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Citations for Thermo Fisher :
251 - 300 of 10000+ citations for Coiled Coil Domain Containing 28B CCDC28B Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... Cells were washed and incubated for 15 minutes in binding buffer containing 0.5 μM goat anti-mouse AF488-labeled antibody (ThermoFisher) and streptavidin-conjugated AF647 ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by 1 h incubation with 1:1,000 anti-mouse Alexa Fluor 488 conjugated secondary antibody (diluted with PBS containing 5% BSA, Invitrogen) at RT in the dark ...
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bioRxiv - Immunology 2020Quote: ... Cells were then subsequently stained with an antibody cocktail containing LIVE/DEAD Fixable Dead Cell Stain Kit (Thermo Fisher), and surface stained with the antibodies listed in S2-S4 Tables for 30 minutes at 4°C ...
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bioRxiv - Genomics 2021Quote: ... nuclei were incubated for 1 hour in a solution containing 5% FBS and 1:500 primary antibody (H3K9me3; Invitrogen rabbit polyclonal 49-1008 or H3K9Ac ...
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bioRxiv - Immunology 2020Quote: ... washed with PBS containing 0.5% BSA and incubated with anti-CD89 PE antibody and Hoechst 33342 (Invitrogen, cat. R17753) for 30 min at 4 °C ...
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bioRxiv - Genomics 2021Quote: ... and incubated with AbDil containing 2 ug/ml Alexa 647 fluorophore conjugated goat anti-rabbit secondary antibody (Molecular Probes) for 30 minutes ...
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bioRxiv - Genetics 2022Quote: ... Samples were transferred to fresh dilution buffer containing secondary antibodies (1:200 Alexa Fluor goat anti-chick 488 (Invitrogen) and 1:200 Alexa Fluor goat anti-mouse 633 (Invitrogen)) ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed with 0.1 M PBS containing 0.3% triton X-100 then incubated in secondary antibodies (donkey anti-rabbit Alexa-fluor 594, Invitrogen A21207 and donkey anti chicken Alexa-fluor 647 ...
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bioRxiv - Genomics 2023Quote: ... Chromosomes were stained with anti-Elys antibodies [65] (1:400) diluted in PBT containing 0.3% Triton X-100 and 3% Normal Goat Serum (Invitrogen) and counterstained with Dapi ...
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bioRxiv - Immunology 2023Quote: ... cells were washed twice and resuspended in PBS containing 1:10,000 diluted goat anti-rabbit IgG-647 antibody (Invitrogen). After 30mins at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... the coverslips were washed 4 times with PHEM containing 0.05% Tween-20 and incubated with appropriate Alexa Fluor 633-conjugated secondary antibodies (Goat anti-Mouse, Invitrogen A21052 or Goat anti-Rabbit ...
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bioRxiv - Cancer Biology 2023Quote: ... Coverslips were incubated in PBS containing both goat anti-rat 647 secondary antibody (1:500; Cat# A-21247, ThermoFisher) and goat anti-mouse 488 secondary antibody (1:500 ...
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bioRxiv - Immunology 2023Quote: ... Cells were then stained in the appropriate antibody master mix containing 1:1000 eFluor 780 Fixable Viability Dye (Invitrogen) and 1:500 Fc block (Prof ...
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bioRxiv - Cancer Biology 2023Quote: ... the remaining cells were stained for sorting in PBS containing 0.5% FCS at 4L°C with the following antibodies: Streptavidin-AF488 (1:100, Invitrogen), CD45-BV510(1:50 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed thrice in 1X PBS containing 1.8% NaCl before incubating in Alexa Fluor conjugated secondary antibodies (ThermoFisher), again overnight ...
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bioRxiv - Biochemistry 2024Quote: ... cell suspensions were incubated for 20 min at 4°C in 80 µL of L15 medium containing 2.1 µg of anti-rat CD2 antibody (Invitrogen) per 15 million dissociated cells ...
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bioRxiv - Neuroscience 2024Quote: ... spinal cord sections were incubated in 0.1% PBS-T solution containing primary antibodies and 10% heat-inactivated horse serum (Invitrogen). The primary antibodies used in this study are listed in the Table 1 ...
