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Citations for Thermo Fisher :
201 - 250 of 4934 citations for GW Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... The amplification used primers running in opposite directions from the mutation site in the template Pik-2 in pCR8/GW/TOPO vector (Invitrogen). DNA templates were then eliminated by incubating the reaction with DpnI (New England Biolabs ...
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bioRxiv - Plant Biology 2021Quote: ... and Pik-2 mutated versions were generated by site-directed mutagenesis (see below) using appropriate Pik-2 template in pCR8/GW/TOPO (Invitrogen) with Golden Gate compatible overhangs ...
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bioRxiv - Biochemistry 2022Quote: Selected PgTAGLips were PCR amplified using cDNA as a template and cloned in pCR8/GW/TOPO TA Cloning vector (Invitrogen). All sequence confirmations were carried out with at least four to five colonies from each genotype ...
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bioRxiv - Plant Biology 2022Quote: ... the 5 kb KYP genomic DNA sequence containing the 2 kb KYP native promoter was PCR-amplified and cloned into the pCR8/GW/TOPO vector (Invitrogen), then recombined into a modified pEarleyGate302 vector containing the 3xFLAG tag or PMDC107 vector with the mGFP tag.
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bioRxiv - Plant Biology 2022Quote: ... The ORFs were then swapped into the episomal yeast expression vector pDRf1-GW 56 by the LR clonase II (Invitrogen) reaction following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... Promoter entry clones were linked to entry clones containing their respective HA-PpCSLD coding sequences and inserted into pSi3(TH)GW (Tran and Roberts 2016b) using LR Clonase II Plus (Invitrogen). These vectors target the expression cassettes to the intergenic 108 locus ...
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bioRxiv - Developmental Biology 2022Quote: ... Amplified DNA fragments were subcloned into entry vectors (pENTR/D-TOPO, K240020, ThermoFisher; or pCR8/GW/TOPO, K 250020, ThermoFisher) and then were inserted into pBP-Gal80Uw-6 (#26236 ...
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bioRxiv - Cell Biology 2022Quote: ... The PCR product and pDRF1-GW AKAR3-EV were digested using Kpn2I and XbaI (Thermo Fisher Scientific, Waltham, MA, USA). Afterwards ...
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bioRxiv - Plant Biology 2020Quote: Full-length cDNAs encoding SlG1-5 and PSY1-2 proteins without their stop codons were amplified by PCR and cloned via BP clonase into pDONR207 entry plasmid using Gateway (GW) technology (Invitrogen). Full-length sequences were then subcloned through an LR reaction into pGWB405 plasmid for subcellular localization assays ...
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bioRxiv - Plant Biology 2021Quote: The genomic ATPd DNA construct for complementation of the atpd-1 mutation was generated by amplifying the appropriate DNA fragment (2438 bp) with the primers listed in Table S2 and cloning the fragment into the entry vector pCR8/GW/TOPO (Invitrogen). The fragment was inserted into the pMDC32 binary vector (Curtis and Grossniklaus ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA interference plasmids were built using annealed DNA oligonucleotides (Supplementary Note 1) in the pcDNA6.2-GW/EmGFP plasmid (Block-iT™ miRNA expression kit from Invitrogen). Specific primers for pre-miRNA were designed using Life Technologies’ online tool (www.lifetechnologies.com/rnai) ...
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bioRxiv - Developmental Biology 2019Quote: ... The ERlucT-IRES-Rluc bicistronic expression cassette was then PCR amplified using primers listed in Table 1 and then subcloned using the pCR8/GW/TOPO TA Cloning Kit (Invitrogen), to produce the Gateway cloning-compatible ERlucT-IRES-Rluc-pCR8 middle entry vector ...
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bioRxiv - Genetics 2020Quote: ... EAC11-F: 5′-TTGAATTCGACTTCGACCGCGGCGTTTT-3′ and EAC12-R: 5′-TTGAATTCATGTCTTGGCCAGGGGAGAG-3 and cloned into the entry vector pCR8/GW/TOPO (Invitrogen). In the next step ...
