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Citations for Thermo Fisher :
351 - 400 of 10000+ citations for Diazinon Unlabeled 1000 Ug Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Unlabeled HEK 293T and HeLa cells were cultured in Dulbecco’s modified Eagle’s medium (Thermo Fisher Scientific) supplemented with 10 % FBS (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... For the unlabeled primary we incubated with Alexa 546 donkey anti-rabbit IgG (1:500; Invitrogen) diluted in block for 2 days at 37°C on a shaker followed by washing as above ...
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bioRxiv - Molecular Biology 2021Quote: ... 15 ug GlycoBlue co-precipitant (Thermo-Fisher Scientific) and 750 uL 100% EtOH ...
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bioRxiv - Bioengineering 2020Quote: ... RNA (0.5 ug) isolated using TRIzol (Life Technologies) was reverse transcribed using iScript cDNA Synthesis Kit (Bio-Rad) ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 ug Trypsin-LysC (Thermo Fisher Scientific, PRV5073) mix was added to this mixture to allow on-bead digestion of the bound proteins for simultaneous reduction and alkylation of cysteines residues at RT in end-to-end rotor ...
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bioRxiv - Genomics 2023Quote: ... 4 µL RNase A (ThermoFisher, 10 ug/µL) and 12 µL 10 ξ reaction buffer with MgCl2 (ThermoFisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 ug of Turbo DNase (Thermo Fisher Scientific) treated total RNA was reverse transcribed using Maxima reverse transcriptase (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... 500 ug of TMTpro-16plex label (Thermo Fisher) in 100% ACN ...
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bioRxiv - Molecular Biology 2019Quote: ... U2OS cells were resuspended in complete media and 1×105 cells were mixed with pAAVS1-PDi-CRISPRn knockin vector (0.25 ug) and each AAVS1 TALEN pair (0.1 ug) and nucleofected using the Neon® Transfection System (program 17; Life Technologies). Polyclonal population was selected with 2 μg/ml of puromycin (Bioshop cat# PUR555) ...
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bioRxiv - Molecular Biology 2022Quote: ... 3-4 mg total protein and 2 ug of KIF1C antibody or 10 ug mCherry antibody (Invitrogen mCherry Monoclonal Antibody (16D7)) were used ...
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bioRxiv - Microbiology 2021Quote: ... media was removed and replaced with fresh antibiotic-free 10% FBS DMEM containing 20 ug/mL hygromycin (Thermo Fisher Scientific). The concentration of hygromycin was steadily increased until a final concentration of 100 μg/mL was reached ...
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bioRxiv - Genomics 2020Quote: ... Cells were subsequently serum starved on MEF media without FBS and exposed to 0.02 ug/ml Colcemid (Thermo Fisher Scientific/GIBCO) for 12 hours to synchronize and arrest cells in metaphase ...
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bioRxiv - Immunology 2022Quote: Fab generation was initiated with plasmids encoding the truncated heavy chain and light chain of LY-CoV1404 (1 ug/mL each) that were co-transformed into expi293 cells (expifectamine, Gibco) for protein expression (5 days) ...
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bioRxiv - Cancer Biology 2019Quote: ... in tumor digestion medium (RPMI, 10 mM of HEPES, 1x Penicillin-Streptomycin and 20 ug/mL of Gentamicin; Gibco/Invitrogen) in a humidified incubator at 37°C with 5% CO2 and with a gentle rotation for 2-3h to obtain a single cell suspension ...
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bioRxiv - Microbiology 2021Quote: ... and were incubated for 16-20 hrs at 37°C: These plates were respectively screened for presumptive ciprofloxacin-resistant (MacConkey agar infused with 1 ug/mL ciprofloxacin, ThermoFisher) and ceftriaxone-resistant (Brilliance ESBL ...
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bioRxiv - Cell Biology 2022Quote: ... PANC-1 shTAK1 stables were selected using puromycin (10 ug/mL) after small hairpin RNA vector transfection using Lipofectamine 2000 (Invitrogen). Cells were grown in 5% CO2 and at 37°C.
