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Citations for Thermo Fisher :
251 - 300 of 10000+ citations for Dengue Virus Serotype 4 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... baculovirus bearing pFBDM-ATG7-ATG10-ATG12-StrepII2x-ATG5-ATG16L1 was used to infect High Five insect cells (Invitrogen). Cells were lysed by sonication and clarified lysate was loaded onto a Strep-Tactin column (QIAGEN) ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell lysates (15-20 ug of protein) were resolved in 4%-12% Bis-Tris gels (Invitrogen) and transferred to PVDF membranes using wet transfer ...
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bioRxiv - Cancer Biology 2020Quote: ... The cell extracts were separated by SDS-PAGE (NuPAGE 4-12% Bis-Tris protein Gels, Thermofisher), followed by transfer to nitrocellulose membrane using wet transfer at 30 V overnight ...
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bioRxiv - Immunology 2020Quote: Proteins derived from cell lysates were separated on SDS-PAGE gel (4 to 12%) (Novex, Invitrogen) with 2-(N-morpholino ...
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bioRxiv - Microbiology 2019Quote: ... The purified virus was quantified using the bicinchoninic acid (BCA) protein assay kit (ThermoFisher Scientific, Waltham, MA, USA) following the manufacturer’s instructions.
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bioRxiv - Genomics 2021Quote: ... insects were immersed in Trizol reagent (Thermo Fisher Scientific, USA) and conserved at -80 ℃ until RNA extraction ...
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bioRxiv - Molecular Biology 2019Quote: ... were grown at 27 °C in Grace’s Insect Medium (Gibco) with 10% FBS.
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bioRxiv - Developmental Biology 2019Quote: Ovaries were hand-dissected in Grace’s Insect Medium (Life Technologies) from flies fed for 3 days with wet yeast paste ...
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bioRxiv - Developmental Biology 2022Quote: Ovaries from flies were dissected in Grace’s Insect Medium (Gibco) and then fixed in 4% formaldehyde (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... Insects were stored in RNAlater (ThermoFisher Scientific, Waltham, MA, USA) or 200 proof ethanol and kept at −20°C prior to processing.
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bioRxiv - Neuroscience 2023Quote: ... twice rinsed in S2 media (Schneider’s Insect Medium, Fisher Scientific), and dissected in cold S2 media on a 9-well glass plate coated with silicone (Sylgard 184 ...
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bioRxiv - Physiology 2023Quote: Ovaries and carcasses were dissected in Grace’s Insect Medium (Gibco), fixed ...
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bioRxiv - Genetics 2023Quote: Ovaries from flies were dissected in Grace’s Insect Medium (Gibco) and then fixed in 4 % formaldehyde (Sigma) ...
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bioRxiv - Bioengineering 2022Quote: ... Human BMP-4 Recombinant Protein (Gibco, Catalog # PHC9534). For cell culture of iPSCs on niches ...
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bioRxiv - Molecular Biology 2020Quote: ... Eighteen million S2 cells were plated to 10 cm-dishes in 12 ml of cell culture media (HyClone SFX-Insect cell culture media with 10% of heat-inactivated FBS [Thermo Fisher Scientific]) one day before experiment ...
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bioRxiv - Biochemistry 2023Quote: Insect cell derived recombinant tag-less eIF2A was expressed in ExpiSf9 cells using the ExpiSf Expression System Starter Kit (Thermo Fisher # A38841) following the manufacturer’s protocol and adapting the IMPACT (Intein Mediated Purification with an Affinity Chitin-binding Tag ...
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bioRxiv - Genetics 2020Quote: ... Protein samples were separated by 4-12% Bis-Tris Protein Gels (Thermo Fisher) and transferred on Amersham™ Protran™ 0.45µm Nitrocellulose (GE-healthcare ...
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bioRxiv - Immunology 2022Quote: ... The proteins were separated by NuPAGE 4-12% Protein Gels (Thermo Fisher Scientific), transferred to PVDF membranes by using the iBlot Gel Transfer Device (Thermo Fisher Scientific) ...
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bioRxiv - Physiology 2021Quote: ... Proteins were separated on 4-12% Bis-Tris protein gels (NuPAGE, Life Technologies) and transferred onto nitrocellulose membranes ...
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bioRxiv - Immunology 2021Quote: Protein lysates were loaded onto 4-12% BIS-Tris Protein gels (NP0336BOX, ThermoFisher) for electrophoresis followed by transfer onto a PVDF membrane (MILIPVH00010 ...
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bioRxiv - Systems Biology 2022Quote: Proteins were separated on NuPAGE 4-12% Bis-Tris protein gels (ThermoFisher Scientific) in MOPS SDS Running Buffer at 160 V for 1 h at room temperature ...
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bioRxiv - Physiology 2023Quote: Protein was separated on NuPAGE 4-12% Bis-Tris protein gels (ThermoFisher Scientific) in MOPS SDS Running Buffer at 160 V for 1 h at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... 4 x TaqMan® Fast Virus 1-Step Master Mix made up with molecular-grade nuclease free water (Ambion) to a final volume of 15 μl ...
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bioRxiv - Cancer Biology 2022Quote: ... the cloned plasmids were transiently transfected into HEK293T cells with packaging plasmid psPAX2 and envelope plasmid pMD2.G using Lipofectamine 3000 transfection reagent (Invitrogen) based on the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... 1.5 μg psPAX2 packaging plasmid and 500 ng pMD2.G envelope plasmid were co-transfected in HEK293 FT cells in 10-cm dishes using Lipofectamine (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... the packaging construct pCMVD8.9 and the envelope coding plasmid pCMV-VSVG (from Dr. Soosan Ghazizadeh, SUNY Stony Brook) into 293T cells using lipofectamine 2000 (Invitrogen). Supernatant containing the virus was collected after 72 hours and filtered through a 0.22 micron PES (Millipore ...