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bioRxiv - Cell Biology 2022Quote: ... [contains aa 1-547] was cloned from a cDNA pool derived from HeLa cells and subcloned into pcDNA3/GW (Invitrogen) with a C-terminal tdTomato-tag ...
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bioRxiv - Cell Biology 2022Quote: ... The complementary DNA oligos were annealed to generate a double-stranded oligo and cloned into the linearized pcDNA6.2-GW/EmGFP expression vector (using the BLOCK-iT Pol II miR RNAi Expression Vector kit, Invitrogen, K493600). As a negative control ...
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bioRxiv - Plant Biology 2022Quote: The pZPR3:3xNLS-RFP reporter was created by first cloning the 3.2kb region upstream of the ZPR3 start codon into pCR8-GW (Invitrogen; NEB), followed by an LR reaction using a modified pGREEN binary vector with a Gateway cassette upstream of 3xNLS-RFP (Invitrogen) ...
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bioRxiv - Plant Biology 2023Quote: ... the genomic locus of GLK1 (including the 3867-bp promoter but excluding the stop codon) was amplified by PCR and subcloned into the pCR8/GW/TOPO TA cloning vector (Invitrogen). The insert was transferred into the binary destination vector pMDC107 by LR reaction (pGLK1:gGLK1-GFP::pMDC107) ...
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bioRxiv - Plant Biology 2022Quote: ... The purified PCR fragments were used for genotyping by Sanger sequencing at Virginia Tech Genomic Sequencing Center and cloned to the PCR8/GW/TOPO vector by TA cloning (Invitrogen) for molecular marker tests.
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bioRxiv - Genetics 2023Quote: ... The FOXM1/RHNO1 promoter region was amplified with iMax-II DNA Polymerase (INtRON) and the products cloned into the pCR8/GW/TOPO vector (Invitrogen). Primers used are listed in Supplementary Table 9 ...
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bioRxiv - Plant Biology 2023Quote: ... or DVL11 promoter fused to the DVL11 CDS were amplified and cloned in to the pCR8 vector using the pCR8/GW/TOPO TA Cloning Kit (Invitrogen). Then ...
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bioRxiv - Evolutionary Biology 2023Quote: The putative regulatory regions were cloned after PCR amplification on genomic DNA in the PCR8/GW/TOPO vector (Life Technologies). Using Gateway technology (Life Technologies) ...
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bioRxiv - Cell Biology 2023Quote: ... and MEAF6 were amplified by PCR using KOD One PCR Master Mix -Blue-(TOYOBO, KMM-201) and inserted into pcDNA3/GW with an HA or a FLAG tag (Invitrogen) or into pMAL-c6T (New England Biolabs) ...
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bioRxiv - Microbiology 2019Quote: ... 10% tryptone-peptide broth (Gibco), 1% P/S ...
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bioRxiv - Microbiology 2021Quote: ... Peptide desalting columns from ThermoFisher Scientific (Pittsburgh ...
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bioRxiv - Neuroscience 2021Quote: AQUA standard peptides (ThermoFisher Scientific) were spiked into digested mouse CSF samples ...
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bioRxiv - Neuroscience 2023Quote: ... Myc peptide (Thermo Scientific, 15265673) 500 μg/ml was used for to elute the precipitated samples.
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bioRxiv - Cancer Biology 2023Quote: ... or HA peptide (Thermo Fisher) diluted to 1mg/mL in TBS ...
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bioRxiv - Pathology 2022Quote: ... After blocking with SuperBlock (37515, Thermofisher), primary antibodies CD161 (ab259916 ...
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bioRxiv - Molecular Biology 2021Quote: ... Superblock (TBS) blocking buffer (ThermoFisher Scientific), paraformaldehyde 16% (PFA ...
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bioRxiv - Developmental Biology 2022Quote: ... blocking reagent (Thermo Fisher Scientific, B40922) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... blocking 10% horse serum (Gibco, USA) and incubation with rat anti-BrdU antibody (1:500 ...
