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Citations for Thermo Fisher :
1 - 50 of 4781 citations for Cone Arrestin Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... rod arrestin (1:250, Invitrogen), Stra6 (1:250 ...
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bioRxiv - Biophysics 2024Quote: For labeling β-arrestin 1 with MB (ThermoFisher), the latter was added from a 100 mM stock solution in DMSO to purified arrestin at a 1:10 molar ratio and incubated overnight at 4°C in the dark ...
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bioRxiv - Immunology 2021Quote: Sorted HSC were also cultured with blocking CD150 peptide (FCKQLKLYEQVSPPE, Auspep, 100 μg/ml) or control peptide (DLSKGSYPDHLEDGY, Auspep,100 μg/ml) (Thermo Scientific).
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bioRxiv - Biophysics 2023Quote: ... Purified arrestin was first incubated with 0.8 equivalents of AlexaFluor350 maleimide (ThermoFisher) for 3 hours in the dark ...
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bioRxiv - Cell Biology 2022Quote: ... β-arrestin was labeled by incubation with 0.8 μM Alexa Fluor™ 647 C2 Maleimide (Invitrogen, cat. no. A20347) for 2 h at room temperature in the dark ...
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bioRxiv - Developmental Biology 2021Quote: ... After blocking with blocking buffers (Thermo Scientific) for 30 minutes ...
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bioRxiv - Bioengineering 2023Quote: Murine cone 661W cell line (ATCC, Virginia, USA) was cultivated in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco) complemented with 10% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... Human β-arrestin 2 (residues 1-393, with I386A, V387A, and F388A mutations) was cloned into pBiT2.1 vector (Invitrogen) with the SmBiT at its N-terminus ...
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bioRxiv - Evolutionary Biology 2019Quote: ... the seven cone opsins present in East African cichlids were measured individually using custom Taqman® assays (Invitrogen) with the exception of RH2Aα and RH2Aβ ...
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bioRxiv - Molecular Biology 2021Quote: ... the cells were transiently transfected with HA-Flag-GHRHR-Rluc8 or HA-Flag-SV1-Rluc8 and β-arrestin 1/2-Venus at a 1:9 mass ratio using Lipofectamine 3000 reagent (Invitrogen) and cultured for another 24 h ...
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bioRxiv - Molecular Biology 2022Quote: ... the cells were transiently transfected with HA-GLP-1R-Rluc8 and β-arrestin 1/2-Venus at a 1:9 mass ratio using lipofectamine 3000 reagent (Invitrogen) and cultured for another 24 h ...
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Development of a genetically-encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2023Quote: ... were transfected with wild-type human NOPR or NOPLight (0.8 µg DNA) and β-arrestin- 2-mCherry (1 µg DNA) using 3 µL Lipofectamine 2000 (Thermo Fisher). After 24 h ...
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bioRxiv - Cell Biology 2022Quote: ... and then blocking in Superblock Blocking Buffer (Thermo Fisher Scientific). Subsequently ...
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bioRxiv - Cell Biology 2024Quote: ... Blocking was performed using BlockAid Blocking Solution (Thermo Fisher Scientific). Primary antibody incubation against clathrin heavy chain (ab21679 from Abcam ...
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bioRxiv - Molecular Biology 2022Quote: ... Blocking Buffer (Invitrogen Cat# 00-4952-54 ...
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bioRxiv - Immunology 2022Quote: ... Proteins were detected on a Li-Cor Odyssey Blot Imager using an anti-Caspase-11 primary antibody (cone 17D9) and Alexfluor-680 conjugated secondary antibody (Invitrogen).
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bioRxiv - Cell Biology 2023Quote: ... each α-arrestin-containing lysate was incubated with GFP-nanobody-conjugated to Dynabeads M-270 Epoxy magnetic beads (#14301, Thermo Fisher Scientific). The supernatant was separated from the beads using a magnetic rack ...
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bioRxiv - Microbiology 2021Quote: ... and blocking in SuperBlock Blocking Buffer (Thermo Fisher Scientific, Waltham, MA). Cleaved caspase-3 was immunostained by incubating HAE with a primary human cleaved caspase-3 (Asp175 ...
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bioRxiv - Cancer Biology 2019Quote: ... After blocking with SuperBlock T20 (PBS) Blocking Buffer (Thermo Fisher Scientific), the plates were incubated with culture supernatant with subsequent 1:2000 diluted peroxidase-conjugated anti-mouse IgG (Agilent Technologies) ...
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bioRxiv - Neuroscience 2023Quote: ... before blocking in SuperBlock™ blocking buffer (Thermo Fisher Scientific, 37515). Primary antibodies (MAP2 (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... followed with endo-biotin blocking using a blocking kit (Invitrogen, #E21390) according to the manufacture instructions [118] ...
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bioRxiv - Microbiology 2023Quote: ... blocking was performed overnight using SEA BLOCK Blocking buffer (ThermoFisher, 37527) at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... The peptide concentrations of eluted peptides were quantified using the Pierce Fluorometric Peptide Assay (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were quantified with Quantitative Colorimetric Peptide Assay (Thermo Scientific) and 5 µg of total peptides were labeled using TMTpro16-plex reagents (Thermo Scientific) ...
