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Citations for Thermo Fisher :
651 - 700 of 10000+ citations for Crimean Congo Hemorrhagic Fever Virus Gn Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Protein concentration was determined using EZQ protein quantitation kit (Invitrogen) as per manufacturer instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentration was determined using EZQ protein quantitation kit (Invitrogen) as per the manufacturer instructions ...
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bioRxiv - Physiology 2022Quote: ... and protein quantified with a BCA protein assay (Thermo Scientific). Protein (40 µg ...
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bioRxiv - Immunology 2022Quote: ... Protein concentration was determined by BCA Protein Assay kit (ThermoFisher). 10μg/sample with 4x Laemmli Buffer (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... protein concentrations were determined using the BCA protein assay (ThermoFisher) and assessed with a FLUOstar Omega microplate reader (BMG Labtech) ...
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An adaptive biomolecular condensation response is conserved across environmentally divergent speciesbioRxiv - Cell Biology 2023Quote: ... Protein quantitation was performed using a Qubit protein assay (Invitrogen), protein yields are provided below ...
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bioRxiv - Biochemistry 2023Quote: ... a known protein size marker (NativeMarkTM Unstained Protein Standard, Invitrogen) was measured.
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bioRxiv - Developmental Biology 2023Quote: ... 20 µL protein A beads (Dynabeads Protein A, ThermoFisher Scientific) blocked with BSA were added and samples were incubated at 4°C for 4 hours ...
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bioRxiv - Cell Biology 2023Quote: ... Protein determinations were performed using bicinchoninic acid protein assay (ThermoFisher).
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bioRxiv - Neuroscience 2023Quote: ... Protein-A and Protein-G Dynabeads (Invitrogen, #10002D and #10004D) were added to each tube and incubated for an additional 20-30 min with gentle rotation at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... AGO protein immunoprecipitation was performed using Protein G Dynabeads (Invitrogen), and 10μl 2A8 anti-Ago antibody (Millipore ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein content was measured using bicinchoninic acid protein assay (ThermoFisher). For evaluation of systemic cytokine signaling ...
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bioRxiv - Microbiology 2023Quote: ... Protein concentrations were determined using Qubit Protein Assay Kit (Invitrogen). We used 30 μg protein ...
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bioRxiv - Cell Biology 2023Quote: ... Equal volumes of protein A- and protein G-Dynabeads (Invitrogen) were mixed and prepared by washing in ChIP dilution buffer (16.7 mM Tris-HCl pH 8 ...
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bioRxiv - Microbiology 2023Quote: ... Protein concentrations were quantified using EZQ protein assay kit (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Immunoprecipitated proteins were collected on Protein-G Dynabeads (Invitrogen #10004D), separated by SDS-PAGE (Mini PROTEAN TGX ...
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bioRxiv - Neuroscience 2024Quote: ... Protein was quantified using the BCA protein assay (ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: 10 µl of Protein A beads (DynabeadsTM Protein A; Invitrogen) equivalent to 10 µl bed volume of slurry were prepared per each ChIP reaction ...
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bioRxiv - Immunology 2023Quote: ... protein was quantified by Pierce BCA Protein Assay (ThermoFisher #23225), XT sample buffer + reducing agent ...
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bioRxiv - Immunology 2023Quote: ... Protein concentration was determined using EZQ protein quantitation kit (Invitrogen) as per manufacturer instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Protein concentrations were quantified by BCA protein assays (ThermoFisher Scientific).
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bioRxiv - Developmental Biology 2024Quote: ... After protein quantification with BCA protein assay kit (23225, ThermoFisher), samples were mixed with SDS gel loading buffer (L-4390 ...
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bioRxiv - Cancer Biology 2024Quote: ... and protein quantified by Pierce BCA Protein Assay (Thermo Scientific). Protein samples were diluted to equal concentrations in lysis buffer and boiled for 5 minutes in 1X Laemmli Sample Buffer ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentrations were determined using Pierce BCA protein assay (ThermoFisher). 10 to 30 μg protein was loaded on 4-12% Bis-Tris Plus precast gels (ThermoFisher ...
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bioRxiv - Cell Biology 2024Quote: ... To estimate protein sizes prestained protein ladder from ThermoFisher (#26616) was used ...
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bioRxiv - Molecular Biology 2023Quote: ... Ubiquitinated proteins were immunoprecipitated using magnetic Protein A Dynabeads (Thermofisher) loaded with an antibody against His-tagged proteins (Finetest ...
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bioRxiv - Biophysics 2024Quote: ... Proteins were purified with FLAG-functionalized Dynabeads Protein G (Invitrogen), dye-labeled on beads with tetrazine functionalized-Cy3 and ATTO647N (Jena Bioscience ...
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bioRxiv - Cell Biology 2020Quote: ... HeLa cells were induced to produce mov10-YFP by supplementation of the medium with 1 µg/ml doxycycline and then infected with sindbis virus (SINV-mCherry, see below [33]) and then incubated with MitoTracker Deep Red (Thermo Fisher) at 125 nM for 30 minutes before being plunge frozen as above for imaging.
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bioRxiv - Molecular Biology 2021Quote: ... and puromycin resistance marker-Bovine Growth Harmone polyA-signal (amplified from pMK232 (Natsume et al., 2016)) or blasticidin resistance marker-Simian Virus 40 polyA-signal (amplified from pcDNA6/TR (Thermo Fisher)) were cloned into pTwist-Amp (Twist Bioscience ...
