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Citations for Thermo Fisher :
251 - 300 of 10000+ citations for Human Immunodeficiency Virus Nef Protein HIV 1 Clade B IIIB since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and 1% B-27 supplement (Gibco, Cat. # 17504044), supplemented with 3µM CHIR99021 and 5ng/ml of BMP4 ...
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bioRxiv - Genomics 2023Quote: ... Amphotericin B (1 ug/ml) (Thermo Fisher Scientific). After five passages of coculture ...
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bioRxiv - Genomics 2023Quote: ... 1× B-27 (Thermo Fisher Scientific, cat# 17504044), 10 µg/ml BDNF (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2023Quote: ... B-27 supplement (1% vol/vol ThermoFisher, 17504044) and Glutamax (0.5% vol/vol ThermoFisher ...
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bioRxiv - Cell Biology 2023Quote: ... B-27 supplement (1×, Thermo Fisher Scientific, 17504044), N-Acetyl-L-Cysteine (1 mM ...
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bioRxiv - Cell Biology 2023Quote: ... B-27 supplement (1×, Thermo Fisher Scientific, 17504044), N-Acetyl-L-Cysteine (1 mM ...
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bioRxiv - Microbiology 2021Quote: PBMCs from HIV-negative donors were CD8-depleted (Invitrogen Cat# 11147D) and activated with 0.5μg/mL purified PHA (Remel Cat# R30852801 ...
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bioRxiv - Immunology 2022Quote: HIV-infected cells were stained with LIVE/DEAD AQUA viability (Invitrogen) for 30 mins at RT ...
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bioRxiv - Microbiology 2023Quote: ... were transfected with each plasmid expressing SPΔC of SARS-CoV-2 Delta variant (1.2 µg DNA) or human influenza A virus M2 (5 µg DNA) using Lipofectamine 3000 (Invitrogen, USA) to produce 293TN-S target cells ...
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bioRxiv - Cell Biology 2022Quote: ... The induced proteins were isolated from bacterial pellets lysed in B-PER Bacterial Protein Extraction Reagent (Thermo Fisher Scientific) containing cOmplete ULTRA Protease Inhibitor Tablets (Sigma Aldrich ...
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bioRxiv - Immunology 2020Quote: ... Cells were stained with an anti-human Granzyme B antibody (10µg/ml, clone GB11, Thermo Scientific) followed by a goat anti-mouse IgG Abberior Star 580 (Abberior Instruments) ...
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bioRxiv - Cell Biology 2022Quote: ... RCH-ACV (and derived) human B cells were grown in RPMI culture medium (GIBCO, Cat#22400089) containing 20%-FBS (10270-106 ...
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bioRxiv - Developmental Biology 2023Quote: ... and anti-FREM2 human antibody with 77% immunogen identity with zebrafish Frem2a/b (Invitrogen PA5-55986) were used ...
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bioRxiv - Physiology 2023Quote: ... anti-human HLA-A,B,C to detect leukocyte derived EVs and Annexin V (ThermoFisher, UK), which binds externalized phosphatidylserine (PS ...
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bioRxiv - Genomics 2020Quote: ... Human PDCD1 or PDL1 cell surface protein staining was analyzed using anti-PD-1 (ThermoFisher clone MIH4) or anti-PD-L1 (ThermoFisher clone MIH1 ...
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bioRxiv - Microbiology 2021Quote: ... and mixed 1:1 with B-PER reagent (Thermo Fisher Scientific). Cells were lysed by three passages through a French press at 138 MPa pressure ...
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bioRxiv - Immunology 2022Quote: ... 1% penicillin-streptomycin and 1/1000 b-mercapto-ethanol (Life Technologies) in 96- well U-bottom plates 24 hours prior to co-culture with the indicated combinations of growth/differentiation factors (10 ng/ml each ...
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bioRxiv - Genomics 2020Quote: ... Plasma HIV-1 RNA was extracted using PureLink® Viral RNA/DNA Mini Kit (Invitrogen, Thermo Fisher Scientific, USA) as per manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... Plasma HIV-1 RNA was extracted using PureLink® Viral RNA/DNA Mini Kit (Invitrogen, Thermo Fisher Scientific, USA) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: HIV-1 RNA from the QVOA supernatants was isolated using PureLink™ Viral RNA/DNA Mini Kit (Invitrogen, #12280050) and RT-PCR was performed using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... An aliquot of 50 ng of purified HIV-1 env DNA was used to clone into the pcDNA 3.1D/V5-His-TOPO vector (Invitrogen) and MAX Efficiency Stlb2 competent cells (Life Technology ...
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bioRxiv - Microbiology 2024Quote: ... and the HIV-1 latently infected CD4+ CEM cells ACH-2 (A3.01 cell integrated HIV-1 proviral DNA) were cultured in complete RPIM640 (Gibco) containing 10% FBS and 1% penicillin/streptomycin at 37°C in an atmosphere of 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... BacMam 2.0 virus (Invitrogen, C10593).
