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Citations for Thermo Fisher :
151 - 200 of 10000+ citations for Fab ZAP rabbit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: All antibodies in IgG and Fab forms in this study were transiently expressed in ExpiCHO cells (Thermo Fisher). For IgGs and Fabs ...
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bioRxiv - Microbiology 2020Quote: Polyclonal anti-CD55 antibody and control IgG were digested using the Pierce™ Fab preparation kit (Thermo Fisher Scientific). The resulting Fab fragments were quantified on a spectrophotometer ...
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bioRxiv - Immunology 2022Quote: ... The antigen binding fragment (Fab) portion of LY-CoV1404 was generated by proteolytic digestion using immobilized papain (ThermoFisher Scientific), followed by removal of un-cleaved protein using standard chromatography techniques ...
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bioRxiv - Immunology 2022Quote: Intact masses of the eluted IgA1 Fabs were retrieved from the generated RAW files using BioPharmaFinder 3.2 (Thermo Scientific). Deconvolution was performed using the ReSpect algorithm between 5 and 57 min using 0.1 min sliding windows with a 25% offset and a merge tolerance of 30 ppm ...
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bioRxiv - Molecular Biology 2021Quote: ... 012-22384 Anti DYKDDDDK mouse IgG2b) using the Pierce Mouse IgG1 Fab and F(ab’)2 Preparation Kit (ThermoFisher). First ...
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bioRxiv - Immunology 2023Quote: ... Prior to experiments both Fab and 6P spike were buffer-exchanged into HEPES-buffered saline (HBS, pH 7.4) using Zeba spin columns (ThermoFisher).
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bioRxiv - Molecular Biology 2023Quote: ... The RRtoGG αEβ7 ectodomain was diluted 1:10 in non-activating buffer and incubated with digested LF61 Fab (Invitrogen) at a 1:4 molar ratio for 45 minutes at room temperature with end-over-end mixing ...
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bioRxiv - Bioengineering 2024Quote: ... Alexa Fluor 647-conjugated anti-Fc Fab (Surface protein and CYpHER quantitation, ThermoFisher Zenon Labeling Kits, mouse IgG2a for anti-EGFR 199.12 quantitation [ThermoFisher Z25108] ...
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bioRxiv - Biochemistry 2021Quote: The Fab fragment of the ch14D5 antibody was prepared from the antibody using Immobilized Papain-agarose resin (Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2021Quote: The reaction products of anti-c-Myc Fab labelled with the CF 568 dye were purified using Size exclusion chromatography (SEC-1 MAbPac, Thermofisher) (Supplementary Fig ...
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bioRxiv - Biochemistry 2022Quote: ... were transfected with the appropriate light chain and heavy chain encoding plasmids for each Fab following the ExpiFectamine CHO Transfection Kit (ThermoFisher) high titer protocol ...
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bioRxiv - Biophysics 2022Quote: ... were transfected with the appropriate light chain and heavy chain encoding plasmids for each Fab following the ExpiFectamine CHO Transfection Kit (ThermoFisher) high titer protocol ...
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bioRxiv - Immunology 2019Quote: ... Fabs and IgGs were produced by transient transfection in suspension 293F or adherent HEK 293T cells using Lipofecatamine 2000 (Invitrogen) or polyethylenamine (PEI) ...
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bioRxiv - Microbiology 2022Quote: ... EH3 and EH8 Fab fragments were generated by overnight papain digestion at 37°C using immobilized papain agarose (ThermoFisher Scientific). Fab were separated from Fc and uncleaved IgG by passage over protein A resin followed by size-exclusion chromatography on a Superose 6 10/300 column before being used in SPR binding and X-ray crystallography ...
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bioRxiv - Immunology 2022Quote: The retention times and masses of each of the Fab molecules were retrieved from the generated RAW files using BioPharmaFinder 3.2 (Thermo Scientific). Deconvolution was performed using the ReSpect algorithm between 5 and 57 minutes using 0.1 min sliding windows with 25% offset and a merge tolerance of 30 ppm ...
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bioRxiv - Immunology 2022Quote: The Fab fragments of anti-Muc1 mAbs 14A and 16A were expressed by Bac-to-Bac baculovirus expression system (Invitrogen). The heavy-chain (Hc ...
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bioRxiv - Molecular Biology 2023Quote: The Fab from the CD4i antibody 17b37 was expressed by transient transfection using expression vectors encoding the LC and a C-terminally tagged HC portion of the Fab using the Expi293 expression system (ThermoFisher)7 ...
