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Citations for Thermo Fisher :
101 - 150 of 186 citations for Diphtheria Toxin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: Injections of cholera toxin subunit b conjugated to Alexa Fluor 555 (CTB-555) (Thermo Scientific C34776) was injected at 1X 150-200nl in the secondary motor cortex as described above ...
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bioRxiv - Biophysics 2024Quote: The cholera toxin subunit B-Alexa FluorTM 488 and 647 conjugates were obtained from Thermo Fisher Scientific and applied to cultured cells at a concentration of 20 nM for a duration of ∼10 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... The concentration of soluble toxin in the supernatant was quantified using a Nanodrop 2000 (Thermo Fisher Scientist) by measuring the OD at 280 nm and by using the molar extinction coefficient and toxin size (102,930 M-1 cm-1 and 72.349 kDa ...
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bioRxiv - Biophysics 2021Quote: ... and 10 μg/mL Cholera Toxin Subunit B (Recombinant) Alexa Fluor™ 555 Conjugate (CTSB, Fisher Scientific) to label GM1 as the acceptor ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated overnight with primary Cholera Toxin beta polyclonal antibody (rabbit; 1:1000, RRID: AB_779810, Invitrogen) diluted in PBS-T and for approximately 1h30 with donkey antirabbit secondary antibody AlexaFluor647 (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... Alexa Flour 594 Cholera toxin beta subunit conjugate (CTb-594, Invitrogen; 0.5% w/v, 400–500 nl) was injected in dHPC of CD-1 mice ...
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bioRxiv - Biophysics 2023Quote: ... Alexa Fluor 488 conjugated with cholera toxin B subunit (CTxB-Alexa 488) was obtained from Molecular Probes, Life Technologies ...
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bioRxiv - Neuroscience 2023Quote: Intravitreal injections of AAVs (AAV2-Flex-GFP, UNC Vector Core) or cholera toxin B (CTB, Life Technologies) were performed as previously described (Sun et al. ...
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bioRxiv - Neuroscience 2023Quote: For striatal injections of cholera toxin subunit-B (CTB) Alexa fluor-conjugated 488 (Invitrogen Cat. No C34775), unilateral injections were performed into the ipsilateral dorsal striatum to the 6OHDA lesion side (AP +0.5 mm ...
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bioRxiv - Neuroscience 2023Quote: ... 1.5 μl of cholera toxin β subunit (CTB) conjugated with fluorescence Alexa-594 (2 μg/μl, Invitrogen) was injected into the vitreous 2 days before sacrificing the animals ...
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bioRxiv - Neuroscience 2021Quote: Intraocular injection of cholera toxin subunit B (CTB) conjugated to Alexa Fluor 488 or Alexa Fluor 555 (Invitrogen) was performed as described previously (66 ...
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bioRxiv - Neuroscience 2021Quote: ... animals were injected with fluorescent cholera toxin subunit B (CTb-AlexaFluor488, CTb-AlexaFluor594; ThermoFisher, C34775 and C34777, respectively) into AcbSh and AcbC (counterbalanced).
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bioRxiv - Neuroscience 2019Quote: For rats injected with cholera toxin subunit B (CTb) conjugated with Alexa Fluor-488 (CTb-488; ThermoFisher Scientific) in the BNST ...
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bioRxiv - Biophysics 2019Quote: ... cells were incubated in 1:1000 dilution of Cholera Toxin Subunit B (CtxB)-Alexa555 (Invitrogen, 5mg/ml stock) and rinsed 3 times with PBS ...
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bioRxiv - Neuroscience 2021Quote: ... RGC axons were anterogradely labeled by CTB (Cholera Toxin Subunit B) conjugated with Alexa Fluor™ 555 (Invitrogen, C34776) through intravitreal injection 48 hr before sacrifice ...
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bioRxiv - Neuroscience 2021Quote: ... we injected 40nl of cholera toxin subunit B (CTB) conjugated to Alexa Fluor 488 (0.5%) or 647 (0.25%; Life Technologies) into the pulvinar ...
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bioRxiv - Neuroscience 2020Quote: ... A third monkey was injected with a cholera toxin subunit B-Alexa Fluor 647 conjugate (CTB-AF647; C34778, Invitrogen) at the quadrigeminal cistern ...
