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Citations for Thermo Fisher :
151 - 200 of 10000+ citations for FSTL1 Mouse HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: HEK-293-loxP-GFP-RFP cells (GeneTarget Inc) were maintained in Dulbecco’s Modified Eagle Medium (DMEM; Gibco), and supplemented with 10% FBS and 200 units/ml penicillin and streptomycin.
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bioRxiv - Bioengineering 2024Quote: HEK 293 T cells were transfected with DNA plasmids using Lipofectamine™ 3000 Transfection Reagent (ThermoFisher Scientific) per manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2019Quote: Lung tissues from Fstl1 mutant and control mice were collected and homogenized in Trizol (Ambion). RNA extraction and reverse transcription was performed following standard procedures (RevertAid First Strand cDNA Synthesis Kit ...
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bioRxiv - Microbiology 2022Quote: ... Mouse-anti-His (HIS.H8) antibody (Invitrogen) (for eGFP/His-tagged NP protein co-immunoprecipitation ...
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bioRxiv - Biochemistry 2021Quote: ... His (1:1000, Invitrogen, mouse monoclonal), Flag M2(1:1000 ...
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bioRxiv - Neuroscience 2021Quote: HEK 293 cells (ATCC ref CRL-1573) grown on coverslips were transfected using Lipofectamine 2000 (Invitrogen 11668-019) and 1-2 μg of either pULTRA EGFP or pULTRA EGFP T2A C3 Ms (see above ...
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bioRxiv - Biochemistry 2020Quote: ... The expression plasmids were purified and transformed into HEK cells (FreeStyle™ 293-F cell line, Life Technologies) as previously described(Moremen et al ...
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bioRxiv - Biophysics 2021Quote: ... In brief, 2•106 HEK-293 cells (DSMZ, Germany) were transfected in Dulbecco’s modified Eagle’s medium (Life Technologies) containing 10 % fetal bovine serum (Life Technologies) ...
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bioRxiv - Cell Biology 2021Quote: DF-1 chicken fibroblasts and human HEK 293 or 293T cells were grown in Dulbecco’s modified Eagle’s Medium (DMEM) (Invitrogen) supplemented with 10% fetal bovine serum (Biologos) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... stable Nluc-rat A3R expressing HEK 293 cell were selected using 600 μg/mL Geneticin (Thermo Fisher Scientific) whereby the media was changed every two days ...
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bioRxiv - Molecular Biology 2022Quote: HEK 293 T (ATCC CRL-3216) cell line were cultured in DMEM high glucose media (Thermo Fisher, 11965092) containing 10% heat-inactivated fetal bovine serum (Thermo Fisher ...
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bioRxiv - Cell Biology 2020Quote: ... was used to transfect HEK-293 cells at 70-90% confluency using Lipofectamine 3000 reagent (Thermo Fisher Scientific) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: DF-1 chicken fibroblasts and human HEK 293 or 293T cells were grown in Dulbecco’s modified Eagle’s Medium (DMEM) (Invitrogen) supplemented with 10% fetal bovine serum (Biologos) ...
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bioRxiv - Microbiology 2020Quote: ... The pAd-hACE2 was linearized with PacI restriction enzyme before transfection into T-Rex 293 HEK cells (Invitrogen) to generate HuAdv5-hACE2 ...
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bioRxiv - Physiology 2022Quote: HEK-293 cells were cultured in 6 well plate to 60% confluence and transfected using lipofectamine LTX (Invitrogen) using 2.5 ug of pCMV6-XL5 or pCMV6-XL5-AVP (Origene ...
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bioRxiv - Neuroscience 2023Quote: Lentivirus was prepared by co-transfection of a confluent 15 cm plate of HEK 293-FT cells (Invitrogen) with 8 μg of transfer vector ...
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bioRxiv - Cell Biology 2023Quote: ... and HEK 293 Flp-In™ T-REx™ 293 cells stably expressing Cas9 (referred to throughout the paper as HEK-293) were cultured in Dulbecco’s modified Eagle’s medium Glutamax (DMEM; Gibco) supplemented with 10% foetal calf serum (FCS ...