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bioRxiv - Cell Biology 2019Quote: ... The peptide concentration was determined using a Pierce quantitative colorimetric peptide assay (Thermo Fisher Scientific). Approximately 150 μg of peptides for each sample was labeled with 200 μg of TMT-6plex reagent (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... Peptide concentrations were then determined using the Pierce™ Quantitative Colorimetric Peptide Assay (Thermo Scientific) and dried by vacuum concentration ...
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bioRxiv - Cell Biology 2021Quote: ... The peptides in all six sample were quantified using Pierce Fluorescent Peptide assay (Thermo Scientific).
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bioRxiv - Plant Biology 2022Quote: ... Peptide concentration was determined using a PierceTM Quantitative Colorimetric Peptide Assay kit (23275, Thermo Scientific). 100µg peptide aliquots were labelled using TMT10plexTM (90110 ...
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bioRxiv - Microbiology 2020Quote: ... Peptide quantitation was performed using BCA (Pierce Quantitative Colorimetric Peptide Assay) or Qubit (Thermo Scientific) system.
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bioRxiv - Cell Biology 2022Quote: ... the quantification of peptide was performed using Pierce Quantitative Colorimetric Peptide Assay (Thermo Fisher Scientific). Then ...
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bioRxiv - Biochemistry 2021Quote: ... peptide totals were measured using Quantitative Colorimetric Peptide Assay (Thermo Scientific Pierce, cat. no.: 23275).
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bioRxiv - Neuroscience 2019Quote: ... Peptide concentrations were determined using the Pierce Quantitative Colorimetric Peptide Assay (Thermo Scientific; Cat# 23275). Approximately 300 µg of peptide was recovered from each digest ...
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bioRxiv - Cell Biology 2021Quote: ... Peptides were ordered at Peptides and Elephants (Hennigsdorf, Germany) or from ThermoFisher (Waltham, MA, USA) as TFA salts and dissolved as 50 mM stock solution in DMSO or in ddH2O for GAP10 and GAP16 experiments in Figs 1 ...
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bioRxiv - Plant Biology 2020Quote: ... Peptides were then quantified using Pierce Quantitative Fluorometric Peptide Assay (Thermo Scientific, catalogue number 23290) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Peptides were fractionated using a Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Scientific) according to the manufacturer’s instructions with slight modifications ...
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bioRxiv - Microbiology 2020Quote: The concentrations of the peptide soups were measured with Quantitative Colorimetric Peptide Assay (Thermo Scientific). TMT10plex amino reactive reagents (0.8 mg per vial ...
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bioRxiv - Cell Biology 2021Quote: ... The resulting peptides were quantified using a quantitative colorimetric peptide assay kit (Pierce/Thermo Scientific) and an equal amount of each labelled with Tandem Mass Tag (TMT ...
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bioRxiv - Neuroscience 2023Quote: ... Peptide concentration of the supernatant was quantified using a quantitative colorimetric peptide assay (Thermo Fisher). For TMT labelling we used the TMT-11plex kit (Thermo Fisher)53 ...
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bioRxiv - Neuroscience 2023Quote: ... Peptide concentrations were determined using the Pierce Quantitative Fluorometric Peptide Assay (Thermo Fisher Scientific 23290) following the descriptions of the manufacturer ...
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bioRxiv - Plant Biology 2023Quote: ... Desalting of peptides was done using Peptide Desalting Spin Columns (Pierce, Thermo Scientific, United States) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The peptide concentrations were determined using the Pierce quantitative colorimetric peptide assay (Thermo Fisher Scientific). Approximately 25 µg of peptides for each sample was labeled with 0.2 mg of TMT-10plex reagents (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... The peptide concentration was measured using Pierce™ Quantitative Fluorometric Peptide Assay (Thermo Fisher Scientific) by following the manual.
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bioRxiv - Biochemistry 2022Quote: ... Peptide concentrations were determined using Pierce Quantitative Colorimetric Peptide Assay (cat# 23275, Thermo Fisher Scientific), and samples were frozen at −80°C until LC-MS data acquisition.