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Citations for Thermo Fisher :
601 - 650 of 10000+ citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade AE CM240 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Lentiviral vector CSII-CMV-MCS-IRES2-Bsd harboring a cDNA was transfected with the packaging vectors pCAG-HIV-gp and pCMV-VSV-G-RSV-Rev into X293T cells using Lipofectamine2000 transfection reagent (Life Technologies/Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: KBM7 (a kind gift from Thijn Brummelkamp) and Hap-Lat (latent HIV infected KBM7-derived cell lines) were cultured in IMDM media (ThermoFisher Scientifc) supplemented with 10% FCS and 2% Pen/Strep ...
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bioRxiv - Immunology 2020Quote: 293T cells for HIV pseudovirus production were obtained from the American Type Culture Collection (ATCC, #CRL-3216) and cultured in DMEM (Invitrogen, #10313021) supplemented with ...
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bioRxiv - Immunology 2021Quote: ... was synthesized from 10 µl of HIV RNA transcripts or extracted CA-RNA using Random hexamers and SuperScript IV Reverse transcriptase according to manufacturer protocol (Thermo Fisher). Next ...
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bioRxiv - Immunology 2021Quote: ... the PSG3 plasmid was co-transfected into HEK293T cells along with JRFL or YU2 HIV envelope plasmids using Lipofectamine 2000 transfection reagent (ThermoFisher Scientific) to produce single-round of infection competent pseudo-viruses representing multiple clades of HIV ...
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bioRxiv - Immunology 2023Quote: ... S-expressing cells were mixed at a 1:1 ratio with parental CEM.NKr CCR5+ cells (HIV Reagent Program #4376) and the target cell mixture was labelled with Aqua viability dye (Thermo Fisher Scientific) and eBioScience eFluor670 cell proliferation dye ...
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bioRxiv - Microbiology 2023Quote: HEK293T/17 (ATCC #CRL-11268) and TZM-bl cells (NIH HIV Reagent Program #ARP-8129) were cultured in DMEM complete media (DMEM (Gibco #11965092) supplemented with 10% FBS (Gibco #26140079) ...
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bioRxiv - Immunology 2023Quote: ... HIV reporter pseudoviruses expressing SARS-CoV-2 S glycoprotein and carrying the luciferase gene were produced in Expi293F cells (ThermoFisher Scientific) by co-transfection of the pNL4-3 ...
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bioRxiv - Pathology 2022Quote: ... human CTRP3 (Invitrogen, PA5-115061, rabbit anti-human), α-SMA-Cy3 (Sigma ...
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bioRxiv - Bioengineering 2019Quote: MDA-MB-231 human breast cancer cells were cultured using 1:1 DMEM:F12 medium (Life Technologies) supplemented with 50 µg/ml gentamicin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: Huh7 human hepatocellular carcinoma cells were grown in DMEM/F-12 1:1 medium (Thermo Fisher) supplemented with 10% fetal bovine serum ...
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bioRxiv - Cell Biology 2021Quote: ... Anti-Hec1 (1:1000, human monoclonal) and Anti-phospho-Paxillin (1:100, rabbit polyclonal, pTyr31, Invitrogen). Secondary antibodies diluted in antibody dilution buffer was added along with the conjugated Phalloidin-TRITC (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2021Quote: ... Embryos were stained with mouse-anti-human-ZO-1 1:500 (Thermo Fisher Scientific, 33-9100) or guinea pig-anti-zebrafish-VE-cadherin 1:500 (Paatero ...
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bioRxiv - Biophysics 2023Quote: ... and 5 ng/mL prequalified human recombinant epidermal growth factor 1-53 (EGF 1-53; Gibco). Hippocampal neurons were grown in neuro basal medium ...
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bioRxiv - Immunology 2022Quote: ... and against human CXCL4L1 (rabbit anti-CXCL4L1, PA5-21944, Invitrogen; 1:50). Samples were then prepared for microscopy using Duolink® mounting medium with DAPI ...