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bioRxiv - Neuroscience 2020Quote: ... and axial or intercostal muscles were injected with a solution of Cholera Toxin B Subunit-conjugated Alexa555 (Molecular Probes, Life Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... Some mice received a mixture of muscimol and Cholera Toxin Subunit B (Recombinant) Alexa Fluor (647 conjugate, Thermo Scientific) to localize site of infusion ...
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bioRxiv - Cell Biology 2020Quote: ... GM1+ lipid rafts were stained with the cholera toxin-subunit B-AlexaFluor 647 conjugate (1:5000; Molecular Probes, C34778) (58) ...
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bioRxiv - Neuroscience 2022Quote: Neural specific retrograde tracing was conducted with chloral toxin B (CTB-Alexa Fluor 488,10 mg/mL, Molecular Probes®), modulated pseudorabies virus vector (PRV) ...
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bioRxiv - Neuroscience 2020Quote: ... Mice were placed in a stereotaxic apparatus to receive bilateral infusions of the virus or 0.5% (w/v) cholera toxin subunit B (CTb) Alexa Fluor 555 Conjugate (Invitrogen) into the dorsal striatum (+0.1mm antero-posterior ...
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bioRxiv - Neuroscience 2021Quote: ... RGC axons were anterogradely labeled by CTB (Cholera Toxin Subunit B) conjugated with Alexa Fluor™ 555 (Invitrogen, C34776) through intravitreal injection 48 hr before sacrifice ...
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bioRxiv - Neuroscience 2019Quote: ... 2 μl of fluorescence Alexa-594 conjugated cholera toxin β subunit (CTB) (2 μg/μl, Invitrogen; Carlsbad, CA, USA) was micro-injected into the vitreous body using a Picospritzer III (Parker Inc. ...
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bioRxiv - Developmental Biology 2020Quote: ... An approximate total of 500-750 nL of Cholera toxin subunit B (CTB) AlexaFluor 488 or 647 Conjugate (Invitrogen) (Nanoject II ...
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bioRxiv - Neuroscience 2019Quote: ... A second retrograde tracer (Cholera toxin subunit B, Alexa Fluor 647 Conjugate, Invitrogen, 50–60 nl, 1.0 mg/mL) was injected elsewhere to label cells projecting to a different cortical or subcortical area ...
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bioRxiv - Neuroscience 2021Quote: ... mice were mounted in a custom spine stabilizer and viral particles or Cholera Toxin Subunit B conjugated to Alexa Fluor 647 (CTB-647) in sterile 0.9% NaCl (C22841-Thermofisher, Waltham ...
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bioRxiv - Neuroscience 2022Quote: ... Eyes were intravitreally injected using a glass micropipette with 1-5 μl of 0.2% cholera toxin subunit B (CTB) conjugated to Alexa Fluor 488 or 555 (Invitrogen) diluted in 1% DMSO ...
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bioRxiv - Neuroscience 2022Quote: ... mice received a unilateral injection of cholera toxin beta subunit coupled to Alexa Fluor 594 (CTb-594, Invitrogen C34777). Mice were anesthetized with isoflurane and treated with proparacaine ophthalmic drops (1%) ...
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bioRxiv - Neuroscience 2023Quote: ... Retrograde tracers were cholera toxin subunit B (CTB-488; Alexa 488; Molecular probe, Invitrogen, 0.5% in HEPES buffered saline), WGA-Alexa488 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... 1.5 μL of cholera toxin subunit B (CTB) conjugated with Alexa Fluor 594 (2 mg/ml, Thermo Fisher Scientific) was injected into the right vitreous humors of the same mice (same procedure as viral injection ...
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bioRxiv - Microbiology 2023Quote: ... or anti-toxin A HRP-conjugated antibody (LSBio, WA, USA) signal was detected with TMB substrate (ThermoFisher, Waltham, MA) at 450nm using a ELISA plate reader (Berthold ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... a solution containing cholera toxin subunit B (CTb; 1.0 mg/mL) conjugated to Alexa 488 or Alexa 555 (Invitrogen) as a bidirectional tracer was injected into the aDLS and pVLS (0.3 μL/site) ...
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bioRxiv - Genetics 2023Quote: ... Each mouse received a series of injections of Alexa Fluor 488- conjugated cholera toxin B (CTB) (Invitrogen; C-34775). These injections were into the subserosa of the distal colon ...
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bioRxiv - Neuroscience 2019Quote: The retrograde tracers were cholera toxin b subunit (CTb) conjugated to Alexa Fluor 488 (CTb-488) (Cat#C34775 ThermoFisher Scientific) or Alexa Fluor 555 (CTb-555 ...
