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Citations for Thermo Fisher :
251 - 300 of 10000+ citations for Herpes Simplex Virus 2 Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... using the TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems). According to the manufacturer’s instruction (QIAamp Viral RNA mini kit (Qiagen)) ...
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bioRxiv - Microbiology 2022Quote: ... using TaqMan Fast Virus 1-Step Master Mix chemistry (Applied Biosystems). SARS-CoV-2 genomic RNA was amplified and detected using forward (5’- CGTGTAGTCTTTAATGGTGTTTCC-3’ ...
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bioRxiv - Microbiology 2021Quote: ... cells and purified virus particles were lysed with RIPA (ThermoFisher Scientific) buffer supplemented with complete protease inhibitor cocktail (Roche ...
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bioRxiv - Molecular Biology 2020Quote: ... TaqMan™ Fast Virus 1-Step Master Mix (4444432, Life Technologies) was used for one-step RT-qPCR and TaqMan Fast Advanced Master Mix (4444556 ...
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bioRxiv - Microbiology 2020Quote: ... Virus stocks were treated with 40 U/mL DNase I (Invitrogen) for 1 hr at 37°C before they were used to infect cells for 2 hr at an MOI of 0.5-2 ...
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bioRxiv - Physiology 2022Quote: ... and 200 U of Moloney murine leukemia virus reverse transcriptase (Invitrogen) in a final reaction volume of 20 μL (Evans-Molina et al. ...
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bioRxiv - Microbiology 2020Quote: ... using TaqMan Fast Virus 1-Step Master Mix chemistry (Applied Biosystems). SARS-CoV-2 N gene RNA was amplified using forward (5’-GACCCCAAAATCAGCGAAAT ...
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bioRxiv - Genetics 2020Quote: ... Cells were then transduced using CytoTune-iPS 2.0 Sendai virus (Invitrogen) as outlined in the supplier’s protocol at an MOI of 5:5:3 (KOS [Klf4 ...
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bioRxiv - Microbiology 2021Quote: Virus replication assays were performed in RPMI 1640 (Gibco 11875-093) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2023Quote: ... 5 μl of TaqMan Fast Virus 1-Step Master Mix (ThermoFisher), and 9 μl of molecular-grade water per reaction ...
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bioRxiv - Immunology 2023Quote: ... TaqMan Fast Virus 1-Step Master Mix (ThermoFisher, Cat No. 5555532) and a commercially prepared RNAse P primer/probe combination (IDT ...
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bioRxiv - Immunology 2023Quote: ... Taqman Fast Virus 1-step kit (Thermo Fisher; catalog number 4444434) with oligos and probe specific to the N gene of SARS-CoV2 was used for SARS-CoV-2 RNA detection according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... virus inoculum was exchanged with serum-free MEM (Gibco, Life Technologies) containing 0.75% carboxymethylcellulose (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... virus inoculum was exchanged with serum-free MEM (Gibco, Life Technologies) containing 0.75% carboxymethylcellulose (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... dNTP and Moloney murine leukemia virus (M-MLV) reverse transcriptase (Invitrogen) were used to synthesize cDNA ...
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bioRxiv - Molecular Biology 2023Quote: Virus particles were fixed in 1 % glutaraldehyde (Cat# 233281000, Thermo Scientific). A 4 µL aliquot of sample was adsorbed onto holey carbon-coated grid (Lacey ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 µg of whole cell lysates or 2 µg of Nuclear Fractions were boiled in loading buffer (NuPAGE LDS Sample Buffer; Thermo Scientific) with reducing agents (NuPAGE Sample Reducing Agent ...
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bioRxiv - Immunology 2021Quote: ... 10 μl of the lysate was mixed with 100 μl of Tropix Galacto Reaction Buffer Diluent and 10 μl of 2 mM FDG (Thermo Fisher) in 50% DMSO ...
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bioRxiv - Cell Biology 2022Quote: ... were added to 1-2 mg of lysates for overnight incubation at 4 °C before the incubation with protein G Dynabeads (Life Technologies) for additional 2 hrs ...
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bioRxiv - Biochemistry 2022Quote: 250 μg of protein from HEK-293T cell lysates were incubated overnight with 2 μg of a 6x-His tag antibody (MA 1-135, Thermo Fisher) in the presence of a low–salt RIPA buffer (20mM Tris-HCl ...
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bioRxiv - Developmental Biology 2020Quote: ... 5 µL lysate was denatured for 2 min at 85 °C in 9.6 µL Novex Tricine SDS Sample Buffer (2X) (Thermo Fisher Scientific) and 1.4 µL DTT (500 mM) ...
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bioRxiv - Microbiology 2022Quote: ... The resulting cell lysate (L) was then supplemented with 2 mM GTP +/- 40 μM Taxol (Life technologies, cat. R0461 and P3456) and incubated at 37°C for 30 min to induce the polymerization of free tubulin into microtubules ...
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bioRxiv - Genomics 2019Quote: ... 5 μL of lysate was denatured for 2 minutes at 85 °C in 9.6 μL Novex Tricine SDS Sample Buffer (2X) (Thermo Fisher Scientific) and 1.4 μL DTT (500 mM) ...
