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Citations for Thermo Fisher :
301 - 350 of 10000+ citations for Human Apelin APLN CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... we labeled the gp120-specific antibody (A32 mAb) with Allophycocyanin (APC) following the manufacturer instructions (Zenon Human IgG labeling kit, Invitrogen) and measured cell binding by flow cytometry ...
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bioRxiv - Physiology 2022Quote: ... human TACAN or their mutants were synthesized by in vitro transcription using the T7 mMESSAGE mMACHINE kit (Invitrogen, Waltham, MA) and injected into oocytes (25 ng each) ...
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bioRxiv - Systems Biology 2022Quote: ... RNA from human skin and wounds was reverse transcribed using TaqMan® Advanced miRNA cDNA Synthesis Kit (Thermo Fisher Scientific). Individual miRNA expression was then quantified using TaqMan® Advanced miRNA Assays (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: Total or individual CD8+ or CD4+ T cells were purified from PBMC suspensions from our PLWH and HIV-1 negative control study cohorts by negative immunomagnetic selection (purity >90%) using the Untouched Human T cell and CD4 T cell Dynabeads Kits (Invitrogen) and the MojoSort Human CD8 T cell (BioLegend ...
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bioRxiv - Cancer Biology 2019Quote: ... and CD19+ B-cells were isolated by negative selection using the Dynabeads® Untouched™ Human B Cells kit (Invitrogen). Mantle cell lines (Z-138 ...
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bioRxiv - Molecular Biology 2019Quote: ... The resulting supernatant was decanted and the concentration of secreted IL6 was measured by the human IL6 ELISA kit (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Genetics 2019Quote: ... Extracted DNA samples were quantified following the Quantifiler Duo Human DNA Quantification Kit (Thermo Fisher Scientific, Inc., Waltham, MA, USA) as recommended by the manufacturer in 7500 Real-time PCR System (Thermo Fisher Scientific Company ...
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bioRxiv - Bioengineering 2021Quote: ... IL-8 concentrations in medium samples from the basal compartment were measured using the IL-8 Human Uncoated ELISA Kit (88-8086-88, Invitrogen). Assays were performed in Nunc MaxiSorp (tm ...
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bioRxiv - Biophysics 2020Quote: Expression was conducted according to the manual of 1-Step Human High-Yield Mini in vitro translation (IVT) kit (Product No. 88891X) distributed by ThermoFisher Scientific (Pierce Biotechnology ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells and NK cells were isolated from cryopreserved PBMCs using commercial kits (MagniSort Human CD4+ T Cell Enrichment; Affymetrix, and MagniSort™ Human NK cell Enrichment ...
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bioRxiv - Immunology 2022Quote: ... Globin mRNA was depleted from a portion of each total RNA sample using the GLOBINclear™-Human kit (Thermo Fisher). Following the removal of globin transcripts transcriptome profiles were generated via mRNA sequencing ...
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bioRxiv - Cell Biology 2022Quote: ... Three technical replicates of 100 µg protein from each biological replicate diluted with diluent supplied by the manufacturer were added to the antibody-coated wells (Ferritin Human ELISA Kit, Invitrogen). All the steps were carried out following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Medium from each condition was removed and E-cadherin concentrations were quantified by using Human E-Cadherin Quantikine ELISA kit (Invitrogen). The Human E-Cadherin EIA kit protocol was followed as described ...
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bioRxiv - Immunology 2019Quote: ... Concentrations of 30 cytokines were measured using the 30-plex magnetic human Luminex cytokine kit (all using lot ID 1805187A, ThermoFisher). Samples were measured on a LX200 (Luminex ...
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bioRxiv - Molecular Biology 2021Quote: ... Human globin mRNA was depleted from all samples except from samples #1 and #2 using the GLOBINclear kit (ThermoFisher Scientific). The quality of the RNA was assessed using the Agilent 6000 Pico kit with the Bioanalyzer 2100 (Agilent ...
