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Citations for Thermo Fisher :
351 - 400 of 10000+ citations for Hepatitis E Virus Capsid Protein ORF2 Mouse Fc Tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: Bacterially expressed ZIKV EDIII proteins (C-terminal 6 × His-tag) were conjugated to Ni-NTA Magnetic beads (Thermo Scientific) following manufacturers protocol ...
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bioRxiv - Plant Biology 2022Quote: DNA templates of twin-strep-tag (TST) modified D1 and uS2c proteins were obtained from Invitrogen (GeneArt Gene Synthesis). Therefore ...
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bioRxiv - Immunology 2023Quote: ... Proteins containing a His-tag were purified by affinity chromatography using HisPur Ni-NTA resin (Thermo scientific, Cat#88222). Proteins were then eluted with 250 mM imidazole in 50 mM Tris-HCl and 300 mM NaCl ...
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bioRxiv - Immunology 2024Quote: ... All supernatants were harvested via centrifugation and the proteins purified using CaptureSelect C-tag affinity matrix (Thermo Fisher Scientific) on an ÄKTA Pure FPLC system (Cytiva) ...
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... Pelleted virus was resolved in PBS (Gibco) and stored at −80°C until further usage ...
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bioRxiv - Immunology 2019Quote: ... murine leukemia virus reverse transcriptase (RT; Invitrogen), and random hexamers (Applied Biosystems) ...
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bioRxiv - Neuroscience 2021Quote: ... Fibroblasts were reprogrammed using Sendai virus (Invitrogen) in feeder-free conditions on Matrigel (Corning ...
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bioRxiv - Cell Biology 2023Quote: ... and EmGFP reporter Sendai Virus (A16519, Invitrogen) were part of the Cytotune -iPS 2.0 Sendai Reprogramming kit and EmGFP Sendai Fluorescence Reporter kit ...
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bioRxiv - Immunology 2024Quote: ... in virus growth medium (1X MEM [Gibco 11095080] ...
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bioRxiv - Microbiology 2021Quote: ... Equal amounts of the cell lysate protein and equal volume of the virus pellet protein were resolved by SDS-PAGE (NuPAGE 4-12% Bis-Tris gel; Invitrogen/Thermo Scientific) and transferred onto nitrocellulose membrane by semidry transfer ...
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bioRxiv - Microbiology 2022Quote: ... The levels of RNA corresponding to the N protein-encoding gene of SARS-CoV-2 were measured using the TaqMan Fast Virus 1-step Master Mix (Thermo Scientific). Each 20-μL reaction mixture contained 5.0 μL of 4× TaqMan Fast Virus 1-Step Master Mix ...
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bioRxiv - Microbiology 2019Quote: ... The viral N-protein was labeled using a primary anti-Tula virus antibody(12) and a secondary anti-rabbit FITC conjugate (Invitrogen, USA). The viral Gc protein was stained using a mouse monoclonal antibody (#H1808-60B ...
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bioRxiv - Bioengineering 2023Quote: ... The levels of RNA corresponding to the N protein-encoding gene of SARS-CoV-2 were measured using the TaqMan Fast Virus 1-step Master Mix (Thermo Scientific). Each 20-μL reaction mixture contained 5.0 μL of 4× TaqMan Fast Virus 1-Step Master Mix ...
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bioRxiv - Cancer Biology 2020Quote: ... the fusion proteins were diluted in mouse serum (Invitrogen) to 200 nM and incubated at 37°C for up to 48 h ...
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bioRxiv - Neuroscience 2020Quote: ... or control mouse IgG and protein G Dynabeads (Invitrogen). Immunocomplexes were eluted by denaturation in NuPAGE LDS sample buffer (Invitrogen) ...
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bioRxiv - Developmental Biology 2022Quote: ... donated foetal tissue stored in Hibernate-E medium (Hib-E, Thermo Fisher Scientific A1247601) was collected from local maternity hospital soon after passing ...
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bioRxiv - Bioengineering 2021Quote: ... One of the following goat-anti-mouse secondary antibodies was used: IgG Fc-HRP (1:10,000, Invitrogen A16084), IgG1 heavy chain HRP (1:50,000 ...
