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Citations for Thermo Fisher :
601 - 650 of 10000+ citations for Cleaved PARP Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... 5250 ng/mL human recombinant IL-6 protein (Invitrogen; Waltham, MA) was added to the experimental group and an equal volume of 1X PBS was added to the control group ...
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bioRxiv - Biochemistry 2024Quote: ... Recombinant murine pro-CtsK were expressed in 293-Freestyle cells (ThermoFisher) by transient expression using FectoPRO transfection reagent (Polyplus transfection) ...
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bioRxiv - Synthetic Biology 2023Quote: All recombinant proteins were produced in Expi293F cells (Thermo Fisher Scientific) by transient transfection ...
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bioRxiv - Biophysics 2024Quote: ... The recombinant DR2539 was expressed in Escherichia coli BL21 (DE3) (Invitrogen). The cells were grown at 310 K to an OD600 of ≈ 0.6 in Luria-Bertani medium containing 100 µg ml-1 of ampicillin ...
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bioRxiv - Immunology 2024Quote: ... injections also contained 1 μg of recombinant murine IL-2 (Gibco). PBS was used as vehicle.
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bioRxiv - Immunology 2024Quote: ... used for recombinant protein production were maintained in FreeStyle (ThermoFisher, #12338026) or Expi293 media (ThermoFisher ...
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bioRxiv - Cancer Biology 2024Quote: ... 1% penicillin/streptomycin and 50ng/ml recombinant mouse EGF (Life Technologies) was used for culturing colon organoids ...
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bioRxiv - Immunology 2024Quote: ... Recombinant proteins were diluted in DPBS (Life technologies cat. # 14040-182) (tetNA was diluted to 0.5 µg/ml) ...
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bioRxiv - Cell Biology 2022Quote: ... The cleaved RNA fragments were then copied into first-strand cDNA using reverse transcriptase (SuperScript II, ref. 18064-014, Invitrogen) and random primers ...
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bioRxiv - Cell Biology 2023Quote: ... The cleaved RNA fragments are copied into first strand cDNA using SuperScript II reverse transcriptase (#18064014, Invitrogen, Thermo Fisher Scientific) and random primers ...
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bioRxiv - Cell Biology 2023Quote: ... The cleaved RNA fragments are copied into first strand cDNA using SuperScript II reverse transcriptase (#18064014, Invitrogen, Thermo Fisher Scientific) and random primers ...
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bioRxiv - Cell Biology 2020Quote: ... PRC1 (mouse mAb IgG2b, clone 16F2, product MA1-846; used at 1/100) was from ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... Primary Strep-MAb classic chromeo-488 (IBA) and secondary Alexa-fluor 488 goat anti-mouse (Invitrogen) were applied sequentially with PBS washes in between ...
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bioRxiv - Immunology 2021Quote: ... Serial dilutions of the mAb samples were made in 1X minimal essential medium (MEM; Life Technologies) starting at 30 µg/ml ...
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bioRxiv - Microbiology 2021Quote: ... 5 ul of mAb DG52 pre-coupled to 100 μl Dynabeads™ Protein G (Thermo Fisher) was added to the supernatant and incubated for 1 hr at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... Chambers were washed 3X with MAB and incubated with anti-mouse Alexa647 (A21236, Thermo Fisher Scientific) secondary antibody for 1.5 hours ...
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bioRxiv - Microbiology 2020Quote: ... The mouse mAb against cathepsin L (BMS1032) and α-tubulin (T5158) were bought from Thermo Fisher Scientific and Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Goat anti-rat and streptavidin Molecular Probes Alexa-Fluor-488 and 633 conjugated secondary mAbs (Invitrogen) were used at 2µg/ml and all sections were counterstained with DAPI to distinguish cell nuclei ...
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bioRxiv - Immunology 2021Quote: The IgGs representing the mAbs were produced in either HEK 293T (ATCC) or Expi293 (Thermo Scientific) cells ...
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bioRxiv - Microbiology 2021Quote: ... monoclonal anti-HAtag antibody (1:8000; HA Tag mAb 2-2.2.14, Thermo Fisher Scientific, Planegg, Germany) or COVID-19 patient serum (1:200) ...
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bioRxiv - Immunology 2021Quote: ... The IC50 of the evaluated mAbs was tested by the Varioskan LUX Microplate Spectrophotometer (Thermo Fisher), and calculated by a four-parameter logistic regression using GraphPad Prism 8.0.
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bioRxiv - Cell Biology 2022Quote: ... The rat PE-conjugated mAb against F4/80 (catalog #12-4801-82) was from Thermo Fisher. The rabbit polyclonal anti-mouse αM antibody (catalog #ab128797 ...
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bioRxiv - Microbiology 2023Quote: ... followed by an incubation with mouse anti-human E-cad cytoplasmic domain mAb (4A2C7, Life Technologies). In some experiments ...
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bioRxiv - Immunology 2023Quote: ... Myc mAb (D84C12) was labeled by Alexa Fluor™ 647 Antibody Labeling Kit (Invitrogen, Cat# A20186). Surfaced staining was conducted using Cell Staining Buffer (Biolegend ...
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bioRxiv - Bioengineering 2024Quote: ... Tregs were stained with conjugated mAb targeting the intracellular Helios (eBiosciences, Life technologies corp., Carlsbad, CA) and FoxP3 proteins (BD Biosciences) ...
