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Citations for Thermo Fisher :
551 - 600 of 10000+ citations for En 1948 4 Who Dioxin Like Pcb "Starter Kit" since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: Cells were stained for 30 min at 4 ºC with either LIVE/DEAD fixable violet or yellow dead stain Kit (Life Technologies), with empirically titrated concentrations of the following antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... The lysates were centrifuged at 14,000 x g at 4 °C and RNA concentration was measured using Qubit BR RNA Assay Kits (ThermoFisher Scientific, Q10210). For IP ...
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bioRxiv - Cancer Biology 2023Quote: ... fixed in 4% PFA and EdU-labeling was performed according to manufacturer instructions (Click-iT EdU Cell Proliferation Kit for Imaging, Thermo Fisher).
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bioRxiv - Immunology 2023Quote: ... cells were fixed for 40 min at 4°C using the eBioscience™ Foxp3/Transcription Factor Fixation kit (Thermo Fisher Scientific) and permeabilized using the kit’s permeabilization buffer ...
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bioRxiv - Neuroscience 2023Quote: Calcium flux was measured in MNs seeded in a 96 well plate using the Fluo-4 NW Calcium Assay Kit (ThermoFisher, #F36206) per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Extracted DNA samples were quantified with Qubit™ dsDNA HS Assay Kit using a Qubit 4 fluorometer (Invitrogen, Waltham, MA, USA).
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bioRxiv - Pharmacology and Toxicology 2023Quote: Thirty µg of cytosolic or 10µg of mitochondrial proteins from each sample were separated on NuPAGE® Novex® Bis-Tris 4–12% gels kit (Invitrogen) and transferred to PVDF membranes (Trans-blot® Turbo™ Transfer System ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was collected after centrifuging at 13,000 RPM (10 mins, 4°C) and total protein was estimated by BCA (Pierce BCA Protein Assay Kit, ThermoFisher Scientific, 23225). The supernatant was mixed with 1X Laemmli buffer and boiled at 95°C for 10 minutes ...
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bioRxiv - Microbiology 2023Quote: Cells were stained for 30 min at 4 °C with either LIVE/DEAD fixable violet or yellow dead stain Kit (Life Technologies), with empirically titrated concentrations of the following antibodies ...
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ALS iPSC-derived microglia and motor neurons respond to astrocyte-targeted IL-10 and CCL2 modulationbioRxiv - Neuroscience 2023Quote: Calcium flux was measured in MNs seeded in a 96 well plate using the Fluo-4 NW Calcium Assay Kit (ThermoFisher, #F36206) per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were centrifuged at 20,000 x g for 10 min at 4 °C and protein concentrations were determined using Pierce BCA Protein Assay Kit (ThermoFisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration was determined using a BCA protein assay kit (Pierce) and 15-20 μg of protein was separated on a 4-12% NuPAGE Bis-Tris gel (Life Technologies) followed by transfer to PVDF membranes ...
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bioRxiv - Genetics 2023Quote: ... 600 ng of purified DNA template was in vitro transcribed for 4 h at 37°C using MEGAshortscript T7 Transcription Kit (Invitrogen, AM1354). sgRNA was purified using MEGAclear Transcription Clean-Up Kit (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... The soluble fractions of cell lysates were isolated by centrifugation at 20,000 g at 4 °C for 10 min and quantified by a BCA kit (Thermo Fisher Scientific). Protein samples were denatured with the addition of 6 × SDS sampling buffer and incubated at 95 °C for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... The antibody-antigen complexes were incubated overnight at 4°C and eluted following Pierce Co-Immunoprecipitation (Co-IP) Kit (Thermo Fisher). Samples were then separated by gel electrophoresis followed by immunoblotting ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were centrifuged at 13,000 rpm for 10 min at 4°C and the protein concentrations were quantified using Pierce BCA Protein Assay Kit (Thermo Fisher). Equal amounts of protein were separated on 8% SDS-PAGE and then transferred onto nitrocellulose membranes (Thermo Fisher) ...
