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Citations for Thermo Fisher :
251 - 300 of 10000+ citations for HLA A*0201 WT 1 complex Protein Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Primary antibodies against the His-tag (1:2000, Invitrogen) were followed by secondary HRP conjugated antibody (1:2000 ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibodies against the His-tag (1:2000, Invitrogen) were followed by secondary HRP conjugated antibodies (1:2000 ...
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bioRxiv - Biophysics 2019Quote: ... 1 nM LanthaScreen Elite Tb-anti-His Antibody (ThermoFisher), and 400 TRAP220 peptide in a buffer containing 20 mM potassium phosphate (pH 7.4) ...
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bioRxiv - Microbiology 2022Quote: ... anti-His×6 (mouse monoclonal, Invitrogen, 1:10,000-20,000), anti-OmpA (rabbit polyclonal ...
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bioRxiv - Immunology 2024Quote: ... 1 mg of magnetic beads (Dynabeads His-Tag, Invitrogen) were coupled with 200 µg of histidine-tagged SARS-CoV-2 S protein ...
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bioRxiv - Molecular Biology 2020Quote: ... Csm complex concentration was measured according to the Bradford method using the protein assay kit (Thermo Scientific) with bovine serum albumin as the standard.
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bioRxiv - Immunology 2021Quote: ... previously cultured for 24h with IL-15/IL-15R complex recombinant protein (100ng/ml, Thermo Fisher Scientific) and retinoic acid (100ng/ml) ...
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bioRxiv - Neuroscience 2019Quote: ... Affinity purification of the protein-antibody complex was performed using the AminoLink Immobilization Kit (Thermo Scientific #44890). Approximately 6 mg of soluble dNOS exon 16 protein in 2M guanidine in 1X PBS was bound to the agarose beads in the column as antigen ...
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bioRxiv - Molecular Biology 2022Quote: ... The immune-complex was captured using Pierce MS-Compatible Magnetic IP Kit Protein A/G (ThermoFisher 90409) and eluted with 2x laemmli buffer ...
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bioRxiv - Neuroscience 2024Quote: The ribonucleoprotein (RNP) complex consisting of Cas9 protein and sgRNA was assembled using the Trucut V2 (Invitrogen) and Amaxa Human Stell Cell Nucleofector Starter Kit (VPH-5002) ...
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bioRxiv - Cancer Biology 2024Quote: ... The antibody-chromatin complex was then captured with a mix of protein A and G Dynabeads (Invitrogen) for 2 hours at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... recombinant SARS-CoV-2 Spike RBD protein (RBD, His Tag) was conjugated with EZ-LinkTM Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Pathology 2021Quote: ... His-tag recombinant SARS-CoV-2 Spike Protein S1/S2 (S-ECD) (1 ng/ml; ThermoFisher Scientific; aa11-1208; RP-87680) or Flag-tag recombinant SARS-CoV-2 Spike RBD (1 ng/ml ...
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bioRxiv - Biochemistry 2021Quote: ... The His-tagged proteins were purified using HisPur Ni-NTA resin (Thermo Fisher Scientific), at RT ...
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bioRxiv - Immunology 2021Quote: ... and the protein was purified on His-Pur Ni-NTA resin (Thermo Fisher Scientific) followed by size-exclusion chromatography ...
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bioRxiv - Immunology 2020Quote: ... The His-tagged proteins were purified with the HisPur Ni-NTA Resin (Thermo Fisher). To eliminate nonspecific binding proteins ...
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bioRxiv - Biochemistry 2022Quote: ... His-tagged protein was labeled with Alexa Fluor 488 NHS Ester (Thermo Fisher Scientific) per manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2024Quote: ... The His-tagged dAsapPZA protein was expressed and purified following manufacturer guidelines (Invitrogen, USA).
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bioRxiv - Biochemistry 2022Quote: ... combined protein concentrations of complex I and MSP2N2) were determined using the Pierce™ bicinchoninic acid (BCA) protein assay kit (Thermo Fisher Scientific). For the sample subjected to cryo-EM analyses ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 stably expressing the tetracycline (tet)-repressor (Flp-In T-REx HEK293, Invitrogen, Carlsbad, CA, USA) were cultured as above with the addition of 50 μg/mL zeocin and 5 μg/mL blasticidin (Invitrogen ...
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bioRxiv - Biochemistry 2019Quote: HEK293 cells: HEK293 cells (CLS Cat# 300192/p777_HEK293, RRID:CVCL_0045) were cultured in DMEM high glucose (Gibco) and supplemented with 10% FCS (Gibco) ...
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bioRxiv - Molecular Biology 2019Quote: ... WT-1 (c-terminus; 1:100 dilution; PA5-16879; ThermoFisher Scientific, United States), synaptopodin (P-19 ...
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bioRxiv - Developmental Biology 2019Quote: ... BMP2 recombinant human protein (Thermo Fisher Scientific, #PHC7145) was applied for 1h on cells ...
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bioRxiv - Neuroscience 2020Quote: ... Recombinant full-length human G2019S-LRRK2 protein (Invitrogen) was used as a protein standard ...
