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Citations for Thermo Fisher :
351 - 400 of 10000+ citations for Dengue Virus Serotype 4 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... 20 μg protein was loaded onto a 4-12% bis-tris gradient protein gel (Invitrogen) under reducing conditions and transferred onto PVDF membrane ...
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bioRxiv - Physiology 2024Quote: ... The denatured proteins (20 µg/well) were separated by 4-12 % protein gel (Thermo Scientific) and transferred onto PVDF membranes (MiliporeSigma ...
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bioRxiv - Physiology 2024Quote: ... Proteins were separated on 4-12% Bis-Tris Protein Gels (NuPage Novex, Thermo Fisher Scientific) and transferred to nitrocellulose membrane (GE Healthcare Life Sciences ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were separated using a 4-12% Bis-Tris Protein SDS-PAGE Gel (Life Technologies). Samples were transferred to a nitrocellulose membrane (Thermo Fisher ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins separated by NuPAGE™ 4-12% Bis-Tris precast protein gels (Thermo Fisher Scientific), and then transferred to nitrocellulose membrane (GE Healthcare) ...
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bioRxiv - Cell Biology 2021Quote: ... diluted cell supernatant was preabsorbed for one hour at 4°C with protein A/G magnetic beads (Invitrogen). The supernatant fraction was incubated overnight at 4°C with an appropriate antibody and protein A/G magnetic beads were blocked with 4% BSA ...
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bioRxiv - Developmental Biology 2021Quote: Whole-cell lysates and immunoprecipitated proteins were subjected to NuPAGE 4%-12% bistris acrylamide gel electrophoresis (Life Technologies). Using standard protocols ...
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bioRxiv - Bioengineering 2022Quote: ... Equal protein amounts of nuclear cell lysates were run on 4-20% gradient Tris-glycine gels (Novex-Invitrogen), transferred to PVDF membranes using an iBlot Gel Transfer Device (ThermoFisher) ...
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bioRxiv - Cell Biology 2022Quote: ... whole protein extracts from fungus or host cells were separated on NuPAGE 4%–12% Bis-Tris Gels (Invitrogen) and transferred to 0.2-µm pore size PVDF membranes (Invitrogen ...
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bioRxiv - Biochemistry 2019Quote: Total cell lysates or cellular fractions (as indicated) were resolved in 4-12% Bis-Tris Protein Gels (Invitrogen) and transferred in for 1.5 h (80 V ...
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bioRxiv - Cancer Biology 2019Quote: ... 8-30μg of whole-cell extract was separated on precast 4 to 12% bis tris protein gel (Invitrogen) and transferred to a nitrocellulose membrane ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein (20 μg) from extracted cell lysate was subjected to SDS-PAGE (4-12% NuPAGE gel, Life Technologies), transferred to nitrocellulose membranes ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell lysis protein samples were separated by gel electrophoresis on 4-20% ready-made Tris-Glycine gels (Invitrogen) and transferred at 110mV for one hour to PVDF membranes (Millipore) ...
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bioRxiv - Microbiology 2022Quote: ... pneumoniae serotype 2 strain D39 [17] was plated on brain heart infusion (BHI, Thermo Scientific, MA, USA) agar plates supplemented with 3% v/v defibrinated horse blood (Thermo Scientific ...
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bioRxiv - Immunology 2020Quote: The H99S strain [21] of Cn serotype A was grown in yeast peptone dextrose (YPD; ThermoFisher Scientific) broth ...
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bioRxiv - Microbiology 2020Quote: ... and transfected for virus reconstitution into BHK-21 cells with Lipofectamine 3000 (ThermoFisher #L3000-075).
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bioRxiv - Immunology 2020Quote: ... RAW264.7 cells were then transduced with virus and selected using hygromycin at 10mg/ml (Invitrogen) to select for cells containing the shRNA plasmid.
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bioRxiv - Cell Biology 2022Quote: Cell lines were generated using Sendai virus CytoTune-iPS 2.0 Sendai reprogramming kit (Thermo Fisher) for transfection of Yamanaka’s factors ...
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bioRxiv - Immunology 2022Quote: ... RNA from virus-infected cells was isolated using PureLink RNA Mini Kit (Thermo Fisher Scientific), following manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... and transfected for virus reconstitution into BHK-21 cells with Lipofectamine 3000 (ThermoFisher #L3000-075). HSV-1 expressing the FLAG-tagged N-terminal extension of UL54 virus were reconstituted and titrated on Vero 2-2 cells50 ...
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bioRxiv - Genomics 2020Quote: ... endometrium tissues fibroblast cells were transfected with a Sendai virus reprogramming kit (Life Technologies, A16517). The transfected cells were then plated onto Matrigel-coated culture dishes according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: Abelson virus-transformed pre-B cell lines were maintained in DMEM (Thermo Fisher #11960-077) supplemented with 10% fetal bovine serum ...
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bioRxiv - Molecular Biology 2023Quote: ... Virus pellet and cell RNA was isolated using TRIzol according to the manufacturer’s protocol (Ambion) and DNase treated ...
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bioRxiv - Microbiology 2023Quote: ... Virus-depleted and concentrated cell SN (in PBS) was added to anti-PA (Thermo Fisher) coated beads and incubated overnight ...
