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Citations for Thermo Fisher :
101 - 150 of 4234 citations for Dehydroepiandrosterone sulfate sodium salt since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: Purified rFIX samples were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using a 4-12% Bis-Tris Bolt acrylamide gel (Invitrogen) in 1x MES running buffer (Invitrogen) ...
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bioRxiv - Cancer Biology 2021Quote: ... Twenty μL of clarified lysates were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using 4-12% or 10% polyacrylamide Bis-Tris gels (Invitrogen) and transferred onto Immobilon FL PVDF or P PVDF membranes (Millipore ...
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bioRxiv - Cell Biology 2021Quote: ... Protein samples were loaded for each group in each well and run on 8-16% sodium dodecyl sulfate–polyacrylamide gel (ThermoFisher) for electrophoresis and then transferred to the PVDF membrane ...
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bioRxiv - Immunology 2022Quote: ... Proteins were separated by size on a 12% sodium dodecyl sulfate polyacrylamide gel and blotted onto a 0.2 µm PVDF membrane (Thermo Scientific) by semi-dry blotting (BioRad) ...
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bioRxiv - Synthetic Biology 2022Quote: ... Samples of elution fractions were taken to perform sodium dodecyl sulfate polyacrylamide agarose gel electrophoresis (SDS-PAGE) (Bolt 4-12% Bis-Tris Plus, Invitrogen) at 180 V for 30 min in 1× MES buffer (Novex ...
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bioRxiv - Cancer Biology 2022Quote: ... and 0.06% Bromophenol Blue) at 98°C for 5 minutes and separated on 12% sodium dodecyl sulfate (SDS)-polyacrylamide gels (Invitrogen). Proteins were transferred to nitrocellulose membranes (Millipore ...
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bioRxiv - Immunology 2020Quote: ... Samples containing 30 µg/µl of cell lysate proteins per lane were resolved on SDS-PAGE (10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis) along with pre-stained protein ladder and transferred onto PVDF membranes (Invitrogen). The transferred membranes were blocked for 2 hours using 5% BSA in TBST (25 mm Tris-HCl ...
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bioRxiv - Biophysics 2021Quote: The purified proteins were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using 4-12% Bis-Tris NuPAGE gels (Invitrogen). Prior to SDS-PAGE analysis ...
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bioRxiv - Cell Biology 2020Quote: A total of 20 μg of protein was processed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with a 4-12% Bis-Tris Mini-gel (Invitrogen) and the 3-(N-morpholino ...
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bioRxiv - Cell Biology 2020Quote: ... of cell lysate proteins per lane were resolved on SDS-PAGE (8% sodium dodecyl sulfate-polyacrylamide gel electrophoresis) along with pre-stained protein ladder and transferred onto PVDF membranes (Invitrogen). The transferred membranes were blocked for 1 hour using 5% BSA in TBST (25 mm Tris-HCl ...
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bioRxiv - Cancer Biology 2022Quote: ... Fourteen μL of clarified lysates were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using 10% polyacrylamide Bis-Tris gels (Invitrogen) and transferred onto Immobilon FL PVDF membranes (Millipore ...
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bioRxiv - Cell Biology 2023Quote: ... slides were thawed at room temperature for 1 hr and then placed in 15mls of 1% UltraPure Sodium Dodecyl Sulfate (SDS) solution (cat# 15553035; Invitrogen) for 5-10 minutes with agitation (40 rpm) ...
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bioRxiv - Neuroscience 2023Quote: ... were conducted to remove trace PFA followed by 5 min antigen retrieval with SDS (1% Sodium Dodecyl Sulfate; Fisher Scientific) in PBS ...
