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Citations for Thermo Fisher :
551 - 600 of 10000+ citations for Human Procollagen II C Terminal Propeptide PIICP CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: Secreted APOE was measured using human APOE ELISA kit (Thermo Fisher Scientific, EHAPOE) following manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The ELISA was performed using Human IL-1b high sensitivity kit from Invitrogen. The samples for ELISA were media collected after treating the cells with drugs at 100uM ...
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bioRxiv - Developmental Biology 2023Quote: ... and urea secretion were determined with the AFP Human ELISA Kit (Invitrogen, EHAFP) (1:3000 dilution) ...
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bioRxiv - Cell Biology 2024Quote: ... wild-caught mice and humans using PureLinkTM RNA Mini Kit (Thermo Fisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: WBM cells were incubated for 30 minutes at 4°C with antibodies for HSC isolation (CD34, CD48, Sca, c-Kit; Invitrogen), lineage markers (CD3e ...
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bioRxiv - Neuroscience 2022Quote: Total RNA was isolated from the primary fibroblast cultures derived from patient PFS and 4 healthy unrelated gender-matched controls (3 homozygous for wild-type C allele at rs324420 and 1 heterozygous C/A) using the PureLink RNA Micro Kit (Invitrogen) and run by Eurofins on the human Clariom D transcriptomic array (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... 60 minutes at 42°C followed by 5 minutes at 70°C or using the high-capacity cDNA reverse transcription kit (4368814, ThermoFisher) with the following programme ...
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bioRxiv - Biochemistry 2023Quote: ... The protein:RNA mixture was then incubated with anti- c-myc agarose beads from a Pierce c-Myc Tag IP/Co-IP kit (ThermoFisher) for 2.5 hr ...
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bioRxiv - Microbiology 2024Quote: ... and human (goat anti-human IgG, ThermoFisher) were also used ...
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bioRxiv - Immunology 2021Quote: PBMC derived CD4+ T cells were isolated from HIV-infected MACS participants using EasySep™Human CD4+ T Cell Isolation Kit and activated overnight with Human T-Activator CD3/CD28 Dynabeads® (Life Technologies). The next day Dynabeads were separated from the CD4+ T cells by manual dissociation followed by magnet isolation ...
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bioRxiv - Biochemistry 2021Quote: ... CellfectinTM II (ThermoFisher) was employed to transfect 10 μg of bacmid into 1×106 attached Sf9 cells following manufacturer’s protocols (ThermoFisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... SuperScript II (Invitrogen) was added and the reaction continued at 42°C for 15 min ...
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bioRxiv - Cancer Biology 2023Quote: ... dispase II (Gibco), and DNaseI (Roche ...
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bioRxiv - Cancer Biology 2021Quote: Full-length human SETD7 cDNA sequences were amplified from a human mRNA pool generated by RT-PCR using SuperScript II RNase H Reverse Transcriptase (Life Technologies,CA, USA). The SETD7 cDNA was cloned into the EcoRI/BamHI site of a pCDH-CMV-MCS-EF1α -Puro vector (System Biosciences ...
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bioRxiv - Immunology 2021Quote: ... and the ELISPOT plate was incubated for 4 hours at 4°C with biotinylated goat anti-human IgG Fc (Invitrogen), followed by incubation for 1 hour at room temperature with horseradish peroxidase (HRP)–conjugated avidin (Vector Laboratories) ...
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bioRxiv - Genomics 2019Quote: ... 500 ng of in situ Hi-C libraries were first mixed with 2.5 μg human Cot-1 DNA (Invitrogen #15279011), 2.5 μg salmon sperm DNA (Invitrogen #15632011) ...
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bioRxiv - Molecular Biology 2019Quote: Huh-7 (human hepatoma) cells were maintained at 37 °C and 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM, Life Technologies) supplemented with 10% (vol/vol ...
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bioRxiv - Microbiology 2020Quote: ... and human hepatoma Huh7.5 cells83 were maintained at 37°C and 5% CO2 in Dulbecco’s Modified Eagle Medium (Invitrogen, Paisley, UK) supplemented with 10% heat inactivated fetal bovine serum (Sigma ...
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bioRxiv - Cell Biology 2020Quote: Parent CHO-K1 and CHO-K1 cells stably expressing extracellular ACP-tagged human insulin receptors were maintained at 37°C with 5% CO2 in HAM’s F-12 medium (Thermo Fisher) supplemented 10% fetal bovine serum (FBS) ...
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bioRxiv - Molecular Biology 2019Quote: ... were incubated for 15 minutes at 37°C in the presence of Alexa-fluor 555 labelled human transferrin (Thermo Fisher) (25 μg/ml ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... as well as human hepatoma Huh7.5 cells28 were maintained at 37 °C with 5% CO2 in Dulbecco’s Modified Medium (DMEM) (Invitrogen, Paisley, UK) containing 10% heat inactivated fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2021Quote: ... SK-N-FI) and NCI-H23 cells (human NSCLC) were cultivated at 37°C with 5 % CO2 in RPMI 1640 medium (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... cells were transfected with an expression plasmid encoding human ACE2 - C-terminally fused to the GFP - using Lipofectamine 2000 (Invitrogen). Two days post transfection ...
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bioRxiv - Cell Biology 2021Quote: ... falciparum cell lines were cultured in vitro in human erythrocytes (UK Blood and Transfusion Service) at 37°C in RPMI-1640 (Gibco) based media supplemented with 5% (w/v ...