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bioRxiv - Microbiology 2023Quote: ... RNA final concentration was measured with a Qubit 4 Fluorometer using the HS RNA kit (Thermo Fisher Scientific, Waltham, MA, USA), and RIN numbers were obtained using the 4200 TapeStation System (Agilent ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The quality of DNA was checked using 1.5% agarose gel electrophoresis and concentration was measured by Qubit 4 fluorometer with 1X dsDNA HS Assay Kit (Thermo Fisher Scientific). As the basis for ddRAD library protocol development ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we quantified the DNA concentration with a Qubit 4 fluorometer using a Broad Range (BR) or High Sensitivity (HS) assay kit (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2024Quote: Cells were fixed using 4% PFA and RNA was extracted from the fixed cells using the PureLink FFPE RNA Isolation Kit (ThermoFisher K156002), and treated with Ambion DNase I (ThermoFisher AM2222 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were lysed at 4°C with RIPA buffer and protein concentration was determined with the Micro kit BCA Protein Assay (Thermo Scientific, Courtaboeuf ...
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bioRxiv - Molecular Biology 2024Quote: ... The complex was incubated for 30 minutes at 4 ℃ before adding 10X CMC CHAPS (VitroEase™ Buffer Screening Kit, ThermoFisher Scientific) to a final concentration of 0.25X CMC ...
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bioRxiv - Molecular Biology 2024Quote: ... The complex was incubated for 30 minutes at 4 ℃ before adding 10X CMC CHAPS (VitroEase™ Buffer Screening Kit, ThermoFisher Scientific) to a final concentration of 0.5X CMC ...
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bioRxiv - Cancer Biology 2023Quote: ... fixed in 4% PFA and EdU-labeling was performed according to manufacturer instructions (Click-iT EdU Cell Proliferation Kit for Imaging, Thermo Fisher). Images were acquired with a Zeiss LSM700 or LSM880 confocal microscope.
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bioRxiv - Bioengineering 2024Quote: Cell viability was assessed following shear stress application by incubating each sample in a solution containing 2 µM calcein AM and 4 µM ethidium homodimer-1 in PBS (Live/Dead Viability/Cytotoxicity Kit; Thermo Fisher) for 30 minutes at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... The fluorogenic probe 7-diethylamino-3-(4’-maleimidylphenyl)-4-methylcoumarin (abbr. CPM, ThermoFisher, Cat# D346) in 100% DMSO was then added to both quench the enzymatic reaction and react with CoASH to yield the fluorescent CPM-SCoA complex for fluorescence quantification 27 ...
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bioRxiv - Cell Biology 2019Quote: ... Slides were incubated overnight at 4°C with primary Phospho-Smad 2 (ThermoFisher; #4-244G) antibody diluted 1:200 in blocking buffer ...
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bioRxiv - Neuroscience 2023Quote: ... supplements and Fluo-4 acetomethylester (Fluo-4-AM) were from Life Technologies (Carlsbad, CA, USA). Culture plates were from Costar (Tewksbury ...
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bioRxiv - Physiology 2023Quote: Isolated atrial myocytes were stained with 4 µM di-4-ANEPPS for 10 minutes (Invitrogen) for membrane visualisation[3 ...
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bioRxiv - Immunology 2024Quote: ... and recombinant human interleukin 4 (rhIL-4) (300 U/mL; Thermo Fisher Scientific, Waltham, MA). On day 2 and day 5 post-monocyte isolation ...
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bioRxiv - Developmental Biology 2024Quote: ... samples were fixed overnight at 4° C temperature in 4% paraformaldehyde in PBS buffer (Invitrogen) and incubated in 70% ethanol solution after three times’ wash with PBS buffer for paraffin section ...
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bioRxiv - Cancer Biology 2023Quote: MOLT-4 Cells were loaded with the fluorescein Ca2+ probe Fluo-4 AM (Invitrogen # F14217). Cells were incubated for 60 min in RPMI medium and supplemented with 1 µM Fluo-4/AM ...
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bioRxiv - Plant Biology 2019Quote: ... pollinated stigma was incubated in FM™ 4-64 Dye (N-3-Triethylammoniumpropyl-4-6-4-Diethylamino Phenyl Hexatrienyl Pyridinium Dibromide, Life Technologies T3166, 8.23 μM) for five minutes and subsequently washed in 1/2 Murashige and Skoog basal medium containing 10 % (w/v ...
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bioRxiv - Neuroscience 2021Quote: ... After added 24 hours the cells were fixated with 4% paraformaldehyde in PBS for 20mins and then stained using the Click-iT EdU kit and protocol (Invitrogen, Carlsbad, CA) and mounted using Prolong Gold mounting medium with DAPI (Life Technologies) ...
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bioRxiv - Molecular Biology 2021Quote: RNA was isolated from WT and MafAS64F/+ islets (n=4 for males, n=5 for females) using the RNAqueous total RNA isolation kit (Ambion; Thermo Fisher), and then analyzed on an Agilent 2100 Bioanalyzer ...