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bioRxiv - Genomics 2020Quote: ... human Malat1 (positive control, Type 1 Probe, VA1-11317, Thermo Fisher Scientific), and bacterial DapB (negative control ...
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bioRxiv - Neuroscience 2020Quote: ... monoclonal mouse anti-human α-synuclein (1:1000, 4B12, Thermo Fisher Scientific); the goat anti-ChAT (1:100 ...
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bioRxiv - Neuroscience 2022Quote: ... Human samples were embedded in M-1 embedding matrix (Thermo scientific, USA) and cut into 50 µm to 100 µm thick slices on a sliding freezing microtome (LeicaSM2010R ...
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bioRxiv - Immunology 2022Quote: ... Human Jurkat cells (Clone E6-1) were cultured in RPMI (GIBCO, 11875093) containing 10 % FCS and 1 % penicillin-streptomycin (cRPMI) ...
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bioRxiv - Neuroscience 2021Quote: ... or using goat anti-human IgG Alexa555 (Invitrogen, A-21433, 1:500) for Fc detection ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-human α-synuclein 1:1000 (Thermo Scientific; Rockford, IL, USA); goat anti-luciferase 1:5000 (Abcam ...
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bioRxiv - Microbiology 2020Quote: ... Cells were immunolabeled with rabbit anti-human LC3B (ThermoFisher L10382; 1:1000), 0.05% saponin at room temperature for 1h followed by Alexa FluorTM 647 goat anti-rabbit IgG (ThermoFisher A21245 ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 × 106 primary hepatocytes (HEP10 Pooled Human Cryopreserved Hepatocytes; Thermo Fisher Scientific) were injected into the lower pole of the spleen.
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bioRxiv - Developmental Biology 2020Quote: ... rabbit anti-human ZO-1 (Cat# 40-2200, Invitrogen, Camarillo, CA, USA), rabbit anti-mouse CLDN5 (Cat #34-1600 ...
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bioRxiv - Pathology 2021Quote: ... and mouse monoclonal anti-human VCAM1 (dilution 1:25, clone 1.4C3, Invitrogen) for 1h at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... Libraries were pooled and mixed with human Cot-1 DNA (Life Technologies) and xGen Universal Blockers-TS Mix oligos (Integrated DNA Technologies ...
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bioRxiv - Biophysics 2022Quote: ... monoclonal anti-human lamin B2 (IgG2a, mouse, 1:100) (MA5-17274, Invitrogen), polyclonal anti-human MPO (IgG ...
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bioRxiv - Biochemistry 2022Quote: ... and goat anti-human IgG-AF647 (Thermo Fisher Scientific, A21445; 1:1000).
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bioRxiv - Cell Biology 2023Quote: ... goat anti-human Alexa Fluor 647 (1:500; A-21445, ThermoFisher Scientific), and goat anti-guinea pig Alexa Fluor 647 secondary antibody (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... antibody and goat anti-human Alexa-Fluor 633 (Life Technologies, 1:200). To visualize Aurora kinase C ...
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bioRxiv - Immunology 2023Quote: ... Recombinant human IFN-β (1000 U/ml to 1 U/ml, ThermoFisher) was used as positive controls for IRF activation ...
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bioRxiv - Cancer Biology 2023Quote: ... rabbit anti-human phosphor-CJUN antibody (1:2500, PA5-40193, Invitrogen, USA), rabbit anti-human CJUN antibody (1:2500 ...
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bioRxiv - Immunology 2021Quote: Purified human MYC- or Fc-tagged NKp46 were labeled with Alexa647 using the microscale protein labeling kit (ThermoFisher Scientific). Alexa647-labeled NKp46-Myc (50 nM ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... mice were bled and total IgG (mouse and human) was pulled down using protein A beads (ThermoFisher, Catalog # 20333). An in–house sandwich ELISA was used to measure human IgG (control IgG or MS-Hu6 ...
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Enterohepatic Transcription Factor CREB3L3 Protects Atherosclerosis via SREBP Competitive InhibitionbioRxiv - Physiology 2020Quote: cDNAs encoding human full-length of CREB3L3 (NM_032607.2) and green fluorescent protein were cloned into pENTR4 vectors (Life Technologies). In addition ...