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bioRxiv - Neuroscience 2020Quote: ... Cells were then incubated in primary antibody (rabbit-anti-p65/RELA; 2 ug/mL; ThermoFisher, Waltham, MA, #10745-1-AP) diluted in blocking solution for 1h ...
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bioRxiv - Systems Biology 2021Quote: ... Bafilomycin A1 was added along with a final concentration of 0.2 ug/mL Hoechst 33342 trihydrochloride solution (Hoechst 33342 [Invitrogen, H3570]) to ensure proper mixing ...
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bioRxiv - Immunology 2020Quote: ... DNA was stained for 5 minutes in PBS with 1 ug/ml of DAPI and coverslips mounted with Pro-Long Gold (Invitrogen). Imaging was performed using a Leica SPE2 confocal microscope using LAS X version 3.3.0 software (Leica Microsystems ...
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bioRxiv - Evolutionary Biology 2020Quote: ... we stained the formaldehyde preserves with DAPI (4’,6-diamidino-2-phenylindole, final concentration 5 ug/mL, Thermo Scientific, USA), and counted the microbial cells using hemocytometers (DHC-N01 ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli cells via chemical transformation and plated onto the selective LB agar plates containing 100 ug/ml Ampicillin (Fisher Scientific). The list of E.coli cells can be found in Supplementary Tables S1 ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were transduced with Tet-pLKO-Puro (with shRNAs targeting Cx31 or GFP control) followed by puromycin (2 ug/mL; Gibco) for seven days ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The next day cells were transfected with a mixture of 4 ug plasmid DNA and a 1:3 ratio of PEI (1mg/mL) in Opti-Mem (Gibco). Forty-eight hours later ...
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bioRxiv - Molecular Biology 2023Quote: ... Clones 3 and 5 were transduced with Lenti-rtTA-P2A-Blast viruses and selected in 10 ug/mL Blasticidin (Invitrogen) for 7 days ...
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bioRxiv - Neuroscience 2023Quote: ... was completely exchanged every 48 to 72 hours until DIC 37 with every other media change containing 1 ug/mL laminin (Thermofisher). For astrocyte differentiation ...
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bioRxiv - Neuroscience 2023Quote: ... was used to transfect 0.375 ug of plasmid DNA per mL of Opti-MEM + GlutaMAX (+HEPES + 2.4g/L Sodium Bicarbonate; GIBCO, Life Tech). After 7-16 hours ...
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bioRxiv - Immunology 2023Quote: ... the slides were washed prior to addition of the secondary antibody (goat anti-mouse IgG-Alexafluor488, 5 ug/ml, Invitrogen) and incubated for 2 hours at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... All samples were then incubated with DAPI (4’,6’-diamidino-2-phenylindole) at 1 ug/ml in PBS to visualize DNA and mounted in Prolong Gold mounting media (Invitrogen). Images were acquired on the Zeiss LSM 980 microscope.
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bioRxiv - Cancer Biology 2023Quote: ... The organoids were plated in Matrigel and selection for transduced organoids was performed 3 days after infection by supplementing the organoid culture media with 20 ug/mL blasticidin (Cat# ant-bl-05, Invitrogen).
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bioRxiv - Molecular Biology 2023Quote: ... that was manually coated with 50 ug/mL rat tail collagen I (Ref. no. A10483-01, Gibco, Grand Island, NY) prior to cell plating ...
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bioRxiv - Cancer Biology 2024Quote: ... Transfected cells were selected by visible expression of red fluorescent protein (RFP) and puromycin resistance (0.5 ug/ml) (ThermoFisher Scientific) provided by the HDR plasmid ...
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bioRxiv - Immunology 2024Quote: ... 25,000 rested T cells were co-cultured with DC pulsed with 10 ug/ml cognate peptides or treated with PHA as a positive control (1:100, ThermoFisher). After 5 days ...
