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Citations for Thermo Fisher :
251 - 300 of 10000+ citations for Cow T Cell Surface Glycoprotein CD3 Gamma Chain CD3G ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: Cell culture supernatants collected at various time intervals from in vitro stimulated T cells of mice or humans were analyzed for IL-2 by ELISA using kits purchased from ThermoFisher Scientific (mouse IL-2 ELISA kit ...
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bioRxiv - Immunology 2021Quote: ... Cells were lysed immediately or stimulated at a ratio of 1:1 with Dynabeads™ Mouse T-Activator CD3/CD28 for T-Cell Expansion and Activation (Gibco, 1152D) for 16 hours (qPCR and RNAseq) ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated for 2 hours prior to stimulation with ratio of 1:1 with Dynabeads™ Mouse T-Activator CD3/CD28 for T-Cell Expansion and Activation (Gibco, 1152D) for 48 hours ...
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bioRxiv - Immunology 2021Quote: ... and 1:1 as shown in Table 2 and stimulated at a ratio of 1:1 with Dynabeads™ Mouse T-Activator CD3/CD28 for T-Cell Expansion and Activation (Gibco, 1152D). After 5 days ...
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bioRxiv - Immunology 2022Quote: Single cell suspensions of human PBMC were stimulated with Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation (ThermoFisher Scientific) as per manufacturer’s instructions.
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bioRxiv - Immunology 2019Quote: ... 1×106 (24-well plate) or 5×105 (48-well plate) CD8+ T-cells were activated with Dynabeads Mouse T-Activator CD3/CD28 (Thermo Fisher, 11456D) in a 1:1 beat to T-cell ratio and cultured in 2 ml (24WP ...
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bioRxiv - Immunology 2021Quote: ... 1×106 (24-well plate) or 5×105 (48-well plate) CD8+ T cells were activated with Dynabeads Mouse T-Activator CD3/CD28 (Thermo Fisher, 11456D) in a 1:1 bead to T cell ratio and cultured in 2 ml (24-well plate ...
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bioRxiv - Molecular Biology 2020Quote: ... Stimulation of T-cell proliferation was achieved by incubation with CD3/CD28 antibody beads (Thermo Fisher). Carboxyfluorescein succinimidyl ester (CFSE ...
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bioRxiv - Immunology 2021Quote: ... Naïve CD8+ cells were plated with Human T-Activator CD3/CD28 Dynabeads (Thermofisher, Cat no.111.31D) at 25 µl of Dynabeads per 1x106 CD8+ cells in complete ImmunoCult cell culture media (see above) ...
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bioRxiv - Immunology 2022Quote: ... cells were pre-activated with Dynabeads human T-Activator CD3/CD28 (Thermo Fisher Scientific, cat#111.31D) for 24 h and beads were removed before electroporation ...
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bioRxiv - Immunology 2019Quote: ... Isolated Vα7.2+ cells were activated with Dynabeads™ human T-activator CD3/CD28 (Gibco, ThermoFisher Scientific) at a ratio of 1:1 ...
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bioRxiv - Immunology 2019Quote: ... Isolated Vα7.2+ cells were activated with Dynabeads™ human T-activator CD3/CD28 (Gibco, ThermoFisher Scientific) at a ratio of 1:1 ...
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bioRxiv - Immunology 2020Quote: ... The nTreg cells were cultured in the presence of Dynabeads Mouse T-Activator CD3/CD28 (Invitrogen) with 500 U/mL recombinant human IL-2 for 4 days and then given fresh media with recombinant human IL-2 and indicated cytokines or compounds for 4 more days ...
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bioRxiv - Immunology 2020Quote: ... CD3+ T cells were labeled with the CellTrace carboxyfluorescein diacetate succinimide ester (CFSE; ThermoFisher, Cat. C34554), and then cultured with 30 U/mL rIL-2 (#34-8021-85 ...
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bioRxiv - Cancer Biology 2022Quote: ... Isolated T cells were cultured with anti-CD3/CD28 beads (Dynabeads Cat. # 11452D; Thermo Fisher Scientific) in a 1:1 ratio in 10% complete RPMI (RPMI 1640 medium supplemented with 10% FBS ...