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bioRxiv - Cell Biology 2020Quote: ... centrifuged at approximately 250 × g for 5 min at 4 °C and resuspended in thawed Geltrex™ LDEV ([lactose dehydrogenase elevating virus]-Free Reduced Growth Factor Basement Membrane Matrix; Gibco). Two 15-μl drops of the matrix-cell suspension were pipetted into wells of a Nunc™ 24-well-Nuclon Delta-treated plate (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: We diluted freshly purified virus samples in DPBS and combined three volumes of the virus with one volume of NuPAGE LDS Sample Buffer (4×; Thermo Fisher) containing 100 mM dithiothreitol ...
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bioRxiv - Microbiology 2022Quote: ... Foci development was stopped by fixation with 4% formaldehyde and foci were then stained using a mouse-anti-NS1 antibody (1A5) (gift from Jacob Schlesinger, Rochester University) and a horseradish peroxidase (HRP) conjugated secondary anti-mouse antibody (ThermoFisher 31430). The foci were visualized by diaminobenzidine (DAB ...
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bioRxiv - Microbiology 2023Quote: ... PA (1:250), PB2 (1:250), NS1 (1:250, PA5-32243 and NS2 (1:250, PA5-32234) were all purchased from Thermo Scientific, Rockford ...
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bioRxiv - Biochemistry 2024Quote: ... were infected with the virus at a cell density of 4.0 × 106 cells per milliliter in Sf900 II medium (Gibco), and grown for 48 h at 27 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... then transferred into Grace’s Insect Medium containing 0.25mg/ml BrdU (Invitrogen B23151) and incubated at 25°C for 90 minutes ...
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bioRxiv - Molecular Biology 2019Quote: ... larval brains were dissected in insect media (HyClone SFX; Thermo Fisher Scientific), incubated in 0.5% sodium citrate ...
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bioRxiv - Genomics 2023Quote: ... All cultures were grown in supplemented Grace’s Insect Media (ThermoFisher Scientific #11605094) with 5% fetal bovine serum added (ThermoFisher Scientific #10437010 ...
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Pathogenic CD8 T cell responses are driven by neutrophil-mediated hypoxia in cutaneous leishmaniasisbioRxiv - Immunology 2023Quote: ... braziliensis (MHOM/BR/01/BA788) were grown in Schneider’s insect medium (GIBCO) supplemented with 20% heat-inactivated FBS (Sigma ...
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bioRxiv - Immunology 2021Quote: ... T2 target cells and Epstein-Barr Virus-transformed B lymphoblastoid cell lines (EBV-LCLs) cells were cultured in RPMI-1640 (Thermo Fisher Scientific) supplemented with 10% FCS and 1% penicillin/streptomycin (#P433 ...
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bioRxiv - Cancer Biology 2019Quote: ... followed by 3–4-hour incubation at 4°C with protein A/G agarose (20421, Invitrogen). The beads were then collected for western blot detection ...
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bioRxiv - Physiology 2023Quote: ... 0.5 mg protein was precleared for 30 minutes at 4°C with 4% Agarose beads (ThermoFisher). To immunoprecipitate RYR1 ...
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bioRxiv - Microbiology 2023Quote: ... Mice were anesthetized with ketamine-xylazine and infected intranasally with 5,000 PFU of the non-recombinant virus or 1,000 PFU of the recombinant virus in 50 μl of Dulbecco’s Modified Eagle Medium (DMEM) (Gibco). For antibody treatment ...
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bioRxiv - Immunology 2024Quote: ... Infection was initiated by adding 100 µl of virus inoculum containing 100.000 PFU (MOI ∼0.5-1) in virus absorption medium (Gibco OptiPro Serum free medium + 1:100 Glutamax + 10 mM HEPES ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 4 °C) and the total protein concentration determined by BCA protein assay (Pierce BCA Protein Assay Kit, Thermo Scientific, 23225) following manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were separated by NuPAGE 4%–12%gradient gels (Invitrogen), transferred onto PVDF membranes (Immobilon®-P ...
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bioRxiv - Bioengineering 2022Quote: ... 0.36 pM Recombinant Human Bone Morphogenetic Protein 4 (BMP4, Gibco), and 0.08 pM Activin A (Fisher Scientific) ...
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bioRxiv - Neuroscience 2019Quote: ... Proteins were separated on 4-12% Bis-Tris Gel (Invitrogen), and then transferred to PVDF membranes and probed with the indicated antibodies ...
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bioRxiv - Immunology 2021Quote: ... Proteins were separated on 4-12% Bis-Tris Gels (Invitrogen) and transferred to PVDF membrane (Biorad) ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were resolved on NuPAGE 4%-12% gradient gels (Invitrogen) or self-made 8% gels ...
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bioRxiv - Cancer Biology 2020Quote: ... NuPAGE 4-12% Bis-Tris Midi protein gels (Invitrogen # WG1402A) were used for western blot ...
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bioRxiv - Cancer Biology 2020Quote: Proteins were separated on 4–12% polyacrylamide gels (Life Technologies), transferred to nitrocellulose membranes ...
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bioRxiv - Molecular Biology 2019Quote: ... separated in NuPAGE 4% - 12% Bis-Tris protein gels (ThermoFisher), and analyzed by coomassie blue staining ...
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bioRxiv - Physiology 2020Quote: ... Protein lysates were separated using 4-12% SDS-PAGE (Invitrogen) and transferred to nitrocellulose membranes (Bio-Rad) ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were resolved on a NuPAGE 4-12% gels (Invitrogen) and transferred onto polyvinylidene fluoride membrane (EMD Millipore) ...
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bioRxiv - Immunology 2020Quote: ... Proteins were loaded onto 4-20% SDS–PAGE gels (Invitrogen), and after electrophoretic separation ...