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Citations for Thermo Fisher :
301 - 350 of 10000+ citations for Glycerol Kinase 1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... followed by 1 hour of blocking with normal goat serum (NGS) (5%, Invitrogen, #10000C). Primary antibodies raised against NaV1.1 (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... After blocking for 1 hour in 2% bovine serum albumin (BSA; BP9701, Fisher Scientific) in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with Hoechst 33342 reagent (ThermoFisher, H3570, 1:2,000 in blocking buffer) for 10 min and then washed once with PBS prior to image acquisition ...
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bioRxiv - Neuroscience 2023Quote: ... followed by 1 hour incubation at RT with BlockAid blocking solution (Life Technologies B10710). Primary antibodies (V5 ...
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bioRxiv - Microbiology 2023Quote: ... 594 and 647 secondary antibodies (Life Technologies; 1:500 in saponin/BSA blocking buffer) for 1 h at room temperature in a light protected vessel ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were incubated with blocking solution (1% w/v BSA, Fisher Scientific, in DPBS) for 45 min at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... followed by Alexa Fluor 488 conjugated secondary antibody (Invitrogen; 1:1000 in blocking buffer). Actin filaments were labelled with Alexa Fluor 568 phalloidin (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... incubated for 1 hour in blocking solution containing Hoechst 33342 (Invitrogen; 5 µg/ml) and secondary antibodies labeled with AlexaFluor 488 or AlexaFluor 555 (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... slices were incubated with Alexa Fluor - conjugated streptavidin (1:1000 in blocking solution, Invitrogen) overnight at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... 10 μg/mL of E-cadherin functional blocking antibody (HECD-1, #13-1700, ThermoFisher) was added to each well ...
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bioRxiv - Zoology 2024Quote: ... Tissue samples were subsequently rinsed three times with DPBS again and then transferred onto microscope slides in mounting buffer containing 1:1 DPBS: glycerol and 1µg/mL 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI) (Molecular Probes, Eugene, OR) to stain cell nuclei in prepared tissues and examined under a Lumen Dynamics XCite™ 120Q Nikon fluorescence microscope (Nikon ...
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Fussing about fission: defining variety among mainstream and exotic apicomplexan cell division modesbioRxiv - Microbiology 2020Quote: ... Blocking was performed for 20 min with Superblock® T20 (PBS) blocking buffer (Thermo Scientific) with 1% (v/v ...
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bioRxiv - Neuroscience 2022Quote: ... The membrane was blocked with a blocking agent (SEA BLOCK Blocking, Thermo Scientific, Cat # UH2788881) for one hour at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... After blocking with Pierce Protein-Free (TBS) Blocking Buffer (200 μL/well; Thermo Fisher Scientific) at 21 °C for 3 h ...
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bioRxiv - Neuroscience 2019Quote: ... followed by blocking for endogenous avidin and biotin (avidin/biotin blocking kit, Invitrogen, MA, USA) per manufacturers instructions ...
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bioRxiv - Immunology 2023Quote: ... After blocking the slides for an hour in SuperBlockTM blocking buffer (Thermo Fisher cat#37515) the slides were incubated in antibody cocktail solution (Antibody Panel described in Supplementary file 1 ...
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bioRxiv - Biochemistry 2023Quote: ... cells were again washed twice with PBS followed by blocking with blocking buffer (DMEM (Gibco) +10% FBS ...
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bioRxiv - Immunology 2023Quote: ... 5% glycerol and then mixed at 9 volumes to 1 volume of 200× concentrate SYPRO orange (Thermo Fisher) diluted in the same buffer ...
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bioRxiv - Cell Biology 2022Quote: ... were added at a dilution of 1:500 in blocking solution containing 1:100 Phalloidin 594 (ThermoFisher #A12381) to cells and incubated at 4 °C overnight ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... secondary antibody at 1:1,000 in blocking buffer along with 1 μg/ml Hoechst 33342 (Thermo Fisher Scientific). After washing three times with PBS ...
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bioRxiv - Microbiology 2023Quote: ... ab6556)] were used at 1:1,000 in blocking buffer and detected with horseradish peroxidase (HRP)-conjugated secondary antibodies (1:5,000; ThermoFisher). Membranes were washed in TBST followed by addition of enhanced chemiluminescent (ECL ...
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bioRxiv - Pathology 2019Quote: ... the monolayer was treated with 40 nM NG25 (inhibitor of TGFβ-activated kinase 1; TAK1: Invitrogen, Carlsbad, CA, USA) for 24 h ...
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bioRxiv - Cancer Biology 2019Quote: ... 1 mM EDTA) to a final concentration of 100 µM and were radiolabeled with T4 Polynucleotide kinase (PNK, Invitrogen) in a reaction containing 1 mM ATP-γ-32P (Perkin Elmer ...
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bioRxiv - Neuroscience 2019Quote: ... harbouring the full-length cDNA coding for the human muscle 6-phosphofructo-1-kinase muscle isoform (PFK1-M)3 (accession number, NM_000289.1) using Lipofectamine LTX-PLUS Reagent (Life Technologies) according with manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Retinas were then washed five times with 1:1 blocking buffer in PBS for 20 min and incubated with Alexa Fluor streptavidin-conjugated antibodies (1:100; Life Technologies) for 2 hours at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... solubilized tryptic peptides were quantified using the Pierce™ Quantitative Fluorometric Peptide Assay (ThermoFisher) as reported in previous work [17] ...
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bioRxiv - Microbiology 2021Quote: Labeled peptides were fractionated by High pH Reversed-Phase Peptide Fractionation Kit (Thermo Scientific). The dried peptides were reconstituted and acidified with 0.1% TFA solution and loaded on the equilibrated ...