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bioRxiv - Molecular Biology 2024Quote: Human Embryonic Kidney 293 (HEK) cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Life Technologies-Invitrogen, USA), supplemented with 10% Fetal Bovine Serum Gold (FBS ...
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bioRxiv - Biophysics 2024Quote: Human embryonic kidney (HEK) 293 cell lines were maintained in T-25 flasks using TrypLE Express Enzyme (Gibco) and DMEM with L-Glutamine ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... While HEK-293 and HEK/R-GECO1/PH(Akt)-Venus were cultured in the Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen), С6 and CHO/5-HT2C were maintained in the F12 medium (Invitrogen) ...
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bioRxiv - Molecular Biology 2024Quote: Human Embryonic Kidney 293 (HEK) cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Life Technologies-Invitrogen, USA), supplemented with 10% Fetal Bovine Serum Gold (FBS ...
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bioRxiv - Genomics 2020Quote: ... mouse fibroblast NIH-3T3 L929 cells and human embryonic kidney HEK 293 cells were grown in DMEM medium (Dulbecco’s Modified Eagle Medium, Gibco) supplemented with sterile-filtered 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2021Quote: ... dsiRNAs targeting Rpp30 (10 nM) were transfected into HEK 293 cells using Lipofectamine 3000 reagent according to the manufacturer’s instructions (Invitrogen). Target are available on www.idtdna.com ...
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bioRxiv - Neuroscience 2020Quote: Human embryonic kidney (HEK)-293 cells (ATCC, CRL-3216) were cultured in Dulbecco’s modified Eagle’s medium (Gibco, 11965-092) supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2021Quote: ... 0.6 µg KV3.1b plasmid and 0.1 µg eGPF were transfected into HEK-293 cells using Lipofectamine 2000 (Invitrogen Corporation), and recordings were performed after 3 days of incubation at 37 °C ...
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bioRxiv - Biophysics 2020Quote: ... HEK-293 cells were transiently transfected with NaChBac WT or T220A cDNA using Lipofectamine™ 2000 transfection reagent (Invitrogen) and seeded onto 12 mm circular glass coverslips 24 hours prior to patch clamp recording ...
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bioRxiv - Neuroscience 2020Quote: ... expression vector plasmids encoding opsins or hrGFP were transfected into HEK 293 cells using Lipofectamine 2000 (Thermo Fisher Scientific). The cells were washed with phosphate-buffered saline (PBS ...
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bioRxiv - Physiology 2020Quote: ... 24 hours post-transfection, HEK 293 cell were harvested, resuspended in reduced serum media (MEM, NEAA (Thermo Fisher Scientific) supplemented with 1% L-glutamine (2 mM final ...
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bioRxiv - Synthetic Biology 2020Quote: ... human embryonic kidney 293 (HEK 293)(ATCC) cells were seeded in a 6-well plate at 0.3×106 cells/well in DMEM (ThermoFisher) supplemented with 10% FBS and 1% penicillin-streptomycin (Gibco ...
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bioRxiv - Neuroscience 2019Quote: Human embryonic kidney 293 (HEK 293) cells were cultured on 100 mm culture dishes in Dulbecco’s Modified Eagle’s medium (DMEM-GlutaMAX, Life Technologies) supplemented with 10% Foetal Calf Serum (FCS ...
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bioRxiv - Biochemistry 2021Quote: ... Plasmids (3 µg) were introduced into HEK 293 cells (1×106) by electroporation with Neon electroporator (Thermo Fisher Scientific) according to the manufacturers protocol ...
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bioRxiv - Synthetic Biology 2021Quote: Flp-In™ T-REx™ Human Embryonic Kidney (HEK) 293 cells were purchased from Thermo Scientific (Catlog# R78007). Cells were cultured in a humidity-controlled incubator under standard culture conditions (37°C with 5% CO2 ...
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bioRxiv - Genetics 2020Quote: ... Human embryonic kidney cells (HEK-293, ATCC) were grown in Dulbecco’s modified Eagle medium supplemented with 10% fetal bovine serum (Gibco). Cell lines were regularly screened for mycoplasma contamination by DAPI staining ...