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bioRxiv - Immunology 2019Quote: ... 1000 IU/mL IL-4 (Carrier Free, Life Technologies), 100 IU/mL IL-10 (Carrier Free ...
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bioRxiv - Immunology 2023Quote: ... and IL-4 (1000 IU/mL, Invitrogen, MA, USA) for 24 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... and IL-4 (1000 IU/mL, Invitrogen, MA, USA) for 24 hours ...
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bioRxiv - Neuroscience 2023Quote: ... 1000 U/ml penicillin/streptomycin (Thermo Fisher: 15140-122), and 4 ng/ml FGF2 (Thermo Fisher ...
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bioRxiv - Molecular Biology 2020Quote: ... Nascent RNA was separated from unlabeled RNA using MyOne C1 Streptavidin Dynabeads (Thermo Fisher Scientific, 65-001), biotin was removed from nascent RNA using 100 mM dithiothreitol (DTT) ...
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bioRxiv - Microbiology 2020Quote: ... Nascent RNA was separated from unlabeled RNA using MyOne C1 Streptavidin Dynabeads (Thermo Fisher Scientific; 65-001), biotin was removed from nascent RNA using 100 mM dithiothreitol (DTT) ...
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bioRxiv - Cancer Biology 2020Quote: ... the unlabeled medium was replaced with [U-13C6]-glucose medium which composed of low glucose DMEM (Gibco, 11054-020 ...
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bioRxiv - Microbiology 2020Quote: ... 1 ug RNA was used for cDNA synthesis (Invitrogen) prior to PCR analysis following manufacturer’s guidelines (Agilent) ...
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bioRxiv - Biochemistry 2021Quote: ... plasmid pCEN15-UG was digested with NotI (ThermoFisher Scientific) to liberate the integrating fragment containing the CEN locus of Chr XV interrupted by the K ...
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bioRxiv - Molecular Biology 2020Quote: ... at 20U/ug DNA in 1X ligation buffer (Invitrogen). For NlaIII or MulCI digested samples ...
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bioRxiv - Developmental Biology 2023Quote: ... and 2 ug c-FOS antibodies (Invitrogen, MA5-15055) were used in each ChIP reaction ...
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bioRxiv - Immunology 2023Quote: ... and 2 ug NeutrAvidin (Thermo Fisher Scientific, Cat # 31000) in 100 ul PBS per well and incubated overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... 1 ug RNA was used for cDNA synthesis (Invitrogen) prior to PCR analysis following manufacturer’s guidelines (Agilent) ...
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bioRxiv - Cell Biology 2024Quote: ... 1-2 ug of total RNA was DNaseI (Ambion) digested followed by first strand syntheses using random hexamers and Superscript II reverse transcriptase (Invitrogen) ...
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bioRxiv - Genomics 2024Quote: ... 1 ug of total RNA extracted with Trizol (Invitrogen) was submitted to DNAse treatment with Turbo DNAse free kit (Ambion ...
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bioRxiv - Cell Biology 2020Quote: Wild-type and Chmp5Ctsk skeletal progenitors cultured in MatTek glass bottom dishes were incubated on ice for 10 min before adding fresh medium with 2 ug/ml pHrodo™ red EGF conjugate (ThermoFisher). Cells were incubated at 37 °C for 30 min and subsequently washed and changed to fresh media ...
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bioRxiv - Genomics 2020Quote: ... Cells were subsequently serum starved on MEF media without FBS and exposed to 0.02 ug/ml Colcemid (Thermo Fisher Scientific/GIBCO) for 12 hours to synchronize and arrest cells in metaphase ...
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bioRxiv - Cancer Biology 2019Quote: ... 75 µg/ml hyaluronidase and 250 U/mL DNAse I) in tumor digestion medium (RPMI, 10 mM of HEPES, 1x Penicillin-Streptomycin and 20 ug/mL of Gentamicin; Gibco/Invitrogen) in a humidified incubator at 37°C with 5% CO2 and with a gentle rotation for 2-3h to obtain a single cell suspension ...