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Citations for Thermo Fisher :
351 - 400 of 10000+ citations for Human Immunodeficiency Virus Reverse Transcriptase Protein HIV 1 Clade B IIIB since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and 1 μg of RNA reverse transcribed using SuperScript III reverse transcriptase kit (Thermo Fisher; 18080093) with oligo(dT ...
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bioRxiv - Genetics 2023Quote: ... 1 μg of mRNA was reverse transcribed using SuperScript III reverse transcriptase kit (Thermo Fisher Scientific). Quantitative PCR was performed using the fast SYBR Green master mix (Qiagen ...
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bioRxiv - Developmental Biology 2023Quote: ... Total RNA (1 μg) was reverse transcribed using random primers and Superscript III reverse transcriptase (Invitrogen) and quantified by qPCR on Applied Biosystems qPCR instrument in the presence of SYBR green with the primer sequences listed in Supplementary Table 8 using the ΔCT method ...
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bioRxiv - Neuroscience 2023Quote: ... 1 μg of mRNA was reverse transcribed into cDNA using Superscript II reverse transcriptase kit (Invitrogen). The cDNA samples were used for quantitative PCR in the LightCycler 96 system (Roche ...
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bioRxiv - Systems Biology 2023Quote: ... The RNA-protein complexes were then subjected to 3’ adapter ligation and reverse transcription with SuperScript III Reverse Transcriptase (Invitrogen). The cDNA was purified with Agencourt AMPure XP beads (Beckman Coulter ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant human TNF-α a well established trigger of HIV-1 reactivation was purchased from Gibco. The PKC activator Bryostatin and Sodium Butyrate (NaBu ...
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bioRxiv - Microbiology 2023Quote: ... The first-strand cDNA was obtained using oligo(dT) primers and Moloney murine leukemia virus-reverse transcriptase (MMLV-RT kit; Life Technologies, USA), using 100 ng of purified RNA ...
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bioRxiv - Plant Biology 2023Quote: ... 1.0 μg DNase-treated RNA was reverse transcribed in a reaction volume of 20 μl containing oligo-(dT)19 primer and Moloney murine leukemia virus reverse transcriptase (Thermo Fisher Scientific). RT-qPCR analysis was then performed with 1.0 μl of 5-fold diluted cDNA using a SYBR premixed Ex Taq kit (Takara) ...
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bioRxiv - Molecular Biology 2021Quote: ... after which 1 µl of Maxima H Minus Reverse Transcriptase (Thermo Fisher) was added each sample ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1 μL of 200 U/μL SuperScript III reverse transcriptase (Invitrogen).
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bioRxiv - Genetics 2020Quote: ... RNA (1 µg) was retrotranscribed using Revertaid Reverse Transcriptase (Thermo Scientific EP0441) and the cDNA was diluted 1/10 for qPCRs using MESA BLUE MasterMix (Eurogenentec ...
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bioRxiv - Microbiology 2019Quote: ... and 1 μl of SuperScript IV Reverse Transcriptase (200 U/μl) (Invitrogen). 7 μl of mastermix was added to each sample ...
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bioRxiv - Microbiology 2022Quote: ... and 1 µL of SuperScript IV reverse transcriptase (Thermo Fisher, cat#18090200). Reverse transcription was performed with the following cycle ...
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bioRxiv - Microbiology 2020Quote: ... 1~5 μg RNA were reverse-transcribed using RevertAid transcriptase (Thermo Scientific) and random hexamer ...
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bioRxiv - Immunology 2022Quote: ... 1 µL/sample of 200 U/μL SuperScript IV Reverse Transcriptase (Invitrogen), 1 µL/sample of 100 mM DTT (Invitrogen) ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 we also tested extension reactions with Superscript IV Reverse Transcriptase (Invitrogen) by adding 0.25µL SSIV enzyme ...
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bioRxiv - Genomics 2023Quote: ... 1 µl of SuperScript IV Reverse Transcriptase (Thermo Fisher Scientific, cat# 18090200), and nuclease-free water (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... and 1 µl RevertAid M-MuLV reverse transcriptase (200 U, ThermoFisher Scientific). Reaction mixtures were incubated at 25 °C for 10 min followed by incubation at 42 °C for 1 h ...
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bioRxiv - Microbiology 2020Quote: ... generated with SuperScript III Reverse Transcriptase (Invitrogen), random primers and a green fluorochrome as the indicator dye (qPCR master mix ...
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bioRxiv - Neuroscience 2020Quote: ... and SuperScript III Reverse Transcriptase (Life Technologies) in a 20-μl reaction volume ...
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bioRxiv - Genomics 2020Quote: 25 μL of Reverse Transcriptase (Thermo Fisher),
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bioRxiv - Immunology 2020Quote: ... Superscript II reverse-transcriptase (Thermo Fisher Scientific) was used for cDNA synthesis and SYBR Green (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... Superscript IV Reverse Transcriptase Kit (Life Technologies), RNase OUT (Life Technologies) ...