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bioRxiv - Immunology 2023Quote: ... the samples were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini Gel Tank electrophoresis system (Invitrogen). Proteins were denatured in a loading buffer (4X LDS sample buffer +β-mercaptoethanol ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were boiled for 5 min and subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) on either 10% gels (cast freshly) or 4-20% gradient gels (Invitrogen). Proteins were transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Cell Biology 2023Quote: ... 0.1% sodium dodecyl sulfate) containing protease and phosphatase inhibitors (Pierce Protease Inhibitor Mini Tablet, EDTA-free, Thermo Scientific; PhosSTOP, Roche) for 45 minutes at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Equal concentrations of protein were prepared under reducing conditions and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) (4–20% Tris Glycine 1.0mm Mini Protein Gels from Invitrogen XP04200BOX). Gels were transferred to PVDF membranes using the iBlot™ 2 transfer stacks (ThermoFisher IB24002) ...
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bioRxiv - Bioengineering 2023Quote: ... Protein gels were used to check for the presence and purity of protein and run using sodium dodecyl sulfate polyacrylamide gradient gels (NuPage 4−12% Bis-Tris gel from Invitrogen) using the Precision Plus Dual Color protein standard (BioRad ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 µg of each sample were subjected to Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS/PAGE) using precast 10% Bis-Tris NuPage Gels (Invitrogen), transferred onto PVDF membranes ...
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bioRxiv - Molecular Biology 2023Quote: ... 10-20µg of protein was loaded per well and run on a 4-12% bis-tris precast sodium-dodecyl sulfate page gel (NP0323BOX, Invitrogen). Semi-dry transfer was performed using the TransBlot Turbo transfer system (1704150 ...
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bioRxiv - Developmental Biology 2023Quote: Protein extracts were separated by 10% sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto nitrocellulose membranes (Invitrogen). Blocking and antibody incubations were performed in 5% bovine serum albumin ...
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bioRxiv - Cell Biology 2023Quote: Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed with 8% or 10% acrylamide gels and BOLT MES (Invitrogen) or Tris-glycine SDS running buffer ...
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bioRxiv - Neuroscience 2023Quote: ... were conducted to remove trace PFA followed by 5 min antigen retrieval with SDS (1% Sodium Dodecyl Sulfate; Fisher Scientific) in PBS ...
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bioRxiv - Cell Biology 2019Quote: ... Samples with equal protein amounts were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) using standard methods (89) and electroblotted onto polyvinylidene fluoride (PVDF) membrane (ThermoFisher, 88518). After blocking in Tris-buffered saline with 0.1% Tween (TBST ...
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bioRxiv - Microbiology 2021Quote: ... Samples were diluted in 6X Laemmli sample buffer and resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose membranes (Thermo Scientific). Blots were probed with the indicated primary antibodies and with horseradish peroxidase (HRP ...
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bioRxiv - Neuroscience 2021Quote: ... 20 μg of each sample was separated in 8% sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membranes (45-004-110, Fisher Scientific) in ice-cold transfer buffer (25 mM Tris-base ...
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bioRxiv - Systems Biology 2020Quote: ... cell pellets were lysed via bead beating in presence of 2% sodium dodecyl sulfate (SDS) and an aliquot from each sample was digested using Pierce MS grade trypsin (Thermo Fisher), labeled with TMT 10plex isobaric tags (Thermo Fisher) ...
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bioRxiv - Bioengineering 2021Quote: ... once with a 1% SDS-TRIS pH 11 solution (5% v/v of 20% sodium dodecyl sulfate #05030, from Sigma Aldrich and 2.4 % w/v TRIS base PBP151-500 from Fisher Scientific in reverse osmosis water ...
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bioRxiv - Microbiology 2020Quote: ... Enriched virus was inactivated with 2% sodium dodecyl sulfate (SDS) and protein concentration determined with a Pierce BCA protein assay kit (Thermo Scientific) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Protein lysates of HAP1 and HeLa cells were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) using 4-12% gradient precast polyacrylamide gels (Life Technologies). The proteins separated by SDS-PAGE were transferred onto a nitrocellulose membrane using a Mini-Protean® Tetra Cell device ...
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bioRxiv - Microbiology 2019Quote: Viral and cell lysates were resuspended in sodium dodecyl sulfate (SDS) sample buffer and separated by electrophoresis on Bolt 4-12% Bis-Tris Plus gels (Life Technologies), blotted onto nitrocellulose membranes and probed overnight at 4°C with the following antibodies in Odyssey Blocking Buffer (LI-COR) ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were then separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) using NuPAGE Bis-Tris gels (Thermo Fisher Scientic) and transferred on nitrocellulose membranes (iBlot 2® gel transfer Stacks Nitrocellulose system from Invitrogen) ...
