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Citations for Thermo Fisher :
551 - 600 of 10000+ citations for Human NID1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: Human neural stem cells derived from H9 (WA09) human embryonic stem cells were purchased from Thermo Fisher. The cells were cultured in complete StemPro NSC SFM (Thermo Fisher ...
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bioRxiv - Neuroscience 2020Quote: NSChIPS (Human Neural Stem Cells derived from the human induced pluripotent stem (iPS) cell line) (ThermoFisher, #A3890101)
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bioRxiv - Genomics 2019Quote: ... a mixture of 23 normal human tissues (including brain) and First Choice Human Brain reference RNA (Ambion), a pool of normal human brain tissues (labeled B) ...
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bioRxiv - Immunology 2022Quote: ... The human codon-optimized Bcl6-P2A-human codon-optimized Bcl-xL insert was synthesized using GeneArt (ThermoFisher) and cloned into pLZRS-IRES-GFP ...
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bioRxiv - Immunology 2020Quote: ... Fresh primary human T cells were isolated and cultured with anti-human CD3/CD28 beads (Dynabeads, ThermoFisher), 500U/mL IL-2 (Proleukin ...
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bioRxiv - Molecular Biology 2021Quote: Human adipose-derived stem cells (ASCs) and human microvascular endothelial cells (HMVEC) were purchased from Thermo Fisher Scientific (Carlsbad ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Primary human epidermal melanocytes (MC) and primary human epidermal keratinocytes (KC) were obtained from Invitrogen (NY, USA). MC was cultured in Medium
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bioRxiv - Molecular Biology 2022Quote: ... Human TNF-α Ultrasensitive ELISA Kit (Invitrogen,KHC3014 [96 tests] and Human IFNα ELISA Kit [Invitrogen,BMS216TEN]) were used for cytokine quantification.
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bioRxiv - Biochemistry 2023Quote: ... 1.0 mg/mL recombinant human insulin, 0.55 mg/mL human transferrin, 0.67 μg/mL sodium selenite, Gibco), and 0.04 μg/mL dexamethasone (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: ... Human and non-human primate OBs were sectioned following embedding in optimal cutting temperature (O.C.T.) compound (ThermoFisher). One human non-synucleinopathy case was obtained from banner health embedded in paraffin wax (Specimen details see Table 1 and SI Appendix Table S1) ...
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bioRxiv - Immunology 2023Quote: Primary human T cells were pre-activated with DynabeadsTM human T-Activator CD3/CD28 beads (Gibco, #11131D) at a 1:1 ratio of cells:beads with 100 IU/ml IL-2 (Chiron #53905-991-01 ...
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bioRxiv - Molecular Biology 2023Quote: ... T cells were isolated from human PBMCs with an Untouched Human T-cell Isolation Kit (Invitrogen, USA) and activated with Dynabeads Human T Activator CD3/CD28 (Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... Human fibroblasts and Human embryonic kidney (HEK) 293T cells were cultured in DMEM (1g/L glucose) (GIBCO) supplemented with 10% Fetal Calf Serum (FCS) ...
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bioRxiv - Neuroscience 2020Quote: ... because we were not able to identify a specific shRNA target sequence using web-based design tools (InvivoGen siRNA Wizard, and Invitrogen Block-iT RNAi Designer). For calcium imaging ...
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bioRxiv - Biochemistry 2019Quote: NPM1 knockdown cell line was generated in AW13516 cell line by transfecting with NPM1 shRNA construct in pTRIPZ vector (Thermofisher Scientific, Waltham, MA, USA) for a period of 24 h followed by antibiotic selection using 1.6 µg/ml puromycin for 4 days ...
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bioRxiv - Microbiology 2020Quote: ... Maxipreps of rescue plasmids were purified using PureLink™ HiPure Plasmid Maxiprep Kit (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2019Quote: ... Transfection grade plasmid DNA were isolated using the PureLink HiPure Plasmid Miniprep Kit (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2021Quote: ... The plasmid was purified using a maxiprep commercial kit (PureLink HiPure Plasmid Filter Maxiprep Kit, Invitrogen). BV2 cells were seeded in 24 well-plates at 70-90% confluence 24 hours prior to the experiment in high glucose DMEM (Gibco ...
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bioRxiv - Genomics 2019Quote: ... cells were transfected with 40 ng renilla plasmid + 400 ng pGL4.23 plasmid using Lipofectamine 2000 (Invitrogen). 48 hours following transfection ...
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bioRxiv - Plant Biology 2019Quote: ... Cas9 and sgRNA plasmids were extracted with the PureLink HiPure Plasmid Midiprep Kit (Invitrogen, cat#K210005). Single stranded salt-free oligonucleotide templates at the length of 42-62 nt were synthesized and dissolved in sterile water at a final concentration of 50 μM ...
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bioRxiv - Biochemistry 2020Quote: ... and plasmid DNA isolated using a PureLink Fast Low-Endotoxin Midi Plasmid Purification Kit (Thermo Scientific). The purified plasmid was finally digested again with HindIII/SnaBI and gel-purified ...
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bioRxiv - Bioengineering 2021Quote: ... Plasmids were harvested from the cultures using the PureLink™ Quick Plasmid Miniprep Kit (Invitrogen™). After extraction of the assembled plasmids ...
