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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for Human Caveolin 2 CAV2 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Purified T cells were activated for 1 to 3 days with Human T-Activator anti-CD3/CD28 dynabeads at a 1:2 bead-to-cell ratio (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed twice with PBS and 100 μL of Goat anti-human IgG (H+L) Alexa647 secondary antibody (2 μg/mL, Invitrogen) and Aqua viability dye was added for 20 minutes at RT ...
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bioRxiv - Immunology 2022Quote: ... and activated in complete RPMI supplemented with 30 U/mL IL-2 with anti-human CD3/CD28 dynabeads (11131D, ThermoFisher) at a 1:1 cell-to-bead ratio ...
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bioRxiv - Immunology 2023Quote: Cell culture supernatants collected at various time intervals from in vitro stimulated T cells of mice or humans were analyzed for IL-2 by ELISA using kits purchased from ThermoFisher Scientific (mouse IL-2 ELISA kit ...
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bioRxiv - Microbiology 2023Quote: ... The murine macrophage RAW264.7 cell line and Human colon adenocarcinoma Caco-2 cell line was cultured in DMEM (Gibco: 11995065) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2023Quote: ... buffer (1× PBS containing 2% FBS) followed by incubation with Alexa Flour 633 goat anti-human IgG (1: 2000 dilution, Invitrogen). Finally ...
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bioRxiv - Immunology 2023Quote: ASCs (FVS780-CD20-CD3-CD14-CD16-CD19+CD27hiCD38hi) were sort-purified from human peripheral blood mononuclear cells into culture media (10% FCS, 2 mM L-glutamine (Gibco), 100 units/mL Penicillin and 100 ng/mL Streptomycin (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2020Quote: ... Canada and maintained in keratinocyte serum-free media supplemented with 0.05 mg/ml of bovine pituitary extract and 0.02 μg/ml EGF recombinant human protein (Life Technologies, Benicia, CA) in a 37°C ...
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bioRxiv - Neuroscience 2021Quote: The concentrations of protein samples from human brain whole homogenate preparations were measured via the bicinchoninic acid (BCA) assay (Micro BCA protein assay kit, ThermoFisher Scientific). 20 μg of total protein were mixed 1:1 with Laemmli buffer and boiled at 95 °C for 10 minutes ...
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bioRxiv - Immunology 2021Quote: ... using HiTrap Protein A columns (Cytiva) for full length human and hamster mAbs and CaptureSelect CH1-XL MiniChrom columns (ThermoFisher Scientific) for Fab fragments ...
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bioRxiv - Immunology 2021Quote: ... using HiTrap Protein A columns (Cytiva) for full length human and hamster mAbs and CaptureSelect CH1-XL MiniChrom columns (ThermoFisher Scientific) for Fab fragments ...
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bioRxiv - Immunology 2021Quote: ... using HiTrap Protein A columns (Cytiva) for full length human and hamster mAbs and CaptureSelect CH1-XL MiniChrom columns (ThermoFisher Scientific) for Fab fragments ...
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bioRxiv - Molecular Biology 2020Quote: ... # 236-EG), 20 ng/ml recombinant human FGF basic protein (R&D, # 4114-TC),1% penicillin/streptomycin (P/S, Invitrogen, SV30010), 1% sodium pyruvate (Gibco ...
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bioRxiv - Genomics 2022Quote: PBMC culture supernatants were analyzed for secreted proteins with Immune Monitoring 65- Plex Human ProcartaPlex Panel (Thermo Fisher Scientific, Carlsbad, CA) as per manufacturer’s instructions (all proteins assayed shown in Table S5) ...
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bioRxiv - Microbiology 2022Quote: ... Cells were seeded in 96-well-plates and treated with 10μg/ml OM-85 concentrate (OM-Pharma) or 100 ng/mL of human recombinant IFN-β protein (ThermoFisher Scientific). The concentration of 10μg/ml of OM-85 was based on previous data with RSV we recently generated [9] ...
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bioRxiv - Molecular Biology 2022Quote: ... 50 pmol of desthiobiotinylated HOXC13-AS was mixed with magnetic beads and incubated with 200 µg of protein lysate from human adult epidermal keratinocytes (ThermoFisher Scientific). Poly(A)25 RNA ...
