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Citations for Thermo Fisher :
501 - 550 of 10000+ citations for Ephrin type A receptor 8 EphA8 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: HEK293 cells were cultured in Dulbecco’s modified Eagle’s medium (Gibco, France) supplemented with 10% fetal calf serum (Eurobio ...
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bioRxiv - Evolutionary Biology 2023Quote: ... HEK293 cells were washed with Opti-MEM Reduced Serum Media (Gibco) and incubated with transfection mix containing 1 µg total of the indicated plasmid DNA and 3 µL Lipofectamine 3000 Transfection Reagent (Invitrogen ...
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bioRxiv - Physiology 2023Quote: ... HEK293 cells were washed with Opti-MEM Reduced Serum Media (Gibco) and transfected using lipofectamine 2000 (Invitrogen/Life Technologies Cat #11668019 ...
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bioRxiv - Biochemistry 2023Quote: ... After transfection the Flp-In TRex HEK293 (Flp-In-293, Invitrogen) cells were selected in the presence of Hygromycin B (100 µg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 and C166 cells were maintained in DMEM (high glucose, Gibco) supplemented with 10% FBS (Giboco) ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293-FT (RRID:CVCL_6911) were culture similarly but in DMEM medium (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: HEK293-T cells were cultured in DMEM medium (#31966-021, Gibco) supplemented with 10% fetal bovine serum (#10270-106 ...
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bioRxiv - Neuroscience 2023Quote: HEK293 cells were grown in Dulbecco’s Modified Eagle Medium (DMEM, ThermoFisher) containing 10% fetal calf serum and 0.5% penicillin/streptomycin ...
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bioRxiv - Cell Biology 2019Quote: ... mouse monoclonal anti-transferrin receptor (1:1000, ThermoFisher, #13-6800), Membranes were stained with the relevant secondary antibodies in 3% milk in TBST ...
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bioRxiv - Cell Biology 2019Quote: ... mouse monoclonal anti-transferrin receptor (1:100, ThermoFisher, #13-6800), mouse monoclonal anti-syntaxin 6 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... then Fc receptors blocked with normal mouse serum (Thermo Fisher) and TruStain FcX αmouse CD16/32 (Biolegend ...
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bioRxiv - Cancer Biology 2019Quote: ... Progesterone Receptor (clone hPRa7, #MA5-12658, Thermo Scientific, Loughborough, UK), p63 (clone BC4A4 ...
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bioRxiv - Cell Biology 2020Quote: ... anti-Transferrin receptor (clone H68.4, ThermoFisher Scientific, cat# 13-6800), anti-α-tubulin mouse monoclonal (Sigma-Aldrich ...
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bioRxiv - Physiology 2022Quote: ... mouse anti-Transferrin receptor (1:1000, 13-6800, Life Technologies). The membranes were subsequently washed 4 times in TBS-T for 10 min before incubating in the appropriate secondary antibody anti-rabbit HRP (1:5000 ...
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bioRxiv - Molecular Biology 2022Quote: ... mouse anti-Transferrin receptor (1:1000, 13-6800, Life Technologies). The following day ...
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bioRxiv - Cell Biology 2022Quote: ... transferrin receptor (Clone MEM-189, MA1-19300, ThermoFisher/Zymed; RRID:AB_2536952); and α-tubulin DM1A (Sigma-Aldrich T9026 ...
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bioRxiv - Immunology 2023Quote: ... for murine and Fc Receptor Binding Inhibitor Polyclonal Antibody (Invitrogen) for human samples and subsequently stained with live/dead cell exclusion dye (Zombie Dyes ...
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bioRxiv - Cell Biology 2023Quote: ... 2 μM DDR1 (Fisher Scientific, discoidin domain receptor 1 inhibitor). Time lapse images were acquired every 10 minutes for 24 hours.
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bioRxiv - Neuroscience 2023Quote: ... and allophycocyanin-conjugated mouse anti-glucocorticoid receptor (GR-APC; Invitrogen; cat# 17-6189-82 ...
