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Citations for Thermo Fisher :
201 - 250 of 10000+ citations for Dengue Virus serotype 1 lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Virus stocks were diluted into supplemented media (DMEM from Gibco, Cat ...
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bioRxiv - Immunology 2022Quote: ... Virus stocks were diluted into supplemented media (DMEM from Gibco, Cat ...
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bioRxiv - Molecular Biology 2023Quote: ... and virus amplification in Spodopetera frugiperda 9 (Sf9) cells (ThermoFisher) was performed using standard procedures and as described in Zeqiraj et al ...
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bioRxiv - Neuroscience 2023Quote: ... and Moloney murine leukemia virus (M-MLV) Reverse Transcriptase (Invitrogen). One microgram of total RNA was retrotranscribed and the cDNA was diluted 1:10 to the quantitative PCR (qPCR) ...
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bioRxiv - Immunology 2024Quote: ... Virus inoculum was prepared in DMEM (Thermo Fisher Scientific, 119905040) supplemented with 2% FBS and allowed for 1 hour at 37°C for virus adsorption onto cells ...
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bioRxiv - Immunology 2024Quote: ... Antibodies used: anti-Influenza A virus NS1 (PA5-32243, ThermoFisher), anti-acetyl-α-tubulin (Ly640 ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysate equivalent to 1-2 × 105 cells was resolved by SDS-PAGE (Life Technologies) and transferred to nitrocellulose membranes (Amersham) ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mg chromatin lysates were incubated with 50 µl Dynabeads Protein A (Invitrogen 10001D) coated with 10 µg GINS antibodies for 2 h at 4°C with rotation ...
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bioRxiv - Biochemistry 2022Quote: ... The lysate was ultracentrifuged at 31,000 RCF for 1 hour (Thermo Fisher Scientific centrifuge), to remove cell debris and suspended particles ...
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bioRxiv - Cell Biology 2023Quote: ... Cleared lysates were incubated for 1 hour at 4DC with Dynabeads Streptavidin T1 (Invitrogen) (8µl beads per sample) ...
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bioRxiv - Bioengineering 2023Quote: ... Clarified lysate was loaded onto 1 mL of Ni-NTA resin (Thermo Fisher Scientific) pre-equilibrated in wash buffer ...
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bioRxiv - Cell Biology 2022Quote: ... Cell lysates were prepared using 1 ml RIPA lysis and extraction buffer (ThermoFisher Scientific), supplemented with cOmplete EDTA-free protease inhibitor cocktail (Roche ...
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bioRxiv - Immunology 2023Quote: Cell lysates and supernatants were boiled for 10 min in 1× NuPAGE LDS (Thermofisher) or in-house (2 % w/v SDS ...
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bioRxiv - Neuroscience 2024Quote: ... 0.5 to 1 mg of cell lysate was digested with PK (ThermoFisher Scientific #EO0491) at a concentration of 50 µg/mL and a PK:protein ratio of 1:50 ...
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bioRxiv - Molecular Biology 2024Quote: ... 1/10 diluted lysates were quantified using the Pierce BCA protein assay (Thermo Scientific). 30 μg of protein lysates were mixed with equal parts 2x Loading buffer (21% w/v glycerol ...
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bioRxiv - Microbiology 2023Quote: We initially obtained baculovirus carrying the genetic information of full-length rhesus rotavirus VP7 (G3 serotype) from bacmid transfected Sf9 cells (Thermo Fisher Scientific). Sf9 cells were then inoculated with baculovirus and passaged in the same cells three times ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Copies of SARS-CoV-2 E gene were measured by qRT-PCR TaqMan Fast Virus 1-step assay (Thermo Fisher). SARS-CoV-2 specific primers and probes from the 2019-nCoV RUO Assay kit (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2021Quote: ... 1μL of each extracted RNA was used to performed one step RT-qPCR using TaqMan Fast Virus 1-Step Master Mix (Invitrogen). The relative quantities of envelope (E ...
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bioRxiv - Microbiology 2020Quote: ... qRT-PCR was performed using 100ng total RNA compared to an RNA standard curve using TaqMan Fast Virus 1-Step Master Mix (ThermoFisher) on a Quant Studio 3 (Applied Biosystems).
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bioRxiv - Microbiology 2020Quote: ... Viral RNA was quantified by RT-qPCR using the TaqMan(tm) Fast Virus 1-Step Master Mix (Thermo Fisher Scientific). Primers and probes were based on (21 ...
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bioRxiv - Cell Biology 2021Quote: ... Viral RNA was quantified by RT-qPCR using the TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher Scientific). Primers and probes were used as described previously (45) ...
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bioRxiv - Immunology 2022Quote: ... The extracted RNA was then added to TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher Scientific, Waltham, MA) containing primers and a fluorescently labeled hydrolysis probe specific for mRNA from the nucleocapsid gene of SARS-CoV-2 (forward primer 5’-CGATCTCTTGTAGATCTGTTCTC-3’ ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 RNA was detected with the CDC assay kit (IDT) and the TaqMan Fast Virus 1-Step Master Mix (ThermoFisher) on the QuantStudio3 System (Invitrogen).
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bioRxiv - Microbiology 2022Quote: ... The nCoV_IP2 primer-probe set was used in combination with TaqMan fast virus 1-step master mix (Thermo Fisher Scientific) to detect viral RNA ...