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bioRxiv - Bioengineering 2022Quote: ... Human BMP-4 Recombinant Protein (Gibco, Catalog # PHC9534). For cell culture of iPSCs on niches ...
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bioRxiv - Cancer Biology 2022Quote: ... IL-8 Monocyte Recombinant Human Protein (ThermoFisher, PHC0884), and Recombinant Human CXCL1/GROα Protein (R&D Systems ...
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bioRxiv - Cell Biology 2022Quote: ... Vitronectin (recombinant human protein) was from Fisher Scientific.
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bioRxiv - Cell Biology 2024Quote: ... 1.61 nM EGF Recombinant Human Protein (Invitrogen, PHG0313), 1.1 μM hydrocortisone (Sigma-Aldrich ...
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bioRxiv - Biophysics 2019Quote: ... The protein was expressed in HEK293S GnTI− cells (ATCC CRL-3022) grown in Expi293 Expression Media (Life Technologies) to densities 2.5 × 106 cells ml−1 ...
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bioRxiv - Molecular Biology 2022Quote: HEK293-6E cells (used for protein expression) were cultured in expression medium (FreeStyle F17 (Gibco, Cat#A13835-01)) supplemented with 1% FBS (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... FreeStyle HEK293 cells (Thermo fisher scientific) were transfected with pcDNA3.1 vector encoding for HSulf-2 cDNA flanked by TEV cleavable SNAP (20.5 kDa ...
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bioRxiv - Cell Biology 2021Quote: Low-passage HEK293 cells (Thermo Fisher) were co-transfected with 10 μg lentiviral vector (pCDH-SYN1-sypHy ...
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A nepenthesin insert allosterically controls catalysis in the malaria parasite protease plasmepsin VbioRxiv - Microbiology 2022Quote: ... we transfected HEK293-F cells (ThermoFisher) using a modified version of the protocol in (41) ...
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bioRxiv - Biochemistry 2021Quote: HEK293 Flp-In T-Rex (Invitrogen), HeLa (Austin et al. ...
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bioRxiv - Biophysics 2021Quote: Suspension-adapted HEK293 Freestyle cells (Invitrogen) in serum free media (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... the Expi-HEK293 cells (Thermo Scientific) were transfected with the mammalian expression plasmid pHLSec containing the recodonized SUB1 ...
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bioRxiv - Molecular Biology 2022Quote: HEK293 TREx cells (Invitrogen Canada Inc.) were cultured in high-glucose Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Microbiology 2023Quote: ... and HEK293-S cells (ThermoFisher Scientific) were cultured in FreeStyle293 expression medium (Life Technologies) ...
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bioRxiv - Immunology 2023Quote: ... FreeStyle™ HEK293-F cells (Gibco) were co-transfected with two plasmids ...
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bioRxiv - Cancer Biology 2022Quote: ... MS/MS were acquired on a Thermo Orbitrap Exploris 480 equipped with (HLA-I) and without (HLA-II) FAIMS (Thermo Fisher Scientific) in data dependent acquisition ...
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bioRxiv - Microbiology 2023Quote: ... MS/MS data were acquired on a Thermo Orbitrap Exploris 480 equipped with (HLA-I) and without (HLA-II) FAIMS (Thermo Fisher Scientific) in data-dependent acquisition ...
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bioRxiv - Cancer Biology 2023Quote: The 387 complex genetics sarcomas were analyzed on Human Genome U133 Plus 2.0 array (900466, Affymetrix, Santa Clara, CA, USA), according to the manufacturer’s procedures ...
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bioRxiv - Cell Biology 2023Quote: The baculovirus preparation for recombinant human HAUS complex expression was carried out according to the manufacturer’s protocol (Bac-to-Bac system, Life Technologies) using E ...
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bioRxiv - Cancer Biology 2019Quote: ... was analyzed using Affymetrix Human Transcriptome Array 2.0 (HTA 2.0) microarrays after cDNA amplification using GeneChip WT Pico Reagent Kit (Affymetrix) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: Human CSF2RB WT (exons 11 to 14) and A455D mutant donor targeting sequences were cloned into pDONR221 (ThermoFisher) with the following layout (Supplementary Figure S1) ...
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bioRxiv - Cancer Biology 2022Quote: Purified anti-HLA-I clone W6/32 (ATCC® HB95) antibodies were cross-linked to protein-A Sepharose 4B conjugate beads (Invitrogen) with dimethyl pimelimidate dihydrochloride (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2019Quote: Protein concentration in g ⋅ L−1 of human serum was calculated using PierceTM Bicinchoninic Acid (BCA) Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... 6x-His-tagged constructs of human and murine SNED1 cloned into pCDNA5/FRT (Thermo Fisher, Waltham, MA) between the FseI and AscI sites were used to transiently transfect 293T cells to validate the anti-SNED1 antibody generated in this study (Supplementary Figure S1) ...
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bioRxiv - Microbiology 2021Quote: ... PE-conjugated mouse anti-HLA-DR (clone LN3, Invitrogen); PE-Cy7-conjugated mouse anti-CD11b (clone ICRF44 ...
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: ... or rabbit anti-HLA-B (ThermoFisher Scientific PA5-35345), or mouse anti-HLA-A ...