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bioRxiv - Molecular Biology 2022Quote: ... were resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) before transferring onto 0.45 μm nitrocellulose membrane (Thermo Scientific™) with a Hoefer TE77X semi-dry transfer unit at 20V for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... magnetically isolated for a final time and then boiled in Laemmli buffer (62.5 mM Tris-HCl, 10% glycerol, 2% sodium dodecyl sulfate and 0.01% bromophenol blue (B392, Fisher Scientific, Pittsburgh, PA) for 5 minutes to release antibody ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Cells were lysed in Laemmli buffer and separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred to a polyvinylidene fluoride membrane (ThermoFisher, 88518). Membranes were blocked with 5% milk in TBST for 1h ...
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bioRxiv - Microbiology 2022Quote: ... Equal protein loads were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and proteins were transferred to nitrocellulose membranes (Thermo Scientific). For protein detection the following primary antibodies were used at the indicated dilutions ...
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bioRxiv - Neuroscience 2023Quote: ... the samples were embedded in a thin polyacrylamide gel and were then treated with a digestion buffer of 2% v/v sodium dodecyl sulfate (SDS; ThermoFisher, AM9823), 0.5% v/v Triton X-100 (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... Equal protein loads were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and proteins were transferred to nitrocellulose membranes (Thermo Scientific). For protein detection the following primary antibodies were used at the indicated dilutions ...
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bioRxiv - Immunology 2023Quote: ... Integrity and purity of purified proteins were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis and Coomassie G-250 staining (ThermoFisher Scientific). Purified proteins were stored at –80°C until use.
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bioRxiv - Biochemistry 2023Quote: ... The purity of the ATAD2/B bromodomain was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels stained with GelCode Blue Safe protein stain (Thermo Scientific) (24,25).
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bioRxiv - Neuroscience 2023Quote: ... 15 µg of total protein was resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using a 10-20% tris-glycine gradient (Invitrogen, XP10205BOX), transferred to a PVDF membrane using the Trans-Blot Turbo Transfer System (BioRad ...
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bioRxiv - Cell Biology 2023Quote: ... lysates were separated by sodium dodecyl sulfate/polyacrylamide (SDS/PAGE) gel electrophoresis on 1 mm NuPAGE 3 – 8% gradient Tris-Acetate gels (Thermo Fisher) using Tris-Acetate SDS running buffer and transferred to nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... Cell pellets were lysed in 10% sodium dodecyl sulfate (SDS) containing 100 mM triethylammonium bicarbonate (TEAB) with Pierce protease inhibitor (ThermoFisher Scientific) supplemented ...
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bioRxiv - Biophysics 2023Quote: ... Proteins and lipids in the SEC fractions were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) on pre-cast 4-12% gradient gel (Life technologies) and by LC-MS/MS ...
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bioRxiv - Physiology 2024Quote: ... Twenty-five µg of protein were separated by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose membranes (Fisher Scientific). Western blot analysis was performed with the following antibodies ...
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bioRxiv - Biochemistry 2020Quote: ... ammonium sulfate (Fisher Scientific), 2% (w/v ...
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bioRxiv - Synthetic Biology 2020Quote: ... ammonium sulfate (Fisher Scientific), 2% (w/v ...
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bioRxiv - Biophysics 2023Quote: ... ammonium sulfate (Fisher Scientific) (5g/L) ...
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bioRxiv - Microbiology 2024Quote: ... copper sulfate (Fisher Scientific), or diamide (MP Biomedicals ...
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bioRxiv - Immunology 2021Quote: ... and final product purity were assessed on a 15% sodium-dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) using SYPRO Ruby Protein Gel Stain (S12000, Thermo Fisher Scientific). Refolding efficacy was measured by denaturing XCL1 with 20 µM beta-mercaptoethanol (-ME) ...