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bioRxiv - Immunology 2022Quote: ... All human CD8+ T cells were activated with anti-human CD3/CD28 dynabeads (11131D, Thermo Scientific) at a 1:1 cell-to-bead ratio and cultured in complete RPMI supplemented with 10% FBS ...
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bioRxiv - Immunology 2022Quote: ... WT mouse CD8+ T cells were activated with anti-mouse CD3/CD28 dynabeads (11453D, Thermo Scientific) at a 1:1 cell-to-bead ratio ...
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bioRxiv - Bioengineering 2023Quote: ... Prior to electroporation isolated human T cells were activated for 48h with CD3/CD28 Dynabeads (Thermofisher) at a cell to beads ratio 1:1 together with the recombinant human cytokines IL-2 (150U/ml) ...
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bioRxiv - Bioengineering 2023Quote: ... Isolated CD8+ or CD4+ T cells were stimulated with anti-CD3/CD28 beads (Thermo Fisher Scientific) at a 2:1 bead ...
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bioRxiv - Biophysics 2022Quote: Oocyte surface proteins were purified using the Pierce Cell Surface Protein Isolation Kit (Thermo Fisher Scientific), modified here for oocytes as previously described (Lynagh ...
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bioRxiv - Synthetic Biology 2020Quote: ... Surface proteins were purified with the Pierce™ Cell Surface Protein Isolation Kit (Thermo Fisher Scientific). Purified surface proteins or total cell lysates were run on a 4–12 % BIS-TRIS gel (for P2X2 ...
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bioRxiv - Neuroscience 2020Quote: The cell surface proteins of hippocampus were isolated using Pierce cell surface protein isolation kit (Pierce, Thermo Scientific, MA). Biotinylated proteins bound to NeutrAvidin beads (bound fraction ...
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bioRxiv - Physiology 2023Quote: Cell surface proteins from transfected HEK293 cells were biotinylated using the Pierce Cell Surface Protein Isolation Kit (ThermoFisher Scientific., cat# 89881). Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... a cell surface biotinylation assay was performed using a commercially available kit (Pierce Cell Surface Protein Isolation Kit, Thermo Fisher Scientific, catalog #89881). Eluates were subjected to immunoblot analysis using a Plexin-A4 antibody (Cell Signaling Technology ...
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bioRxiv - Systems Biology 2020Quote: ... and a second part was stained and analysed for glycoproteins using the Pro-Q 488 Emerald Glycoprotein staining kit (P33375, Thermo/Invitrogen), according to the manufacturers protocol ...
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bioRxiv - Immunology 2020Quote: ... Pro-Q™ Emerald 300 glycoprotein gel stain kit (Invitrogen) was used to detect the presence of sugar in Bl-Eng2 before and after deglycosylation ...
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bioRxiv - Immunology 2023Quote: Cryopreserved human PBMCs were thawed and activated the same day with Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation (Gibco, Cat#11131D) at 1:1 beads:cell ratio in T cell medium containing 150 IU/mL of IL-2 ...
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bioRxiv - Genomics 2019Quote: ... 500 μL cRPMI with human T-Activator CD3/CD28 Dynabeads (Gibco) were added to each well at a ratio of 2 cells ...
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bioRxiv - Immunology 2022Quote: ... and activated with Human T-Activator CD3/CD28 Dynabeads™ (ThermoFisher). On Day 1 cells were concentrated to 4 × 106/ml then either transduced with lentiviral vectors encoding CAR constructs at an MOI of 2 ...
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bioRxiv - Immunology 2021Quote: ... stimulated with Dynabeads™ Human T-Activator CD3/CD28 (ThermoFisher Scientific) with 17 ng/ml of recombinant human IL-2 (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... and activated with Dynabeads Human T Activator CD3/CD28 (Gibco, USA) at a 1:1 ratio for 24 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... stimulated with CD3/CD28 T-Activator and mouse IL-2 (Gibco), and cocultured with F4/80+ macrophages at 2:1 (T cells:macrophages ...