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bioRxiv - Microbiology 2022Quote: ... Viral stock was generated by co-transfecting HEK 293T cells with 10:1 ratio of HIVGKO plasmid and a plasmid encoding VSV-G envelope using Lipofectamine 3000 reagent (Invitrogen). Culture supernatant was harvested 48hrs post-transfection ...
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Zero-shot learning enables instant denoising and super-resolution in optical fluorescence microscopybioRxiv - Bioengineering 2023Quote: ... together with psPAX2 packaging and pMD2.G envelope plasmid DNA were co-transfected to HEK293T cells using Lipofectamine 3000 (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... The sgRNA was ordered as modified synthetic sgRNA from Synthego and delivered to HeLa cells by electroporating 200,000 cells with 4 ug TrueCut Cas9 protein (ThermoFisher Scientific) complexed with 180 pmol GBP1 sgRNA using a Neon system (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Loss of virus was monitored by staining with anti-Sendai virus antibody (Invitrogen, cat #14649482).
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bioRxiv - Biophysics 2022Quote: ... and pCMV-VSV-G envelope plasmids using Lipofectamine 2000 (Thermo Fisher Scientific) as recommended by the manufacturer ...
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bioRxiv - Cell Biology 2020Quote: Constructs for insect cell expression were transfected and expressed in SF9 cells using the Bac-to-Bac® expression system (Thermo Fisher Scientific). Expression was carried out for 72 hours at 27°C ...
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bioRxiv - Molecular Biology 2020Quote: ... and the virus was produced in HEK293A cells as described by the manufacturer (Invitrogen). Immature brown adipocytes were treated with the viruses on day 2.
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bioRxiv - Microbiology 2022Quote: ... Total RNA was extracted from mock or virus infected cells using Trizol reagent (Invitrogen) at 2 and 4 hours post infection ...
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bioRxiv - Plant Biology 2022Quote: ... Virus amplification was carried out in Spodoptera frugiperda Sf9 cells (Geneart, Thermo Fisher Scientific) and was used to infect Trichoplusia ni Tnao38 cells (103 ...
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bioRxiv - Immunology 2023Quote: ... Concentrated virus was then incubated using 1:2000 DiD-Cell labelling solution (ThermoFisher V22887) for 20 mins at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... Virus pellets and cell lysates were dissolved in radioimmunoprecipitation assay (RIPA) buffer (Thermo Scientific) containing protease inhibitors ...
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bioRxiv - Developmental Biology 2023Quote: IPS cells were derived from skin fibroblasts using Cytotune Sendai virus kit (Thermofisher, France) and cultured on MEF in KO- DMEM supplemented with KO-SR (Thermofisher ...
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bioRxiv - Cell Biology 2024Quote: Elephas maximus endothelial and epithelial cells were transduced with Sendai virus (Thermofisher Scientific, A16517) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Abelson virus-transformed pre-B cells were maintained in DMEM (Thermo Fisher, 11960-077) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2020Quote: ... and the supernatant was incubated with prewashed Ni-NTA resin (1.25 ml slurry/l insect cells; Thermo Fisher Scientific) for 1 h at 4°C ...
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bioRxiv - Plant Biology 2020Quote: ... The RDR2 and RDR2-asm genes were expressed in insect cells as BaculoDirect™ expression vectors (Thermo Fisher Scientific). Recombinant proteins were affinity purified using nickel-nitrilotriacetic acid (Ni-NTA ...
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bioRxiv - Biophysics 2020Quote: ... Insect Sf9 cells were maintained as a shaking culture in Sf-900II serum-free medium (SFM) (Thermo Fisher Scientific), or alternatively in ESF media (Expression Systems ...
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bioRxiv - Molecular Biology 2022Quote: His6-tagged human Gβ1 and Gγ2 with C68S mutation was expressed in insect cell using the Bac-to-Bac Baculovirus expression system (Invitrogen) and purified as previously described (38) ...
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bioRxiv - Immunology 2022Quote: ... Axenic amastigotes were generated by culturing stationary-phase promastigotes at 32°C in Grace’s insect cell culture medium (Invitrogen), pH 5.2 ...
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bioRxiv - Cell Biology 2022Quote: All recombinant condensin complexes were expressed using insect cell strains from the Bac-to- Bac Baculovirus Expression System (ThermoFisher) as previously described (Kinoshita et al. ...
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bioRxiv - Microbiology 2019Quote: The Spodoptera frugiperda (Sf21) insect cell line was cultured in Gibco® Grace’s Medium (1X) (Thermo Fisher Scientific, Waltham) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Plant Biology 2019Quote: Full-length ELF3 (TAIR At2g25930.1) was cloned into the pACEBac1(49) vector and produced in Sf21 insect cells (Invitrogen) using the baculovirus expression system.
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bioRxiv - Microbiology 2020Quote: ... frugiperda) IPLB-Sf21-AE clonal isolate 9 (Sf9) insect cells were cultured at 27°C in Grace’s medium (Gibco) containing 10% fetal bovine serum (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... and CD94/NKG2C for SPR were produced using a baculovirus expression system with High Five (Hi5) insect cells (ThermoFisher). Individual constructs were cloned into pAcGP67a vectors ...