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bioRxiv - Immunology 2021Quote: - IFNγ and IL-10 levels were measured in the supernatant of the WBA using IFN gamma and IL-10 Human ELISA Kit (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: The VEGF-A concentration in the culture medium was analyzed using a VEGF-A Human ProcartaPlex Simplex Kit (Cat. no. EPX01A-10277-901, Invitrogen), specific for the detection of VEGF-A165 ...
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bioRxiv - Immunology 2020Quote: Cytokines (IL-5 and IL-13) in supernatant of human and murine ILC2 cell cultures were analyzed with ELISA kits from Invitrogen. For human cytokine IL-4 in the supernatant of human ILC2 cell cultures were measured by ELISA kit from BioLegend ...
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bioRxiv - Immunology 2020Quote: ... the cytokine levels were determined by Luminex xMAP technology using Human Custom ProcartaPlex Assay kit (Thermofisher, Cat PPX-04-MXRWEYU) in accordance with the manufacturer’s recommendations ...
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bioRxiv - Immunology 2021Quote: IL-17a ELISAs were performed using Human IL-17A (homodimer) ELISA Ready-SET-Go!™ Kit (Invitrogen™; eBioscience™), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... and enriched for CD8+ T cells with the MagniSort™ Human CD8+ T cell Enrichment Kit (#8804-6812-74, Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Total RNA was extracted from rat glomeruli tissue and human podocyte samples using the mirVanaTM Isolation Kit (Invitrogen, Carlsbad, CA), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... total cell RNA was extracted from FACS-purified LP or HP cell populations from B16-F10 murine or LM-MEL-28 human melanoma cells using PureLink RNA Mini kits (ThermoFisher), including on-column DNase treatment ...
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bioRxiv - Cell Biology 2022Quote: ... >1.5 kb 5’and 3’ homology arms were amplified from genomic DNA isolated from one 6-cm dish of immortalized human retinal pigment epithelial cells (hTERT-RPE1 cells, ATCC) using a Purelink Genomic DNA Mini Kit (ThermoFisher). The π-element was PCR amplified from plasmid #1 ...
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bioRxiv - Cancer Biology 2022Quote: Recombinant human SHBG (ProSpec, Israel) was directly labeled with Alexa Fluor-555 using a protein conjugation kit (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: Total RNA from isolated HeLa nucleoli was depleted of rRNA by the RiboMinus™ Human/Mouse Transcriptome Isolation Kit (Invitrogen). RNA was converted to double stranded cDNA using random hexamer with the NEBNext Ultra RNA First Strand and NEBNext Ultra RNA 2nd Strand Synthesis Kits (NEB) ...
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bioRxiv - Microbiology 2022Quote: ... PBMCs were isolated by density gradient using Lymphoprep (Axis-Shield) and CD4+ T cells purified by negative selection using the Dynabeads Untouched Human CD4+ T Cell Isolation kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... commercially available kits were purchased and the assays done following the manufacturer’s protocol: IL-1β (Mouse: BMS6002; Human: BMS224INST, Thermo Fisher) and HMGB1 (Mouse ...
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bioRxiv - Cancer Biology 2023Quote: ... The pENTR-hMCAK construct was generated by amplifying the coding sequence of human MCAK by PCR and inserting it into a pENTR entry clone using the pENTRTM/D-TOPO kit (ThermoFisher). The pFB-6HF2hMCAK construct was created from pFB-6HFRET2-DEST vector and pENTR-hMCAK constructs using Gateway® cloning technology (Invitrogen) ...
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bioRxiv - Genomics 2023Quote: ... These mRNAs were generated from cloned human cDNAs inserted into pCS2+ and in vitro transcribed using mMessage mMachine SP6 kit (ThermoFisher) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Transcriptome libraries were generated using automated library preparation with the Ion AmpliSeq Transcriptome Human Gene Expression Kit (Thermo Fisher Scientific). The transcriptome libraries were barcoded ...
