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Citations for Thermo Fisher :
251 - 300 of 10000+ citations for Fatty acid binding protein FABP1 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... Total protein concentration was determined by using the bicinchoninic acid (BCA) protein assay (Thermofisher scientific) and measured with a spectrophotometer (Glomax ...
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bioRxiv - Cell Biology 2019Quote: ... Sample protein content was determined by the BiCinchoninic Acid Assay protein assay (Thermo Fisher Scientific). Extracts were analyzed by SDS-PAGE using a 10% Bis–Tris Nu-PAGE gels (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... and lysate protein concentration was determined using the Pierce Bicinchoninic Acid Protein Assay Kit (Invitrogen) using a standard curve created with BSA standards of known concentration ...
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bioRxiv - Pathology 2022Quote: The protein concentration was determined using the bicinchoninic acid Protein Assay Reagent (Thermo Fisher Scientific). Lysates were mixed with an equal volume of 2× sodium dodecyl sulfate (SDS ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein concentration was measured using a PierceTM bicinchoninic acid (BCA) protein assay kit (Thermo Scientific) per manufacturer recommendations ...
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bioRxiv - Immunology 2020Quote: ... The protein concentration was detected with a protein bicinchoninic acid (BCA) kit (Thermo Fisher Scientific). Samples were analyzed with a mouse XL cytokine array kit (R&D Systems) ...
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bioRxiv - Microbiology 2020Quote: ... Protein concentrations were determined using the bicinchoninic acid (BCA) protein assay (ThermoFisher Pierce, Waltham, MA). Urea and dithiothreitol (DTT ...
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bioRxiv - Neuroscience 2019Quote: ... Protein quantitation was performed using bicinchoninic acid (BCA) Protein Assay Kit (Thermo Scientific, Rockford, IL); 100 µg of each sample was used for TMT labeling.
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bioRxiv - Neuroscience 2020Quote: ... Proteins concentrations were quantified using the Bicinchoninic acid (BCA) protein assay kit (Thermo Scientific, France). Ten micrograms of proteins was loaded on a 4-20% precast polyacrylamide gels (Mini Protean gels ...
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bioRxiv - Immunology 2021Quote: ... The protein concentration was measured with commercially available bicinchonic acid (BCA) protein assay kit (ThermoFisher) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... sonicated for 10 s and protein amounts normalized following a bicinchoninic acid protein assay (ThermoFisher). Samples were diluted to 1.0 mg/mL protein using homogenization buffer and incubated with 20 U/μL lambda phosphatase in MnCl2 and enzyme buffer as supplied with the lambda protein phosphatase kit (New England Biolabs ...
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bioRxiv - Neuroscience 2022Quote: ... Protein content was determined by the bicinchoninic acid method (BCA protein assay kit, ThermoFisher Scientific). We resolved a total of 50 µg of protein by SDS-PAGE ...
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bioRxiv - Microbiology 2023Quote: ... The protein content was assessed by the bicinchoninic acid (BCA) protein assay (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... and protein concentrations were measured with the bicinchoninic acid protein assay kit (Thermo Fisher Scientific). Equal amounts of total protein were resolved on Tris-glycine gels (Invitrogen ...
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bioRxiv - Physiology 2023Quote: ... The protein concentration was determined using the bicinchoninic acid protein assay (Thermo Fisher Scientific, EUA). Lysates were separated by sodium dodecyl sulfate‒polyacrylamide gel electrophoresis (SDS‒PAGE ...
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bioRxiv - Microbiology 2023Quote: ... The protein concentration was determined using a bicinchoninic acid (BCA) protein assay kit (Thermo Scientific), and the yield was determined to be approximately 5 mg/100 mL of bacteria culture.
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bioRxiv - Microbiology 2023Quote: ... and lysate protein concentration was determined using the Pierce Bicinchoninic Acid Protein Assay Kit (Invitrogen) using a standard curve created with BSA standards of known concentration ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration was assessed using the Pierce Bicinchoninic acid (BCA) protein assay (Thermo Fisher #23225). Unless otherwise stated ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... extended with a nucleic acid sequence encoding for 6 histidine residues (His-tag) and cloned into the mammalian expression vector pcDNA3.1(+) (Invitrogen). The soluble antigen was produced by transient gene expression in CHO cells as described previously [38] and purified from the cell culture medium by Ni-NTA resin (Roche) ...
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bioRxiv - Biochemistry 2020Quote: A 5-mL HiTrap™ Protein G HP column (Cytiva) was equilibrated with 10 CVs of Protein G IgG Binding Buffer (pH 5.0; Thermo Fisher Scientific). Spent medium containing CR3022 mAb was diluted with binding buffer at a 1:1 volume ratio and filtered using a 0.22 μm stericup ...
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bioRxiv - Cell Biology 2020Quote: Plasmids encoding for recombinant His-tagged proteins were transformed into Escherichia coli BL21(DE3) (Invitrogen). Bacterial cultures were grown at 37°C in LB media to OD600 of 0.6-0.8 and induced with 0.5 mM IPTG at 18 °C for 16h (overnight) ...
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bioRxiv - Cell Biology 2019Quote: ... The His-mCherry-fusion proteins were purified using a Ni-NTA column (Thermo Fisher Scientific). The eluted proteins were dialyzed in 1 L dialyzed buffer (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: ... His-tagged proteins were visualised using a primary anti-6xhis antibody (ArtNr: 11533923, Fisher Scientific), a HRP-coupled secondary antibody (ArtNr ...
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bioRxiv - Neuroscience 2023Quote: ... The His-tagged dAsapPZA protein was expressed and purified following the manufacturer’s guidelines (Invitrogen, USA).