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bioRxiv - Cell Biology 2020Quote: ... secondary antibody (AlexaFluor488 donkey anti mouse IgM 1/400, Life Technologies in Buffer A, 0.1% Saponin 2% FCS) was performed ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were pre-incubated with mouse-Fc block and viability marker prepared in calcium/magnesium-free PBS (Gibco), before staining with fluorescently-conjugated antibodies prepared in staining buffer (2mM EDTA ...
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bioRxiv - Cell Biology 2023Quote: ... anti-mouse integrin β3 (armenian hamster monoclonal, 2C9.G3, Thermo Fisher Scientific, 13-0611-81; FC 1:200), anti-mouse integrin α5 (rat monoclonal ...
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bioRxiv - Cell Biology 2023Quote: ... anti-mouse integrin β1 (Armenian hamster monoclonal, Hmb1-1, Thermo Fisher Scientific, 11-0291-82; FC 1:300), anti-mouse integrin β3 (armenian hamster monoclonal ...
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bioRxiv - Bioengineering 2023Quote: ... One of the following goat-anti-mouse secondary antibodies was used: IgG Fc-HRP (1:10,000, Invitrogen A16084), IgG1 heavy chain HRP (1:10,000 ...
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bioRxiv - Immunology 2021Quote: ... Cell pellets were then incubated with a mouse Anti-V5 tag monoclonal antibody (1.2 mg mL−1; Thermofisher Scientific) in PBS 0.1% BSA for 45 min at 4 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... Anti-Myc-tag mouse antibody Magnetic beads were added and immunoprecipitated according to the manufacturer’s protocol (MBL and ThermoFisher). Western blotting was performed according to standard techniques.
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bioRxiv - Microbiology 2024Quote: ... Membranes were then incubated for 1 h at room temperature with the following primary antibodies: mouse anti-His-Tag (dilution 1:1,000, clone HIS.H8, Invitrogen MA121315), mouse anti-Flag (dilution 1:1000 ...
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bioRxiv - Microbiology 2022Quote: ... COS-7 cells grown on 13 mm cover slips were transfected with either the empty pcDNA3.1(+) vector or one expressing the SARS-CoV-2 E protein using Turbofect transfection reagent (ThermoFisher). At 24 h pt ...
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bioRxiv - Immunology 2022Quote: ... E-cadherin (Invitrogen, PA5- 85088), EpCAM (Abcam ...
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bioRxiv - Cancer Biology 2022Quote: ... Cyclin E (Invitrogen, MA5-32358), GAPDH (GeneTex ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... William’s E Medium (Gibco, A1217601) supplemented with Primary Hepatocyte Maintenance Supplements (Gibco ...
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Polarized Mechanosensitive Signaling Domains Protect Arterial Endothelial Cells Against InflammationbioRxiv - Cell Biology 2023Quote: ... E-selectin (Invitrogen #MA1-06506), ZO-1 (Invitrogen #MA3-39100-A488) ...
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bioRxiv - Cell Biology 2023Quote: ... E-cadherin (ThermoFisher, PA5-32178) 1:5000 ...
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bioRxiv - Neuroscience 2021Quote: Cortices were microdissected from both male and female E15.5 mouse embryos and either were plated immediately or stored in Hibernate E (ThermoFisher) and plated within 24 hours ...
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bioRxiv - Microbiology 2020Quote: ... mouse monoclonal anti-e-cadherin at a dilution of 1:100 (33-4000, Thermo Fisher Scientific, Waltham, MA, USA), rabbit polyclonal anti-myeloperoxidase (MPO ...
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bioRxiv - Genomics 2022Quote: ... Mouse cortices were dissected from E17.5/E18.5 mouse embryos and kept up to 24h in 2 mL of Hibernate™-E Medium (ThermoFisher) containing B27 supplement (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... Total RNA was extracted from mouse embryo whole brains/cortices at embryonic day E 13.5-14.5 using Trizol Reagent (Ambion, 15596026) based on Invitrogen protocols ...
