Labshake search
Citations for Thermo Fisher :
601 - 650 of 10000+ citations for Dengue Virus Subtype 4 Envelope 45 kDa Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: The lysates were analyzed by 4-16% Native PAGE using precast Bis-Tris Mini Protein Protein Gels (Invitrogen BN1003BOX) following the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2022Quote: ... Lysates were cleared of insoluble material by centrifuging at 20,000 g for 10 min at 4°C and protein concentration was determined with the BCA Protein Assay (ThermoFisher). For immunoblotting 20-40 μg of protein in equal volumes were heated in LDS-sample buffer (Invitrogen ...
-
bioRxiv - Molecular Biology 2021Quote: ... A total of 5 μg protein was run on a NuPage™ 4-12% Bis-Tris Protein Gel (Invitrogen) using MOPS buffer according to manufacturer instructions and subsequently transferred to a nitrocellulose membrane according to the NuPage™ manufacturer's protocol (Invitrogen) ...
-
Mitochondrial dysfunction rapidly modulates the abundance and thermal stability of cellular proteinsbioRxiv - Biochemistry 2022Quote: ... 4 μl of the flow through were subjected to a protein determination (Pierce BCA Protein Assay (Thermo Scientific, #23225). 25 μl of the remaining flow-through were mixed 1:1 with 2x sample buffer (180 mM Tris pH 6.8 ...
-
bioRxiv - Developmental Biology 2022Quote: ... 10 µg protein of each sample was loaded into 4 to 12% Bis-Tris protein gel (Thermo Fisher Scientific) and sent for MS analysis at the University of Texas System Proteomics Network ...
-
bioRxiv - Neuroscience 2023Quote: ... Equal amounts of protein were then loaded onto a NativePAGE Novex 4-16% Bis-Tris Protein Gel (Life Technologies). A wet blotting method was used to transfer the proteins onto a PVDF membrane (Life Technologies ...
-
bioRxiv - Microbiology 2023Quote: ... separating proteins by electrophoresis with Chameleon Pre-Stained Protein Ladder (Li-Cor) on 4–12% Bis-Tris Gels (Invitrogen) at 150 V for 90 min ...
-
bioRxiv - Cell Biology 2023Quote: ... Proteins were separated in an NuPAGE™ Novex™ 4-12% Bis-Tris protein gel (WG1402BOX, Thermo Fisher Scientific) in NuPAGE™ MOPS SDS running buffer (50 mM MOPS ...
-
bioRxiv - Cell Biology 2023Quote: Lysates containing 25 µg of protein were electrophoresed on a NuPage 4%-12% Bis-Tris Protein gel (Thermofisher Scientific). Proteins were transferred to a PVDF membrane (0.2 µm ...
-
bioRxiv - Molecular Biology 2023Quote: ... Equivalent protein quantities for all genotypes were loaded onto a Nupage 4-12% Bis-Tris Protein Gels (ThermoFisher Scientific) for 1 hour ...
-
bioRxiv - Cancer Biology 2023Quote: ... Lysates were clarified by centrifugation at 4°C and the protein concentration was determined by BCA protein assay (Thermofisher). Samples were resolved on a precast 4%–12% gradient gel (Thermo Fisher Scientific ...
-
bioRxiv - Developmental Biology 2023Quote: ... using the Qbit 4 fluorometer (with the Broad range protein assay kit Qubit Protein BR Assay, from ThermoFisher Scientific), and sugar concentration using the refractometer (refractometer RBR32-ATC) ...
-
bioRxiv - Molecular Biology 2023Quote: ... 6000 rpm 4°C and protein concentration was estimated by BCA protein assay kits (Thermo-Fisher Scientific, Cat #23225).
-
bioRxiv - Genomics 2024Quote: ... was coupled for 4 hours to 40µl of 1:1 mix Protein G and Protein A Dynabeads (Thermo Scientific) and used for overnight chromatin immunoprecipitation in IP buffer (1% Triton X-100 ...
-
bioRxiv - Microbiology 2024Quote: ... then cooled and measured for total protein by Qubit 4 using the Protein Assay Quantification Kit (Life Technologies, Q33211), according to manufacturer’s instructions.