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bioRxiv - Cancer Biology 2023Quote: ... progesterone receptor (rabbit monoclonal SP2, Thermo Scientific, RM-9102-R7), NeuN (mouse monoclonal A60 ...
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bioRxiv - Neuroscience 2024Quote: ... serotonin receptor 2C (5-HTR2C; Invitrogen, Waltham, MA; 1:1000). Membranes were washed three times in 0.1% Tween-20 in 1X TBS (TBST ...
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bioRxiv - Bioengineering 2023Quote: ... / type-I collagen (Gibco™ collagen type I ...
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bioRxiv - Cell Biology 2023Quote: ... type I (ThermoFisher, #A1048301); Fibronectin ...
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bioRxiv - Microbiology 2020Quote: Human ileocecal adenocarcinoma HCT-8 cells (ATCC) and THP-1 cells (ATCC) were maintained in Advanced RPMI 1640 (ThermoFisher Scientific) supplemented with 10% fetal bovine serum and 1X penicillin-streptomycin (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... IL-8 concentrations in medium samples from the basal compartment were measured using the IL-8 Human Uncoated ELISA Kit (88-8086-88, Invitrogen). Assays were performed in Nunc MaxiSorp (tm ...
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Dynamic chromatin organization and regulatory interactions in human endothelial cell differentiationbioRxiv - Developmental Biology 2022Quote: ... from the RUES2 line (RUESe002-A; WiCell) were maintained on recombinant human Laminin-521 matrix (rhLaminin521; Biolamina) in Essential 8 (E8) media (ThermoFisher) at a density of 0.5 µg/cm2 ...
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bioRxiv - Cell Biology 2022Quote: ... with Essential 8 Supplement (1X) in culture vessels coated with 5mg/ml vitronectin (Recombinant Human Protein; Life Technologies, London, UK). Spontaneous differentiation medium was Knockout DMEM ...
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bioRxiv - Genomics 2019Quote: ... hiPSCs were cultured on plates pre-coated with 0.5 μg/cm2 recombinant human Laminin-521 matrix (rhLaminin521; Biolamina) with daily changes of antibiotic-free Essential 8 (E8) media (ThermoFisher). Cells were passaged as small clumps with Versene (ThermoFisher) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... IPSCs were maintained on human Laminin-521 (BioLamina, Sundbyger, Sweden) coating in essential 8 (E8) medium (DMEM/F12 supplemented with 15 mM Hepes (Gibco/Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... Treg cells were stimulated for 7-8 days with Dynabeads™ Human T-Activator CD3/CD28 (1:1 beads:Treg cell ratio, Gibco), and cultured in X-VIVO 15 with Gentamicin ...
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Cell cycle plasticity underlies fractional resistance to palbociclib in ER+/HER2- breast tumor cellsbioRxiv - Cancer Biology 2023Quote: ... T47D (ATCC HTB-133) or primary cells from human tumors were fixed by adding 8% PFA (Thermo Scientific cat#28908) directly to the samples (1:1 v/v with media ...
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bioRxiv - Microbiology 2023Quote: ... Human alveolar A549 cells were seeded in an 8 well chamber slide (Lab-Tek II Chamber Slide w/ Cover, NUNC), and once confluent ...
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bioRxiv - Cancer Biology 2020Quote: ... His-tagged GOT2 protein was immunoprecipitated using the His-tag isolation and pull-down Dynabeads system (Thermo Fisher) using the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and further ligated into pcDNA 3.1 V5/His TOPO vector using pcDNA 3.1 V5/His TOPO TA cloning kit (Invitrogen). The ACRD etoll was then transferred to pGADT7-AD vector using Nde1 and BamH1 sites ...
