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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for Forkhead Box N1 FOXN1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: Skin tissues collected from newborn Foxn1−/− (Cby.Cg-Foxn1
/cmdb) and Foxn1+/+ (C57BL/6; B6) mice were incubated in dispase (6 U/ml; Life Technologies) overnight at 4°C ... -
bioRxiv - Synthetic Biology 2023Quote: ... The samples were incubated overnight with Paired Box 7 (PAX 7) antibodies (Invitrogen, USA) in blocking solution at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... and resazurin inside an anaerobic box (Thermofisher). The H-cell reactors were continuously purged with N2 gas to maintain anaerobic condition ...
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bioRxiv - Cell Biology 2019Quote: ... tagBFP-N1 construct (Invitrogen) was digested with AgeI and NotI and BFP was ligated into Lifeact-GFP construct ...
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bioRxiv - Neuroscience 2020Quote: ... E3 or E4 cDNA into the p-EGFP-N1 or p-mCherry-N1 vector (Invitrogen) between the XhoI and BamHI restriction sites ...
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bioRxiv - Microbiology 2021Quote: ... HMECs were transfected with pmCherry-N1-Gal3 or pmCherry-N1-Gal8 using Lipofectamine LTX (Invitrogen, A12621) and incubated at 37°C overnight ...
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bioRxiv - Immunology 2022Quote: FoxP3RBR-forkhead (19 μl, 0.25 mg/ml) was mixed with 20x Protein thermal shift dye (1 μl) (Thermo Scientific) in SEC buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Neuroscience 2021Quote: ... with mCherry from pmCherry-N1 (Invitrogen). All sequences were verified by DNA sequencing.
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A sound strategy for homology modeling-based affinity maturation of a HIF-1α single-domain intrabodybioRxiv - Biochemistry 2020Quote: ... pEGFP-N1-M1 and pEGFP-N1-M3 plasmids were transfected into cancer cell lines using LipofectamineTM 3000 (Invitrogen). Six hours after transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... 2009) was subcloned into pEYFP-N1 DEST plasmid to generate pEYFP-N1-FBXL5 using Gateway cloning system (Invitrogen). The human FBLX5 ORF with deletions from 666-691aa ...
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bioRxiv - Physiology 2021Quote: Mouse-anti Paired box protein Pax-6 (PAX6) (Invitrogen, Cat #21103049) (1:300 ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA was synthesized from the total RNA and used as a template for real-time relative quantitation for Foxn1 using commercially available probes and reagents (Invitrogen) on an Applied Biosystems 7500 Real Time PCR System.
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bioRxiv - Cell Biology 2023Quote: ... UGGT2-/- and UGGT1/2-/- cells were transfected with 1 µg Trop-2-pEYFP-N1 or Trop-2-Q118E-pEYFP-N1 plasmids using Lipofectamine 3000 DNA transfection reagent following the manufacturer’s instructions (Life Technologies). Mock-transfection using the pEYFP-N1 plasmid was used as a negative control.
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bioRxiv - Biochemistry 2023Quote: ... Munc18 DKO-PC12 cells were co-transfected with 1 μg pCI VAMP2-pHluorin and 1 μg of either pmEos3.2-N1 Munc18-1WT or pmEos3.2-N1 Munc18-1 R388A with 6.75 μL LipofectamineTM LTX with PLUS ReagentTM (ThermoFisher, Cat# 15338-100) into 3.5 cm petri dishes as per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... each device was placed in a GA-7 Magenta box (Fisher scientific, USA) with a customized watering system (Fig ...
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bioRxiv - Plant Biology 2023Quote: ... Each EcoFAB was then placed into a sterile Magenta box (Fisher Scientific, USA), to maintain sterile conditions during the experiment ...