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bioRxiv - Bioengineering 2021Quote: The HHH peptide (Cys-biotin-PEG2-Gly-His-His-His-Gly(COOH)) was purchased from Thermo Fisher. It was 84% pure as determined by mass spectrometry ...
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bioRxiv - Molecular Biology 2024Quote: The cDNA sequence of human Sph1 was subcloned from the pcDNA3.1/V5-His-TOPO expression vector (Invitrogen, USA) 62 into the Venus-BiFC plasmids previously described 37 ...
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bioRxiv - Cancer Biology 2022Quote: ... (FBS HI; Invitrogen) and 1% Glutamax (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... anti-His (Invitrogen), or anti-FLAG (Sigma ...
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bioRxiv - Immunology 2022Quote: The Invitrogen ProtoArray® Human Protein Arrays (ThermoFisher Scientific) are high-density microarrays that contain more than 9,000 unique human proteins individually purified and arrayed onto a nitrocellulose-coated slide ...
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bioRxiv - Bioengineering 2023Quote: ... and recombinant human interleukin-2 (IL-2) protein (Invitrogen) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... The media was diluted with an equal volume of Protein A IgG Binding Buffer (Thermo Scientific) and loaded onto the column ...
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bioRxiv - Physiology 2022Quote: ... 18S ribosomal RNA (18S) and TATA-binding protein (Tbp).The primers (Thermofisher Scientific, Waltham, MA, USA) were derived from mouse sequences and are listed in supplemental methods (supplement table 2.).
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bioRxiv - Molecular Biology 2021Quote: Putative ac4C binding proteins were subjected to SDS PAGE on Novex Tris-Glycine 4-20% (Invitrogen) gel followed by either silver staining with Pierce Silver Stain Kit (Thermo ...
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bioRxiv - Physiology 2019Quote: ... Tadalafil plasma protein binding was also determined by mass spectrometry following rapid equilibrium dialysis (ThermoFisher, UK). K2EDTA plasma samples (100 µl ...
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bioRxiv - Immunology 2021Quote: ... S protein binding was detected using 25 µL/well of PNPP (p-nitrophenyl phosphate;Thermo Fisher) and the reaction was measured at a wavelength of 405 nm by the Varioskan Lux Reader (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... The DNA-protein binding reactions were performed according to the manufacturer’s instructions (Thermo Fisher Scientific, USA). The 4% polyacryl gel was used to separate the DNA-protein complexes ...
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bioRxiv - Cell Biology 2020Quote: ... KKT4 proteins were buffer-exchanged into the binding buffer using Zeba spin desalting columns (Thermo Fisher). KKT42–645 (0.67 µM ...
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bioRxiv - Immunology 2021Quote: Nunc MaxiSorpTM high protein-binding capacity 96 well plates (Thermo Fisher Scientific, Cat# 44-2404-21) were coated overnight at 4 °C with 1µg/ml SARS-CoV-2 RBD ...
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bioRxiv - Bioengineering 2020Quote: ... The flavin binding fluorescent protein gene was synthesized from previously known sequence (56) (Thermo Fisher Scientific) and the plasmid was formed using the hot fusion method ...
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bioRxiv - Immunology 2022Quote: ... Supernatant with positive RBD binding signals was next purified using Protein A/G beads (Thermo Scientific), concentrated using a 30 kDa or 100 kDa cut-off concentrator (Vivaspin ...
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bioRxiv - Neuroscience 2022Quote: JD1 antibody was adsorbed to high protein binding Nunc-Immuno MaxiSorp 96-well plates (Nunc, Roskilde,) at 4°C for 4hrs ...
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bioRxiv - Cell Biology 2023Quote: All steps from here on were performed in Low protein binding microcentrifuge tubes (Thermo Scientific, 90410). Bead preparation and antibody binding ...
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bioRxiv - Cell Biology 2024Quote: ... Antibody binding was followed by incubation with Protein A/G magnetic beads (Thermo Fisher Scientific, USA) for 1h at 4°C on a rotator ...
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bioRxiv - Cell Biology 2021Quote: ... 5 mM MgCl2) for cell membrane binding proteins (LPL, Caveolin 1) and quantified with Pierce™ BCA Protein Assay Kit (Buffer (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2024Quote: rox2RNA probes at 100 nM and hrp38 RNA probes at 50nM were incubated with MBP-tagged FL CLAMPWT protein or MBP-tagged FL CLAMPdelPrLD protein in REMSA binding buffer provided with the LightShift Chemiluminescent RNA EMSA kit (Thermo Scientific, USA) at room temperature for 30 min according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... Protein concentration was determined using the Pierce® Bicinchoninic Acid Protein Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2021Quote: ... Lysate protein concentrations were quantified using a bicinchoninic acid (BCA) protein assay kit (Thermo scientific, #23225). Equal total protein concentrations were resolved on SDS-polyacrylamide gels (BioRad ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentrations were determined using a Bicinchoninic Acid Protein (BCA) Assay (Thermo Fisher Scientific, Waltham, MA), and subsequently 100 μg of protein from each sample were aliquoted and samples were brought to an equal volume using water ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein concentrations were determined using a bicinchoninic acid protein (BCA) Assay (Thermo Fisher Scientific, Waltham, MA), and subsequently 1-2 mg of protein from each sample were brought to an equal volume using a solution of 8 M urea in 50 mM TEAB ...
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bioRxiv - Biochemistry 2020Quote: ... Protein concentrations were determined by bicinchoninic acid assay (BCA protein assay kit, Pierce Thermo Fisher Scientific). Whole cell lysates were separated on 7.5% Criterion TGX 7.5% SDS-PAGE gels (Bio-Rad ...