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bioRxiv - Biochemistry 2020Quote: Whole-cell lysates were obtained incubating cell pellets with a Lysis Buffer (2% n-dodecyl β-dmaltoside (DDM) (Thermo Scientific, #89903) plus 1X protease inhibitor cocktail (Roche ...
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bioRxiv - Microbiology 2023Quote: ... Cell lysates were then mixed with 2 x of the NuPAGE™ LDS Sample Buffer 4 x (Novex, Life Technologies, USA) with addition of 10% β-mercapto-ethanol and incubated for 5 min at 98 °C ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were treated with 0.5 U DNase I per 100 uL lysate (Invitrogen, Waltham, MA, USA) for 15 min ...
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bioRxiv - Genomics 2019Quote: ... lysates were treated with RNaseI (Ambion), and subsequently with SUPERaseIn (Ambion) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... lysates were treated with RNaseI (Ambion), and subsequently with SUPERaseIn (Ambion) ...
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bioRxiv - Microbiology 2019Quote: ... and incubated with primary antibody (Rabbit Anti-Group D antigen, Thermo Scientific Singapore) or Guinea Pig Anti-EbpC 45 at 1:500 dilution for 30 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... Antigen-antibody complexes were visualized by applying a streptavidin-biotin complex (Histostain, Invitrogen) for 10 min followed by NovaRED substrate (Vector Laboratories) ...
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bioRxiv - Microbiology 2020Quote: ... Excess antigen was removed by washing three times with Super block (Thermo Scientific) and unbound sites blocked using non-diluted Sea Block ...
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bioRxiv - Pathology 2021Quote: ... After antigen retrieval with mild Proteinase K digestion (50ug/ml Proteinase K (Ambion) for 2min) ...
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bioRxiv - Immunology 2022Quote: ... was coated as antigen (carbonate buffer or DNA-coating reagent from Invitrogen respectively), overnight ...
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bioRxiv - Microbiology 2022Quote: ... Antigen-antibody complexes were selectively purified using Pierce Protein A Agarose (ThermoFisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... The antigen-antibody reaction was probed by TMB ELISA Substrate Solution (Invitrogen eBioscience) and was terminated with 1M sulfuric acid after 15 minutes ...
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bioRxiv - Immunology 2019Quote: ... Antigen was immobilized on clear polystyrene 96- or 384-well MaxiSorp plates (ThermoFisher) by overnight incubation at 4 C at 2 ng/uL diluted in phosphate-buffered saline (PBS ...
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bioRxiv - Immunology 2021Quote: ... The following antigens and adjuvants were resuspended in endotoxin-free PBS (Life Technologies) and used for vaccination studies as indicated in figure legends ...
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bioRxiv - Microbiology 2021Quote: ... antigen-antibody complexes were recovered using Pierce magnetic IgA/IgG beads (ThermoFisher Scientific), resolved by gel electrophoresis ...
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bioRxiv - Cancer Biology 2021Quote: ... Antigen was visualized with DAB-Peroxidase Substrate (Thermo Fisher Scientific, Waltham, MA, USA). Finally ...
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bioRxiv - Immunology 2020Quote: ... Expi293F cells for recombinant antigen and monoclonal antibody production (Thermo Fisher Scientific, A1452) were maintained at 37°C in 8% CO2 in Expi293F expression medium (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Antigens were coated in a 96 wells plates Nunc-immuno Maxisorp (Thermo Fisher) at 1 μg/mL in carbonate buffer at 4°C overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... Antigen retrieval was performed using Citrate Buffer pH 6.0 (005000, Thermo Fisher Scientific) at 110 °C for 15 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... the viral antigen samples were mixed with 4x LDS loading dye (ThermoFisher Scientific) and 1M DTT ...
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bioRxiv - Immunology 2022Quote: ... Peptide lysates were injected on a trapping column (Acclaim PepMap 100 C18, 3 μm, nanoViper, 75 μm x 2 cm, Thermo Fisher Scientific) with 5 μl/min ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were diluted to 2% (w/v) SDS and protein concentrations were determined using the Pierce BCA Protein Assay (Thermo Scientific, #23225). 20 μg of each lysate were subjected to an in-solution tryptic digest using a modified version of the Single-Pot Solid-Phase-enhanced Sample Preparation (SP3 ...
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bioRxiv - Cancer Biology 2022Quote: ... Equal amounts of lysates were incubated with anti-53BP1 for 2 hours at 4°C and subsequently with Protein A magnetic beads (Thermo Fisher Scientific) for 1 hour at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Cell lysates were prepared by scrapping adherent cells in Tris-glycine sodium dodecyl sulphate (SDS) sample buffer (2×) (Novex, Life Technologies, USA) that contained 800 mM dithiothreitol (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... Total RNA was isolated from MG63 lysates (n = 2 per treatment condition) using the PureLink RNA Mini kit (12183018A, Thermo Fisher Scientific) with DNase set (12185010 ...
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bioRxiv - Microbiology 2020Quote: ... N-term B virus mut) using a Neon® Transfection System (Invitrogen) under the recommended electroporation condition (1350 V ...
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bioRxiv - Molecular Biology 2021Quote: ... We used TaqMan® Fast Virus 1-Step Master Mix (ThermoFisher Scientific) to set up 10 μl (half volume ...