing vector pTY-U6-Pgk-Puro ...
-
bioRxiv - Cancer Biology 2021Quote: ... Human embryonic kidney 293 (HEK-293T) cells were stably transfected with the plasmids using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2023Quote: ... VF-tagged human SYK and FCER1G plasmids for mammalian expression were generated using Gateway cloning technology (Invitrogen) as described previously (47) ...
-
bioRxiv - Immunology 2023Quote: Both plasmids encoding for the LC and HC were transfected into Expi293F human cells (Thermo Fisher Scientific) with purified DNA and polyethylenimine (PEI 1:3) ...
-
bioRxiv - Cell Biology 2022Quote: ... RNaseH1 was PCR amplified using human RNase H1 cDNA in pENTR221 plasmid (Ultimate ORF clones, IOH4870, ThermoFisher Scientific) as a template ...
-
bioRxiv - Molecular Biology 2023Quote: The human eGFP-Sun1 full length (eGFP-Sun1) gene was synthesized and cloned into the pcDNA3.1+ plasmid (Invitrogen) using NheI and XhoI restriction sites ...
-
bioRxiv - Microbiology 2023Quote: ... The human KIF1A coding sequence (DNASU Plasmid Repository, #HsCD00829423, NCBI Nucleotide reference NM_001244008.2) was inserted by Gateway recombination (Invitrogen) to make an in-frame KIF1A-EmGFP fusion.
-
bioRxiv - Immunology 2023Quote: ... Plasmids were then transfected into human embryonic kidney cells (HEK293-E, National Research Council, Canada) using 293Fectin (Thermofisher). Six days post-transfection ...
-
bioRxiv - Cancer Biology 2023Quote: ... Human OTUD4 constructs were cloned without tag or with FLAG-tag into the expression plasmid pcDNA3.1 (Life technologies) using the oligos forward (BamHI ...
-
bioRxiv - Microbiology 2020Quote: Individual shRNAs were designed using the BLOC-iTTM RNAi Designer (Thermo Fisher Scientific). The specificity was tested by BLAST (NCBI) ...
-
bioRxiv - Molecular Biology 2021Quote: ... the expression of shRNA was induced by addition of doxycycline (Thermo Fisher Scientific). To this end ...
-
bioRxiv - Molecular Biology 2019Quote: ... These shRNA sequences were generated using an open-access algorithm (RNAi designer, ThermoFisher) and were as follows ...
-
bioRxiv - Biochemistry 2020Quote: ... Adenoviruses expressing these shRNAs were established as per the manufacturer’s instructions (Life Technologies) and as previously reported 50 ...
-
bioRxiv - Neuroscience 2021Quote: ... shTTL2 and control shRNA (packaging vectors pLP1, pLP2, and pLP-VSV-G, Thermofisher) were produced by co-transfection with the psPAX2 and pCMV-VSV-G helper plasmids ...
-
bioRxiv - Cell Biology 2022Quote: K8 expression was silenced in HeLa cells using a stable shRNA (Thermo Scientific) transduced by lentiviral vectors ...
-
bioRxiv - Cancer Biology 2019Quote: ... Cell lines were transfected with shRNA constructs using Lipofectamine 2000 (Invitrogen, Carlsbad, CA) and 48 h post-transfection selected with 4 µg/ml puromycin (Invitrogen) ...
-
bioRxiv - Immunology 2021Quote: ... Lentivirus expressing shRNAs were produced by DNA transfection using Lipofectamine 3000 (Thermo Fisher). Transduction was performed using Polybrene (Millipore ...
-
bioRxiv - Cell Biology 2023Quote: ... Stable shRNA-expressing cell lines were maintained in 2.5 μg/ml puromycin (Gibco) following initial selection at 5 μg/ml.
-
bioRxiv - Neuroscience 2021Quote: Primary hippocampal neurons were transfected with 1.5 μg human α-synuclein-GFP plasmid DNA [48] using lipofectamine-LTX (Thermofisher) on DIV6 and imaged on DIV10 using an inverted ZEISS Z1 Cell Observer fitted with a Colibri2 cool LED 5-channel fast wavelength switching system ...
-
bioRxiv - Microbiology 2020Quote: ... MDCK-CMAH cells were prepared by transfection of the human CMAH gene in a plasmid under the control of the CMV promoter (pcDNA3.1, Invitrogen). Clones with Neu5Gc expression were determined by HPLC analysis as previously described (63) ...
-
bioRxiv - Immunology 2020Quote: A CRISPR plasmid targeting human Hk2 was generated using the GeneArt® CRISPR Nuclease Vector Kit from Thermo Fisher. Caco-2 cells were purchased from DSMZ (ACC-169 ...
-
bioRxiv - Cell Biology 2023Quote: ... C2C12 myotubes and biopsy- derived human primary myotubes were transfected with plasmids using lipofectamine 3000 (Thermofisher, Waltham, MA, USA) following the supplier’s instructions.