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Citations for Thermo Fisher :
301 - 350 of 5122 citations for Cytomegalovirus Purified Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... 25 μg F antigen plasmid and 80 μl of ExpiFectamineTM CHO reagent were mixed in 1.9 ml of cold OptiPRO™ medium (Thermo Fisher). After the first feed on day 1 ...
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An mRNA processing pathway suppresses metastasis by governing translational control from the nucleusbioRxiv - Cancer Biology 2021Quote: ... purified using MyOne C1 Dynabeads (Invitrogen), and then circularized using CircLigase II (Epicentre) ...
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bioRxiv - Cell Biology 2020Quote: ... purified by Ni-NTA chromatography (Invitrogen), and used to immunize a guinea pig (Covance ...
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bioRxiv - Genomics 2020Quote: ... Adapter-tagmented fragments were purified (Invitrogen Agencourt AMPure XP beads ...
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bioRxiv - Genetics 2019Quote: ... purified using the MEGAclear Kit (Ambion), ethanol precipitated ...
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bioRxiv - Microbiology 2020Quote: ... and purified by deoxyribonuclease I (Invitrogen) treatment ...
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bioRxiv - Molecular Biology 2020Quote: ... purified by PCR Purification Kit (Invitrogen) and sent to Genewiz for sequencing.
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bioRxiv - Developmental Biology 2019Quote: ... and purified with DNase treatment (Ambion DNA-free DNA Removal Kit ...
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bioRxiv - Neuroscience 2022Quote: ... and purified nuclease free water (Ambion) before being incubated at 37°C for 2 hours ...
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bioRxiv - Genomics 2021Quote: ... RNA was purified using TRIzol (Invitrogen) following manufacturer’s instructions and sent to Genewiz for strand-oriented ...
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bioRxiv - Genetics 2020Quote: ... purified using TRIzol™ (ThermoFisher Scientific) and microinjected into the one-cell stage embryo at doses of 5 ...
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bioRxiv - Neuroscience 2020Quote: ... and purified with Megaclear columns (Ambion). The cassette of interest was introduced into the mouse genome via pronuclear injection of in vitro transcribed TALENs RNA and ROSA donor DNA ...
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Genetic characterization of the zoonotic parasite Ancylostoma caninum in the central and eastern USAbioRxiv - Microbiology 2022Quote: ... Amplicons were enzyme purified (Applied Biosystems, Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... and purified using TURBO DNase (Ambion) followed by the RNeasy Extraction kit (Qiagen) ...
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bioRxiv - Neuroscience 2024Quote: ... and purified using TRIzol (Thermo Fisher), as previously described (84) ...
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bioRxiv - Developmental Biology 2019Quote: ... Immunohistochemical analyses were performed on paraffin sections with traditional antigen retrieval and colorimetric development methodologies with the following primary antibodies: anti-Collagen II (Thermo Scientific, MS235B), anti-Collagen X (Quartett ...
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bioRxiv - Neuroscience 2020Quote: HEK293T cells expressing the SV40 large T antigen (HEK293T, ATCC, USA) were cultured in Dulbecco’s modified Eagle’s medium (DMEM, ThermoFisher Scientific, Scoresby, VIC, Australia), supplemented with 10% heat inactivated foetal bovine serum (FBS ...
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bioRxiv - Microbiology 2020Quote: ... Selection of target-binding nanobodies was performed by phage-display selections against immobilized antigen proteins on Maxisorp plates (NUNC, Thermo Fisher Scientific). The initial screening was performed against CS1and CS2 ...
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bioRxiv - Microbiology 2020Quote: ... Selection of target-binding nanobodies was performed by phage-display selections against immobilized antigen proteins on Maxisorp plates (NUNC, Thermo Fisher Scientific). The initial screening was performed against CS1and CS2 ...
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bioRxiv - Microbiology 2020Quote: ELISA was carried out to estimate the avidity and overall stability of antigen-antibody complexes using the chaotropic agent ammonium thiocyanate (NH4SCN) (Acros Organics Fisher Scientific). The plates were coated with 50 ng of PvRBP2b161-1454 in PBS and incubated overnight ...
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bioRxiv - Immunology 2022Quote: Antigen and adjuvant vaccine components were diluted in DPBS (Cytiva, Marlborough, Massachusetts, USA) and then admixed in glass vials (Thermo Fisher Scientific) prior to administration ...
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bioRxiv - Neuroscience 2021Quote: ... all sections were autoclaved for antigen retrieval and immunohistochemical stained with anti-phosphorylated antibodies AT-8 (pSer202/pThr205, MN1020, Invitrogen, 1:250). Immunolabeling was then examined using a DM4000 microscope (Leica ...
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bioRxiv - Physiology 2019Quote: ... Inclusion bodies of the antigen peptides were harvested from the transformant Escherichia coli BL21 Star (DE3) (Thermo Fisher Scientific, Waltham, MA, USA) (37) ...
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bioRxiv - Immunology 2021Quote: ... Washed yeast libraries were stained in dark and at 4°C with biotinylated antigens pre-conjugated with SA-PE (Streptavidin, R-Phycoerythrin Conjugate, Thermo Fisher Scientific) or SA-APC (Streptavidin ...
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bioRxiv - Immunology 2021Quote: SARS-CoV-2 antigen sequences were cloned into pcDNA3.4 plasmids and transfected into 293F cells utilizing the Expi293 Expression System (Life Technologies, Cat # A14635) per manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... were coated overnight with either Dengue 2 fixed virus antigen (Microbix, Cat# EL-22-02) or polyvalent total immunoglobulin (Invitrogen, Cat# H17000). Before adding PBMC’s ...
