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Citations for Thermo Fisher :
1 - 50 of 56 citations for Decarboxybiotin alkyne since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... The labelled lysate was then either “clicked” on with TAMRA-alkyne or biotin-alkyne using copper catalyzed azide-alkyne cycloaddition (CuAAC) click chemistry reaction protocol supplied by ThermoFisher. Biotin-labelled samples were then immunoblotted and visualized with Streptavidin-HRP antibody on an iBright™ FL-1500 imaging system (ThermoFisher).
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Nucleocytoplasmic transport rates are regulated by cellular processes that modulate GTP availabilitybioRxiv - Cell Biology 2023Quote: ... and TAMRA alkyne (Invitrogen) according to manufacturer instructions ...
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bioRxiv - Neuroscience 2024Quote: ... and Alexa Fluor 555 alkyne (Alexa Fluor™ 555 Alkyne, Cat No. A20013, Thermo Fisher Scientific).
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bioRxiv - Bioengineering 2021Quote: ... Click-IT™ Fucose Alkyne (Invitrogen), 6-Azidohexanoic Acid Ester (Click Chemistry tools ...
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bioRxiv - Neuroscience 2024Quote: ... 2 μM fluorescent Alexa Fluor 555 alkyne (Alexa Fluor™ 555 Alkyne, Cat No. A20013, Thermo Fisher Scientific), and 200 μM CuSO4 in PBS ...
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Nucleocytoplasmic transport rates are regulated by cellular processes that modulate GTP availabilitybioRxiv - Cell Biology 2023Quote: ... was performed with Tetramethylrhodamine (TAMRA) alkyne (Invitrogen) according to manufacturer’s suggestions ...
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bioRxiv - Developmental Biology 2024Quote: ... and Alkyne Alexa Fluor 488 (Invitrogen A10267) followed by SOX2-V450.
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bioRxiv - Immunology 2023Quote: ... Alexa Fluor 488 Alkyne (Fisher Scientific, cat#A10267) was used in place of Alexa Fluor 555 for FACS analysis.
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bioRxiv - Molecular Biology 2022Quote: ... and Alexa Fluor 594 Alkyne (Thermo Fisher Scientific) in 1 μM final concentration according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... The labelled lysate was then clicked on with biotin-alkyne using copper catalyzed azide-alkyne click chemistry reaction protocol according to manufacturer’s instruction (ThermoFisher Scientific C33372). The biotinylation was detected using IRDye 800CW Streptavidin dye and imaged (Odyssey Image System LICOR).
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bioRxiv - Neuroscience 2023Quote: ... TAMRA-alkyne dye (Invitrogen, 1 mM, 1:5000 dilution), tris-(2-carboxyethyl ...
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bioRxiv - Neuroscience 2024Quote: ... 0.4 µM AlexaFluor 488 Alkyne (Thermo Fisher Scientific, A10267) and 200 µM copper (II ...
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bioRxiv - Cell Biology 2024Quote: ... 62.5 μM biotin-alkyne tag (Thermo Fisher Scientific, B10185) and 83 μg/mL CuBr (prepared by dilution of a fresh 10 mg/mL solution in DMSO) ...
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bioRxiv - Molecular Biology 2020Quote: ... alkyne-Alexa Fluor® 555 were purchased from Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2024Quote: ... a click-chemistry reaction with AF647 sDIBO alkyne (Thermo Fisher) was conducted ...
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bioRxiv - Bioengineering 2021Quote: ... Fluorescently labeled PS were prepared by conjugating AlexaFluor-647 alkyne dye (ThermoFisher) to PS containing 25% N3-PEG-PPS ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 μl alkyne agarose beads (Click-iT™ Protein Enrichment Kit, ThermoFisher Scientific) were washed three times with
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bioRxiv - Cell Biology 2021Quote: ... Azide-alkyne cycloaddition was performed using the Click-iT Cell Reaction Buffer Kit (Invitrogen) with azide conjugated to Alexa Fluor 596 at 5 µM final concentration ...