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bioRxiv - Molecular Biology 2023Quote: ... Fabs were generated from an overnight papain digestion of IgG at 37°C using immobilized papain agarose (Thermo Fisher Scientific). The resulting Fab was separated from Fc and undigested IgG by passage over protein A resin ...
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bioRxiv - Cell Biology 2023Quote: ... The primary antibody was detected by a Fab fragment coupled to Alexa Fluor 647 (Thermo Fisher, A21246; dilution 1:500). The sample was then covered in imaging buffer (27 ...
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bioRxiv - Bioengineering 2024Quote: WT IL-10 and Fab-IL-10 constructs were fluorescently labeled using Alexa Fluor 647 N-hydroxysuccinimide (NHS) ester (Invitrogen), and unreacted dye was removed with a Zeba Spin Desalting Column (7K MWCO ...
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bioRxiv - Molecular Biology 2021Quote: Fab preparation was performed using the same monoclonal antibodies used in ChIP experiments and the Pierce Mouse IgG1 Fab and F(ab’)2 Preparation Kit (Thermo Scientific), as described before27 ...
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bioRxiv - Immunology 2021Quote: ... We generatead F(ab’)2 fragments using Pierce™ Mouse IgG1 Fab and F(ab’)2 Preparation Kit (ThermoFisher Scientific #44980). ROS production upon stimulation with or without 10 μM peptide S100A6-1 coated to a Microfluor® 2 white-bottom 96-well plate (ThermoFisher ...
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bioRxiv - Immunology 2020Quote: ... the 5D5Δg Fab was produced by site-directed mutagenesis of the mAb 5D5 VH region using Accuprime Pfx Supermix (Thermo Fisher Scientific). 5D5Δg Fab was expressed in HEK293F cells and purified by chromatography as described above.
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bioRxiv - Biochemistry 2021Quote: ... Serum Fab concentration was measured 7 days post HDD by ELISA using a goat anti-kappa light chain antibody (ThermoFisher Scientific).
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bioRxiv - Immunology 2021Quote: The Fab fragments of Clone 2 and Clone 6 were generated from full length IgGs of Clone 2 and Clone 6 using a commercial PierceTM Fab Preparation Kit (Thermo Fisher). All procedures were performed following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... TOMM22 was revealed with a species-specific Fab’ secondary antibody coupled to Alexa 647 (Thermo Fisher Scientific, A-21237 or A-21246). TOMM20 and PMPCB were revealed with either secondary antibody depending on the co-stained protein ...
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bioRxiv - Immunology 2022Quote: ... and anti- TCRα (clone H28-710 or H28) mAbs were obtained by digesting commercial full-length mAbs using Fab preparation kit (Thermo Fisher 44685). H57 scFv was a generous gift from Johannes Huppa of the Medical University of Vienna (10).
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bioRxiv - Bioengineering 2020Quote: ... Pro + using an optimized autoinduction media and purified by protein A affinity chromatography similarly to Fabs.30 VH-Fc were expressed in Expi293 BirA cells using transient transfection (Expifectamine, Thermo Fisher Scientific). 4 days after transfection ...
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bioRxiv - Microbiology 2020Quote: ... Fabs were generated by Papain cleavage and purified by the use of the Pierce Fab Preparation Kit according to the manufacturers’ instructions (Thermo Scientific - #44985). The purity of the Fabs was confirmed by SDS-PAGE.
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bioRxiv - Biophysics 2022Quote: ... The supernatant containing the secreted Fab was isolated by centrifugation (4000xg for 20 minutes) and applied to 1-2mL of IgG-CH1 affinity resin (Thermo Fisher Scientific) pre-equilibrated with 1xHBS ...
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bioRxiv - Biophysics 2022Quote: ... Suitable grids and grid regions of hSERINC3-Fab samples were identified by screening atlas images on the UVa MEMC Titan Krios (Thermo Fisher Scientific) using the Falcon 3EC detector ...
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bioRxiv - Cell Biology 2023Quote: ... the disulfide bond linking the two Fab fragments of F(ab’)2 goat anti-mouse Ig(G+M) was reduced using 2-mercaptoethylamine HCL (Thermo Fisher Scientific) and biotinylated by using maleimide-PEG2-biotin (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: The sandwich ELISA assay was performed with 4 µg/ml coated F(ab’)2 fragments that were generated from full-size antibodies using the Pierce Mouse IgG1 Fab and F(ab’)2 Preparation Kit (Thermo Fisher, 44980) according to the manufacturer instructions ...
