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Citations for Thermo Fisher :
251 - 300 of 10000+ citations for Dengue Virus Serotype 4 Envelope Protein HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: We diluted freshly purified virus samples in DPBS and combined three volumes of the virus with one volume of NuPAGE LDS Sample Buffer (4×; Thermo Fisher) containing 100 mM dithiothreitol ...
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bioRxiv - Cancer Biology 2019Quote: ... followed by 3–4-hour incubation at 4°C with protein A/G agarose (20421, Invitrogen). The beads were then collected for western blot detection ...
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bioRxiv - Physiology 2023Quote: ... 0.5 mg protein was precleared for 30 minutes at 4°C with 4% Agarose beads (ThermoFisher). To immunoprecipitate RYR1 ...
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bioRxiv - Immunology 2021Quote: ... Virus was titrated by adding serial dilutions of virus supernatant (8 replicates) on VeroE6 cells in DMEM (Gibco) containing 2% FBS ...
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bioRxiv - Microbiology 2023Quote: ... Mice were anesthetized with ketamine-xylazine and infected intranasally with 5,000 PFU of the non-recombinant virus or 1,000 PFU of the recombinant virus in 50 μl of Dulbecco’s Modified Eagle Medium (DMEM) (Gibco). For antibody treatment ...
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bioRxiv - Immunology 2024Quote: ... Infection was initiated by adding 100 µl of virus inoculum containing 100.000 PFU (MOI ∼0.5-1) in virus absorption medium (Gibco OptiPro Serum free medium + 1:100 Glutamax + 10 mM HEPES ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 4 °C) and the total protein concentration determined by BCA protein assay (Pierce BCA Protein Assay Kit, Thermo Scientific, 23225) following manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... envelope plasmid pIP/VSV-G (5μg) (ViraPower™ Lentiviral Packaging Mix, Thermo Fisher Scientific) and 5μg lentiviral expression constructs shRNA (pLKO.1 Mission shRNA DNA clone ...
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bioRxiv - Cell Biology 2020Quote: ... 2.5µg psPAX2 packaging plasmid and 1µg pMD2.G envelope plasmid using Lipofectamine 2000 (Invitrogen). Lentiviral supernatant was collected ...
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bioRxiv - Microbiology 2022Quote: ... Cell envelope samples were vitrified using a Vitrobot plunge-freezing machine (Mark IV, ThermoFisher) at room temperature (blot force 2 ...
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bioRxiv - Molecular Biology 2020Quote: ... the cell envelope was stained with wheat germ agglutinin-AlexaFluor488 (WGA-AF488, Life Technologies), which was added to the loading well to a final concentration of 10 μg/mL prior to loading cells into the imaging chamber ...
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bioRxiv - Molecular Biology 2020Quote: ... pMD2-VSVG (envelope vector) and pCDH plasmids co-transfection using Lipofectamine 2000 (#11668019, Invitrogen). Medium containing virus was harvested 48 h after transfection and filtered by 0.45 μm millipore filter ...
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bioRxiv - Genomics 2022Quote: ... psPAX2 packaging plasmids and pMD2.G envelope plasmids using Lipofectamine 2000 (Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: CH848 SOSIP gp140 envelope production was performed with Freestyle293 cells (ThermoFisher Cat No. R79007). On the day of transfection ...
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bioRxiv - Cancer Biology 2023Quote: ... envelope plasmid pIP/VSV-G (5 µg) (ViraPowerTM Lentiviral Packaging Mix, Thermo Fisher Scientific), 5 µg of lentiviral expression construct shRNA (pLKO.1 Mission shRNA DNA clone ...
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bioRxiv - Cancer Biology 2023Quote: ... and the envelope vector pVSV-G using Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... 600 ng VSV-G envelope plasmid and 6 μL of Lipofectamine 2000 (Thermo Scientific). Supernatants were collected at 48 and 72 h post-transfection and clarified using 0.45 μm filters ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were separated by NuPAGE 4%–12%gradient gels (Invitrogen), transferred onto PVDF membranes (Immobilon®-P ...
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bioRxiv - Bioengineering 2022Quote: ... 0.36 pM Recombinant Human Bone Morphogenetic Protein 4 (BMP4, Gibco), and 0.08 pM Activin A (Fisher Scientific) ...
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bioRxiv - Neuroscience 2019Quote: ... Proteins were separated on 4-12% Bis-Tris Gel (Invitrogen), and then transferred to PVDF membranes and probed with the indicated antibodies ...
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bioRxiv - Immunology 2021Quote: ... Proteins were separated on 4-12% Bis-Tris Gels (Invitrogen) and transferred to PVDF membrane (Biorad) ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were resolved on NuPAGE 4%-12% gradient gels (Invitrogen) or self-made 8% gels ...
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bioRxiv - Cancer Biology 2020Quote: ... NuPAGE 4-12% Bis-Tris Midi protein gels (Invitrogen # WG1402A) were used for western blot ...