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bioRxiv - Genomics 2020Quote: ... The DNA was stored at 4°C overnight to facilitate DNA homogenization and then quantified using a Qubit dsDNA HS Assay Kit (Molecular Probes/Life Technologies). The labeled DNA was stained with an intercalating dye and left to stand at room temperature for at least 2 hrs before loading onto a Bionano Chip.
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bioRxiv - Microbiology 2019Quote: Total RNA was isolated from biological triplicates of early and late passage MDCK from T25 flasks that had been seeded with 8 x 105 cells and allowed to become confluent and polarize over 4 days in culture cells using a Ribopure kit (Ambion, Austin, TX). Acquired RNA was precipitated with EtOH and subsequently purified employing columns ...
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bioRxiv - Cell Biology 2019Quote: ... staining was performed in three-dimensional cysts with 2 mM calcein AM and 4 mM ethidium homodimer I (Live/Dead® Viability/Cytotoxicity kit; Life Technologies) for 40 min at 37°C in the dark ...
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bioRxiv - Microbiology 2019Quote: ... Samples were centrifuged at 10,000 x g for 30 min at 4 °C before supernatants were collected and protein content was determined using the BCA Protein Assay Kit (Pierce, Thermo Fisher Scientific) following the manufacturer’s instructions.
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bioRxiv - Genetics 2020Quote: ... Total RNA was extracted from the left side of thorax with gynomorph-like color where siRNA was introduced and from the control region on the right side of the thorax of the same individual (N = 4 each for male and andromorphic female adults) using RNAqueous™-Micro Total RNA Isolation Kit (Thermo Fisher). cDNA was synthesized using SuperScript IV Reverse Transcriptase (Thermo Fisher ...
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bioRxiv - Genomics 2020Quote: ... the following components were added: 4 μl of 5× RT buffer (from the Maxima H Minus Reverse Transcriptase kit, Thermo Fisher Scientific), 1 μl of RNaseOUT™ (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentrations were determined following centrifugation of the lysate at 16,000 x g at 4 °C for 20 minutes using the using a Pierce™ Coomassie (Bradford) Protein Assay Kit (23200, Thermo Scientific) with bovine serum albumin as the standard.
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bioRxiv - Cell Biology 2021Quote: ... Lysates were centrifuged at 14000 rpm for 15 minutes at 4°C and protein concentration determined with a Pierce™ BCA Protein Assay Kit (Life Technologies). High capacity neutravidin beads (Life Technologies ...
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bioRxiv - Plant Biology 2021Quote: ... RNA concentration and quality were assessed by fluorometry and electrophoresis using a Qubit™ 4 and Qubit RNA HS Assay Kit (ThermoFisher Scientific) and a TapeStation (Agilent).
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bioRxiv - Plant Biology 2020Quote: ... RNA quality and concentration were assessed using a NanoDrop™ 1000 spectrophotometer and a Qubit 4 Fluorometer with an RNA HS Assay Kit (ThermoFisher Scientific).
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bioRxiv - Microbiology 2020Quote: ... Two hundred microliters of 2 μM calcein AM/4 μM ethidium homodimer-1 in PBS (Live/Dead® Viability Kit, Invitrogen, USA) were added to the wells and plates incubated for 30 min in the dark ...
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bioRxiv - Cancer Biology 2020Quote: ... cancer cells were fixed in 4% PFA for 20 min at RT and stained according to the Click-iT EdU imaging kit protocol (Invitrogen, California, USA). Stained cancer cells were imaged and quantified using the ImageXpress Micro Confocal microscope (Molecular Devices ...
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bioRxiv - Immunology 2022Quote: ... and amine-reactive viability stained for 30 minutes at 4° C with Live/Dead Fixable Cell Stain Kit (#L34960, Thermo Fisher Scientific). Next ...
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bioRxiv - Pathology 2022Quote: ... Sequencing of OXR1 cDNA from lymphocytes cDNA was prepared from fresh lymphocytes of patient II-4 and control using Maxima™ 1st strand cDNA synthesis kit (Thermo Scientific) and amplified by PCR using Phusion High-Fidelity PCR Master Mix (Thermo Scientific) ...
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bioRxiv - Genetics 2020Quote: ... 4 μL of purified product was cloned into the pCR-4 Blunt TOPO vector using the Zero Blunt TOPO PCR Cloning Kit for Sequencing (ThermoFisher Scientific, USA). To identify larger deletions in the promoter region ...