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Citations for Thermo Fisher :
351 - 400 of 10000+ citations for Fish Sperm DNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: The sperm MMP was evaluated using two fluorescent stains containing Rhodamine-123 (R123; Invitrogen TM, Eugene, OR, USA) and propidium iodide (PI ...
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bioRxiv - Cell Biology 2021Quote: ... pelleted sperm cells were resuspended in 540 μL of PBS supplemented with disuccinimidyl sulfoxide (DSSO, Thermo Fisher Scientific) to a final concentration of 1 mM ...
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bioRxiv - Neuroscience 2021Quote: ... All FISH embryos were mounted using DAPI in MOPs buffer and NucBlue (ThermoFisher Scientific, Waltham, MA). Images were taken using the Zeiss LSM 880 scanning confocal microscope (Carl Zeiss Incorporation ...
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bioRxiv - Developmental Biology 2022Quote: ... fish were subjected to caudal fin amputation and administrated with Ethynyl-2’-deoxyuridine (EdU, Thermo Scientific: C10337 ...
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bioRxiv - Genomics 2020Quote: RNA-FISH performed in this study was based on the ViewRNA ISH (Thermo Fisher Scientific, QVC0001) protocol with minor modifications ...
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bioRxiv - Immunology 2021Quote: 16S rRNA FISH was performed using the view RNA tissue assay core kit (Thermo Fisher #19931) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... The Alexa Fluor 488 - labeled RNA probe was obtained using the FISH Tag RNA Kit (Invitrogen). In the first step ...
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bioRxiv - Neuroscience 2022Quote: ... and 4 old (7 months) male fish were dissected immediately into cold PBS (pH 7.4, Gibco). The dissected brains were fixed with 4% paraformaldehyde (PFA ...
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bioRxiv - Neuroscience 2022Quote: ... the fish larvae were immobilized by bath application of α-Bungarotoxin (B1601, Fisher Scientific, 1mg/ml) dissolved in external solution (in mM ...
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bioRxiv - Microbiology 2021Quote: ... FISH samples were mounted with Prolong Gold agent containing DAPI (Molecular Probes, Fisherscientific, Ottawa, ON Canada). The probes for Methylophaga spp ...
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bioRxiv - Molecular Biology 2023Quote: ... gill tissue was extracted from each larval fish and preserved in RNAlater (Invitrogen; Carlsbad, CA, USA), held at 4°C for 24 h and stored at −80°C prior to RNA extraction ...
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bioRxiv - Neuroscience 2023Quote: For lightsheet experiments 6-7 dpf fish are placed in 2.2% low-melting point agarose (Thermofisher) in a chamber optimized for our lightsheet microscope.
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bioRxiv - Systems Biology 2023Quote: ... The fish were fixed in pre-chilled 10% neutral buffered formalin (NBF) (Fisher Scientific, Allentown, PA) overnight at room temperature (Lin et al ...
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bioRxiv - Zoology 2023Quote: The total RNA of the whole fish was extracted using TRIzol® reagent (Invitrogen, California, USA) according to the manufacturer’s instructions ...
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bioRxiv - Zoology 2023Quote: ... Gill tissue was then extracted from each fish and preserved in RNAlater (Invitrogen; Carlsbad, CA, USA), held at 4°C for 24 h and stored at −80°C prior to RNA extraction ...
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bioRxiv - Developmental Biology 2023Quote: ... RNA was isolated from brain tissues of wild-type and scn8abEGFP fish using Tri-Reagent (ThermoFisher). Complementary DNA (cDNA ...
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bioRxiv - Molecular Biology 2024Quote: ... Coverslips were incubated with FISH probe incubation buffer (2x SSC, 40ng/μL yeast tRNA (Life Technologies), 40ng/μL salmon sperm DNA (Life Technologies) ...
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bioRxiv - Cell Biology 2020Quote: Freshly ejaculated duck sperm were incubated for 20 min with mitochondrial potential dye Mitotracker-Red (5 μM; Life Technologies) at 37 °C and then fixed with 1:1 ratio (v/v ...
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bioRxiv - Developmental Biology 2019Quote: Total RNA was extracted from purified sperm using a slight modification of a standard Trizol protocol (Thermo Fisher Scientific). In brief ...
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bioRxiv - Developmental Biology 2022Quote: ... The whole sperm protein extract thus obtained was quantified using Pierce™ 660nm protein assay reagent (Thermo Scientific™) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2019Quote: Total RNA from whole retinas was extracted using TRIzol (6 retinas from 3 fish per sample) (Invitrogen). RNA was quantified using Nanodrop spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... qPCR for fish samples were performed by primers in combination with Power SYBR Green (Thermo Fisher Scientific) using a ViiA7 real-time PCR system (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2019Quote: SABER FISH of retinal sections was carried out on Superfrost Plus slides (Thermo Fisher Scientific, cat. #1255015) using an adhesive hybridization chamber (Grace Bio-Labs ...
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bioRxiv - Microbiology 2019Quote: ... The fish were weighed and tissue samples were taken and stored in RNAlater® solution (Ambion, USA) at −80 °C until use ...
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bioRxiv - Microbiology 2023Quote: ... 20 % formamide] and 2 μl of EUB 338 FISH probe (GCTGCCTCCCGTAGGAGT) conjugated to Alexa Fluor 594 (Thermofisher) were added to the samples and incubated at 46 °C overnight (18-24 hrs) ...
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bioRxiv - Biophysics 2021Quote: Tilt series of whole or cryo-FIB milled mouse sperm flagella were acquired using a Titan Krios (Thermo Fisher Scientific) operated at 300 keV with post-column energy filter (Gatan ...