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bioRxiv - Microbiology 2019Quote: ... The purified virus was quantified using the bicinchoninic acid (BCA) protein assay kit (ThermoFisher Scientific, Waltham, MA, USA) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... hygromycin B (0.25 mg mL−1) and G418 (1 mg mL-1, Invitrogen). HeLa cells were cultured at 37°C in a 5% CO2-containing atmosphere ...
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bioRxiv - Bioengineering 2022Quote: ... 0.36 pM Recombinant Human Bone Morphogenetic Protein 4 (BMP4, Gibco), and 0.08 pM Activin A (Fisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: Glial-Derived Neurotrophic Factor (GDNF) Recombinant Human Protein (ThermoFisher, #PHC7044)
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bioRxiv - Neuroscience 2020Quote: Brain-Derived Neurotrophic Factor (BDNF) Recombinant Human Protein (ThermoFisher, #PHC7074)
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bioRxiv - Immunology 2022Quote: ... or IgG1 Fc (human) protein (Thermo Fisher Scientific, Rockford, IL) was added to a final concentration of 10 mg/mL and samples were incubated with gentle mixing for 2 hours at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... and pre-coated vitronectin recombinant human protein (ThermoFisher Scientific #A14700) at 0.5 μg/cm2.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Human paraoxonase1 (PON1) recombinant protein was purchased from Thermo Fisher Scientific Inc ...
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bioRxiv - Systems Biology 2024Quote: ... FGF Basic (aa 10 155) Recombinant Human Protein (Gibco #PHG0021), 1% Penicillin-Streptomycin Solution and 1% L-glutamine ...
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bioRxiv - Neuroscience 2024Quote: ... or human TGFβ1 recombinant protein (10ng/mL)(ThermoFisher Scientific, PHG9204) for 24 hours followed by addition of lipopolysaccharides (LPS ...
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bioRxiv - Cell Biology 2021Quote: ... Induced pluripotent stem cells were derived from human fibroblasts using Sendai virus-based reprogramming kit (CytoTune-iPS Sendai Reprogramming Kit; cat #. A13780-01; Thermo Fisher), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... respectively) and 5 μl of 4X TaqMan Fast Virus 1-step mastermix (ThermoFisher). The qPCR plate was sealed and read in the FAM and HEX channels using a QuantStudio™ 5 Real-Time PCR system (ThermoFisher ...
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bioRxiv - Microbiology 2021Quote: ... using the Taqman Fast Virus 1-stepMaster Mix enzyme (Thermo Fisher, Waltham, MA). The limit of detection of this assay is estimated to be 200 genome equivalents/ml saliva or swab fluid ...
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bioRxiv - Microbiology 2021Quote: ... or Taqman Fast Virus 1-step master mix (Applied Biosystems, Foster City, CA) on a LightCycler 96 or LightCycler 480 instrument (Roche Diagnostics ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and 1X TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific; 4444434) for a total reaction volume of 20 μl in 96-well PCR plates (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2019Quote: ... We used the TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) in a total reaction volume of 10 μl ...
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bioRxiv - Microbiology 2022Quote: ... or Taqman Fast Virus 1-step master mix (Applied Biosystems, Foster City, CA) on a LightCycler 96 or LightCycler 480 instrument (Roche Diagnostics ...
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bioRxiv - Immunology 2022Quote: ... the master mix used was TaqMan Fast Virus 1-Step Master Mix (ThermoFisher). Manufacturer’s protocol was followed to add the appropriate volumes of master mix ...
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bioRxiv - Microbiology 2023Quote: ... MCC virus-positive MKL-1 cells were cultured in 1x RPMI (ThermoFisher Scientific) supplemented similarly as stated above ...
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bioRxiv - Immunology 2022Quote: ... after which cells were permeabilised (0.1% Triton X100) and stained with an antibody recognising dengue virus envelope protein (Invitrogen MA1-27093, for DENV serotypes 1-3), pan flavivirus envelope (Millipore MAB10216 ...
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bioRxiv - Neuroscience 2021Quote: ... Loss of virus was monitored by staining with anti-Sendai virus antibody (Invitrogen, cat #14649482).
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bioRxiv - Microbiology 2020Quote: ... HIV negative donors and depleted of CD8+ T cells using Dynabeads (ThermoFisher). Approximately 30 x 106 cells were placed in 30ml of R10 medium ...
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bioRxiv - Immunology 2020Quote: ... BHLHE41 expression in human B cell subsets was assessed by PrimeFlow RNA assay (Thermo Fisher, Oberhausen, Germany) with high sensitivity Alexa Fluor 647-probe targeting human BHLHE41 normalized on CD8A-probe.
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bioRxiv - Cancer Biology 2021Quote: ... Two sets of primers were used: Megaplex RT Primers Human pool A and B (Thermo Fisher, USA). Together ...
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bioRxiv - Cancer Biology 2021Quote: ... and Lamin B (Invitrogen, 50-554-010, 1:1000). Membranes were incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 1% Gentamicin/Amphotericin B (Thermo Fisher Scientific) and 1x LVES (50x stock ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... supplemented with 1% B-27 supplement (Thermo Fisher Scientific), 1% penicillin/streptomycin ...