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bioRxiv - Immunology 2020Quote: ... the RBD antigen (diluted to 2 μg/ml in PBS) was first adsorbed to 96-well clear Immulon 4 HBX (Thermo Scientific, #3855) plates in PBS overnight at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... A33 and B5 antigens were coupled via carboxyl chemistry to Magplex® fluorescently bar-coded beads (Luminex Corporation) with sulfo-NHS and EDC (Thermo Fisher) per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... and masked epitopes were recovered with eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) (Invitrogen, Waltham, MA, USA, #00-4956-58). Slides were blocked for 1 hour at room temperature with blocking solution (1xPBS ...
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bioRxiv - Immunology 2023Quote: ... Cells were subsequently stained for viability and surface antigens and then intracellularly stained using the eBioscience Intracellular Fixation and Permeabilization Kit (Thermo Fisher Scientific) using the antibodies and reagents listed in Supplemental Table 5.
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bioRxiv - Pathology 2021Quote: ... for 1 week and pepsin (1 mg/ml in 0.1N HCl) was used for antigen retrieval for immunostaining with human COMP (Thermofisher; MA1-20221, 1:100), CHOP (Santa Cruz ...
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bioRxiv - Molecular Biology 2020Quote: ... a female human embryonic kidney cell line transformed with the SV40 large T antigen was obtained from ATCC (#CRL-1573) and cultured in complete media consisting of DMEM (#11965092, GIBCO, Life Technologies; Carlsbad, CA) supplemented with 10% FBS (Seradigm Premium Grade HI FBS ...
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bioRxiv - Microbiology 2020Quote: ELISA was carried out to estimate the avidity and overall stability of antigen-antibody complexes using the chaotropic agent ammonium thiocyanate (NH4SCN) (Acros Organics Fisher Scientific). The plates were coated with 50 ng of PvRBP2b161-1454 in PBS and incubated overnight ...
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bioRxiv - Biophysics 2022Quote: ... We revealed the antigens coating with a Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor 647, Invitrogen, ref. A-21447) and analyzed the fluorescence intensity ...
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bioRxiv - Microbiology 2020Quote: Total RNA was extracted from cell pellets collected in QuantiFERON-TB gold assay tubes without antigen using the Ribopure Ambion RNA isolation kit (Life Technologies, California, USA), and eluted RNA treated with DNase for 30 min ...
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bioRxiv - Neuroscience 2021Quote: ... and adjusted tissue and plasma extracts were added with the antiserum against OT (1:1,000 dilution) to each antigen-coated well of a 96-well microplate (F96 maxisorp nunc-immuno plate;Thermo Fisher Scientific, Roskilde, Denmark) and incubated overnight at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... for antigen retrieval in a pressure cooker for 10 minutes then sections were blocked in CAS-block histochemical reagent (Thermo Fisher Scientific, 008120). Permeabilization was performed with 0.5% Triton X-100 in PBS for 5 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... Both FFPE and OCT cell array samples were incubated for 5min at 100°C in 1x Antigen Retrieval Solution (ThermoFisher, 00-4956-58) using a pressure cooker ...
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bioRxiv - Pathology 2023Quote: Slides were incubated at 90°C for 20 minutes in High pH IHC Antigen Retrieval Solution (Thermo Fisher Scientific Catalog Number 00-4956) and allowed to cool to room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... by measuring the expression of pluripotency surface antigen (SSEA4 1:20, Invitrogen, 12-8843-42, and TRA1-60 1:20, Invitrogen, 12-8863-82) by flow cytometry ...
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bioRxiv - Genetics 2021Quote: RNAseq: 5 μg of total Trizol-purified RNA purified was treated with Turbo DNase free kit (Thermo Fisher), ribosomal depleted using Ribo-Zero Kit (Epicentre ...
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bioRxiv - Plant Biology 2020Quote: ... PCR products were purified using PureLinkTm (Invitrogen) and sequenced by Sanger without cloning.
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bioRxiv - Genetics 2021Quote: ... mRNA was purified using MegaClear kit (Ambion) and eluted in 100 μL of RNase-free water ...
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR products were purified with ExoSAP (Affymetrix) and sequenced in both directions using an ABI 3500 Genetic Analyzer (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2022Quote: Total RNA was purified using TRIZOL (Ambion) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... and purified using Megaclear Kit (Life Technologies). sgRNAs were tested in vitro for their cleavage ability in combination with Cas9 nuclease (New England Biolabs) ...
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bioRxiv - Developmental Biology 2020Quote: ... Purified RNA was treated with DNase (Ambion) for 1hr at 37°C ...
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bioRxiv - Molecular Biology 2019Quote: ... and further purified with TRIzol (Thermo Fisher). Poly-A RNA was isolated using Dynabead oligo dT25 magnetic beads (Thermo Fisher ...
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bioRxiv - Neuroscience 2020Quote: ... Purified RNAs were quantified with Ribogreen (Invitrogen) and 10-50 ng of RNA were used for reverse transcription with SensiFAST cDNA synthesis kit (Bioline ...
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bioRxiv - Bioengineering 2021Quote: Total RNA was purified using TRIzol (Invitrogen) and reverse-transcribed using AccuPower RT PreMix (Bioneer ...