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bioRxiv - Evolutionary Biology 2022Quote: Females were mated as described in the “Sperm transfer and displacement” section and dissected in Grace’s supplemented insect medium (Thermo Fisher) under CO2 anesthesia 3-4 h AESM ...
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bioRxiv - Bioengineering 2021Quote: Viability of the bioprinted fibers was assessed after 24 hours using a LIVE/DEAD™ Sperm Viability Kit (Invitrogen, L7011) as per the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Discs were transferred to hybridization buffer (10% w/v dextran sulfate, 4X SSC, 0.01% w/v salmon sperm ssDNA (Invitrogen 15632), 1% v/v vanadyl ribonucleoside (NEB S14025) ...
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bioRxiv - Cell Biology 2021Quote: Sperm from Tcfl5+/+ and Tcfl5+/− mice were extracted and incubated in Hanks’ Balanced Salt Solution (HBSS) medium (14175-079, Gibco) at 37°C for 20 min ...
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bioRxiv - Physiology 2022Quote: ... Fixed sperm cells were pipetted into square “windows” drawn with a hydrophobic pen on glass microscope slides (Thermo Fisher Scientific), which were incubated at 4°C for 30 minutes and then washed in ice cold phosphate buffered saline (PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... The 3’ ends of the sperm and HEK293 TFAM mRNA species were mapped by 3’ RLM-RACE using the FirstChoice RLM-RACE Kit (Ambion) with modifications ...
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bioRxiv - Developmental Biology 2023Quote: ... The maturity oocytes were subjected to intracytoplasmic sperm injection (ICSI) immediately and then cultured in CMRL-1066 medium (Gibco, 11530037) containing 10% FBS at 37°C in 5% CO2 ...
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bioRxiv - Developmental Biology 2023Quote: ... Spermatozoa were centrifuged at 15,300 g for 2 min and the sperm pellet was lysed in TRIzol (Thermo Fisher Scientific). Chloroform was added to the lysate and incubated for 5 min at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... and Vm by loading sperm samples with the corresponding dyes (Fluo-4AM, BCECF-AM, or pHrodo Red-AM from Molecular Probes, Eugene ...
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bioRxiv - Developmental Biology 2021Quote: ... DNA and RNA were extracted simultaneously from the frozen antheridium halves and individually from sperm using the Dynabeads® mRNA DIRECTTM Micro Kit (Invitrogen). Embryos were dissected from fertilized gametophores 7 days following sperm application and the surrounding pseudoperianths were removed ...
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bioRxiv - Molecular Biology 2020Quote: ... and diluted to 30 pg/μl concentration in 5 mM Tris-HCl (pH 7.5) supplemented with 5 ng/µl carrier herring sperm (Thermo Fisher Scientific). The MSI assay was performed using single-molecule PCR (SM-PCR ...
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bioRxiv - Evolutionary Biology 2020Quote: Fish were euthanized following authorised protocol and dissected brains were kept in RNALater (Ambion Inc., Austin, TX, USA). We extracted total RNA from the brains of three infected ...
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bioRxiv - Cancer Biology 2019Quote: ... Immunoprecipitation was performed with the FISH-antibody (Anti-ATM, rabbit polyclonal, Novus) and magnetbeads (Dynal Biotech ASA/Invitrogen). The proteins were separated by gel-elektrophoresis ...
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bioRxiv - Evolutionary Biology 2020Quote: ... fish were humanely euthanized in a lethal dose of buffered MS-222 and tissue preserved in RNAlater (Invitrogen). Photographs were analysed using the QCPA framework ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA-FISH was carried out using the Affymetrix View ISH Cell Assay Kit and custom probes (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... Probes were diluted to 100nM each in RNA FISH Hybridization Buffer (Stellaris) containing 10% formamide (v/v) (ThermoFisher), then incubated on the fixed and permeabilized cells using 100μL/well for 4hrs at 37°C ...
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bioRxiv - Physiology 2020Quote: ... Individual fish identified as potential mutants by HRMA were further confirmed by Topo-TA cloning (Thermo Fisher Scientific) of the target locus ...
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bioRxiv - Pathology 2022Quote: Three pooled samples of diseased fish (3 individuals/pool) were subjected to RNA extraction using Trizol reagent (Invitrogen) according to manufacturer’s protocol and DNA extraction using lysis buffer containing 2% sodium dodecyl sulphate (SDS ...
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bioRxiv - Immunology 2019Quote: ... mRNA was isolated from immunoprecipitated polysomes from 50 pooled wounded fish or unwounded controls using TRIzol reagent (Invitrogen). 4 paired replicates were collected ...
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bioRxiv - Physiology 2021Quote: ... DNase-free total RNA (0.5 μg) was reverse-transcribed using Verso cDNA kit (Thermo Fisher Scientific; naïve fish) or High Capacity cDNA Reverse Transcription kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: RNA-FISH was performed using the RNA View Cell Plus assay kit (Cat. No.88-19000-99, ThermoFisher) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: Slices were incubated in blocking buffer solution (1% bovine serum albumin, 0.2 % fish skin gelatin, 0.1 % Triton, or 1 % Triton in 1x PBS; BSA from Gibco, Thermo Fisher Scientific ...
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Treg cells drive MYCN-mediated immunosuppression and tumor aggressiveness in high-risk neuroblastomabioRxiv - Cancer Biology 2023Quote: ... The frozen fish were embedded in O.C.T compound (SAKURA) and sectioned at a 14 µm thickness using a cryostat (ThermoFisher).
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bioRxiv - Genomics 2023Quote: ... All fish were genotyped using AquaGen’s custom Axiom®SNP (single-nucleotide polymorphism) genotyping array from Thermo Fisher Scientific (former Affymetrix ...