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bioRxiv - Neuroscience 2024Quote: ... sections were washed in TBS then incubated in blocking buffer containing secondary antibodies (Alexa Fluor conjugates, 1:400, Invitrogen) for 3-4 h at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... containing growth medium containing 10% FBS (ThermoFisher Scientific, Waltham, MA, USA), 1% penicillin (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... fusion protein containing the RhoA binding domain of rhotekin (rhotekin-GST) was performed by the manufacturer’s protocol using GTPase Pull-Down kit (Thermo Scientific, Waltham, MA, USA). Activated and total RhoA were detected by Western blot analysis using anti-RhoA antibody[15].
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bioRxiv - Biochemistry 2019Quote: For mammalian expression a DNA polynucleotide encoding the transmembrane domain (residues 1-295) of GtACR1 optimized for human codon usage was cloned into the pcDNA3.1 vector (Life Technologies, Grand Island, NY) in frame with an EYFP tag ...
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bioRxiv - Microbiology 2022Quote: ... The presence of recombinant virus was verified by immunostaining with a monoclonal antibody against the RABV nucleoprotein (FujiRebio, Cat# 800–092) and polyclonal antiserum against the SARS CoV/CoV-2 S1 domain (Thermo Fisher, Cat# PA581798). The filtered virus was then used to inoculate Vero cells seeded in Cellstack Culture Chambers (Corning ...
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bioRxiv - Biophysics 2022Quote: The F2F3 lobe (206-405) of Talin FERM domain and full length MBD2 genes were codon optimized for bacterial expression and synthesized by Invitrogen (Thermo Fisher Scientific). Talin F2F3 domain had C336S mutation to improve protein stability and was cloned into a modified pET32a-m vector using the following oligonucleotides 5’-AAAACCTATGGTGTTAGCTTTTTTCTG-3’ and 3’-ATAACTGTAATAAGACTTTTTCTTTTTTAGTATT-5’ then transformed into E ...
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bioRxiv - Immunology 2022Quote: ... and the N-terminal peptidase domain of human ACE2 were expressed using the Bac-to-Bac Baculovirus Expression System (Invitrogen, Carlsbad, CA, USA) and purified by Strep-Tactin Sepharose (IBA Lifesciences ...
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bioRxiv - Biochemistry 2020Quote: The cDNA encoding a fusion protein consisting of GST followed by a 3C cleavage site and the human SMAD2 MH2 domain (residues 241–465) was inserted into the MCSI of the pFastBac™ Dual vector (Thermo Fisher Scientific) in the Sal1 and Spe1 restriction sites ...
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bioRxiv - Immunology 2021Quote: The SARS-CoV-2 receptor-binding domain (RBDwt) and the RBD mutant (RBDmut) (K417N, E484K, N501Y) were expressed using Expi293F cells (Invitrogen, ThermoFisher Scientific, MA, USA). The genes that encode SARS-CoV-2 RBDwt (residues Arg319-Phe541 ...
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bioRxiv - Immunology 2021Quote: The SARS-CoV-2 receptor-binding domain (RBDwt) and the RBD mutant (RBD417, RBD501, RBD484 and RBDtrip) were expressed using Expi293F cells (Invitrogen, ThermoFisher Scientific, MA, USA). The genes that encode SARS-CoV-2 RBDwt (residues Arg319-Phe541 ...
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bioRxiv - Plant Biology 2022Quote: ... It was then transferred to the pLAW10 vector (with the GAL4 DNA-binding domain) with the LR cloning Gateway strategy (Thermo Fisher, http://www.thermofisher) and used as bait ...
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bioRxiv - Molecular Biology 2022Quote: ... Wells were coated with 1 μg Gfp for anti-GfpNB or 1 μg commercially available SARS-CoV-2 Spike-RBD-domain protein for SARS-CoV-2 nanobody-Cts1-fusion proteins (Invitrogen, Waltham Massachusetts, USA). Recombinant Gfp was produced in E ...
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bioRxiv - Molecular Biology 2023Quote: Ligand-induced recruitment of SRC-1 coactivator peptide to the ligand binding domain of FXR was assayed by time-resolved FRET using LanthaScreen technology according to the manufacturer’s instructions (Thermo Fisher Scientific, cat# A15140). Concentration of test compounds ranged from 10 nM to 100 μM in 1.0% DMSO ...
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bioRxiv - Bioengineering 2023Quote: ... 5 μM of both sensor domains were mixed with different concentrations of Gln or other amino acids in the Nunc Microwell 96-well Microplate (Thermo fisher, MA, US), and the mixture was incubated in the dark for 1–5 h at 22 °C ...