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bioRxiv - Cancer Biology 2020Quote: Proteins were separated on 4–12% polyacrylamide gels (Life Technologies), transferred to nitrocellulose membranes ...
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bioRxiv - Molecular Biology 2019Quote: ... separated in NuPAGE 4% - 12% Bis-Tris protein gels (ThermoFisher), and analyzed by coomassie blue staining ...
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bioRxiv - Physiology 2020Quote: ... Protein lysates were separated using 4-12% SDS-PAGE (Invitrogen) and transferred to nitrocellulose membranes (Bio-Rad) ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were resolved on a NuPAGE 4-12% gels (Invitrogen) and transferred onto polyvinylidene fluoride membrane (EMD Millipore) ...
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bioRxiv - Immunology 2020Quote: ... Proteins were loaded onto 4-20% SDS–PAGE gels (Invitrogen), and after electrophoretic separation ...
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bioRxiv - Cell Biology 2019Quote: ... Protein concentration was determined using a Qubit 4 fluorometer (Invitrogen). To determine Muc5B presence ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... specifically bound proteins were resolved on 4-12% gel (Invitrogen) and detected by Coomassie staining ...
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bioRxiv - Microbiology 2019Quote: ... Protein concentration was determined using a Qubit 4 fluorometer (Invitrogen).
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bioRxiv - Microbiology 2020Quote: ... Proteins were separated in either 4–12% NuPAGE gels (ThermoFisher) or in custom 10% polyacrylamide gels and transferred to nitrocellulose membrane ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were resolved in 4–12% Bis-Tris gels (Invitrogen) at 100 V for 2.5 h and then transferred to a nitrocellulose membrane (Amersham Protran 0.2 µm NC) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Proteins were separated on 4-12% SDS-PAGE gels (Invitrogen) and transferred to nitrocellulose membranes ...
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bioRxiv - Neuroscience 2023Quote: ... Protein lysates were analyzed on 4–12% NuPAGE gels (Invitrogen), transferred to PVDF membranes ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were separated in NuPage BisTris 4-12% gels (Invitrogen), then transferred onto PVDF membranes ...
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bioRxiv - Biochemistry 2023Quote: Protein samples were resolved on a 4-20% acrylamide (ThermoFisher) pre-cast Tris-Glycine gels and stained with Coomassie Brilliant Blue stain or transferred for western blotting analyses onto 0.4 µm nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were resolved using SDS-PAGE (Invitrogen NuPage 4-12%), transferred onto nitrocellulose membrane (Amersham ...
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bioRxiv - Pathology 2024Quote: ... Proteins were separated on 4-12% Bis-Tris gels (Invitrogen) and transferred onto a Polyvinylidene difluoride (PVDF ...
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bioRxiv - Cancer Biology 2024Quote: ... or NuPAGE Bis-Tris Protein Gel (4-12%) (Thermo Fisher) and probed with primary antibodies as indicated ...
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bioRxiv - Molecular Biology 2023Quote: ... a 4-12% NuPAGETM Bis-Tris protein gel (Thermo Fisher) with the MOPS running buffer was used for electrophoresis ...
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... Pelleted virus was resolved in PBS (Gibco) and stored at −80°C until further usage ...
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bioRxiv - Immunology 2019Quote: ... murine leukemia virus reverse transcriptase (RT; Invitrogen), and random hexamers (Applied Biosystems) ...
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bioRxiv - Neuroscience 2021Quote: ... Fibroblasts were reprogrammed using Sendai virus (Invitrogen) in feeder-free conditions on Matrigel (Corning ...
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bioRxiv - Cell Biology 2023Quote: ... and EmGFP reporter Sendai Virus (A16519, Invitrogen) were part of the Cytotune -iPS 2.0 Sendai Reprogramming kit and EmGFP Sendai Fluorescence Reporter kit ...
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bioRxiv - Immunology 2024Quote: ... in virus growth medium (1X MEM [Gibco 11095080] ...
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bioRxiv - Microbiology 2022Quote: ... The levels of RNA corresponding to the N protein-encoding gene of SARS-CoV-2 were measured using the TaqMan Fast Virus 1-step Master Mix (Thermo Scientific). Each 20-μL reaction mixture contained 5.0 μL of 4× TaqMan Fast Virus 1-Step Master Mix ...
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bioRxiv - Microbiology 2019Quote: ... The viral N-protein was labeled using a primary anti-Tula virus antibody(12) and a secondary anti-rabbit FITC conjugate (Invitrogen, USA). The viral Gc protein was stained using a mouse monoclonal antibody (#H1808-60B ...
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bioRxiv - Bioengineering 2023Quote: ... The levels of RNA corresponding to the N protein-encoding gene of SARS-CoV-2 were measured using the TaqMan Fast Virus 1-step Master Mix (Thermo Scientific). Each 20-μL reaction mixture contained 5.0 μL of 4× TaqMan Fast Virus 1-Step Master Mix ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein containing fractions were checked for contaminants on NuPAGE 4-12 % Bis – Tris Protein Gels (Invitrogen), pulled ...