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bioRxiv - Genetics 2020Quote: ... the cells and medium were transferred to fresh vitronectin (VTN)-coated 6-well tissue culture plates (10μl truncated vitronectin recombinant human protein (Invitrogen) + 1ml PBS (5μg/ml VTN ...
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bioRxiv - Microbiology 2023Quote: ... and cytokine concentrations were quantified with the Human Cytokine 16-Plex Protein Standard (Thermo Fisher Scientific, Waltham, MA, USA). For quantification of BPI in human sera ...
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bioRxiv - Microbiology 2022Quote: ... acquired MS/MS spectra were searched against a human uniprot protein database in Proteome Discoverer 3.2 (Thermo Fisher Scientific) using Label free Quantification with Minora feature detection ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were cultured on 6-well plates coated with vitronectin recombinant human protein (A14700, Gibco, concentration 0.5 µg/cm) and maintained in Essential 8 Flex medium (A2858501 ...
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bioRxiv - Bioengineering 2023Quote: ... Protein expression was achieved by transient transfection of FreeStyle human embryonic kidney 293 (HEK293-F) cells (Thermo Fisher Scientific). Cells were grown in non-baffled shake flasks (Corning ...
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bioRxiv - Bioengineering 2023Quote: ... The DNA coding for the protein sequences was synthesized using codon-optimization for human host cells (Thermo Fisher Scientific). If the codon optimization for the lysine-stretches resulted in six or more consecutive adenines in the DNA ...
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bioRxiv - Cancer Biology 2021Quote: ... human (Invitrogen). Cells were cultured in DMEM (Corning Cellgro 10-013-CV ...
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bioRxiv - Microbiology 2021Quote: ... Each 10-μl RT-qPCR mixture consisted of 2.5 µL 4X TaqMan Fast Virus 1-Step Master Mix (Life Technologies, Carlsbad, CA, USA), 400 nM each primer ...
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bioRxiv - Microbiology 2020Quote: The TaqMan real-time RT-PCR assays were performed by using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific, MA, USA). Each 20 μl reaction mix contained 5 μl of 4×Fast Virus 1-Step Master Mix ...
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bioRxiv - Pathology 2023Quote: ... RNA was reverse-transcribed and amplified using the TaqMan Fast Virus 1-Step Master Mix RT-qPCR kit (Life Technologies, Carlsbad, CA, USA) on the LC96 instrument (Roche ...
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bioRxiv - Microbiology 2023Quote: ... in a total volume of 25 µL containing 5 µL of template employing Taqman Fast Virus 1-Step Master Mix (Thermo Fisher Scientific, USA). Oligonucleotide primer and probe sequences and thermocycling conditions are detailed in Table S2 ...
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bioRxiv - Molecular Biology 2023Quote: ... The RNA concentrations of 30 samples were normalized to approximately 15 ng µl-1 and TSV was detected and quantified from 2 µl of sample using a TaqMan™ Fast Virus 1-Step Master Mix (Applied Biosystems™) following the OIE recommended protocol and triplicate standards (mean R2 = 0.93 ± 0.06 ...
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bioRxiv - Bioengineering 2022Quote: ... vRNA levels were determined by quantitative real-time PCR (qRT-PCR) using Applied Biosystems™ TaqMan™ Fast Virus 1-Step Master Mix (Thermofisher #4444432) and a primer-probe combination recognizing a conserved region of gag (GAG5f ...
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bioRxiv - Systems Biology 2023Quote: ... and yield was determined by quantitative real-time PCR using the TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems, Foster City, CA) and TaqMan Gene Expression Assay ...
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bioRxiv - Microbiology 2024Quote: ... A 10 µL RT–qPCR reaction to identify the RNA enteric viruses of interest contained 2.5 µL of fast virus 1-step master mix (4X) (Life Technologies, Carlsbad, CA, USA), 500 nM of each specific primers ...