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Citations for Thermo Fisher :
251 - 300 of 9905 citations for Frizzled 8 Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Identified and characterized plasmids containing the ACE2 isoform open reading frames were transfected into the HEK-293 cells using TurboFect reagent (ThermoFisher Corp.). 24 hours post-transfection the cells were harvested and processed for analysis by western blot according to established procedures [21] ...
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bioRxiv - Cell Biology 2022Quote: ... Ciliogenesis was induced by culturing RPE1 cells in medium without serum during 48 hours. HEK 293 cells (kind gift from F. Causeret, Institut Imagine, Paris) were cultured in DMEM medium (ThermoFisher Scientific) supplemented with 10 % fetal calf serum and antibiotics as previously ...
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bioRxiv - Cell Biology 2021Quote: ... the HEK 293 BRI3BP-HA cell line was transfected with control or BRI3BP silencer select siRNAs with Lipofectamine RNAi Max (ThermoFisher Scientific), according to manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: ... DMEM (for A-431, HEK-293, NTERA-2, SH-SY5Y) or Ham’s F-12K (for A549) media (by Gibco or Euroclone) containing 10% fetal calf serum ...
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bioRxiv - Microbiology 2022Quote: 100µg of the pCI PR8 NP:WT-C-His or pCI PR8 NP:F346S-C-His plasmids were transiently transfected into 100mL cultures of HEK Expi-293-F cells with ExpiFectamine according to the company protocol (Thermo Fisher). The transfected cells were pelleted (1000rpm ...
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bioRxiv - Genomics 2021Quote: ... Reporter plasmids (2 μg) were transfected into HEK-293 cells in 6-well plates at ∼60-90% confluency using Lipofectamine 3000 (ThermoFisher Scientific). Cells were harvested after 1 day by aspirating the media and resuspending the cells in 1 mL Trizol reagent (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... HEK-293 cells were maintained at 37°C with 5% CO2 in DMEM/F12 medium (Gibco, Life Technologies, Grand Island, NY) supplemented with 10% heat-inactivated FBS (Gibco) ...
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bioRxiv - Microbiology 2020Quote: ... HEK-293 cells were maintained at 37°C with 5% CO2 in DMEM/F12 medium (Gibco, Life Technologies, Grand Island, NY) supplemented with 10% heat-inactivated FBS (Gibco) ...
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bioRxiv - Biochemistry 2022Quote: The pEG BacMam plasmids with WT or mutant Fpn or the empty plasmid were transfected into HEK 293S cells on black wall 96-well microplates coated with poly-D-lysine (Invitrogen/Thermo Fisher). After 2 days ...
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bioRxiv - Biochemistry 2022Quote: The pEG BacMam plasmids with HsFpn or the empty plasmid were transfected into HEK 293S cells on black wall 96-well microplates coated with poly-D-lysine (Invitrogen/Thermo Fisher). After 2 days ...
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bioRxiv - Cell Biology 2022Quote: ... MEFs were cultured in the same medium as HEK 293 cells but supplemented with 1x non-essential amino acids (Gibco, 11140050). All cells were kept at 37°C in a 5% CO2 humidified atmosphere.
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bioRxiv - Cell Biology 2023Quote: ... HEK 293-TrkB cells were treated with drugs prior to cell lysis with cold Pierce RIPA lysis buffer (Thermo Scientific, 89901) supplemented with protease inhibitor set I (Sigma ...
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bioRxiv - Cell Biology 2023Quote: For proximity ligation assay transfected HEK-293 cells were seeded in an appropriate dilution on Nunc Chamber Slides (Thermo Fisher, USA). After fixation in 4% paraformaldehyde for 15 min at RT ...
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bioRxiv - Bioengineering 2024Quote: ... and m6A-Tracer-NES plasmid (m6A-Tracer; addgene, #159607) were simultaneously transfected into HEK-293 cells using Lipofectamine 3000 Transfection Reagent (Invitrogen, #L3000008). After 48-h co-transfection ...
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bioRxiv - Bioengineering 2020Quote: Adherent human embryonic kidney cells (HEKs) were grown in DMEM (Gibco, Gaithersburg, MD) + 10% FBS ...
