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Citations for Thermo Fisher :
601 - 650 of 10000+ citations for Dengue Virus serotype 1 lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... the concentration of a 1:10 dilution of the lysate (1:50 for the egg with yolk) was measured with a reducing agent compatible BCA assay (ThermoFisher 23252).
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bioRxiv - Molecular Biology 2020Quote: ... the rest of the clarified lysate was then incubated with 25 uL Protein G magnetic beads (Pierce) coupled with 1 μL mouse anti-V5 (Life Technologies) antibody for 2 hours at 4°C with rotation ...
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bioRxiv - Cell Biology 2022Quote: ... 30 to 50 μl of cell lysate was digested using a 5:1 mixture of nitric acid (OPTIMA grade, 70%, Fisher Scientific) and ultrapure hydrogen peroxide (ULTREX II ...
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bioRxiv - Cell Biology 2022Quote: ... were added to 1-2 mg of lysates for overnight incubation at 4 °C before the incubation with protein G Dynabeads (Life Technologies) for additional 2 hrs ...
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bioRxiv - Biochemistry 2022Quote: 250 μg of protein from HEK-293T cell lysates were incubated overnight with 2 μg of a 6x-His tag antibody (MA 1-135, Thermo Fisher) in the presence of a low–salt RIPA buffer (20mM Tris-HCl ...
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bioRxiv - Molecular Biology 2020Quote: Frozen cell powder was resuspended in lysis buffer (1:3 w/v) and the lysate was cleared by centrifugation in an SS-34 rotor (Thermo Scientific) at 26891.8 × g for 15 min.
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bioRxiv - Neuroscience 2022Quote: ... and 1 μL of the crude lysate was directly utilized as input for PCR reactions (DreamTaq Green PCR Master Mix (Thermo Fisher) supplemented with 1M betaine and 3% DMSO (Sigma Aldrich)) ...
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bioRxiv - Molecular Biology 2022Quote: Total 40μg of protein from cell lysates were loaded in 8%SDS-PAGE gel and immunoblotted with RAD51 antibody (1:1000; PA5-27195; Thermofisher Scientific) diluted in 1%BSA+TBS+ 0.1%Tween 20 ...
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bioRxiv - Neuroscience 2022Quote: ... Zebrafish genotypes were determined by incubating fin-clip lysate with indel-primers (Table 1) and DreamTaq PCR Master Mix (Thermo Scientific) at 95°C (3 min ...
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bioRxiv - Synthetic Biology 2022Quote: ... 4.75 μL of nuclease-free water and 400 nM of each primer or by adding 1 µl of yeast lysate to 5 µl of DreamTaq Green PCR Master Mix (2X) (ThermoFisher Scientific) plus 4 µL of nuclease-free water with 200 nM of each primer ...
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bioRxiv - Cell Biology 2023Quote: ... 1 µL of DNA lysate was added to a 20 µL amplification reaction (Phusion Plus Green PCR Master Mix, ThermoFisher, F632L). 400-600 bp fragments flanking the edited regions were amplified ...
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bioRxiv - Cell Biology 2023Quote: ... These constructs were then used for in vitro translation using a HeLa cell lysate-based Kit (1-Step Human Coupled IVT Kit—DNA, 88881, Life Technologies). The in vitro-translated proteins were then purified using His Pur cobalt spin columns (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... was equilibrated with lysis buffer and then incubated with the cell lysate at 4 °C for 1 h on an end-to-end rotator (Cat# 88881001, ThermoFisher scientific). The slurry was transferred to a 15 ml falcon tube ...
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bioRxiv - Synthetic Biology 2023Quote: ... The lysates were then treated with 2.5 U mL−1 Pierce™ universal nuclease for cell lysis (Thermo Fisher Scientific Co.) at room temperature with gentle shaking for 30 min ...
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bioRxiv - Bioengineering 2024Quote: The cellular lysates were prepared using Protein Extraction Solution (ELPISBIO, #EBA-1149) supplemented with 1% Halt Protease and Phosphatase Inhibitor (ThermoFisher, #78442), followed by a 30-min incubation on ice ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes containing whole-cell lysates were also incubated with an HRP-conjugated rabbit anti-β-actin (Invitrogen MAB-32540, 1:1000). The Pierce ECL (Amersham ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysates were quantified by BCA assay (Cat # PI23224, Thermo Scientific). Samples were prepared with NuPage 10x Reducing Agent (Cat# NP0009 ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysates were quantified with Quant-iT Ribogreen assay (Life Technologies) and 60 ug total RNA per sample was incubated with 3 uL RNase I (Epicentre #N6901K ...
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bioRxiv - Cell Biology 2020Quote: ... Lysate concentration was measured using Qubit Protein Assay Kit (Invitrogen) and Qubit 4 Fluorometer ...
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bioRxiv - Cell Biology 2020Quote: ... proteins in the lysate were digested by Proteinase K (Ambion), RNA was cleaned up using SPRI beads (GE ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein lysates were loaded with LDS Sample Buffer (Invitrogen, NP0007) and Reducing Agent (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... Lysates were then precleared with Protein G Dynabeads (ThermoFisher; 10004D) at 4°C for an hour ...