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bioRxiv - Biophysics 2021Quote: ... followed by 1 h incubation with 1:1000 anti-mouse Alexa Fluor 488 conjugated secondary antibody (diluted with PBS containing 5% BSA, Invitrogen) at RT in the dark ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated in a secondary antibody solution containing 1:250 donkey anti-mouse ALEXA 488 (Invitrogen, Burlington, ON, Canada) or donkey anti-goat ALEXA 488 (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... the following was then added to each tube containing 1.2ml of nuclei: 5ul anti-GFP antibody (Thermo Fisher Scientific G10362), 200 ul Wash Buffer ...
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bioRxiv - Neuroscience 2021Quote: ... Reactions with primary antibodies were performed in blocking buffer containing rabbit anti-GFP (1:1000, Invitrogen, Carlsbad, CA, USA, A11122) and/or goat anti-c-Fos (1:1000 ...
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bioRxiv - Genomics 2020Quote: ... Cells were washed three times with 0.01% Triton X-100 in PBS for 5 minutes each and then incubated in blocking solution containing corresponding secondary antibodies labeled with Alexa fluorophores (Invitrogen) for 1 hour at room temperature ...
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bioRxiv - Immunology 2021Quote: ... All stains were carried out in media containing anti-CD16/32 blocking antibody (ThermoFisher, clone 93, catalog # 14-0161-86). All flow cytometry was acquired on an LSRFortessa FACS analyzer ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were then washed in PBS and treated with a secondary antibody incubation containing donkey anti-rabbit AlexaFluor 555 (1:500; Invitrogen), donkey anti-goat AlexaFluor 647 (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... the tissue was incubated in blocking solution containing secondary antibodies for 24 hr (streptavidin AlexaFluor conjugate, Invitrogen; 1:250 goat anti-mouse AlexaFluor conjugate, Invitrogen). Following staining ...
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bioRxiv - Neuroscience 2020Quote: ... incubated in dilution media containing a 1:400 dilution of Alexa Fluor 488 goat anti-rabbit antibody (Invitrogen, Carlsbad, CA) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... sections were again incubated at room temperature for 3 hours in TBS containing an Alexa Fluor 546-conjugated donkey anti-goat IgG secondary antibody (1:400, A-11056, ThermoFisher), 0.5% Triton X-100 ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then washed three times in PBS and incubated with PBS containing 3% BSA and secondary antibodies (Alexa 488 goat anti-mouse; Thermofisher) for 1 hour at room temperature ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... for two days followed by incubation in a solution containing Alexa-Fluor Plus 594 donkey anti-rabbit antibody (1:3000; A21207, Invitrogen) for 2 hrs.
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... overnight followed by incubation in a solution containing a secondary Alexa-Fluor Plus 488 donkey anti-rabbit antibody (1:3000; A21206, Invitrogen) for 2 hrs.
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bioRxiv - Biochemistry 2022Quote: ... in PBS containing 1 mM CaCl2 and 1 mM MgCl2 were stained separately with either FITC-conjugated anti-CD18 antibody MEM48 (Thermofisher) at 1:100 dilution or APC-conjugated CBRM1/5 at 0.5 μg/mL ...
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bioRxiv - Developmental Biology 2022Quote: ... The secondary antibodies (1:300 diluted in PBST containing 10% FBS) were Alexa Fluor 488 goat anti-mouse IgG (A21121; Invitrogen), Alexa Fluor 488 goat anti-rabbit IgG (A11034 ...
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bioRxiv - Neuroscience 2019Quote: ... the tissue was then placed in blocking solution containing secondary antibodies for 24 hr (streptavidin AlexaFluor conjugate from Invitrogen, 1:250 goat anti-mouse AlexaFluor conjugate from Invitrogen). To label muscles ...
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bioRxiv - Neuroscience 2020Quote: ... in PBS containing 0.3 % Triton X-100 and incubated overnight at 4°C with a rabbit anti-Iba1 antibody (1:500, Wako) in PBS containing 1 % FBS (Gibco), 0.1 % BSA (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were transferred to wells containing primary antibodies (1:5000 rabbit anti-c-Fos [SySy]; 1:500 chicken anti-GFP [Invitrogen]) and allowed to incubate on a shaker overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: Surface antibody staining was performed in PBS containing 5% FCS and 1mM EDTA and using a Fixable Aqua Dead Cell (Invitrogen) to determine viability ...