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bioRxiv - Genetics 2020Quote: ... and SuperScript IV Reverse Transcriptase (ThermoFisher #18090050). The manufacturer’s protocol was followed extending the incubation time to 50 minutes at 50°C followed by 10 min heat inactivation at 80°C ...
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bioRxiv - Neuroscience 2019Quote: ... the SuperScript II Reverse Transcriptase kit (Invitrogen) was used ...
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bioRxiv - Developmental Biology 2019Quote: ... and SuperScript III Reverse Transcriptase (Thermo Fisher). cDNA was diluted 10× with nuclease-free water and 2 μl was used as a template for the qRT-PCR ...
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bioRxiv - Genomics 2020Quote: ... except that SuperScript III reverse transcriptase (Invitrogen) was used to synthesize first-strand cDNA ...
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bioRxiv - Molecular Biology 2019Quote: ... 50 U SuperScript SSIII reverse transcriptase (Invitrogen), 5 U RiboLock RNase Inhibitor (Thermo Scientific) ...
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bioRxiv - Developmental Biology 2019Quote: ... and Superscript II Reverse Transcriptase (Life Technologies). Quantitative RT-PCR (Q-PCR ...
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bioRxiv - Molecular Biology 2019Quote: ... SuperScript™ III Reverse Transcriptase (ThermoFisher #18080093) and random primers (p(dN)6 ...
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bioRxiv - Cell Biology 2019Quote: ... Superscript III reverse transcriptase (ThermoFisher, Cat# 18080093) was used to reverse transcribe 1µg of high quality RNA into cDNA according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... 0.75 μl Superscript III reverse transcriptase (Invitrogen), 0.4 μl random primers (Invitrogen) ...
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bioRxiv - Plant Biology 2019Quote: ... SuperScript III Reverse Transcriptase (Thermo Fisher Scientific) was used for first-strand cDNA synthesis following the manufacturers’ protocols with the addition of 1 µl Oligo(dT)18 primer and 1 µl RiboLock™ Ribonuclease Inhibitor (MBI Fermentas) ...
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bioRxiv - Immunology 2021Quote: ... SuperScript IV RNase H Reverse Transcriptase (Invitrogen) was used to synthesize first-strand cDNA with random primer from 1µg of total RNA at 50 °C for 50 min ...
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bioRxiv - Neuroscience 2021Quote: ... RevertAid Reverse Transcriptase (Thermo Fisher Scientific, EP0441) and PowerUP SYBR green master mix (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... and 10 U/μl reverse transcriptase (Invitrogen) was added ...
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bioRxiv - Cancer Biology 2020Quote: ... superscript III reverse transcriptase (Thermo Fisher, 18080044) and random primers were used in a total volume of 20 µL for cDNA synthesis according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... reverse transcriptase conversion to DNA (Applied Biosystems cDNA Reverse Transcription Kit Cat #4368814) ...
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bioRxiv - Neuroscience 2022Quote: ... SuperScript IV Reverse Transcriptase Kit (ThermoFisher, 18090010) was used with 500 ng total RNA and oligoT primers to create cDNA ...
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bioRxiv - Molecular Biology 2022Quote: ... 1U of SuperScript III reverse transcriptase (Invitrogen). The reaction was performed as follows ...
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bioRxiv - Cell Biology 2022Quote: ... or Superscript IV reverse transcriptase (Invitrogen, 18090010). qRT-PCR was performed using a LightCycler™ 480 (Roche ...
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bioRxiv - Developmental Biology 2022Quote: ... SuperScript IV reverse transcriptase (Thermo Fisher Scientific) was used against 100 ng of total RNA ...
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bioRxiv - Microbiology 2022Quote: ... RevertAid H Minus Reverse Transcriptase (Thermo Scientific) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... and the M-MLV Reverse Transcriptase (Invitrogen) following the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2022Quote: ... and Maxima Reverse Transcriptase (Thermo Fisher Scientific). The cDNAs (1-5 ng RNA equivalents ...
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bioRxiv - Plant Biology 2022Quote: ... and SuperScript™ III Reverse Transcriptase (Thermofisher). Real-time PCR was performed on the Applied Biosystems ViiA 7 using Power SYBR Green PCR Master Mix (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... dNTPs and SuperScript IV reverse transcriptase (Invitrogen). V(D)J sequences were amplified separately in 25 µl nested PCR reactions using 3 µl of cDNA in the 1st round PCR and 1.5 µl PCR product in the 2nd round PCR ...
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bioRxiv - Microbiology 2022Quote: ... using SuperScript™ III reverse transcriptase (Invitrogen) as described previously (Barreales et al. ...
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bioRxiv - Developmental Biology 2020Quote: ... SuperScript IV Reverse Transcriptase (Thermo Fisher Scientific) was used according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... and RevertAid Reverse Transcriptase (Thermo Fisher Scientific). U6 snRNA was used as the internal control for both qPCR ...