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bioRxiv - Cell Biology 2021Quote: ... was generated by transfecting HEK293T cells with N1-TauRD (P301L/V337M)-EYFP and N1-TauRD (P301L/V337M)-mTurquoise2 plasmids using lipofectamine 3000 (Thermo Fisher Scientific). Cells expressing the constructs were selected by 1 mg ml−1 G418 antibiotic (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... and EF1α-mCherry-N1 plasmid (Thermo Fisher Scientific, Singapore) were isolated using the Monarch® Plasmid Miniprep Kit (New England BioLabs Inc. ...
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bioRxiv - Immunology 2021Quote: ... Insertion of single base pair changes for the generation of human and mouse FOXN1 variants was achieved by site-directed mutagenesis using the Phusion site-directed mutagenesis kit (Thermo Fisher Scientific).Specific primers for the introduction of single base pair deletions or single base pair changes were designed using the following software www.agilent.com/store/primerDesignProgram.jsp ...
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bioRxiv - Plant Biology 2021Quote: ... and GST-△F-box-AFF1 proteins were immobilized on GST beads (Glutathione Agarose; ThermoFisher). Immobilized agarose beads containing 2 μg GST ...
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bioRxiv - Cancer Biology 2023Quote: ... Data were normalized on TBP (TATA-Box Binding Protein) or GAPDH (Thermo Fisher Scientific). The relative quantity was determined using ΔΔCt by the CFX Maestro software (BioRad).
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bioRxiv - Microbiology 2023Quote: ... bags were put into the transportation box (AnaeroPack™ 7.0 L Rectangular Jar, Thermo Scientific). Afterwards ...
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bioRxiv - Immunology 2023Quote: ... Aliquots were gradually frozen using a commercial freezing box (Mr. Frosty, Nalgene, Thermo Fisher Scientific) at −80 °C for 24 h before being transferred to liquid nitrogen for long-term storage.
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bioRxiv - Evolutionary Biology 2024Quote: ... Electrophoresis was performed in an Owl™ D3-14 electrophoresis box (Thermo Scientific, MA, USA) containing 1X TBE buffer for 45 min at 130 V delivered from an Owl™ EC 300 XL power supply (Thermo Scientific ...
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bioRxiv - Immunology 2020Quote: ... TriLink) and with N1-methylpseudouridine-5’-triphosphate (m1ΨTP; Thermo Fisher Scientific) replacing uridine-5’-triphosphate (UTP)40 ...
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bioRxiv - Immunology 2022Quote: ... TriLink) and with N1-methylpseudouridine-5’-triphosphate (m1ΨTP; Thermo Fisher Scientific) replacing uridine-5’-triphosphate (UTP ...
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bioRxiv - Immunology 2020Quote: ... TriLink) and of N1-methylpseudouridine-5’-triphosphate (m1ΨTP; Thermo Fisher Scientific) instead of uridine-5’-triphosphate (UTP)35 ...
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bioRxiv - Microbiology 2020Quote: ... followed by treatment with RNAse A/N1 (100 µg/mL, Thermo Scientific), Proteinase K (100 µg/mL ...
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bioRxiv - Cancer Biology 2020Quote: ... in a slide box humidified using wet paper towels (Fisher Scientific Cat # 03-448-1 or -2) for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... cells were grown on TSA plates at 37°C in a sealed box with Anaerogen sachets (Oxoid ThermoFisher) for 24h ...
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bioRxiv - Evolutionary Biology 2024Quote: ... with plasmids expressing wildtype (WT) or mutant TRPM2 channel and pEGFP-N1 (Invitrogen) that expresses enhanced green fluorescent protein (GFP ...
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bioRxiv - Plant Biology 2021Quote: ... 100 g were transferred into a previously sterilized Magenta GA-7 plant culture box (Thermo Fisher Scientific, Schwerte, Germany), sealed and autoclaved again ...
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bioRxiv - Microbiology 2021Quote: ... TATA-box binding protein (TBP) (Hs00427620_m1) and beta-2-microglobulin (B2M) (Hs00187842_m1) was used (all from Thermo Fisher Scientific). PCR was performed using the LightCycler 480 Instrument (Roche Life Science ...