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bioRxiv - Cancer Biology 2021Quote: ... TCR-transduced T cells were expanded by stimulation with anti-CD3/CD28 Dynabeads (500,000 beads/106 cells; Life Technologies) and IL-2 (50 IU/mL) ...
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bioRxiv - Immunology 2021Quote: ... TCR-transduced T cells were expanded by stimulation with anti-CD3/CD28 Dynabeads (500,000 beads/106 cells; Life Technologies) and IL-2 (50 IU/mL) ...
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bioRxiv - Cell Biology 2022Quote: Surface proteins on Caco-2 cells were biotinylated using Pierce Cell Surface protein biotinylation and Isolation Kit (Thermo Scientific, A44390) following the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: EV surface protein enrichment was performed by Pierce cell surface protein biotinylation and isolation kit (Thermo Scientific) as previous study37 with a modification on washing procedure (replacing ultracentrifugation by ultrafiltration) ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed again and were stained with surface markers BUV395 anti-CD3 (1:167 dilution, clone 17A2, ThermoFisher Scientific), BV785 anti-CD4 (1:100 dilution ...
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bioRxiv - Genetics 2023Quote: Pierce Cell Surface Protein Biotinylation and Isolation Kit (Thermo Scientific) was used to biotinylate and isolate cell surface proteins ...
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bioRxiv - Molecular Biology 2020Quote: ... Prior to electroporation the Primary T cells were stimulated with magnetic anti-CD3/anti-CD28 Dynabeads (ThermoFisher) for 48 hours ...
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bioRxiv - Bioengineering 2022Quote: ... Primary lymphocytes were stimulated with CD3/CD28 T-cell activation Dynabeads (Gibco, Grand Island, NY, United States) for 48 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... Prior to electroporation the Primary T cells were stimulated with magnetic anti-CD3/anti-CD28 Dynabeads (ThermoFisher) for 48 hours ...
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bioRxiv - Immunology 2020Quote: ... Fresh primary human T cells were isolated and cultured with anti-human CD3/CD28 beads (Dynabeads, ThermoFisher), 500U/mL IL-2 (Proleukin ...
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bioRxiv - Immunology 2021Quote: ... Purified (91.0 ± 2.5% purity) naïve CD8+ T cells were activated with α-CD3/CD28 Dynabeads (Life Technologies) in the presence or absence of Apocynin (200µM) ...
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bioRxiv - Immunology 2022Quote: ... Tumor infiltrating CD45+ cells were cultured for 24 hours with dynabeads mouse T-Activator CD3/CD28 (Gibco) for T cell activation ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were activated with 30 U/mL rIL-2 and Dynabeads Human T-activator CD3/CD28 (ThermoFisher) and cultured with the CM of CA-MSCs in the presence or not of CXCR4 Ab (R&D Systems) ...
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bioRxiv - Genomics 2023Quote: ... KRAB-dCas9 expressing cells were activated with Dynabeads Human T-Activator CD3/CD28 (Thermo Fisher Scientific, 11131D), at 1:1 cell:bead ratio ...
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bioRxiv - Physiology 2023Quote: CD4+ T cells were harvested after 72 h of anti-CD3 activation on cytoslides (Thermo scientific, 5991056) using a Shandon cytospin centrifuge (400 rpm for 4 min at RT) ...
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bioRxiv - Bioengineering 2023Quote: ... 1×106 T cells per well were stimulated for 24h with Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation (Thermo Fisher Scientific, 11161D) according to manufacturer’s instructions in a 24-well plate containing X-VIVO 15 media (Lonza ...
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bioRxiv - Immunology 2021Quote: ... CD4+ T cells are enriched using MagniSort Mouse CD4+ T cell Enrichment Kit (Thermofisher scientific) and seeded at a density of 1,5.106 cells/ml with ½ fresh medium and ½ culture medium supplemented with IL-2 (10ng/ml) ...
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bioRxiv - Immunology 2021Quote: ... Enriched CD3+ T cells were cell trace stained and cultured at 1x106 cells/mL and stimulated with mouse T-activator anti-CD3/CD28 Dynabeads (ThermoFisher) at a 1:1 ratio ...