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bioRxiv - Immunology 2024Quote: ... mAbs were expressed by transient transfection in Expi293F cells in FreeStyle F17 expression media (Thermo Fisher) (0.1% Pluronic Acid F-68 and 20% 4 mM L-glutamine ...
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bioRxiv - Immunology 2024Quote: ... AnnexinV/7ADD (eBiosciences) and Fas Ligand Ms Anti-Hu mAb-FITC (SB93a, 1:200, Life Technologies). For the characterization of primary macrophages ...
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bioRxiv - Immunology 2023Quote: ... Anti-flavivirus E protein mAb 4G2 was conjugated to Alexa Fluor 488 5-SDP (Life Technologies) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Morphologically similar yeasts with identical ITS PCR product lengths underwent cleaved amplified polymorphic sequence (CAPS) analysis by digesting the ITS PCR product with TaqI (Thermo scientific) according to the manufacturer’s instructions and separating the fragments on a 3% agarose gel ...
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bioRxiv - Cell Biology 2022Quote: ... The cleaved RNA fragments were then reverse-transcribed to create the cDNAs using SuperScript™ II Reverse Transcriptase (Thermo Fisher Scientific), which were next used to synthesize U-labeled second-stranded DNAs with E ...
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bioRxiv - Biophysics 2019Quote: ... Peptide amides were cleaved from the resin with 95% (v/v) aqueous trifluoroacetic acid solution (TFA, Fisher Scientific, Loughborough, United Kingdom) containing 5% (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... Protease and cleaved GST was removed from solution by passing the dialyzed and cleaved protein solution over a 10 mL Hispur Ni-NTA gravity column (Thermo Fisher) three times ...
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bioRxiv - Cancer Biology 2023Quote: ... and TNFα (10 ng/ml) for 6 h and stained for cleaved Caspase 3/7 green (5 µM) and propidium iodide (2 µM) (Thermo Scientific) for an additional 30 min ...
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bioRxiv - Plant Biology 2019Quote: ... The recombinant enzymes were probed using anti-V5-HRP conjugated antibody (Invitrogen), which was detected using an ECL Advance Western Blotting Detection Kit (Amersham ...
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bioRxiv - Immunology 2021Quote: ... and recombinant proteins were purified using Ni-NTA agarose (Thermo Fisher Scientific), then buffer-exchanged into PBS and concentrated using Amicon Ultra centrifugal filters (MilliporeSigma) ...
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bioRxiv - Molecular Biology 2020Quote: Recombinant 6x-His tagged PTB was purified using Ni-NTA agarose (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... Recombinant BUD13 purification was confirmed by SimplyBue SafeStain (Thermo Fisher Scientific, LC6060) and western blot using BUD13 antibody (Bethyl Laboratories ...
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bioRxiv - Immunology 2021Quote: All recombinant proteins were expressed and purified using Expi293F cells (Life Technologies,) as described in detail previously [34 ...
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bioRxiv - Immunology 2019Quote: ... slices were overlaid with 50ng recombinant IL-15/IL-15Rαcomplex (Thermo Fisher) in 10□1 of cRPMI following peptide treatment ...
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bioRxiv - Biochemistry 2019Quote: ... Recombinant virus was made by co-transfection into SF9 insect cells (Invitrogen) of the plasmid and BacVector3000 baculovirus DNA (Novagen ...
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bioRxiv - Molecular Biology 2019Quote: ... and 2) 1 U/μL RNaseOUT™ Recombinant Ribonuclease Inhibitor (ThermoFisher Scientific). Hydra polyps in MCB buffer were homogenized on ice using a Dounce homogenizer ...
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bioRxiv - Neuroscience 2019Quote: ... and 20 ng/mL recombinant human fibroblast growth factor-basic (Life Technologies). After 3 to 6 weeks ...
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bioRxiv - Immunology 2019Quote: ... recombinant macrophage colony-stimulating factor (100 U/ml; ebioscience, Thermo Fisher Scientific) and Pen Strep (1:100 dilution ...
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bioRxiv - Cell Biology 2019Quote: ... 20 ng/ml recombinant mouse macrophage colony-stimulating factor (Gibco, Burlington, ON), and penicillin/streptomycin antibiotics ...
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bioRxiv - Microbiology 2021Quote: ... 20 ng/ml of recombinant human epidermal growth factor (EGF, Life Technologies), human insulin (Sigma) ...
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bioRxiv - Developmental Biology 2021Quote: ... and 10 ng/ml bFGF recombinant human protein (Thermo Fisher Scientific, USA). From day 6 until day 12 the medium was changed once in two days with the addition of 1 mM valproic acid (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... The recombinant plasmid was linearized using the Ncol restriction enzyme (ThermoFisher Scientific) and the MEGAscript® T7 Kit (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... and 20 U/ml recombinant interleukin-2 (IL-2; Thermo Fisher Scientific) at 37° C ...
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bioRxiv - Molecular Biology 2021Quote: ... The guide RNAs and recombinant Cas9-NLS protein (Thermo Fisher Scientific, B25642) were transfected into HEK293 T-Rex cells with Lipofectamine CRISPRMAX Cas9 Transfection Reagent (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: Recombinant baculovirus was generated using the Bac-to-Bac expression system (Invitrogen) according to the manufacturer’s instructions ...