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Citations for Thermo Fisher :
401 - 450 of 10000+ citations for Cytomegalovirus Cell Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... The following antigens were coupled with carboxylated beads (Luminex Corp): BSA (Affymetrix), goat anti-mouse Igκ ...
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bioRxiv - Immunology 2023Quote: ... Biotinylated antigen was then added to FITC-conjugated neutravidin beads (ThermoFisher, F8776) at an antigen(μg):bead(μL ...
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bioRxiv - Cancer Biology 2023Quote: ... After treating them with IHC Antigen Retrieval Solution (00-4956-58, Invitrogen), the sections were stained with an anti-human CD4 Ab that was pre-labeled with Alexa Fluor 488 using an Ab labeling kit (Molecular Probes ...
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bioRxiv - Physiology 2023Quote: ... and targeted antigen retrieval performed using boiling 1x Targeted Retrieval Solution (Invitrogen) for 20 mins ...
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bioRxiv - Immunology 2021Quote: SARS-CoV-2 antigen sequences were cloned into pcDNA3.4 plasmids and transfected into 293F cells utilizing the Expi293 Expression System (Life Technologies, Cat # A14635) per manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Whole cell lysates (WCL) were prepared by resuspending cell pellets in RIPA lysis and extraction buffer (Thermo Fisher Scientific, #89901) containing a Protease/Phosphatase inhibitor cocktail (Cell Signaling Technology (CST) ...
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bioRxiv - Immunology 2019Quote: ... cells were lysed and lysates were analyzed by qRT-PCR with a Cells-to-CT Power SYBR Green kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... cell lysates prepared from knockdown and control cells were tested by western blotting with antibodies directed to CTSL (Invitrogen, BMS1032), CCZ1 (Santa Cruz Biotechnology ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA were extracted from the cell lysates of both single-cultured and co-cultured MCF7 cells using Trizol reagent (Invitrogen). RNA quality was assessed by Agilent 2100 bioanalyzer (Agilent Technologies ...
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bioRxiv - Immunology 2021Quote: Cell lysates were obtained by diluting cell pellets in NuPAGE™ LDS Sample Buffer (4X) (Thermo Fisher Scientific, Cat# NP0007) and incubating at 99°C for 5 minutes, ...
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Differential expression of transposable elements in stem cell lineages of the preimplantation embryobioRxiv - Developmental Biology 2020Quote: Total RNA was extracted from the lysates of two wildtype ES cell replicates and five wildtype TS cell replicates with TRIzol (Invitrogen). 500 ng of total RNA was used for each RNA-seq library preparation ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was synthesized in cell lysates and amplified using the TaqMan® PreAmp Cells-to-Ct KitTM (Applied Biosystems; #4387299) and TaqMan® Gene Expression Assays (Applied Biosystems ...
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bioRxiv - Microbiology 2024Quote: ... Whole cell lysates were made by lysing cells with RIPA buffer containing Halt protease and phosphatase inhibitor cocktail (ThermoFisher, 78440) and Dnase I (ThermoFisher ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were treated with 0.5 U DNase I per 100 uL lysate (Invitrogen, Waltham, MA, USA) for 15 min ...
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bioRxiv - Microbiology 2020Quote: Viral RNA from SARS-CoV-2 infected HBEC cell lysates was extracted using TRIzol (Life Technologies) and purified using Direct-zol RNA MiniPrep Plus according to manufacturer’s instructions (Zymo Research ...
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bioRxiv - Microbiology 2021Quote: ... The clarified cell lysate was mixed with 10 units of RiboLock RNase Inhibitor (Thermo Scientific, USA) and then incubated with primary antibody or an equivalent volume of IP buffer (beads control ...
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Polo-like kinase 1 independently controls microtubule-nucleating capacity and size of the centrosomebioRxiv - Cell Biology 2020Quote: ... the whole cell lysates were incubated with Pierce Anti-c-Myc magnetic beads (Thermo Fisher Scientific) for 2 hours at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... The cell lysate was then dialysed using Slide-A-Lyzer dialysis cassette (3500 MWCO, ThermoFisher Scientific) to remove unreacted free BP ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell lysates were resolved under denaturing conditions by SDS-PAGE (4–12% Bis-Tris gels; Invitrogen) and transferred to nitrocellulose membrane using Trans-Blot Turbo Transfer System (Bio-rad) ...
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bioRxiv - Molecular Biology 2020Quote: ... lysates (~5 × 106 cells per experiment) were pre-cleared with PierceTM Protein A Agarose (Thermo Scientific) for 2 hr at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Cell lysates were prepared in the modified RIPA buffer containing protease inhibitors (Thermo Scientific, Rockford, IL). Equal amounts of proteins ...
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bioRxiv - Immunology 2019Quote: ... Protein concentrations of the cell lysates were quantified using a BCA protein assay (Thermo Fisher Scientific) to ensure equal amounts of total protein were loaded in each well of NuPAGE 4–12% Bis-Tris Gel (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell lysates (15-20 ug of protein) were resolved in 4%-12% Bis-Tris gels (Invitrogen) and transferred to PVDF membranes using wet transfer ...
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bioRxiv - Biochemistry 2019Quote: ... RNA extracted from the total cell lysate using the GeneJET RNA purification kit (Thermo Fisher Scientific) was used to IP to serve as a reference sample ...