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bioRxiv - Microbiology 2024Quote: ... directed against the cytoplasmic domain of E-cad and a monoclonal anti-Occludin mouse mAb (331500, Life Technologies SA) and revealed using an anti-mouse IgG (H+L ...
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bioRxiv - Cancer Biology 2024Quote: ... mouse E-cadherin cDNA (Image Clone #3002385; Open Biosystems, Inc., which was acquired by Thermo Fisher Scientific, Waltham, MA), was cloned into an LXIZ retroviral vector engineered to contain a high affinity 3’-Myc tag [32] ...
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bioRxiv - Microbiology 2020Quote: One milliliter of monomeric capsid (5 mg/ml) was dialyzed in SnakeSkin dialysis tubing (10K MWCO, Thermo Scientific, Waltham, MA, USA) using a buffer that was high in salt and contained a reducing agent (Buffer 1 ...
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bioRxiv - Plant Biology 2020Quote: ... proteins were detected by Coomassie Blue staining or Western blotting using anti-His6-tag antibodies (Thermo Fisher Scientific MA1-21315).
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bioRxiv - Immunology 2021Quote: ... The efficiency of the expression and purification of recombinant proteins were evaluated by 12% SDS-PAGE and Western blotting by using anti-his tag antibodies (1:3,000) (Thermo Scientific) and HRP-conjugated goat anti-mouse IgG (1:10,000 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the protein with an N-terminal His10 tag was expressed in Sf9 cells following the Bac-to-Bac baculovirus system (Invitrogen). Cells were collected and solubilized to extract protein in the buffer containing 25 mM MES pH 6.0 ...
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bioRxiv - Microbiology 2019Quote: ... 1 nM of CAT protein (Chloramphenicol acetyltransferase, containing a 6×His tag at C-terminus) expressed in the same baculovirus-expression system (ThermoFisher) was used to coat plate wells ...
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bioRxiv - Immunology 2022Quote: ... Gamma and Omicron NTD mutants with an N-terminal human IL-2 or Mu phosphatase signal peptide and a C-terminal polyhistidine tag followed by an AviTag were used to express soluble NTD proteins by transiently-transfecting Expi293F cells (GIBCO). After four days ...
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bioRxiv - Microbiology 2020Quote: ... The HA-tagged proteins were captured with the Pierce™ HA-Tag Magnetic IP/Co-IP Kit (Thermo Scientific™). Magnetic beads (0.25 mg ...
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bioRxiv - Cell Biology 2021Quote: ... WT human PRRT2 (gene ID: 112476) was synthesized as N-terminal HA tag fusion protein (Genewiz) in pcDNA3 vector (Thermofisher) and single point mutants were generated on this matrix by standard molecular biology techniques.
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bioRxiv - Microbiology 2019Quote: ... upstream of a Yellow Fluorescence Protein (YFP) encoding gene and an HA tag using the Gateway cloning system (ThermoFisher Scientific). The resulting plasmids were verified by sequencing and mobilized into A ...
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bioRxiv - Molecular Biology 2021Quote: ... truncation and a TEV protease cleavage site followed by a double maltose-binding protein-(MBP) tag at its C terminus was cloned into the pFastBac vector (Invitrogen). To obtain a SV1–Gs complex with good homogeneity and stability ...
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bioRxiv - Immunology 2021Quote: Protein Digestion and Tandem Mass Tag (TMT) labeling: The Pierce detergent compatible Bradford assay kit from Thermo Fisher (Winnipeg, MB) was used to determine the protein concentration ...
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bioRxiv - Microbiology 2024Quote: ... They were produced with a histidine-tag in the Bac-to-Bac™ Baculovirus Expression System (Invitrogen, for VP3 proteins) or Escherichia coli prokaryotic system (for 2xFYVE) ...
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bioRxiv - Bioengineering 2024Quote: ... Antibodies were purified by gravity column using Protein G Sepharose 4 Fast Flow Resin (Cytiva) and proteins containing His tags were purified on Ni-NTA Agarose (R90101 Invitrogen).