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The MuSK-BMP pathway maintains myofiber size in slow muscle through regulation of Akt-mTOR signalingbioRxiv - Cell Biology 2022Quote: ... Serum was diluted 1:1000 in 1/10 block overnight at 4°C and detected using goat anti-human IgG Alexa Fluor 555 (Invitrogen) diluted 1:1000 and counterstained with AlexaFluor488-conjugated α-bungarotoxin (Thermo Fisher ...
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bioRxiv - Molecular Biology 2023Quote: SF268 (human glioblastoma) cells were cultured at 37 °C with 5% CO2 in RPMI medium supplemented with 10% FBS (Gibco). Vero (African green monkey kidney ...
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bioRxiv - Immunology 2023Quote: ... and incubated at 37°C in media with or without 0.5 ng/ml (1.0 unit/ml) human IFN-γ (ThermoFisher Scientific) at time points ranging from 5 minutes to 24 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... human kidney) were maintained at 37°C and 9% CO2 in a 75% humidified atmosphere in Expi293 Expression Medium (GIBCO). The Plasmid was transiently transfected using Expifectamine 293 (GIBCO ...
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bioRxiv - Developmental Biology 2022Quote: Human HEK293 cells were maintained at 37°C in complete media consisting of DMEM with high glucose GlutaMAX Supplement (Gibco) and 10% Fetal Bovine Serum ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary human peripheral mononuclear blood cells (PBMCs) were cultured at 37 °C in 5% CO2 in RPMI 1640 (ThermoFisher, 61870143) supplemented with 10% FBS ...
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bioRxiv - Immunology 2024Quote: ... Primary antibody incubation was carried out overnight at 4 °C with human-CD68 monoclonal antibody (cat. No. 14-0688-82, Invitrogen) and CD19 Rabbit polyclonal antibody (cat ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA was reverse transcribed to cDNA using the SuperScript™ II Reverse Transcriptase Kit (Invitrogen). qPCR reactions were carried out using the SensiFAST™ SYBR® No-ROX Kit (Bioline ...
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bioRxiv - Immunology 2022Quote: ... and cDNA was synthesized using the Superscript II First-Strand Synthesis kit (Invitrogen, Carlsbad, CA). SYBR Green qPCR analysis was performed using the CFX Connect Real-Time system (Bio-Rad ...
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bioRxiv - Biochemistry 2019Quote: ... All point mutations and deletions were created using the QuickChange II Mutagenesis Kit (Thermo Fisher). All mutations were confirmed by DNA sequencing ...
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bioRxiv - Genetics 2021Quote: ... 1μg of total RNA was reverse transcribed using the SuperScriptTM II Reverse Trancriptase kit (Invitrogen) with oligo (dT ...
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bioRxiv - Biochemistry 2022Quote: ... cDNA was synthesized using 1 μg of RNA using SuperScript II Reverse Transcriptase kit (Invitrogen) following the manufacturer’s directions ...
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bioRxiv - Molecular Biology 2020Quote: ... and the entry library was prepared using CloneMiner™ II cDNA library construction kit (Invitrogen) using Gateway technology ...
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bioRxiv - Evolutionary Biology 2022Quote: ... with oligo(dT) primers and the SuperScript™ II Kit (Thermo Fisher Scientific, Waltham, MA), following the manufacturer’s protocol.
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bioRxiv - Plant Biology 2020Quote: ... then cDNA was synthesized by reverse transcription using a SuperScript II Reverse transcriptase kit (Invitrogen) with anchored-oligo(dT)18 primers ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 μg RNA was retro-transcribed using SuperScript II Reverse Transcriptase kit (Life Technologies – Invitrogen), according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 μg RNA was retro-transcribed using SuperScript II Reverse Transcriptase kit (Life Technologies – Invitrogen), according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Poly(dT) cDNA was prepared with Invitrogen™ Superscript™ II reverse transcriptase kit (Invitrogen), and primer efficiency was measured using a cDNA dilution series ...
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bioRxiv - Molecular Biology 2023Quote: ... Following gel purification (QIAquick Gel Extraction Kit) and cloning into pCR II-Blunt-TOPO (ThermoFisher), it was then subcloned into pRRLSIN.cPPT.SYN.WPRE ...
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bioRxiv - Cell Biology 2023Quote: ... we used a Block-iT Pol II miR RNAi expression vector kit (Thermo Fisher Scientific). The targeting sequence of Tmem63b (5′-CGAGGACAGCCTCATAGAGAA-3′ ...
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bioRxiv - Microbiology 2023Quote: ... RNA was reverse transcribed to cDNA using SuperScript II reverse transcriptase kit (Thermo Fisher Scientific) with mycobacterial genome-directed primers.60 The qPCRs were run in the Corbett Rotor-Gene 6000 (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... cloned into pCR-II TOPO vector using TOPO TA cloning kit (Thermo Fisher Scientific, Waltham) and transferred into pDONR207 vector ...
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bioRxiv - Neuroscience 2024Quote: ... cDNAs were generated using Superscript II Reverse Transcriptase First Strand cDNA Synthesis kit (#18064014, Invitrogen) according to the manufacturer manual ...
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bioRxiv - Neuroscience 2021Quote: ... and modified with a N-terminal signalling peptide from the commercial pDisplay vector (Invitrogen) and a trafficking signal prior to tdTomato or citrine fusion and inserted in the pcDNA3.1+ vector (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... with an N-terminal FLAG- or HA-tag (16) or into pcDNA4/TO (Invitrogen) with an N-terminal FLAG- or EGFP-tag (17) ...
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bioRxiv - Biochemistry 2021Quote: ... the N-terminal domain MLKL154 and MLKL139 were cloned into pcDNA3.1 vector (Life Technologies) as previously described [33] ...