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bioRxiv - Cell Biology 2023Quote: ... Alexa Fluor 595 Alkyne diluted in Click-iT® Cell Reaction Buffer (Thermo Scientific) at a final concentration of 2.5 mM was conjugated to any incorporated AHA.
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bioRxiv - Biophysics 2021Quote: ... or Click-iT™ Alexa Fluor™ 647 sDIBO Alkyne (FRET acceptor, Thermo Fisher Scientific) with a 1:2 protein-to-dye molar ratio at 4 °C for 2-7 days ...
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bioRxiv - Cell Biology 2024Quote: ... biotin azide (with HPG alkyne) was detected by Streptavidin Alexa Fluor™ 647 conjugate (Invitrogen) and acquired by iBright FL1500 Imaging ...
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bioRxiv - Molecular Biology 2022Quote: ... labeled using 150 molar excess of Click-IT Alexa Fluor 647 sDIBO Alkyne (ThermoFisher Scientific) at room temperature for 60 min.
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bioRxiv - Neuroscience 2022Quote: ... according to the manufacturer’s protocol in order to label the AHA-incorporated proteins with biotin alkyne (ThermoFisher, B10185). The Click reaction samples were incubated on a rotator at 4 °C for 1 h ...
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bioRxiv - Cancer Biology 2023Quote: ... The azide-alkyne reaction was performed using the Click-iT™ Cell Reaction Buffer Kit (Thermo Fisher Scientific) with Alexa Fluor™ 647 Azide (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... washed extensively with PBS pH 7.8 and clicked overnight with Alexa Fluor™ 647 Alkyne (Thermo Fisher, A10278), triazole ligand (Sigma ...
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bioRxiv - Biochemistry 2023Quote: The DIBO labeling solution was prepared by dissolving 0.5 mg of DIBO-alkyne Alexa Fluor 555 dye (ThermoFisher) in dimethyl sulfoxide (DMSO ...
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bioRxiv - Neuroscience 2020Quote: ... then conjugated with an alkyne-biotin compound according to the Click-iT Protein Analysis Detection Kit (Molecular Probes, C33372) protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Rates of AHA incorporation were determined by labeling the cells with Alexa Fluor® 488 DIBO alkyne (Invitrogen C10405), followed by imaging and quantification of the integrated intensity of the fluorophore on a single cell basis as detailed above ...
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Ribonuclease Inhibitor and Angiogenin collaboratively regulate cell-type-specific global translationbioRxiv - Biochemistry 2024Quote: ... and Click-iT reaction was performed using TAMRA alkyne and the Click-iT Protein Reaction Buffer Kit (C10276, Invitrogen) for 30min according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2023Quote: ... an amino acid analog of methionine containing an alkyne moiety that can be biotinylated by click-it chemistry (ThermoFisher), for nascent protein translation labeling followed by SDS-PAGE and HRP-streptavidin Western analysis ...
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bioRxiv - Cancer Biology 2023Quote: ... the azide-alkyne cycloaddition was performed using the Click-iT Cell Reaction Buffer Kit (Life Technologies, Carlsbad, CA, USA) and azide conjugated to Alexa Fluor 555 (Life Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... Biotin was attached to cross-linked RNA duplexes by incubating at 37°C for 1.5 h with 150mM Click-IT Biotin DIBO Alkyne (Life technologies) under constant agitation ...
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bioRxiv - Bioengineering 2021Quote: ... Samples were then reacted for 2 h at RT with the following click reagents: 50 μM biotin-alkyne (ThermoFisher Scientific), 5 mM tris(3-hydroxypropyltriazolylmethyl)amine (THPTA ...
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bioRxiv - Microbiology 2021Quote: ... or 3.8 x 106/ml (Δfut1s) and grown for three days in the presence/absense of 100µM alkyne fucose (Invitrogen C10264), after which parasites were washed in PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... Biotin was attached to cross-linked RNA duplexes by incubating with 150 µM Click-IT Biotin sDIBO Alkyne (Life technologies) under constant agitation at 37 °C for 1.5 hours ...
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bioRxiv - Microbiology 2022Quote: ... The membrane containing the biotin alkyne labeled proteins was incubated in a solution containing 1% BSA with streptavadin-HRP (Invitrogen) for subsequent visualization.