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bioRxiv - Neuroscience 2021Quote: ... and transfected with plasmids (pCAGGS-EGFP and the px333 plasmids) using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmids used for injection were purified using the PureLink HQ Mini Plasmid DNA Purification Kit (ThermoFisher) using the extra wash step and buffer recommended for endA+ strains ...
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bioRxiv - Cell Biology 2021Quote: ... packaging plasmid psPAX2 (3μg) and envelope plasmid pMD2.G (3μg) using Lipofectamine 2000 Transfection Reagent (Invitrogen). Post 18 hr ...
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bioRxiv - Biochemistry 2022Quote: ... Plasmid mutant library were purified from the bacteria colonies using PureLink HiPure Plasmid Midiprep Kit (Invitrogen). All primers in this study were ordered from Integrated DNA Technologies.
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bioRxiv - Genetics 2022Quote: ... Plasmid DNA was extracted from 9 OD600nm units using the ChargeSwitch Plasmid Yeast Mini kit (Invitrogen). Plasmid DNA was adjusted to equal concentrations ...
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bioRxiv - Cell Biology 2019Quote: ... cells were transfected with lentiviral plasmids and packaging plasmids using Lipofectamine 2000 (Thermo Fisher Scientific; 11668019) according to the manufacturer’s instructions in Opti-MEM medium (Gibco ...
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bioRxiv - Molecular Biology 2019Quote: ... This plasmid was individually co-transfected with pCMV-PBase plasmid into mESCs using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2019Quote: ... The plasmids of the positive hits were isolated using the GeneJET Plasmid Miniprep Kit (Thermo Scientific) and transformed afresh to the P ...
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bioRxiv - Bioengineering 2019Quote: ... coli cultures were harvested for their plasmid using the GeneJET plasmid miniprep kit (Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2021Quote: ... Guide plasmid combinations and substoichiometric amounts of dsRed plasmid were cotransfected by Lipofectamine® 2000 (Invitrogen) transfection (375 ng each guide plasmid ...
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bioRxiv - Genomics 2020Quote: The plasmids for dCas9-CTCF and gRNA were extracted using PureLink HiPure Plasmid Midiprep Kit (Invitrogen). For the electroporation ...
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bioRxiv - Immunology 2021Quote: ... packaging plasmid (pCMV-dR8.2) and envelope plasmid (pCMV-VSV-G) using Lipofectamine-LTX reagent (Thermo Fisher Scientific India Pvt ...
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bioRxiv - Immunology 2020Quote: ... The plasmids were isolated from bacterial culture using PureLink(tm) Quick Plasmid Miniprep Kit (Invitrogen, USA) or NucleoSpin® Plasmid EasyPure Miniprep Kit (Macherey-Nagel ...
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bioRxiv - Synthetic Biology 2023Quote: Purification of plasmids was done with the Thermo Scientific GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific). The pCAS vectors with split-G418 selection were adapted from Bean et al ...
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bioRxiv - Neuroscience 2023Quote: ... Each plasmid was amplified and purified using PureLink HiPure Plasmid Filter Maxiprep Kit (Cat# K210016, Thermofisher).
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bioRxiv - Neuroscience 2023Quote: ... and co-transfected with 1ug transposon targeting plasmid and 1ug transposase plasmid using Lipofectamin3000 (ThermoFisher, L3000015) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmid DNA was extracted from ten positive clones using the GeneJET Plasmid Miniprep Kit (Thermo Scientific) and Sanger sequenced (EuroFins Genomics) ...
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bioRxiv - Molecular Biology 2023Quote: ... Supercoiled plasmid DNA (pBAD-HisB) was obtained by the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific) and was added to the 1-2 nM concentration ...
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bioRxiv - Microbiology 2023Quote: ... Plasmid extractions were performed per manufacturer protocol using the GeneJET Plasmid miniprep kit (Thermo Fisher Scientific). All plasmid constructs were confirmed by Sanger sequencing at the Massachusetts General Hospital CCIB DNA Core facility or by Illumina sequence at SeqCenter ...
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bioRxiv - Cell Biology 2020Quote: ... plasmids using Lipofectamine 3000 (ThermoFisher# L3000015) following the standard protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... plasmids using Lipofectamine 2000 (Life Technologies) to produce CRISPR lentivirus 54 ...
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bioRxiv - Cell Biology 2021Quote: ... plasmids after BbsI (Thermo Fisher Scientific) digestion ...
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bioRxiv - Cell Biology 2021Quote: ... The pcDNA5_FRT plasmid purchased from Invitrogen was modified to insert the variable regions of B2 heavy and light chains in human IgG1 constant regions ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmid DNA and Lipofectamine 2000 (Invitrogen) were mixed in OptiMEM (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... expression plasmids using Lipofectamine 2000 (Invitrogen) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... and gp130 mutant plasmids (Thermo Scientific), cells were plated 24h prior to transfection to produce monolayers that were 60% confluent and these were transfected by using Turbo DNAfectin 3000 (cat # G3000 ...
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bioRxiv - Microbiology 2021Quote: Plasmid based on pBAD/His (Invitrogen) or pHT01 (MoBiTech ...
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bioRxiv - Bioengineering 2020Quote: ... Plasmids were diluted in OptiMEM (ThermoFisher) and mixed with the FuGENE HD transfection reagent (Promega ...