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bioRxiv - Developmental Biology 2023Quote: ... Peptides were desalted with peptide desalting spin columns (Thermo Scientific) and dried via vacuum centrifugation ...
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bioRxiv - Cancer Biology 2022Quote: ... A 40-minute blocking step with Super Block Blocking buffer (Thermo Scientific) was performed prior to adding the primary antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... A 40-minute blocking step with Super Block Blocking buffer (Thermo Scientific) was performed prior to adding the primary antibody ...
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bioRxiv - Biochemistry 2023Quote: Peptide samples were assayed for peptide concentration with Pierce™ Quantitative Colorimetric Peptide Assay (Thermofisher Scientific, Brisbane, Australia), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Labelled peptides were quantified (Pierce Colorimetric Peptide Assay Kit, Thermo Scientific) and mixed 1:1:1 based on peptide amount.
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bioRxiv - Molecular Biology 2023Quote: ... Peptides were quantified using a colorimetric peptide assay (Thermo Fisher Scientifc). Peptides (1 µg ...
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bioRxiv - Cell Biology 2024Quote: Peptide concentration was determined using colourimetric peptide assay (Thermo Fisher Scientific). Briefly ...
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bioRxiv - Cell Biology 2024Quote: Peptide concentration was determined using a colorimetric peptide assay (ThermoFisher Scientific). All samples were reconstituted in loading buffer (5% CH3CN ...
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bioRxiv - Biophysics 2021Quote: ... blocking of endogenous biotin was performed using a commercial blocking kit (Invitrogen, E21390). Cells were incubated in component A for 45 minutes ...
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bioRxiv - Developmental Biology 2019Quote: ... After blocking with 10% goat serum or Superblock blocking buffer (Thermo Fisher Scientific) for 30 minutes ...
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bioRxiv - Microbiology 2021Quote: ... Peptide digest assay standard (PierceTM Quantitative Colorimetric Peptide Assay, Thermo Scientific, USA) was as standard for quantitative analysis of peptides ...
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bioRxiv - Cell Biology 2022Quote: ... peptide concentration was measured using a colorimetric peptide quantification assay (Thermo Scientific). Finally ...
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bioRxiv - Systems Biology 2019Quote: ... Peptides were quantified using Pierce Quantitative Fluorometric Peptide Assay (Thermo Fisher Scientific) according to manufacturer’s instructions on a Varioskan LUX Multimode Microplate Reader and SkanIt 5.0 software (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... Peptide concentration was determined by a quantitative colorimetric peptide assay (Thermo Fisher), and 1 μg aliquots of clean peptides from each sample were injected sequentially over a nano-flow C18 column (EasySpray ...
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bioRxiv - Cell Biology 2022Quote: ... Peptide concentrations were determined using Pierce Quantitative Fluorometric Peptide Assay (ThermoFisher Scientific).
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bioRxiv - Cell Biology 2021Quote: ... peptides were desalted on PierceTM Peptide Desalting Spin Columns (Thermo Fisher Scientific) and concentrated in a SpeedVac concentrator.
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bioRxiv - Microbiology 2021Quote: ... The peptide digests were then cleaned up using peptide desalting columns (ThermoFisher) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Cell suspensions were placed in clean cytospin cones and spun at 600g for 5 min on an Epredia Cytospin 4 (Fisher Scientific Cat#A78300003) to charged glass slides ...
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bioRxiv - Immunology 2021Quote: ... After washing with PBS-T and blocking with StartingBlock (TBS) blocking buffer (ThermoFisher Scientific), serially diluted serum from groups of immunized mice or baboons were added to coated wells and incubated for 1 hour at room temperature ...
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bioRxiv - Immunology 2023Quote: ... followed by blocking with Starting Block T20 blocking buffer (Thermo Fisher Scientific catalog #37539) for 13 minutes ...
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Pan-tissue mitochondrial phenotyping reveals lower OXPHOS expression and function across tumor typesbioRxiv - Biochemistry 2023Quote: ... following peptide quantification (ThermoFisher). All samples were subjected to nanoLC-MS/MS analysis using an UltiMate 3000 RSLCnano system (Thermo Fisher ...
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bioRxiv - Genomics 2023Quote: Custom peptides (ThermoFisher Scientific) with the sequence N-CAIHAK115RVTIMPK-C were synthesized as unmodified (H3K115) ...
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bioRxiv - Immunology 2022Quote: ... Casein blocking buffer (Thermo Scientific) was then added and the cells were incubated for 60 to 90 minutes at 37 °C in 10% CO2 ...
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bioRxiv - Microbiology 2023Quote: ... SEA blocking buffer (Thermo Scientific) and 1% BSA in PBS in the subsequent panning rounds ...
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bioRxiv - Plant Biology 2020Quote: ... Tryptic peptide samples were quantified by a colorimetric peptide assay (Pierce, Thermo Fisher) and aliquots of 200 ng were injected by LC-MS/MS ...
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bioRxiv - Developmental Biology 2020Quote: ... peptide concentrations were quantitated by the Pierce Quantitative Colorimetric Peptide Assay (Thermo Fisher).