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Citations for Thermo Fisher :
201 - 250 of 10000+ citations for Human CLEC4M L SIGN His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2021Quote: ... His-tag recombinant SARS-CoV-2 Spike Protein S1/S2 (S-ECD) (1 ng/ml; ThermoFisher Scientific; aa11-1208; RP-87680) or Flag-tag recombinant SARS-CoV-2 Spike RBD (1 ng/ml ...
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bioRxiv - Immunology 2021Quote: ... Two initial rounds of magnetic-activated cell sorting were performed on the library using decreasing amounts of Ray Biotech RBD-coated His-Tag Dynabeads (Thermo Fisher). The resulting enriched library pool was subsequently sorted using a Sony SH800 Cell Sorter ...
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bioRxiv - Cancer Biology 2022Quote: ... p41Plk3 mammalian expression vector for protein expression and purification was generated by performing an LR recombination reaction between the pENTR/D-TOPO entry clone and a Gateway destination vector pcDNA-DEST40 containing His-tag (Life Technologies). ShPlk3s (shRNA-A ...
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bioRxiv - Biochemistry 2022Quote: ... and Pcdh15 EC 1-2 with 6x His and GFP tag at the C-terminus were transfected and expressed in the ExpiCHO suspension cell system (A29129 ThermoFisher Scientific) using the standard prescribed protocol ...
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bioRxiv - Microbiology 2023Quote: ... All collected fractions (3 ml) were analyzed using SDS-PAGE and Western blotting with an anti-His-tag antibody (ThermoFisher Scientific).
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Changes in cell morphology and function induced by NRAS Q61R mutation in lymphatic endothelial cellsbioRxiv - Cell Biology 2023Quote: ... NM_002524.5) with a 3’ terminal 6 × His-tag was chemically synthesized and cloned into the pFastBac1 vector (Invitrogen, Carlsbad, California, USA). cDNA of NRASQ61R was cloned into the pLVSIN-EF1α-AcGFP-C1 vector (Takara Bio Inc. ...
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bioRxiv - Cell Biology 2023Quote: Copper-inducible pGL1 fly expression vectors for N-terminal FLAG- and HA-tagged cDNAs were generated by using EcoRI and XhoI restriction sites to insert the tag and SalI/NotI restriction sites from pRK5-FLAG or pRK5-HA vectors into a pMT-V5-His backbone (Life Technologies), followed by mutation of 2070C>A to remove a SalI site in the backbone ...
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bioRxiv - Cell Biology 2023Quote: ... was added with His-tag and inserted into pcDNA3.4 TOPO vector by pcDNA3.4 TOPO TA cloning kit (Thermo Fisher Scientific, MA) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... and the His-tag was cut off overnight at 4 °C by the addition of 10 U AcTEV Protease (Thermo Fisher). ErCry4a was further purified using anion-exchange chromatography as previously described44 ...
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bioRxiv - Biochemistry 2023Quote: ... coli Methionine Sulfoxide Reductase A and B containing N-terminal His tags downstream of a T7 promoter were synthesized (Invitrogen GeneArt) and transformed into BL21(DE3 ...
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bioRxiv - Immunology 2019Quote: ... HRP-conjugated goat-anti human IgG (H+L) antibody (Invitrogen) prepared in 0.1% blocking buffer was used for detection of peptide-bound antibodies ...
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bioRxiv - Biophysics 2019Quote: ... the MerMAID1 gene was subcloned with a C-terminal TEV protease restriction site and a 6× His-Tag into the pPiCZ vector (Invitrogen, Carlsbad, CA). Zeocin™-resistant positive clones were selected from electroporation-transformed yeast cells ...
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bioRxiv - Cell Biology 2020Quote: S1R was expressed with an N-terminal 6*His-tag fusion in Sf9 cells using Bac-to-Bac baculoviral expression system (Thermo Fisher Scientific) according to manufacturer’s recommendations ...
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bioRxiv - Biophysics 2022Quote: ... followed by an 8× His tag and HRV 3C protease cleavage site was cloned into the modified pFastBac1 (Invitrogen, Carlsbad, CA, USA) vector ...
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bioRxiv - Biochemistry 2021Quote: ... through G-25 resin the peptide-mRNA:cDNA libraries were incubated with cross-linked linked SARS-CoV-2 Mpro (200 nM) immobilized on Dynabeads™ His-tag Isolation & Pulldown (Life Technologies) at 4 °C for 30 min after which the beads were washed with ice-cold selection buffer (3 x 100 μL) ...