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bioRxiv - Genomics 2022Quote: Human HEK 293T cells (ATCC # CRL-3216) were grown in DMEM (ThermoFisher #11995073) supplemented with 10% FBS (ThermoFisher #A3840001 ...
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bioRxiv - Molecular Biology 2023Quote: ... Human HEK 293T Lenti-X cells were grown in DMEM + GlutaMax (ThermoFisher Scientific) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2024Quote: Human Embryonic Kidney (HEK) 293T cells were cultured in DMEM high glucose (Invitrogen), supplemented with 10% fetal bovine serum (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... Enzyme-linked immunosorbent assays (ELISAs) for human IL-8 were performed using the Human IL-8 ELISA Kit (ThermoFisher SCIENTIFIC) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Stable integration of CLIP-UPF1SL into human Flp-InTM T-RExTM-293 cells (Invitrogen) and subsequent maintenance of this stable line was previously described (Kishor et al ...
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bioRxiv - Immunology 2019Quote: ... Human and rabbit Abs were transiently expressed with the FreeStyle 293 Expression System (Invitrogen). Abs were purified using affinity chromatography (Protein A Sepharose Fast Flow ...
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bioRxiv - Immunology 2019Quote: ... Human and rabbit Abs were transiently expressed with the FreeStyle 293 Expression System (Invitrogen). Abs were purified using affinity chromatography (Protein A Sepharose Fast Flow ...
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bioRxiv - Molecular Biology 2021Quote: Flp-In T-Rex 293 (human, female, embryonic kidney, epithelial; Thermo Fisher Scientific, RRID:CVCL_U427) cells were cultured in high-glucose ...
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bioRxiv - Systems Biology 2020Quote: ... 293-H and Freestyle™ 293-F (Gibco) were obtained from Thermo Fisher Scientific and cultivated in 293 SFM II medium (Gibco ...
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bioRxiv - Cancer Biology 2022Quote: ... IL-8 Monocyte Recombinant Human Protein (ThermoFisher, PHC0884), and Recombinant Human CXCL1/GROα Protein (R&D Systems ...
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bioRxiv - Neuroscience 2023Quote: The plasmid for heterologous expression of fshr-1 in HEK cells was obtained by directionally cloning the fshr-1 cDNA into the pcDNA3.1/V5-His-TOPO vector (Invitrogen). The cDNA sequence of the fshr-1a gene isoform was amplified by PCR using cDNA from mix-staged wild-type C ...
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bioRxiv - Neuroscience 2021Quote: ... 3-3.5 µg prestin plasmids and 0.4 µg eGFP plasmid were transfected to HEK 293 cells using 10 µl of Lipofectamine ® 3000 (reagent 2:1 ratio; ThermoFisher Scientific) in 500-ml Opti-MEM (Life Technologies) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: HEK-293 cells or HEK-293 cells stably expressing PAR4-YFP were loaded with calcium sensitive dye (Fluo-4 NW, F36206, Life Technologies, Thermo Fisher) and aliquoted into cuvettes with Hanks buffered salt solution (HBSS containing Ca2+ and Mg2+) ...
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bioRxiv - Bioengineering 2021Quote: ... WS1 and HEK-293 cells were obtained from ATCC (Manassas, VA) and cultured in Eagle’s Minimum Essential Medium (Life Technologies, Carlsbad, CA) supplemented with 10% FBS ...
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bioRxiv - Neuroscience 2021Quote: ... HEK 293 cells or rNLS mice brains were lysed in lysis buffer (50 mM Tris-HCl, protease inhibitors cocktail (Thermo Fisher Scientific), phosphatase inhibitor (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... HEK-293 cells (ATCC) were maintained at 37°C under 5% CO2 in Dulbecco’s modified Eagle’s medium (Gibco, Cat. no. 12800-017) supplemented with 10% FBS (Gibco ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... HEK-293 cells were transferred with a density of 12000 cells per well in white 96-well plates (Thermo Scientific™ Nunc™ F96 MicroWell™ ...
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bioRxiv - Biochemistry 2022Quote: The pEG BacMam plasmids with WT or mutant Fpn or the empty plasmid were transfected into HEK 293S cells on black wall 96-well microplates coated with poly-D-lysine (Invitrogen/Thermo Fisher). After 2 days ...