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bioRxiv - Molecular Biology 2021Quote: ... was performed using double nucleic acid detection kit for respiratory syncytial virus (A/B) (Shenzhen shengkeyuan) with the ABI Q7 (Applied Biosystems). The reaction was conducted according to the manufacturer’s instructions with total volume of 20 μL.
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bioRxiv - Immunology 2022Quote: ... Two micrograms of liver RNA per sample was used for qRT-PCR analysis for Plasmodium 18S rRNA using TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems). Data were normalized for input to the GAPDH control (hepatocytes ...
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bioRxiv - Immunology 2021Quote: ... The isolated RNA was then evaluated in RT-qPCR using the TaqMan Fast Virus 1-step Master Mix (Thermo Fisher Scientific) on a QuantStudio Flex 6 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Bioengineering 2019Quote: iPSCs were reprogrammed from peripheral blood mononuclear cells using the Sendai virus approach (CytoTune-iPS 2.0 Sendai Reprogramming Kit - Thermo Fisher Scientific) according to the manufacturer instruction ...
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bioRxiv - Microbiology 2021Quote: ... and infected at a MOI of 1 with the VSV-ΔG-Fluc-2A-GFP/VSV-G virus diluted in 10mL Opti-MEM (Life Technologies). The cells were incubated 1 hour at 37C with 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: ZIKV RNA samples were each measured in triplicate on an Applied Biosystems ViiA 7 machine using a Taqman Fast Virus 1-Step MasterMix (Thermo Fisher) with primers ZIKV 1087 forward (CCGCTGCCCAACACAAG) ...
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bioRxiv - Microbiology 2020Quote: ... 24 h later the dish was washed with PBS and treated with PR8 virus (MOI =2) in the infection medium [F-12K with 2% BSA fraction V (Thermo Fisher), 1% antibiotic-antimycotic (Thermo Fisher) ...
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bioRxiv - Microbiology 2020Quote: ... 1.5 ml microcentrifuge polypropylene tubes containing the virus (500 µL total volume) were exposed to direct heat in a heat block (Fisher Scientific). After heating ...
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bioRxiv - Immunology 2021Quote: ... The mouse organ tissues were homogenized to detect the virus gene copy number by qPCR (TaqMan Universal Master Mix II with UNG, 4440044, Applied Biosystems). The sequences of the primers are listed in Table 2.
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bioRxiv - Microbiology 2021Quote: ... were seeded in 96-well plates 48h prior to virus inoculation at a density of 5×104 in DMEM (Gibco #41965039) supplemented as described above ...
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bioRxiv - Cancer Biology 2021Quote: ... or empty pLKO.1-puro lentiviral vector were transfected together with pCMV-dR8.9 and vesicular stomatitis virus G–expressing plasmids into 293T cells using Lipofectamine 2000 (Thermo Fisher Scientific) for lentiviral supernatant production as previously described (48) ...
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bioRxiv - Neuroscience 2022Quote: LC-MS/MS analyses were conducted using either a QExactive Plus Orbitrap (QE, RNase-digested polysomes) or a Velos Pro Elite Orbitrap (Elite, virus polysomes and HEK293 aggregates) mass spectrometer (Thermo Fisher) coupled online to a nanoAcquity UPLC system (Waters Corporation ...
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bioRxiv - Cell Biology 2022Quote: ... the unbound virus was removed by washing with infection media followed by agarose overlay with 1X MEM Temin’s modification (Thermo Fisher, 11935046) +0.3% agarose ...
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bioRxiv - Microbiology 2022Quote: Viral RNA in apical secretions was quantified using the TaqMan™ Fast-Virus-1 Step master mix (Thermo Fisher, Basel, Switzerland) with these cycling parameters ...
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bioRxiv - Microbiology 2022Quote: ... HuNoV RNA genome equivalents (GEs) were determined by reverse transcription-quantitative PCR (RT-qPCR) analysis using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher) and GII specific primer/probe sets (Kageyama et al. ...
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bioRxiv - Bioengineering 2022Quote: ... infected cells were counted and determined by evaluating GFP expression after 24 hours exposure to virus-antibody mixture using Attune NxT Acoustic Focusing Cytometer (Thermo Fisher). Half-maximal inhibitory concentration (IC50 ...
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bioRxiv - Plant Biology 2021Quote: ... and then incorporated into the destination vector pMV3 (driven by the cauliflower mosaic virus (CaMV) 35S promoter) using the LR recombination reaction (Invitrogen, USA). All the recombinant constructs were transformed into the Agrobacterium tumefaciens strain C58 by electroporation and subsequently transformed into the tomato genome via explants of cotyledon ...
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bioRxiv - Developmental Biology 2021Quote: ... The virus was spun at 500 g for 5 min and the supernatant filtered through a 45 μm polyethersulfone filter (Fisher Scientific) and subsequently concentrated ...
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bioRxiv - Microbiology 2021Quote: ... viral stocks were prepared after two serial passages of isolated virus in Vero E6 cells in Dulbecco’s Modified Eagle Medium (DMEM) (Thermo Fisher Scientific) supplemented with 5% fetal bovine serum (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... Sf9 transfection with bacmid DNA and virus amplification were performed essentially as described for the Bac-to-Bac Baculovirus Expression System (ThermoFisher Scientific). Sf9 cells were maintained in Hyclone CCM3 (CCM3 liquid medium with L-glutamine ...
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bioRxiv - Immunology 2019Quote: ... and cDNA was reverse transcribed using the Moloney murine leukemia virus reverse transcriptase and random hexamer oligonucleotides for priming (Life Technologies). The expression of CPT1 was determined using PCR SYBR Green sequence detection system (Eurogentec ...