-
bioRxiv - Neuroscience 2020Quote: ... the sEV-containing supernatant from the 10K step was concentrated with a 100 kilodalton (kDa) molecular weight cut-off (MWCO) protein concentrator (Thermo Fisher 88524) from 5 ml to 0.5 ml before application onto qEV Original SEC columns (IZON Science SP1-USD ...
-
bioRxiv - Molecular Biology 2022Quote: ... or Cdc3 were diluted in PBS and centrifuged through 0.5 mL 100-kDa molecular-weight cutoff protein concentrators (Thermo Scientific Pierce #PI88503) according to the manufacturer’s instructions ...
-
bioRxiv - Systems Biology 2019Quote: ... Total protein extracts for each sample were quantified by bicinchoninic acid assays and 100 μg proteins were digested on 10-kDa MWCO polyethersulfone filters (Thermo Fisher Scientific). Samples were washed with 8 M urea ...
-
bioRxiv - Biochemistry 2021Quote: ... The peak eluting around 14 mL was concentrated using a 100 kDa cutoff filter column (Amicon Centrifugal Filter Device) and the protein concentration was measured using BCA assay (Pierce, Thermo Fisher Scientific). The aliquoted OST complexes were shock frozen in liquid nitrogen before stored at -80 °C.
-
bioRxiv - Biochemistry 2022Quote: ... Free dye was removed from labeled protein by buffer exchanging labeling reactions three times using a ZebaTM Spin Desalting Column (7 kDa MWCO, Thermo Scientific #89890) into 50 mM Tris-HCl (pH 7.9) ...
-
Evolutionary stasis of an RNA virus indicates arbovirus re-emergence triggered by accidental releasebioRxiv - Evolutionary Biology 2019Quote: ... and virus isolates using Trizol LS® (Invitrogen, USA) and purified using Direct-zol RNA MiniPrep (Zymo Research ...
-
bioRxiv - Cell Biology 2022Quote: ... or Sendai virus system Cytotune 2.0 (Thermo Scientific #A16517). Resulting iPSCs were clonally picked and expanded in mTESR (Stemcell Technologies ...
-
bioRxiv - Microbiology 2019Quote: ... The virus inoculum was prepared in OPTI-MEM (Gibco), and cells were incubated in SMEM at 37°C ...
-
bioRxiv - Microbiology 2021Quote: ... Pelleted virus was resuspended in PBS and TRIzol (Invitrogen) extracted ...
-
bioRxiv - Microbiology 2022Quote: ... and from virus in supernatant using Trizol LS (ThermoFisher). A one-step qRT-PCR kit with SYBR green from Agilent was used ...
-
bioRxiv - Microbiology 2021Quote: ... virus stocks were diluted in OptiPRO SFM (ThermoFisher Scientific) serum-free medium according to the desired MOI ...
-
bioRxiv - Microbiology 2020Quote: ... The virus pellet was washed in HBSS (Thermo Fisher), and centrifuged at 100,000 × g (Beckman SW 32 Ti rotor ...
-
bioRxiv - Immunology 2022Quote: ... and Moloney murine leukemia virus (MMLV) reverse transcriptase (Invitrogen), followed by RT-PCR with Fast SYBR Green PCR Master Mix (Applied Biosystems ...
-
bioRxiv - Microbiology 2020Quote: Virus stocks were prepared by transfecting 293FT cells (Invitrogen) seeded in 10 cm cell culture dishes with the viral constructs described above ...
-
bioRxiv - Microbiology 2023Quote: ... TaqMan Fast Virus 1-step Master Mix (Applied Biosystems) and primer/probes targeting NS3 primers/probe (IDT)11 ...
-
bioRxiv - Microbiology 2024Quote: ... and TaqMan Fast Virus 1-Step Master Mix (ThermoFisher). Reactions were set up according to the manufacturer’s protocols using a 10 µl reaction volume and 25 ng of total RNA template ...
-
bioRxiv - Cancer Biology 2024Quote: ... virus was prepared in high-glucose DMEM (Gibco, #11965092) supplemented with 10% FBS and 1% penicillin–streptomycin ...