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bioRxiv - Biophysics 2022Quote: ... HEK 293 cells and COS-7 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Thermo Fisher Scientific, USA) supplemented with glucose (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... HEK-293 cells were purchased from ATCC and cultured in 10% (v/v) FBS (Gibco, Cat. No. 10270-106) and 100U mL-1 penicillin and 100µg mL-1 streptomycin (Gibco ...
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bioRxiv - Biochemistry 2023Quote: ... HEK-293 cells were purchased from ATCC and maintained in 10% (v/v) FBS (Gibco, Cat. no. 10270-106) and 100U ml-1 penicillin and 100 µg ml-1 streptomycin (Gibco ...
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bioRxiv - Molecular Biology 2024Quote: ... in a pcDNA5/FRT/TO/n2SC or pcDNA5/FRT/TO/c2SC vector with 0.9 µg of pOG44 plasmid into HEK 293 Flp-In T-REx cells (Invitrogen). Cells were selected for 2 weeks in DMEM containing 100 µg/mL hygromycin and 15 µg/mL blasticidin ...
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bioRxiv - Biochemistry 2024Quote: ... HEK-293 cells were purchased from ATCC and maintained in 10% (v/v) FBS (Gibco, Cat. no. 10270-106) and 100 U ml-1 penicillin and 100 µg ml-1 streptomycin (Gibco ...
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bioRxiv - Developmental Biology 2023Quote: HEK-293 (ATCC CRL-1573) was maintained in DMEM supplemented with 10% fetal bovine serum and penicillin/streptomycin (Invitrogen). Cells were cultured according to the manufacturer’s instructions (ATCC) ...
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bioRxiv - Microbiology 2021Quote: ... mouse anti-His (MA1-21315, Thermo Scientific), mouse anti-β-actin HRP (Santa Cruz Biotechnologies ...
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bioRxiv - Biochemistry 2021Quote: ... mouse anti-His Tag (Invitrogen MA1-21315). Secondary antibody used are ...
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bioRxiv - Microbiology 2022Quote: ... mouse monoclonal anti-6x-His (Thermo Fisher) diluted 1:1,000 ...
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bioRxiv - Bioengineering 2020Quote: ... HEK 293T cells were cultured in DMEM (HyClone) supplemented with 10% heat-inactivated FBS (HI-FBS; ThermoFisher). CHLA-255-Luc-EGFP cells were cultured in IMDM (ThermoFisher ...
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bioRxiv - Cancer Biology 2021Quote: ... was then gently mixed with 20 parts (v/v) of HEK-293 cells (ATCC) suspended at a density of 2 x 105 cells/mL in DMEM (Gibco) + 10% FBS (Seradigm/VWR) ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... HEK-293 cells were transferred with a density of 12000 cells per well in white 96-well plates (Thermo Scientific™ Nunc™ F96 MicroWell™ ...
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bioRxiv - Bioengineering 2021Quote: All experiments used a previously described research cell bank of T-REx-293 human embryonic kidney (HEK) cells stably expressing the tetracycline repressor protein (ThermoFisher) (4) ...
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bioRxiv - Biochemistry 2020Quote: ... HEK 293T and T-Rex 293 cells were grown in Dulbecco’s modified Eagles medium (DMEM, Thermo Fisher scientific, MA, USA) containing 4.5 mg/ml glucose ...
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bioRxiv - Neuroscience 2020Quote: ... Lentiviruses were produced by transfecting HEK 293 cells with lentiviral vectors together with packaging vectors (3rd generation) using Lipofectamine 3000 (Thermofisher). Viral supernatant was collected 24hr and 48hr post-transfection ...
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bioRxiv - Genetics 2022Quote: HEK 293 cells were cultured in medium consisting of high glucose DMEM (Hyclone) supplemented with 10% fetal bovine serum (Gibco) and 1× penicillin-streptomycin (Gibco) ...
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bioRxiv - Microbiology 2020Quote: ... The pSAd36-S and pSAd-control plasmids were linearized with PacI restriction enzyme to liberate viral genomes for transfection into T-Rex 293-HEK cells (Invitrogen). The rescued replication-incompetent ChAd-SARS-CoV-2-S and ChAd-Control vectors were scaled up in 293 cells and purified by CsCl density-gradient ultracentrifugation ...