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bioRxiv - Cell Biology 2022Quote: ... The His SNX32ΔC protein bound to the lipids was detected by anti-His antibody [1:10,000 (Thermo Scientific)]
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bioRxiv - Molecular Biology 2023Quote: ... ceSMSγ and ceSMSr cDNAs were cloned into yeast expression vector pYES2.1/V5-His-TOPO and mammalian expression vector pcDNA3.1/V5-His-TOPO (Invitrogen) as described previously(18).For expression studies in Drosophila S2 cells ...
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bioRxiv - Microbiology 2023Quote: ... whereas His-tagged EF-P was detected with primary monoclonal antibodies against the His-Tag (1:10,000; AB_2536841, Invitrogen). Membranes were incubated with the secondary antibodies conjugated with a fluorophore (1:20,000 in TBS ...
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Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: Human wild-type (WT) alpha-synuclein was expressed in Escherichia coli One Shot® BL21 STAR™ (DE3) (Invitrogen, Thermo Fisher Scientific) cells using plasmid pT7-7 and purified using ion-exchange on a HiPrep Q FF 16/10 anion exchange column (GE Healthcare ...
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Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: Human wild-type (WT) alpha-synuclein was expressed in Escherichia coli One Shot® BL21 STAR™ (DE3) (Invitrogen, Thermo Fisher Scientific) cells using plasmid pT7-7 and purified using ion-exchange on a HiPrep Q FF 16/10 anion exchange column (GE Healthcare ...
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bioRxiv - Molecular Biology 2022Quote: Human VAT and SAT biopsies (30-50g) were minced and digested with 3mg/mL type II collagenase solution (ThermoFisher Scientific Inc./Gibco) in HBSS containing calcium chloride ...
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bioRxiv - Neuroscience 2022Quote: ... were placed in co-culture with astrocytes 24 h following purification and both cell types were maintained in human ESFM supplemented with B-27 (Thermo Fisher Scientific). Co-cultures were maintained for 24 h prior to experiments.
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bioRxiv - Bioengineering 2023Quote: ... To create SiRPα-Fc fusion soluble proteins the cDNA sequence of the full extracellular domain (aa 27-371) of human wild-type allele 1 SiRPα (wtSiRPα) cDNA (Uniprot: P78324) was codon-optimized and synthesized by GeneArt (Thermo Fisher Scientific), fused with the cDNA sequence of the human hinge and IgG1 heavy chain Fc region (pFUSE-hIgG1-Fc1 ...
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bioRxiv - Immunology 2023Quote: The coding sequence of wild-type human CXCR4 was synthesized by General Biol (Chuzhou, China) and was cloned into the pFastBac transfer plasmid (Invitrogen, Carlsbad, CA). Heat-stabilized BRIL ...
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bioRxiv - Genetics 2021Quote: ... 12 µL Hi-Di Formamide (Applied Biosystems) and 0.3 µL MapMarker 500 labelled with DY-632 (Eurogentec) ...
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bioRxiv - Microbiology 2019Quote: ... we used Dynabeads™ His-Tag (Invitrogen) for Ni-affinity purification ...
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bioRxiv - Bioengineering 2022Quote: 6x-His tag antibodies (Invitrogen, MA1-21315) were coated at 2 µg/mL in PBS onto 96-well half- area high binding plates (Corning ...
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bioRxiv - Immunology 2021Quote: ... blot using an anti-His Ab (ThermoFisher). MSP-142 and AMA-1 were biotinylated and tetramerized with streptavidin-PE (SA-PE ...
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bioRxiv - Microbiology 2021Quote: ... eluted in Hi-Di formamide (Applied Biosystems) with ROX-350 ladder ...
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bioRxiv - Biochemistry 2021Quote: ... anti-His (MA1-21315, Invitrogen, 1:1,000). Proteins separated by SDS-PAGE were detected following the manufacturer’s guidelines (GE Healthcare ECL Western Blotting detection system ...
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bioRxiv - Microbiology 2020Quote: Hi-sensitivity Qubit DNA kit (Thermo Fisher) was used to measure the DNA concentration directly from the lysate ...