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bioRxiv - Molecular Biology 2021Quote: Lysates were precleared on Protein G-magnetic Beads (Thermo Fisher) for 1 hour at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysates were precleared with Dynabeads Protein-G (Novex, Life Technologies) for 1hr ...
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bioRxiv - Cell Biology 2022Quote: Lysates were run on 4-12% polyacrylamide gels (NuPage, Invitrogen) for 50 minutes at 180V and transferred to polyvinylidene difluoride (Immobilon-FL ...
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bioRxiv - Molecular Biology 2019Quote: ... Lysates were pre-cleared using protein-A Dynabeads (Invitrogen, 10002D), 1 hour at 4°C ...
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bioRxiv - Immunology 2019Quote: ... Cell lysates were pre-cleared using protein A beads (Invitrogen). Pre-cleared lysates were immunoprecipitated using anti-lamin B1 antibody and these immunoprecipitated samples were incubated with protein A-sepharose beads (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... the lysate was incubated with 250ul streptavidin resin (Thermo Fisher) for 1.5hr ...
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bioRxiv - Immunology 2021Quote: ... lysates were incubated with streptavidin magnetic beads (Pierce Thermo Fisher) for 1h at room temperature on a rotator to bind biotinylated proteins ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells lysates were reverse transcribed using Superscript™ III (Invitrogen) and pre-amplified for 20 rounds using a custom-designed set of target-specific primers ...
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bioRxiv - Microbiology 2020Quote: ... RNA from cell lysates were collected using Trizol reagent (Invitrogen). RNA was then extracted from Triazol using the Direct-zol RNA Miniprep Plus kit (Zymo Research #R2072 ...
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bioRxiv - Systems Biology 2021Quote: ... Lysates were analyzed with SDS – polyacrylamide gel electrophoresis gels (Invitrogen). Proteins were transferred onto polyvinylidene difluoride (PVDF ...
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bioRxiv - Microbiology 2020Quote: ... Whole cell lysates were added to His beads (Thermo Scientific HisPur Ni-NTA Magnetic Beads ...
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bioRxiv - Cancer Biology 2021Quote: Total cell lysates were isolated using RIPA buffer (ThermoFisher Scientific) in the presence of a protease inhibitor mixture (Roche ...
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bioRxiv - Biochemistry 2022Quote: ... and the cleared lysate bound to Ni-NTA resin (Invitrogen) overnight at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... Clarified lysates were applied to HisPur cobalt affinity resin (ThermoFisher) and washed with Buffer A prior to elution with Buffer A containing 300 mM imidazole ...
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bioRxiv - Microbiology 2020Quote: ... and the lysates were homogenised using the Homogenizer columns (Invitrogen) as per the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2021Quote: Whole cell lysates were generated using M-PER™ (ThermoFisher) with 1X Halt™ protease and phosphatase inhibitor (ThermoFisher) ...
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bioRxiv - Cell Biology 2020Quote: ... the lysate was treated with RNase A/T1 (Fisher Scientific) for 1 hour at room temperature and immediately immunoprecipitated ...
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bioRxiv - Immunology 2020Quote: ... Protein concentration of lysates was assessed by Bradford Assay (Thermofisher) and 20 μg of protein per sample loaded on polyacrylamide gels ...
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bioRxiv - Immunology 2021Quote: ... Cell lysates were prepared using Denaturing Cell Extraction Buffer (Invitrogen) containing protease & phosphatase inhibitor cocktail (ThermoScientific) ...
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bioRxiv - Synthetic Biology 2019Quote: ... Phagebody lysates were treated with TURBO DNase (Thermo Fisher Scientific) to degrade any soluble plasmid not encapsulated by the phage and then heat inactivated ...
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bioRxiv - Biochemistry 2019Quote: Cell lysates were separated on a SDS-polyacrylamide gel (Invitrogen), and transferred to a nitrocellulose membrane using a wet transfer system (BioRad) ...
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bioRxiv - Immunology 2019Quote: Cell lysates were prepared in RIPA buffer (Thermo Fisher scientific) in the concentration of 80ul lysis buffer per 1 × 106 cells ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein lysates were analysed by SDS-PAGE and immunoblotting (Invitrogen). Bound antibodies were visualised with horseradish peroxidase-conjugated anti-IgG antibodies and enhanced chemiluminescence susbtrates (GE).
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bioRxiv - Immunology 2019Quote: ... lysates were run on 3-8% Tris-acetate gels (ThermoFisher) for 2 hours at 150 V and then transferred in 5% methanol for 3 hours at 20 V at 4°C.
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bioRxiv - Immunology 2019Quote: ... Lysates were immunoprecipitated with control mouse immunoglobulin G (IgG) (Invitrogen) or anti-Flag antibody (Sigma ...
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bioRxiv - Biochemistry 2020Quote: ... Sonicated cell lysate was combined with Ni-NTA resin (Invitrogen) and purified via the his-tag ...
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bioRxiv - Physiology 2020Quote: ... Protein lysates were separated using 4-12% SDS-PAGE (Invitrogen) and transferred to nitrocellulose membranes (Bio-Rad) ...
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bioRxiv - Molecular Biology 2021Quote: ... cell lysates were prepared using the Trizol reagent (Thermo Scientific) and the viral RNA content was determined by RT-qPCR using previously validated sets of primers and probes specific for the detection of the SARS-CoV-2 E gene and the cellular 18S gene ...