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bioRxiv - Neuroscience 2021Quote: ... pairwise injections of 80 nL of 0.5% (wt/vol) fluorescently labeled cholera toxin B subunit (CTB-488, ThermoFisher C22841, CTB-647, ThermoFisher C34788). In C57BL6/J mice ...
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bioRxiv - Biophysics 2020Quote: ... The lipid ordered phase was visualized through GM1 crosslinking by incubating the PMS in 10 µg/ml of Alexa-488 labeled cholera-toxin subunit B (Invitrogen) for 2 h at 37°C and IFITM3-iSNAP was stained with 3 µM SNAP-cell 647-SIR (New England Biolabs ...
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bioRxiv - Neuroscience 2020Quote: ... Cholera Toxin subunit-B (Luppi et al., 1995) with a Alexa Fluor-647 (AF-647) conjugate (ThermoFisher, catalog number C34778) was prepared by dissolving 100μg in 20μL PBS ...
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bioRxiv - Neuroscience 2020Quote: The retrograde tracer cholera toxin subunit B (CTB) conjugated with Alexa Fluor™ 555 (CTB-555) was purchased from Invitrogen, Thermo Fisher Scientific (Waltham ...
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bioRxiv - Microbiology 2020Quote: ... Mouse monoclonal antibodies against binary toxin subunits CDTa and CDTb and anti-Clostridia GDH monoclonal mouse antibody (Clone: J44D, Invitrogen) were obtained from Merck ...
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bioRxiv - Biophysics 2020Quote: ... DiIC18(1,1’-Dioctadecyl-3,3,3’,3’-Tetramethylindotri-carbocyanine Iodide) and the Alexa 488-labeled fragment B of cholera toxin were purchased from Thermo Fisher scientific (Waltham ...
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bioRxiv - Neuroscience 2022Quote: Projection neurons were retrograde labeled from their axon terminals by pressure injection of Alexa-conjugated Cholera Toxin B (Ctb, Invitrogen) or Fast Blue (Polysciences ...
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bioRxiv - Neuroscience 2022Quote: ... SCPN (CBN + CSN) or CSN only were retrogradely labeled by bilateral injection of Cholera Toxin B subunit (CTB; Thermo Scientific) into either cerebral peduncles (at 3 injection sites ...
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bioRxiv - Microbiology 2020Quote: ... GM1 coated microtiter plates were incubated with a 1:20 dilution of culture supernatant and detected using primary anti-cholera toxin and secondary HRP-conjugated goat anti-rabbit IgG (Invitrogen). 1-Step Ultra TMB-ELISA (Thermo Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... nwS1 was stereotaxically injected with 80-100 nl cholera toxin subunit B (CTB) conjugated to Alexa Fluor 488 (CTB-488, Invitrogen), and wS1 with CTB-Alexa Fluor 555 (CTB- 555 ...
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Cbln1 directs axon targeting by corticospinal neurons specifically toward thoraco-lumbar spinal cordbioRxiv - Neuroscience 2022Quote: CSN that project to lumbar spinal segments were retrogradely labeled at P5 with an Alexa Fluor 555-conjugated cholera toxin subunit B (CTB-555) recombinant retrograde tracer (Invitrogen). For these injections ...
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bioRxiv - Neuroscience 2023Quote: P12 rats were deeply anesthetized with isoflurane and the sclera and cornea were pierced with a syringe containing a 1% solution of cholera toxin B subunit (Invitrogen) conjugated to either Alexa Fluor 488 (green ...
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bioRxiv - Neuroscience 2023Quote: ... Drd1afl/fl mice received bilateral injections of Alexa Fluor 594-conjugated Cholera Toxin Subunit B in PBS (CTB-594, 1.0 mg mL−1, no. C-34776, Thermo Fisher) in either LH or VTA brain regions.
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bioRxiv - Pathology 2023Quote: Vip3Aa toxin was biotinylated with a 1:30 (protein:biotin) molar ratio of EZ-Link Sulfo- NHS-LC-Biotin (Life Technologies) for 30 minutes on ice ...
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bioRxiv - Bioengineering 2023Quote: ... the bacterial toxin ccdB was cloned with the enzymes KpnI and NheI using a dedicated commercial ccdB-resistant strain (ThermoFisher); the generated plasmid LentiCRISPRV1-gRNA-TetR-intron-ccdB was used to obtain the KpnI/NheI digested backbone for lentiviral library ...