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bioRxiv - Neuroscience 2024Quote: The quantification of the total amount of soluble tau in S1 fractions was performed by using an ELISA kit (human Tau, Invitrogen), following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: Total RNA from human hippocampal/frontal cortex samples and cultured cells was extracted with mirVana’s isolation kit (Ambion, TX, USA) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Cell culture supernatants collected at various time intervals from in vitro stimulated T cells of mice or humans were analyzed for IL-2 by ELISA using kits purchased from ThermoFisher Scientific (mouse IL-2 ELISA kit ...
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bioRxiv - Pathology 2024Quote: ... A mouse anti-human CD11b activated labeled with FITC (clone CBRM1/5, eBioscience) and elastin (EnzChek Elastase Assay Kit, ThermoFisher) were used in association with a rat anti-mouse LY6G antibody for the evaluation of neutrophils activation in cytometry ...
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bioRxiv - Cell Biology 2024Quote: ... The process entailed cloning the various human ACTL7B gene variants into the pENTR TOPO vectors using the pENTR d-Topo cloning kit (Invitrogen), following the manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2020Quote: ... using Taqman probes (human RBM10: Assay ID, Hs00275935_m1, human Bcl-xL: Assay ID, Hs00236329_m1, Applied Biosystems, human Bcl-xS ...
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bioRxiv - Biochemistry 2022Quote: ... Human serum (catalog # 4522 from human male AB plasma) and fetal bovine serum were from Gibco, Life Technologies ...
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bioRxiv - Synthetic Biology 2020Quote: ... and 50 μL fresh FACS buffer and 10 μL human IgG (Human IgG Isotype Control, ThermoFisher Scientific #02-7102 ...
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bioRxiv - Immunology 2022Quote: ... All human CD8+ T cells were activated with anti-human CD3/CD28 dynabeads (11131D, Thermo Scientific) at a 1:1 cell-to-bead ratio and cultured in complete RPMI supplemented with 10% FBS ...
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bioRxiv - Immunology 2022Quote: ... 100 ng/mL Human Recombinant IL-4 and 250 ng/mL Human Recombinant GM-CSF (ThermoFisher) for six days at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... Fragments of human CCDC22 and CCDC93 and full-length human DENND10 were ordered as GeneStrings (ThermoFisher) with the gene codon optimized for E ...
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bioRxiv - Cell Biology 2023Quote: ... Purified human neutrophils and T cells were cultured in Human Plasma-Like Medium (HPLM; ThermoFisher Scientific) with 5% heat inactivated FBS supplemented ...
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bioRxiv - Immunology 2023Quote: ... Primary human T cells were thawed and activated with Human T-Expander CD3/CD28 Dynabeads (Gibco) at a 3:1 bead:cell ratio in complete medium (RPMI 1640 supplemented with 10% fetal bovine serum ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 50 µL fresh FACS buffer and 10 µL human IgG (Human IgG Isotype Control, ThermoFisher Scientific #02-7102 ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 50 µL fresh FACS buffer and 10 µL human IgG (Human IgG Isotype Control, ThermoFisher Scientific #02-7102 ...
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bioRxiv - Cell Biology 2023Quote: ... Human Jurkat T cells and human THP-1 monocytes were cultured in RPMI 1640 medium (ThermoFisher), supplemented with 10% FBS ...
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bioRxiv - Immunology 2024Quote: Fresh human BAL cells and frozen human PBMCs were stained with Aqua live/dead dye (Invitrogen) for 20min at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... human melanocyte growth supplement (HMGS, Gibco), gentamicin (Gibco ...
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bioRxiv - Molecular Biology 2020Quote: ... Pool A (human) (Thermo Fisher Scientific) using the TaqMan® microRNA Reverse Transcription Kit (Thermo Fisher Scientific) ...