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bioRxiv - Microbiology 2022Quote: ... Click-chemistry reaction for protein detection was performed using biotin alkyne (PEG4 carboxamide-propargyl biotin) according to manufacturer’s instructions using the Click-iT™ Protein Reaction Buffer Kit (Invitrogen). Labeled proteins were separated by SDS-PAGE ...
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bioRxiv - Molecular Biology 2022Quote: ... The copper-catalyzed azide-alkyne cycloaddition reaction was conducted for 18 h on the Versette liquid handling robot (ThermoFisher Scientific) at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... The de-salted azide labeled protein was then labeled with IrPC alkyne (150μM final concentration) using the click-chemistry kit (ThermoFisher: C10276). The sample was incubated for 30 minutes followed by desalting on a Zeba spin column (MWCO 40kDa) ...
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bioRxiv - Biophysics 2023Quote: ... The Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) reaction was then performed to add Azido-Alexa 647 fluorophores (ThermoFisher, C10340) to the F-ara-EdU loci on the chromatin DNA ...
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bioRxiv - Neuroscience 2022Quote: ... Supernatants underwent a cyclo-addition reaction to attach AHA-labelled proteins to alkyne agarose using the Click IT cell reaction buffer kit (#C10269, ThermoFisher Scientific). Alkyne agarose (25 μl per sample ...
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bioRxiv - Cell Biology 2024Quote: ... 200 μg of total protein in a maximum volume of 50 μL was used for the Click-it reaction between Click-iT® HPG alkyne and Biotin Azide (PEG4 carboxamide-6-Azidohexanyl Biotin) (Invitrogen) was performed using Click-iT™ Protein Reaction Buffer Kit (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... The azide group of ADA-DA was labelled (post-fixation) with 5 µg ml−1 Alexa Fluor 488 Alkyne using the Click-iT™ Cell Reaction Buffer Kit (Invitrogen) according to the manufacturer instructions.
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bioRxiv - Microbiology 2020Quote: ... incorporated AHA was detected by labeling 40 μL of each soluble protein fraction with 22.5 μM of TAMRA alkyne in a final volume of 128 μL following the Click-IT® Protein Reaction Buffer Kit recommendations (ThermoFisher, Germany). After methanol/chloroform protein precipitation ...
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bioRxiv - Cell Biology 2020Quote: ... AHA-labeled proteins were detected with 1 μM alkyne-tagged Alexa Fluor-488 using Click-iT cell detection reagents (Thermo Fisher Scientific) for 45 min at room temperature as previously described (3).
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bioRxiv - Neuroscience 2022Quote: ... CuAAC (Copper(I)-catalyzed Azide-Alkyne Cycloaddition) was performed using the Click-iT Cell Reaction Buffer Kit (Thermo Fisher, catalog no. C10269) according to manufacturer instructions ...
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bioRxiv - Cell Biology 2024Quote: ... and the Click-it reaction between Click-iT® HPG alkyne (present in protein and Alexa Fluor™ 555 Azide, Triethylammonium Salt (Invitrogen) was applied using the Click-iT™ Cell Reaction Buffer Kit (Invitrogen) ...
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bioRxiv - Neuroscience 2024Quote: ... Incorporated AHA was detected using Fluorescent Non-canonical Amino Acid Tagging (FUNCAT) via cycloaddition of alkyne-conjugated Alexa Fluor 647 (Thermo Scientific A10278) (Dieterich et al. ...
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bioRxiv - Cancer Biology 2024Quote: ... 200μg of protein was used for click chemistry and was performed using biotin-alkyne and protein reaction buffer kits (Thermo Fisher: C10276). AHA-biotin-alkyne labeled proteins were pulled down with streptavidin beads and the indicated proteins were analyzed by immunoblotting.
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bioRxiv - Cell Biology 2024Quote: ... The click reaction was performed for 2 hrs at room temperature using 2 μM of the Alexa647 alkyne (Thermo Fisher Scientific, A10278), in a copper-mediated click reaction with CuSO4 ...