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bioRxiv - Molecular Biology 2021Quote: ... pEGFP-N1 and insert were digested with AflII (Thermo Fisher Scientific, Waltham, Massachusetts, US). Digested vector was dephosphorylated with Alkaline Phosphatase (New England Biolab ...
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bioRxiv - Cancer Biology 2022Quote: ... and 0.1 μg of pDsRed-N1 as internal control by Turbofect (Thermo Fisher Scientific). After 6 hours ...
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bioRxiv - Microbiology 2021Quote: ... N1 #6 or N3 #2.13 cells using Lipofectamine 2000 transfection reagent (Invitrogen, ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... N1 #6 or N3 #2.13 cells using Lipofectamine 2000 transfection reagent (Invitrogen, ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: 20,000 freshly dissected sporozoites were pre-incubated with pathogen box compounds at 1 μM concentration and 1:1,000 diluted live/dead fixable green stain (Invitrogen, L23101) in HBSS with 1% BSA (pH 7.4 ...
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bioRxiv - Microbiology 2023Quote: ... particles were then picked using the template picker and extracted from dose-weighted micrographs using a box size of 220 x 220 pixels2 for the Glacios (ThermoFisher) dataset and 300 x 300 pixels2 for the Titan Krios (ThermoFisher ...
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bioRxiv - Immunology 2024Quote: ... The membranes were placed in an opaque gel box and incubated with blocking buffer (PBST + 10% Fish gelatin (PBS 10X, pH 7.2, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... All aerobic cultures were incubated with shaking at 180 rpm while anaerobic cultures were incubated statically in an air-tight box with AnaeroGen anaerobic gas generating sachets purchased from Thermo Scientific. OD600 nm measurements were made using a Thermo Scientific Multiskan GO spectrophotometer and percentage growth of n = 3 biological replicates (unless stated otherwise ...
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bioRxiv - Microbiology 2024Quote: ... and electrophoresis was run for 45 minutes at 80 V, whereafter the DNA fragments were visualised using G: box (SYNGENE, USA) and UV-illuminator (Invitrogen, USA).
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bioRxiv - Microbiology 2022Quote: ... washed again, and developed using a Syngene G-Box XT4 system (Frederick, MD) after exposure to a chemiluminescent substrate (ThermoFisher Supersignal Plus).
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bioRxiv - Neuroscience 2021Quote: ... or pEGFP-N1-TG2 vector (Furini et al., 2018) using 6 μl of Lipofectamine2000 (Thermo Fisher Scientific) in a total volume of 100 μl per slide (about 1.7×105 neurons) ...
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bioRxiv - Molecular Biology 2021Quote: ... the DNA encoding RBD203-N1 was synthesized and subcloned into the Pichia secretory expression vector pPICZαA (Invitrogen) using EcoRI/Xbal restriction sites (GenScript) ...
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bioRxiv - Immunology 2022Quote: ... The expression of the N1 gene was performed using specific primers and probes TaqMan Assay (Applied BioSystems, Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: The mRNA content of AMPKγ3 and TATA-box binding protein (TBP) was determined by real-time PCR using the QuantStudio 7 Flex detection system (Applied Biosystems, CA, USA) as previously described (33) ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by 1 h incubation at 25 °C in a light-tight box with Streptavidin-Cy3 (1 μg/mL, Thermo Fisher Scientific, 10768023) and fluorescent secondary antibody Alexa Fluor 488 donkey anti-mouse (Invitrogen ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The reactions were then loaded onto a 1% agarose gel and electrophoresed in an Owl™ D3-14 electrophoresis box (Thermo Scientific, MA, USA) containing 1X TBE buffer for 30 min at 180 V delivered from an Owl™ EC 300 XL power supply (Thermo Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... tumor necrosis factor α (TNF-α) and TATA box binding protein (TBP) were determined by real☐time PCR (ABI 7900 Prism, Applied Biosystems, US). Sequences used to amplify a fragment of IL-6 were FP ...