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bioRxiv - Microbiology 2022Quote: ... 1μL of each extracted RNA was used to performed one step RT-qPCR using TaqMan Fast Virus 1-Step Master Mix (Invitrogen). The relative quantities of envelope (E ...
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR was performed as previously described(42) using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher, Cat#4444436) and a OneStepPlus Real-Time PCR System (96-well format ...
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bioRxiv - Microbiology 2021Quote: ... qRT-PCR was performed as previously described [39] using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher, Cat#4444436) and a OneStepPlus Real-Time PCR System (96-well format ...
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bioRxiv - Immunology 2021Quote: ... dilutions of IgG were co-incubated with the virus (1000-2000 TCID50) for 1 h at 37°C prior to addition of VeroE6 cells in DMEM (Gibco) containing 2% FBS ...
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bioRxiv - Immunology 2020Quote: ... 1μL of each extracted RNA was used to performed one step RT-qPCR using TaqMan Fast Virus 1-Step Master Mix (Invitrogen). The relative quantities of nucleocapsid (N ...
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bioRxiv - Microbiology 2022Quote: Blood virus mixtures were titrated by ten-fold serial dilution in virus media (RPMI 1640 cell culture media supplemented with 5% fetal bovine serum [FBS] and 1% Penicillin-Streptomycin [Gibco]) in a 96-well plate before transferring dilutions to equivalent wells of a 96 well plate containing near confluent C6/36 cell monolayers ...
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bioRxiv - Microbiology 2022Quote: ... The RT-qPCR was performed according to the manufacturer’s protocol using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher). Fluorescent signals were acquired using QuantStudio 6 Real-time PCR system (Thermo Fisher).
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bioRxiv - Plant Biology 2023Quote: ... RT-PCR was performed with 1 µg of RNA using 100 units of Moloney murine leukemia virus reverse transcriptase (Invitrogen) according to instruction of the manufacturer ...
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bioRxiv - Genetics 2021Quote: ... PCR reactions contained 1 μl of 1:10 diluted lysate and 5 μl premixed AmpliTaq-Gold (0.3U, Life Technologies) with 0.26 μM of target-specific- and an additional fluorescent forward primer ...
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bioRxiv - Neuroscience 2022Quote: ... 1 mg lysate per sample was incubated with Pierce streptavidin magnetic beads (Thermo Scientific, 88817) for 30 min at 4°C in rotation ...
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bioRxiv - Molecular Biology 2021Quote: 4 μL of the crude lysate (representing 0.4 %) in 1 x NuPAGE loading dye (Invitrogen) and 10 μL of boiled beads (representing 4 % ...
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bioRxiv - Neuroscience 2020Quote: ... the lysates were incubated for 1 hour with 2ug anti-GFP antibody (Thermo Fisher Scientific) and then incubated for 30-60 minutes with Protein A Dynabeads (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... Ten microliters of cleared lysate were mixed with 1 ml TRIzol (Thermo Fisher, cat# 15596026) for the RNA input control ...
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bioRxiv - Microbiology 2022Quote: ... Then the cell lysates were treated with 1 × Halt protease inhibitor cocktail (Thermo Fisher Scientific) and 100 U/mL Benzonase endonuclease (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 mg lysate was pre-cleared with Dynabeads™ protein G (cat# 10004D, Thermo Fisher) for 1 h with rotation at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... the remaining lysate precleared for 1 hour with Protein-A Dynabeads (Thermo Scientific, no. 10001D). FLAG-M2 antibody Protein-A Dynabead conjugate was incubated with the precleared lysate overnight ...
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bioRxiv - Cell Biology 2023Quote: ... 500 μg precleared cell protein lysate was treated with 1 U RNase I (Ambion AM2294) for 5 min at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... virus stocks were prepared in serum-free VP-SF media (Invitrogen). Viruses were harvested before CPE development ...
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bioRxiv - Cell Biology 2021Quote: ... The virus packaging was performed in HEK293FT cells (Thermo Fisher Scientific) based on a calcium precipitation method using pUMVC and pCMV-VSV-G vectors (37 ...
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bioRxiv - Immunology 2021Quote: ... Virus particles in 2% FBS/DMEM/1mM Pen/Strep (Thermofisher, 15140122) were incubated with cells for 2 hours at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... cells and purified virus particles were lysed with RIPA (ThermoFisher Scientific) buffer supplemented with complete protease inhibitor cocktail (Roche ...
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bioRxiv - Microbiology 2020Quote: ... Virus stocks were treated with 40 U/mL DNase I (Invitrogen) for 1 hr at 37°C before they were used to infect cells for 2 hr at an MOI of 0.5-2 ...
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bioRxiv - Physiology 2022Quote: ... and 200 U of Moloney murine leukemia virus reverse transcriptase (Invitrogen) in a final reaction volume of 20 μL (Evans-Molina et al. ...
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bioRxiv - Genetics 2020Quote: ... Cells were then transduced using CytoTune-iPS 2.0 Sendai virus (Invitrogen) as outlined in the supplier’s protocol at an MOI of 5:5:3 (KOS [Klf4 ...
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bioRxiv - Microbiology 2021Quote: Virus replication assays were performed in RPMI 1640 (Gibco 11875-093) supplemented with 10% FBS ...
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bioRxiv - Biochemistry 2023Quote: ... virus inoculum was exchanged with serum-free MEM (Gibco, Life Technologies) containing 0.75% carboxymethylcellulose (Sigma-Aldrich ...