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bioRxiv - Cancer Biology 2020Quote: ... with C-terminal twin-Strep-10x His dual tag followed by a stop codon was synthesized using GeneArt Gene Synthesis service (Invitrogen, Carlsbad, CA) with SgfI and MluI restriction sites at the beginning and the end of the ORF sequence ...
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bioRxiv - Immunology 2021Quote: ... an HRV3C protease cleavage site, a TwinStrepTag and Hexa-His-tag (McLellan et al., 2020) was transiently expressed in FreeStyle293F cells (Thermo Fisher scientific) using polyethylenimine (PEI ...
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bioRxiv - Immunology 2020Quote: ... were coated overnight at 4°C with 2 ug/ml of mouse anti-His-tag antibody (Invitrogen cat. #MA1-21315-1MG, Thermo Fisher Scientific) in PBS ...
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bioRxiv - Immunology 2020Quote: ... or with two administrations two weeks apart of 2.5μg Spike protein (2019-nCoV Spike Protein-S1+S2 ECD, His tag, SinoBiological) formulated in final 20mg/ml Imject™ Alum Adjuvant (Thermo Scientific) via bilateral intramuscular injections in the quadriceps (50μl per side) ...
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bioRxiv - Immunology 2021Quote: ... The RBD-6xHIS protein was purified by affinity chromatography of His Tag residues for metals immobilized on the AktaPure system with HisPur ™ Ni-NTA Chromatography Cartridges (Thermo Fisher) column according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... The ScPif1-N-His protein was labeled by binding to a 6x-His Tag Monoclonal Antibody (500 nM) conjugated to a DyLight™ 550 Fluorophore (Cat. # MA1-21315-D550, ThermoFisher Scientific).
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bioRxiv - Immunology 2022Quote: ... and cloned Pst I/Not I in a pPPI4 expression vector containing a hexahistidine (his) tag with Gibson Assembly (Thermo Fisher Scientific). All S constructs were verified by Sanger sequencing and the protein was subsequently produced in human embryonic kidney (HEK ...
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bioRxiv - Biochemistry 2023Quote: ... of randomized RNA library produced for IVM-seq experiment was incubated with equal molarity of 6x His-tag fused target protein complex immobilized on 7 μL Ni-NTA magnetic agarose beads (Thermo Scientific 78605) in the SELEX binding buffer (10 mM Tris pH 8.0 ...
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bioRxiv - Cell Biology 2024Quote: ... Expression of the recombinant LZTR1 proteins was confirmed by Western blot analysis using an anti-His tag monoclonal rabbit antibody (Thermo Fisher Scientific). Following confirmation of expression ...
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bioRxiv - Cell Biology 2024Quote: ... The precipitated proteins were visualized by SDS-PAGE and Western blot analysis using an anti-His tag monoclonal rabbit antibody (Thermo Fisher Scientific) to determine the protein distribution in each fraction.
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bioRxiv - Neuroscience 2023Quote: ... Human fibroblasts and Human embryonic kidney (HEK) 293T cells were cultured in DMEM (1g/L glucose) (GIBCO) supplemented with 10% Fetal Calf Serum (FCS) ...
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bioRxiv - Immunology 2019Quote: ... The full length and truncation sequences were transferred into a Gateway compatible destination vector that includes an N terminal triple-epitope tag (S protein tag, FLAG epitope tag and Streptavidin-binding peptide tag) using the LR clonase enzyme kit (ThermoFisher). Plasmids were transfected into 293T cells using FuGENE transfection reagent (Promega ...
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bioRxiv - Microbiology 2020Quote: ... AlexaFluor-647-conjugated goat anti-human IgG (H+L) Abs (Invitrogen) were used as secondary antibodies ...
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bioRxiv - Immunology 2019Quote: ... HRP-conjugated goat anti-human IgM or IgG (H+L) (Invitrogen) or mouse anti-human IgG1 ...
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bioRxiv - Microbiology 2020Quote: ... AlexaFluor-647-conjugated goat anti-human IgG (H+L) Ab (Invitrogen) and AlexaFluor-647-conjugated donkey anti-goat IgG (H+L ...
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bioRxiv - Cell Biology 2019Quote: ... and goat anti-human IgG (H+L) antibody (Pierce/Thermo Scientific) were added at a dilution of 1:5000 to detect bound rabbit and human antibodies ...