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bioRxiv - Biochemistry 2023Quote: ... PIK3CA/PIK3R1 (p110a/p85a) recombinant human protein for use in the experiment shown in Figure 4F was purchased from Thermo Fisher Scientific (catalog number PV4788).
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bioRxiv - Molecular Biology 2024Quote: PBMC culture supernatants were analyzed for secreted proteins using Immune Monitoring 65-Plex Human ProcartaPlex™ Panel (Thermofisher Scientific, Carlsbad, CA), following previously documented procedures.43,44 This is a preconfigured multiplex immunoassay kit that measures 65 cytokines ...
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bioRxiv - Cell Biology 2024Quote: ... human TIM4-Fc protein (FUJIFILM Wako Pure Chemical Corporation) was biotinylated with EZ-Link™ Maleimide-PEG11-Biotin (Thermo Fisher Scientific) and conjugated to Dynabeads™ MyOne™ Streptavidin C1 magnetic beads (65001 ...
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bioRxiv - Bioengineering 2024Quote: ... The cell clumps in Matrigel dome were cultured in the EM supplemented with 25 ng/mL human active BMP7 recombinant protein (Gibco; PHC7204) initially for 3 - 6 days to regenerate and form Exp-HLOs using BMP7 ...
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bioRxiv - Cell Biology 2020Quote: ... and VIC labelled human β actin endogenous control probe (Human - 4326315E) or RNA28S5 (Human - Hs03654441_s1) (Thermo Fisher Scientific) so that amplified mRNA can be normalised to β actin or RNA28S5 ...
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bioRxiv - Neuroscience 2021Quote: ... Human recombinant fibroblast growth factor (human FGF2) (10 ng/ml, Invitrogen) was added to Dulbecco’s Modified Eagle’s Medium (DMEM)/F-12 (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... Human T cells were activated with human CD3/CD28 Dynabeads (Invitrogen), at a bead:cell ratio of 2:1 and transduced using supernatant collected from Phoenix-AMPHO cells ...
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bioRxiv - Cancer Biology 2023Quote: ... Antibodies used for human samples were anti-human CD31 (#17031942, Invitrogen), anti-human CD45 (#17945942 ...
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bioRxiv - Cell Biology 2020Quote: ... or anti-goat RFC1 antibody (2 μg/sample) coupled to Protein G agarose beads (Thermo Fisher Scientific) for 2 h on an end-over-end rotator at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... proteins were transferred to the PVDF membrane by using iBlot™ 2 Transfer Stacks (Thermo Fisher Scientific) and iBlot™ 2 Gel Transfer Device (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... and the protein concentration was adjusted to 2 mg/ml using the Bradford method (Thermo Scientific, USA). Samples were then reduced using DTT (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... proteins in the supernatant were reduced with 25 mM tris(2-carboxyethyl)phosphine (TCEP, Thermo Scientific, #20490) for 1 hour at RT ...
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bioRxiv - Synthetic Biology 2019Quote: ... The proteins were transferred from the gel to a nitrocellulose membrane using a iBlot 2 device (ThermoFisher) and the membrane was immunostained using an iBind instrument (ThermoFisher) ...
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bioRxiv - Bioengineering 2021Quote: ... and probed with SARS-CoV/SARS-CoV-2 Spike Protein S2 Monoclonal Antibody (1A9) (ThermoFisher # MA5-35946) and donkey anti-mouse secondary antibodies tagged with an infrared fluorophore (Rockland Immunochemicals) ...
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bioRxiv - Cancer Biology 2022Quote: ... The RNP complex was assembled at 1:2 molar ratio of TrueCut™ Cas9 Protein v2 (Invitrogen) to synthesised gRNA ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were then transferred onto a PVDF membrane using iBlot 2 transfer system (Thermo Fisher Scientific IB24001). Membrane was blocked in 5% Thermo Scientific™ Oxoid™ Skim Milk Powder (Fisher Scientific OXLP0031B ...