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bioRxiv - Cell Biology 2021Quote: ... Peptide quantitation was performed using Pierce Quantitative Colorimetric Peptide Assay Kit (Thermo Fisher, 23275) and 500 µg of each sample was retained for analysis by MS (pre-TAILS sample ...
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bioRxiv - Cell Biology 2022Quote: ... 2.Peptides were desalted using the Pierce™ Peptide Desalting Spin Columns (Thermo Scientific) according to the manufacture’s protocol no ...
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bioRxiv - Neuroscience 2019Quote: ... The peptide concentration was determined using the Pierce Quantitative colorimetric peptide assay (Thermo Fisher). The peptide concentration of the samples was adjusted to 140 μg/ml and analyzed using a Q Exactive HF-X mass spectrometer coupled to an Ultimate 3000 RSCLnano pump (Thermo Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... we measured resulting peptide concentrations with the Pierce Quantitative Fluorometric Peptide Assay (ThermoFisher, #90110); this step was not performed for the secretome samples ...
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bioRxiv - Systems Biology 2022Quote: ... The digested peptides were quantified using Pierce Quantitative Colorimetric Peptide Assay (Thermo Fisher Scientific), and stored at -80°C until the TMT labeling.
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bioRxiv - Neuroscience 2022Quote: ... Peptide samples were cleaned using Pierce™ Peptide Desalting Spin Columns (Cat#: 89852, ThermoFisher) and analyzed in duplicate by LC-MS/MS using a Thermo Easy nLC 1200-QExactive HF (Cat# ...
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bioRxiv - Neuroscience 2022Quote: ... and peptide concentrations were determined with Quantitative Colorimetric Peptide Assay (Thermo Fisher Scientific Inc.). TMT labeled sample LC-MS/MS acquisitions were performed using an EASY-nLC 1000 connected to an Orbitrap Eclipse Tribrid mass spectrometer (Thermo Fisher Scientific Inc.) ...
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bioRxiv - Neuroscience 2022Quote: ... Peptide concentrations were determined with the Quantitative Fluorometric Peptide Assay (Thermo Fisher Scientific Inc). A total of 10μg of peptide was prepared to label with TMTpro 16 plex reagents (Thermo Fisher Scientific Inc. ...
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bioRxiv - Biochemistry 2023Quote: ... Cross-linked peptides were desalted using Pierce peptide desalting spin column (Thermo Fisher Scientific) and dried ...
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bioRxiv - Microbiology 2023Quote: ... Peptides were quantified using a Pierce Colorimetric Peptide Quantification Assay Kit (Thermo Scientific, 23275). 50 μg of each sample was separated for multiplex proteomic analysis ...
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Pan-tissue mitochondrial phenotyping reveals lower OXPHOS expression and function across tumor typesbioRxiv - Biochemistry 2023Quote: Final peptides were resuspended in 0.1% formic acid for peptide quantification (ThermoFisher Cat# 23275) and dilution to a final concentration of 0.25μg/μL ...
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bioRxiv - Systems Biology 2023Quote: Peptide concentrations were determined using the Quantitative Fluorometric Peptide Assay (Thermo Fisher Scientific, 23290) and 18 µg peptides per time point were subjected to TMT labeling ...
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bioRxiv - Neuroscience 2023Quote: Peptides were quantified and normalized using a quantitative colorimetric peptide assay (Thermo Fisher Scientific). TMT reagents were reconstituted with 41 μL of anhydrous acetonitrile with occasional vortexing for 5 min at RT ...
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bioRxiv - Biochemistry 2023Quote: ... Resulting peptides were desalted using Pierce™ peptide desalting spin columns (Thermo Fisher Scientific) according to manufacturer’s instructions and concentrated using a vacuum concentrator ...
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bioRxiv - Cell Biology 2023Quote: ... Peptide concentration of the supernatant was quantified using a quantitative colorimetric peptide assay (ThermoFisher). For TMT labelling we used 2 different kits to label each of the TMT experiments included in this manuscript ...
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bioRxiv - Biochemistry 2023Quote: ... Tryptic peptides were loaded onto a 0.3 x 5mm peptide trap column (ThermoFisher Scientific) at 30 μL per minute for 15 minutes before switching in line with a 0.075 × 250 mm nano easy column (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Peptide concentration was measured using the Pierce™ Quantitative Fluorometric Peptide Assay (ThermoFisher: 23290) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The peptide concentration was determined using the Pierce™ Quantitative Peptide Assay (Thermo Scientific). Aliquots of 30 µg peptides were labelled using TMTpro 18-plex isobaric mass tagging reagents (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... The peptide concentration was measured by Pierce™ Quantitative Fluorometric Peptide Assay (ThermoFisher Scientific) in Cary Eclipse Fluorescence Spectrometer (Agilent ...
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bioRxiv - Molecular Biology 2023Quote: ... Blocking and antibody incubations were done using StartingBlock T20 (TBS) Blocking Buffer (cat# 37543; Thermo Scientific). Primary antibodies were used at 1:1000 except for anti-thrombospondin-4 and anti-TIMP-1 which were diluted at 1:100 ...
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bioRxiv - Molecular Biology 2023Quote: ... were used following endogenous biotin blocking using Biotin-blocking kit (Life Technologies Corporation/Thermo fisher, E21390). For Aza-peptide preparation ...
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bioRxiv - Molecular Biology 2023Quote: ... were used following endogenous biotin blocking using Biotin-blocking kit (Life Technologies Corporation/Thermo fisher, E21390). For Aza-peptide preparation ...
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bioRxiv - Microbiology 2019Quote: ... 10% tryptone-peptide broth (Gibco), 1% P/S ...