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bioRxiv - Biophysics 2019Quote: ... The purity of affinity purified histidine tagged CsgF was investigated using SDS PAGE and the presence of the histidine tag was confirmed by western blot using an Anti-His (C-Term)-HRP antibody (Life Technologies, Carlsbad, CA).
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bioRxiv - Microbiology 2020Quote: ... and blots were incubated for 1 h with a 1:1,000 dilution of anti 6X-His Tag rabbit antibodies (Thermo Fisher Scientific, Massachusetts, USA) followed by a 1 h incubation with a 1:3,000 dilution of goat anti-rabbit IgG coupled to HRP secondary antibodies (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2022Quote: ... anti-His tag antibody molecules were tagged with the dye Alexa Fluor® 647 NHS Ester (Succinimidyl Ester, ThermoFisher SCIENTIFIC, Catalog number: A20006) using the same procedure as followed in our previous paper.(11 ...
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bioRxiv - Molecular Biology 2022Quote: ... Dm III-2 and Dm III-2 + cys production was confirmed via western blot using 6x His-tag Ab (Invitrogen, Carlsbad, CA, USA). Positive clones were expanded ...
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bioRxiv - Biochemistry 2023Quote: ... The membrane was probed with primary antibodies including anti-tau mouse ET3 (1:1000 dilution) and 6X - His Tag rabbit monoclonal antibody (Invitrogen, 1:3500 dilution). The secondary antibodies used were ...
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bioRxiv - Biochemistry 2020Quote: ... 6x-His-tagged constructs of human and murine SNED1 cloned into pCDNA5/FRT (Thermo Fisher, Waltham, MA) between the FseI and AscI sites were used to transiently transfect 293T cells to validate the anti-SNED1 antibody generated in this study (Supplementary Figure S1) ...
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bioRxiv - Immunology 2020Quote: ... using a secondary antibody recognizing all human immunoglobulin isotypes (goat anti-human IgG H+L-HRP, Invitrogen/ThermoFisher) [12,13] ...
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bioRxiv - Immunology 2020Quote: ... using a secondary antibody recognizing all human immunoglobulin isotypes (goat anti-human IgG H+L-HRP, Invitrogen/ThermoFisher) [12,13] ...
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bioRxiv - Immunology 2023Quote: ... Expression constructs for M35-V5/His and M27-V5/His (both in pcDNA3.1-V5/His, Invitrogen) have been described previously (Munks et al ...
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bioRxiv - Molecular Biology 2022Quote: ... was used against anti-(c-Myc Tag) and goat anti-Rabbit IgG (H+L) Alexa Fluor 750 (Invitrogen) was used against anti-ENG and anti-β-actin ...
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bioRxiv - Immunology 2020Quote: ... Cross-adsorbed goat anti-human IgG (H+L) secondary antibody (A18811, Invitrogen) was used at a dilution of 1:3,000.
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bioRxiv - Cell Biology 2020Quote: ... Alexa Fluor 647 goat anti-human IgG (H+L) (Molecular Probes, A21445) (1:500 IF) ...
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bioRxiv - Microbiology 2021Quote: ... Alexa Fluor-647-conjugated goat anti-human IgG (H+L) Abs (Invitrogen) were used as secondary antibodies to stain cells for 30 min at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and goat anti–human HRP–conjugated IgG (H+L) (Invitrogen, Catalog # A18805) was used to for capture total human IgG ...
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bioRxiv - Microbiology 2021Quote: ... Secondary antibody AlexaFluor™ 488 goat anti-human IgG (H+L) (Invitrogen) was diluted to 1:500 in 1% milk ...
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bioRxiv - Immunology 2021Quote: ... Alexa Fluor-647-conjugated goat anti-human IgG (H+L) Abs (Invitrogen) and goat anti-mouse IgG (H+L ...
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bioRxiv - Microbiology 2022Quote: ... Alexa Fluor-647-conjugated goat anti- human IgG (H + L) Abs (Invitrogen) were used as secondary antibodies to stain cells for 30 min at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Alexa Fluor-647- conjugated goat anti-human IgG (H+L) Abs (Invitrogen) were used as secondary antibodies to detect antibody binding in flow cytometry experiments ...
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... goat anti-human IgG (H+L) Alexa Fluor™ 647 (Thermo Fisher Scientific Cat# A-21445 ...