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bioRxiv - Microbiology 2019Quote: ... Reacted protein bands were visualized by using Pierce™ ECL 2 Western Blotting Substrate (Thermo scientific, USA) and imaging.
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bioRxiv - Molecular Biology 2021Quote: ... Flag tagged proteins were immunoprecipitated from 2-3mg cellular extracts with Pierce Anti-DYKDDDDK Magnetic Agarose (Invitrogen). Beads were washed 5x with 1ml IP lysis buffer end eluted with 50µl 1xLaemmli buffer ...
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bioRxiv - Developmental Biology 2019Quote: ... TEV protease (a gift from Dirk Görlich) and Histone1-Alexa-488 protein (2 mg/ml, Thermo Fisher) were injected to the desired embryos using Microinjector FemtoJet® (Eppendorf ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 1% antibiotic-antimycotic and 1% protein-free hybridoma medium II (PFHM 2) (Thermo Fisher Scientific) and the respective growth factors and small molecules (8 µM GSK-3 inhibitor CHIR99021 (R&D Systems) ...
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bioRxiv - Immunology 2021Quote: SARS-CoV-2 S protein-expressing plasmids were transfected into HEK293T cells using Lipofectamine 3000 (Invitrogen, USA). 24 h after transfection ...
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bioRxiv - Microbiology 2021Quote: ... The purified protein was finally buffered with PBS and quantified using Qubit 2 Fluorometer (Thermo Fisher Scientific), and stored at −80 °C until use.
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bioRxiv - Immunology 2021Quote: ... or B.1.351 Spike protein (Table 2) were produced using HEK293T cells transfected with Lipofectamine 3000 (ThermoFisher) using IgE-SARS-CoV-2 S plasmid variants (Genscript ...
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bioRxiv - Cell Biology 2022Quote: ... was followed by protein transference to PVDF membranes using an iBlot 2 transfer system (Thermo Fisher Scientific). Membranes were incubated for 45 min in Rockland blocking buffer (Rockland ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins were reduced and alkylated by adding tris(2-carboxyethyl)phosphine (TCEP) (77720, Thermo Scientific, United States) and iodoacetamide (IAA ...
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bioRxiv - Biochemistry 2023Quote: ... and rabbit anti-SARS-CoV-2 spike protein (RBD) polyclonal antibody (Thermo Fisher Scientific; catalog # PA5-114451).
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bioRxiv - Systems Biology 2023Quote: ... Proteins were transferred to PVDF membranes using iBlot 2 dry blotting system (Thermo Fisher Scientific Cat# IB21001). Primary antibodies were used at concentrations recommended by the vendor (see Supplementary Table 3 for antibody information) ...
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bioRxiv - Cell Biology 2023Quote: ... followed by 2 hour incubation at 4℃ with 50 µL of magnetic Dynabeads Protein G (Invitrogen, #10004D). Beads were washed with 1x TBS Tween-20 washing buffer (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... #2 or IgG (rabbit; self-made) were coupled per µl magnetic Protein G dynabeads (Life Technologies 10004D) for 1 h at RT ...
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bioRxiv - Neuroscience 2023Quote: ... primary antibodies for mouse anti-MAP2 (microtubule-associated protein 2; Invitrogen, Catalog No. 13-1500, 1:1000) and guinea pig anti-synapsin1/2 (Synaptic Systems ...
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bioRxiv - Systems Biology 2023Quote: ... proteins were transferred onto an iBlot membrane using an iBlot 2 Western Blot Transfer System (Thermo Fisher). Blots were incubated in skim milk solution (50 g/L skim milk powder in TBS-T ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were then transferred to a nitrocellulose membrane using the Invitrogen iBlot 2 transfer device (ThermoFisher: IB21001), blocked for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were transferred onto nitrocellulose membranes using an iBlot™ 2 Dry Blotting System (Thermo Fisher Scientific). Total protein profile on the membranes was stained using Revert™ 700 Total Protein Stain following instruction (LI-COR ...
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bioRxiv - Genomics 2020Quote: ... SEMA3C protein levels were checked by western blot as described above using rabbit anti-human SEMA3C antibody (1:1000, Thermo Fisher Scientific). Beta-actin (housekeeping gene ...