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bioRxiv - Genomics 2020Quote: Poly-adenylated mRNA was isolated from thawed cell lysates using the Dynabeads mRNA Direct Kit (Ambion) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... The cell lysate was sonicated (duty cycle 20%, output control 4, Sonic Dismembrator 550, Fisher Scientific) for 10 minutes on ice ...
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bioRxiv - Biochemistry 2020Quote: ... non-denatured cell lysates were separated on NativePAGE™ 3-12% Bis-Tris gel (Thermo Fisher) following manufacturer’s procedure ...
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bioRxiv - Neuroscience 2020Quote: ... The cell lysate was then quantified for protein using a BCA Assay Kit (Thermo Fisher Scientific) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Lysates from FIRE-pHLy-expressing HEK293FT cells were collected from 1X RIPA buffer (Fisher Scientific, #89900) supplemented with a cocktail of phosphatase and protease inhibitors (Roche ...
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bioRxiv - Systems Biology 2021Quote: ... Cell lysates were mixed with BOLT LDS sample buffer supplemented with a reducing agent (Life Technologies) and boiled for 5 minutes ...
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bioRxiv - Microbiology 2019Quote: Cell lysates and immunoprecipitates were separated on NuPage Novex 4-12% Bis-Tris Mini Gels (Invitrogen), and NuPAGE™ MES SDS running buffer (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein concentrations from whole cell lysates were determined using a Bradford reagent (Thermo Fisher, Carlsbad, CA). Protein samples (50µg total protein ...
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bioRxiv - Cell Biology 2021Quote: ... Total protein concentration in cell lysates was determined using Qubit™ Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Cell lysates were prepared using a RIPA buffer and incubated with Streptavidin-agarose beads (Thermo Scientific) using end-over-end agitation at 4°C overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein concentration of cleared cell lysates was determined by BCA protein assay kit (Thermo Scientific #23225) and 20-30 μg of lysed samples were subjected to SDS-polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Molecular Biology 2022Quote: ... cytosolic RNAs were isolated from cell lysates using the subcellular protein fractionation kit (Thermo Fisher Scientific) following the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2022Quote: ERα proteins were immunoprecipitated from J774a.1 cell lysates according to the manufacturer’s protocols (11204D, Invitrogen). The ERα antibody was the same as that used for Western blotting and was purchased from Santa Cruz (1:200 dilution) ...
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bioRxiv - Cell Biology 2022Quote: RNA was isolated from virus-containing medium or cell lysates using TRIzol LS (ThermoFisher Scientific, #10296010) according to the manufacturer’s instructions and stored at −80°C for RT-qPCR ...
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bioRxiv - Molecular Biology 2020Quote: Cell lysate total protein was determined by using the Pierce Bicinchoninic acid (BCA) assay (Thermo Scientific), following the instructions of the manufacturer ...
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bioRxiv - Molecular Biology 2022Quote: ... the cell lysates were incubated with 2 μg anti-V5 antibody per dish (Invitrogen, Cat # R960CUS) for 3 h at 4°C on a rotating wheel ...
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: Cell lysates and immunoprecipitates were separated on NuPage Novex 4-12% Bis-Tris Mini Gels (Invitrogen), and NuPAGE™ MES SDS running buffer (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: Clarified cell lysates and TCA precipitated media were resuspended in LDS sample buffer (Thermo Life Technologies), resolved on 4-12 % NuPAGE gels (Thermo Life Technologies ...
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bioRxiv - Molecular Biology 2019Quote: Whole cell lysates were resolved in NuPAGETM 3-8% or 4-12% gradient gels (ThermoFisher Scientific) and transferred onto PVDF membranes using the Trans-blot Turbo transfer system (BioRad) ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were harvested and lysates prepared using M-PER mammalian protein extraction reagent (Thermo Fisher Scientific), and protein concentration was measured using a bicinchoninic acid (BCA ...
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HIV-1 Nef interacts with LMP7 to attenuate immunoproteasome formation and MHC-I antigen presentationbioRxiv - Microbiology 2019Quote: ... cell lysate supernatants were mixed with antibodies and recombinant protein G-Sepharose 4B beads (Thermo Fisher) at 4°C for overnight ...
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bioRxiv - Cell Biology 2021Quote: ... HRP in the cell lysate was detected by the addition of 50 μM Amplex Red (Invitrogen, in 150 mM NaCl ...
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bioRxiv - Cell Biology 2021Quote: Cell lysates were processed with NE-PER Nuclear and Cytoplasmic Extraction Kit (78835, Thermo Fisher Scientific) to separate the cytoplasmic and nuclear fractions ...
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bioRxiv - Neuroscience 2020Quote: ... Gene expression was measured in cell lysates using a chemoluminescent substrate of galactosidase (GalactoStar kit, Invitrogen). Neutralizing factor titer corresponds to the last dilution leading to the inhibition of more than 50% of the standard AAV2/9 transduction (100% corresponds to the maximum transduction obtained with the standard AAV2/9 alone) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cell lysate supernatant was applied to a pre-packed and conditioned HisPur Ni-NTA column (ThermoFisher) overnight at a low flow rate (0.5 mL/min) ...
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bioRxiv - Biophysics 2019Quote: U2OS cell lysates were collected in Pierce RIPA buffer (Cat#89900; Lot no. NF170965; ThermoFisher Scientific) supplemented with Complete protease inhibitors (Roche ...