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bioRxiv - Biochemistry 2022Quote: The pEG BacMam plasmids with HsFpn or the empty plasmid were transfected into HEK 293S cells on black wall 96-well microplates coated with poly-D-lysine (Invitrogen/Thermo Fisher). After 2 days ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting plasmids with Fpn or the empty plasmid were transfected into HEK 293S cells on 6-well plates with 293fectin™ transfection reagent (Invitrogen/Thermo Fisher) per the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: HeLa and HEK 293 cell lines were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Life Technologies, Thermo Fisher Scientific, Waltham MA, USA), while SH-SY5Y cells were cultured in 1:1 DMEM:Ham’s F12 (MSD ...
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bioRxiv - Immunology 2023Quote: ... Consensus and Vietnam matrix protein 2 (M2)-inducible HEK cells were generated using the Flp-In™ T-REx™ 293 Cell Line system (Invitrogen) and grown following the methods in the previous papers 53 ...
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bioRxiv - Genetics 2023Quote: ... Reverse transfection in HEK 293 cells was performed with 3 nM final siRNA concentration with Lipofectamine™ RNAiMAX (ThermoFisher, Cat. No. 13778075) according to manufactural procedure ...
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bioRxiv - Neuroscience 2023Quote: ... with fewer than 15 passages onto 12-well plates in HEK-293 growth medium (DMEM [Gibco # 10566-061] with 10% FBS [Gibco #16140-071] and 1x Pen Strep [Gibco #15070-63]) and transfected them when 50-70% confluent ...
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bioRxiv - Immunology 2023Quote: ... Resulting plasmid DNA was transiently transfected into HEK Expi293-F cell line using the ExpiFectamine™ 293 Transfection Kit (Thermo Fisher Scientific). Six days after transfection ...
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bioRxiv - Bioengineering 2023Quote: HEK 293 cells were cultured at 37 °C and 5% CO2 using DMEM high glucose without phenol red (Gibco, Thermo Fisher Scientific), supplemented with 10% fetal bovine serum ...
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bioRxiv - Bioengineering 2023Quote: HEK 293 cells were cultured at 37 °C and 5% CO2 using DMEM high glucose without phenol red (Gibco, Thermo Fisher Scientific), supplemented with 10% fetal bovine serum ...
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bioRxiv - Biochemistry 2024Quote: HEK-293 cells (ATCC, CRL-1573) were subcultured every 3-4 days in Dulbecco’s modified Eagle’s medium (Thermo Fisher Scientific, 41965-039) supplemented with 10% v/v fetal bovine serum (Sigma Aldrich ...
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bioRxiv - Bioengineering 2024Quote: ... HEK 293T cells were plated in fibronectin coated 8-well NUNC chambers (Thermo Scientific, Rochester, NY) at a cell density of 60,000 cells per well ...
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bioRxiv - Bioengineering 2021Quote: Human embryonic kidney (HEK)293T cells (ATCC, CRL-11268) were grown in DMEM (Gibco) plus 10% heat-inactivated fetal calf serum (FCS ...
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bioRxiv - Neuroscience 2021Quote: Human embryonic kidney (HEK) 293T cells were cultured in DMEM (Gibco, Thermo Fisher Scientific) supplemented with 10% FBS (Gibco ...
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bioRxiv - Neuroscience 2019Quote: Human epithelial kidney (HEK 293T) cells were maintained in Dulbecco’s Modified Eagle’s Medium (Gibco) supplemented with 10% fetal bovine serum (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: Human embryonic kidney (HEK) 293T cells were cultured in DMEM (Gibco, Thermo Fisher Scientific) supplemented with 10% FBS (Gibco ...
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bioRxiv - Cancer Biology 2021Quote: Human embryonic kidney (HEK)293T cells (ATCC, CRL-11268) were grown in DMEM (Gibco) plus 10% heat-inactivated fetal calf serum (FCS ...
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bioRxiv - Synthetic Biology 2021Quote: The human embryonic kidney (HEK) 293T cell line was cultured in DMEM medium (Invitrogen) supplemented with 10% fetal bovine serum (FBS ...