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bioRxiv - Immunology 2023Quote: Fabs used for ns-EM were purified as follows: expressed IgGs were concentrated and digested using the Pierce™ Fab Preparation Kit (Thermo Fisher 44985) following manufacturer instructions ...
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bioRxiv - Cell Biology 2021Quote: ... VDAC1 or COX2 were revealed by a species-specific Fab’ secondary antibody coupled to Alexa 555 (Thermo Fisher Scientific, A-21430 or A-21425). TOMM22 was revealed with a species-specific Fab’ secondary antibody coupled to Alexa 647 (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... Fluorescently labeled Fabs were made by conjugating with either Alexa Fluor 488 or Alexa Fluor 647 NHS Ester (Thermo Fisher Scientific, A20000 or A20006) to lysine side chains in PBS and molar ratio of dye to Fab was controlled to be in the range of 1 ∼ 2.
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bioRxiv - Immunology 2022Quote: ... genes encoding single chain (sc) Fab and scFc linked to human apoferritin light chain monomers were synthesized and cloned by Geneart (Thermo Fisher Scientific, Waltham, MA) into the pcDNA3.4 expression vector ...
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bioRxiv - Immunology 2022Quote: ... M8a-34 Fab or HSW-1 Fab and SARS-CoV-2 Omicron BA.1 spike 6P with M8a-31 Fab were collected using SerialEM automated data collection software (Mastronarde, 2005) on a 300 keV Titan Krios (Thermo Fisher Scientific, Waltham, MA) cryo-electron microscope equipped with a K3 direct electron detector camera (Gatan ...
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bioRxiv - Immunology 2023Quote: ... The fragment antigen-binding (Fab) part was separated from undigested IgG and the crystallizable fragment (Fc) using an Immobilized Protein A column (Thermo Fisher Scientific, No. 20356). The flow through containing the Fab was concentrated through 10 kDa Amicon centrifugal filter units (EMD Millipore ...
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bioRxiv - Biochemistry 2024Quote: ... and the bound NTCP-Fab complexes were incubated with a secondary HRP-conjugated Pierce recombinant protein L (Thermofisher, 1:5000 dilution in ELISA buffer) for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... rabbit (goat anti-rabbit IgG, Invitrogen), sheep (rabbit anti-sheep IgG ...
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bioRxiv - Biochemistry 2023Quote: ... and the bound target-Fab complexes were incubated with an HRP-conjugated Pierce recombinant protein L (cat: 32420, Thermofisher, 1:5000 dilution in ELISA buffer) for 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-GFP rabbit (A11122, Invitrogen, CA, USA), chicken anti-GFP (AB16901 ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubating primary antibodies at 1:1,000 dilution (rabbit anti-LHX2, Abcam, ab184377; rabbit anti-NFIA, Sigma, HPA006111; rabbit anti-SOX9, EMDMillipore, ab5535; rabbit anti-NPAS3, ThermoFisher, PA520365) for overnight at 4 °C ...
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bioRxiv - Neuroscience 2019Quote: ... and rabbit (1:500 goat anti-rabbit, ThermoFisher A11012) conjugated with two-photon-excitable fluorophores (488nm and 594nm ...
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bioRxiv - Microbiology 2024Quote: ... or rabbit (Donkey anti-rabbit AF594, Invitrogen, Cat# A11012). Cells were washed in 1x PBS ...
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bioRxiv - Immunology 2020Quote: ... Rabbit anti-Fezf2 and rabbit anti-involucrin were revealed with Cy3-conjugated anti-rabbit IgG (Invitrogen) and biotinylated anti-TPA was revealed with Alexa Fluor 488-conjugated streptavidin (Invitrogen) ...
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bioRxiv - Genetics 2023Quote: ... goat α rabbit Cy3 or donkey α rabbit Alexa488 (ThermoFisher). Neuronal membranes were visualized with 1:100 goat α HRP-Alexa 647 (Jackson ImmunoResearch ...
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bioRxiv - Cancer Biology 2023Quote: ... and anti-rabbit secondary antibody (Thermofisher anti-rabbit IgG AF633). Images were acquired using the Thunder Imager Tissue (Leica ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit-antiespin (Invitrogen PA5-55941 ...