-
bioRxiv - Microbiology 2021Quote: ... MHV-A59 was quantified by RT-qPCR by amplifying a fraction of its ORF5 gene for the structural protein M.15,16 The TaqMan™ Fast Virus 1-Step Master Mix Kit (Thermo Fisher Scientific Inc., 4444432) was used for RT-qPCR ...
-
bioRxiv - Immunology 2021Quote: The RBDwt protein was conjugated to Cucumber mosaic virus - tetanus toxoid (CuMVTT) using the linker Succinimidyl 6-(beta-maleimidopropionamido) hexanoate (SMPH) (Thermo Fisher Scientific, Waltham, USA). CuMVTT contains a powerful T cell epitope of the Tetanus toxin (TT ...
-
bioRxiv - Cell Biology 2023Quote: ... 10mM desthiobiotin, 1mM EDTA, 2mM DTT) and concentrated using an Amicon Ultra Centrifugal Filter Unit (50 kDa, 4 ml) (Fisher Scientific, EMD Millipore). Proteins were further purified by gel filtration chromatography (Superdex-200 ...
-
bioRxiv - Cell Biology 2023Quote: ... 5 % glycerol, 50 mM maltose) and were concentrated using an Amicon Ultra Centrifugal Filter Unit (50 kDa, 4 ml) (Fisher Scientific, EMD Millipore). Proteins were further purified by gel filtration chromatography (Superdex-200 ...
-
bioRxiv - Molecular Biology 2020Quote: ... 45 μL SuperScript III reverse transcriptase (200 U/μL, Invitrogen), and 75 μL nuclease-free water (Biological Industries ...
-
bioRxiv - Cancer Biology 2021Quote: ... collagenase type IV (45 units/mL, Gibco, Thermo Fisher Scientific), elastase (0.08 units/mL ...
-
bioRxiv - Cancer Biology 2021Quote: ... collagenase type IV (45 units/mL, Gibco, Thermo Fisher Scientific), elastase (0.08 units/mL ...
-
bioRxiv - Bioengineering 2021Quote: ... blocked for 45 minutes using 5% goat serum (ThermoFisher #16210064) in PBS with 0.05% sodium azide (Sigma #S2002) ...
-
bioRxiv - Neuroscience 2021Quote: ... for 45 mins (or MES buffer (Nupage Thermo Fisher Scientific) for 35 min in case of Iba-1 only ...
-
bioRxiv - Immunology 2021Quote: ... supplemented with gentamicin (45 µg/ml final concentration; Gibco #15710064), HEPES (40 mM ...
-
bioRxiv - Cell Biology 2022Quote: ... blocked for 45 min with 5% goat serum (ThermoFisher #16210064) in DPBS with 0.05% sodium azide (Sigma #S2002) ...
-
bioRxiv - Neuroscience 2020Quote: ... Oregon Green 488 BAPTA-1 hexapotassium salt 45 μM (Invitrogen); Fluorescein Dextran 2000 kDa 12.5 μM (Sigma Aldrich) ...
-
bioRxiv - Immunology 2022Quote: ... anti-CD44-PerCP5.5 (Invitrogen 45-0441-82: Clone: IM7, eBioscience), anti-TCRv β14-FITC (BD Biosciences 553258 ...
-
bioRxiv - Neuroscience 2022Quote: ... Cells were cultured in 45% DMEM-Glutamax (gibco, ThermoFisher SCIENTIFIC), 45% Opti-MEM (gibco ...
-
bioRxiv - Neuroscience 2023Quote: ... while gfCDM alone comprised 45% Iscove’s modified Dulbecco’s medium (Gibco), 45% Hams F12 (Gibco) ...
-
bioRxiv - Cell Biology 2023Quote: ... OxPhos Rodent WB Antibody Cocktail (1:250, #45-8099 Invitrogen) and β-actin (1:1000 ...
-
bioRxiv - Microbiology 2023Quote: ... supplemented with gentamicin (45 µg/ml final concentration; Gibco #15710064), HEPES (40 mM ...
-
bioRxiv - Cancer Biology 2023Quote: ... OxPhos Human WB Antibody Cocktail (Thermo-Fisher Scientific #45-8199) and β-actin (Sigma-Aldrich ...