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Citations for Thermo Fisher :
551 - 600 of 3412 citations for Chikungunya virus lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Lysates were mixed in 4x LDS loading buffer containing DTT (ThermoFisher) and heated to 70°C for 10 min ...
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bioRxiv - Systems Biology 2020Quote: ... The protein lysates were mixed with 4xNuPAGE LDS Sample Buffer (Invitrogen) supplemented with 10% 2-mercaptoethanol (Roth) ...
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bioRxiv - Cell Biology 2021Quote: Lysates were mixed with 4x NuPAGE LDS Sample Buffer (ThermoFisher NP0008) and electrophoresed through AnyKD precast gels (BioRad 4569034 ...
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bioRxiv - Cell Biology 2021Quote: ... Protein lysate concentration was estimated using a BCA assay (ThermoFisher Scientific). 15 μg protein was resolved on 4-12% Bis-Tris polyacrylamide gel (Invitrogen ...
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bioRxiv - Biophysics 2021Quote: ... Clarified lysate was bound to His-Pur Cobalt resin (Thermo Scientific) for 2 hours with rocking at 4°C ...
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bioRxiv - Biophysics 2020Quote: ... The cell lysate was centrifuged (Heraeus, Biofuge, Stratos, Thermo Fisher Scientific) at 10,000 rpm at 4°C for 1 hour ...
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bioRxiv - Cancer Biology 2021Quote: ... The lysates were mixed with LDS sample buffer (Life Technologies, NP0008) and sample reducing agent (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... The lysates were precleared with 10µL ProteinA magnetic beads (10008D, ThermoFisher) for 1 h at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... and the clarified lysate was transferred to protein G Dynabeads (Invitrogen) conjugated to the monoclonal anti-LIS1 antibodies for two biological replicates ...
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bioRxiv - Neuroscience 2022Quote: HeLa cell lysates were prepared on ice using RIPA buffer (Thermofisher) containing protease and phosphatase inhibitors (Roche) ...
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bioRxiv - Neuroscience 2022Quote: ... Immunoprecipitation from cell lysates was performed using Dynabeads Protein A (Thermofisher) following the manufacturer ’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: Total protein lysates were extracted with RIPA buffer (Thermo Scientific, 89901) supplemented with protease inhibitor cocktail (Roche ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein lysates were quantified by Pierce BCA assay (Thermo Scientific, 23222). Protein samples were diluted in 4x sample buffer (Bio-rad ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein lysates were quantified by BCA method (PI23227, Thermo Fisher Scientific) and equal amounts were used for subsequent assays ...
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bioRxiv - Molecular Biology 2022Quote: ... 15 µL of lysates were mixed with sample buffer (Invitrogen NP0008) before being incubated at 100°C for 5-10 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein concentration of the lysate was determined by BCA assay (Thermofisher). Proteins (20–30μg ...
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bioRxiv - Immunology 2022Quote: ... Lysates were cleared by Protein A/G Agarose (Thermo Fisher Scientific) for 1 hour and followed by immunoprecipitated with primary antibody of PP2B-Aα ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were pre-cleared with Protein G magnetic beads (Thermo Fisher) for 30 min at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Lysate samples were diluted to 1 μg/μl with DPBS (GIBCO) and spectra analyses were done with 1.5 μl on a Nanodrop Spectrophotometer (Implen ...
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bioRxiv - Biochemistry 2022Quote: ... Protein content of lysate was quantified using BCA assay kit (ThermoFisher). Whole cell lysates or purified PDHC reactions were separated on 4-12% Bolt Gel (ThermoFisher ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate protein concentrations were determined using the BCA Assay (Thermo Fisher) and normalized prior to SDS-PAGE ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were denatured and reduced using LDS sample buffer (Invitrogen, NP0007) containing 5% beta-mercaptoethanol and heated at 70-95°C for 5-10 mins.
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bioRxiv - Cancer Biology 2021Quote: Cell lysates were generated using lysis buffer (Thermo Fisher Scientific, 1862301) containing protease inhibitor cocktail (Cell Signaling Technology ...
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bioRxiv - Microbiology 2021Quote: ... Protein lysates were run on 4%–12% Bis-Tris gels (Invitrogen), transferred onto PVDF membranes (Bio-Rad) ...
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bioRxiv - Cancer Biology 2021Quote: ... Total protein lysate was combined with NuPAGE LDS Sample Buffer (Invitrogen) and NuPAGE Sample Reducing Agent (Invitrogen) ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysates were pre-cleaned with 20 μl of dynabeads (Life Technologies) per sample for 1 h at 4 °C with rotation ...
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bioRxiv - Neuroscience 2020Quote: ... lysates were precleared with 40 μl of Protein G agarose (Invitrogen) for 30 min at 4 °C and then were incubated overnight with anti-GlyT2 ...
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bioRxiv - Molecular Biology 2022Quote: ... Lysates were then incubated with HisPur Cobalt Resin (Thermo Fisher # 89965) in a Peirce centrifugation column (Thermo # 89897 ...
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bioRxiv - Microbiology 2019Quote: ... Lysates were incubated with Dyna-beads Protein A magnetic beads (Invitrogen) coated with rabbit-raised affinity-purified antibodies directed against RocC (38) ...
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bioRxiv - Synthetic Biology 2019Quote: ... phage lysates were treated with TURBO DNase I (Thermo Fisher: AM2238) according to manufacture protocol to eliminate any soluble plasmid ...
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bioRxiv - Physiology 2019Quote: Total RNA was extracted from whole kidney lysates with Trizol (Invitrogen). cDNA synthesis and PCR amplification were performed using PrimeScript First-Strand cDNA Synthesis kit (TaKaRa Bio ...
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bioRxiv - Neuroscience 2019Quote: Lysates were separated by SDS-PAGE (4-12% Bis-Tris, Invitrogen) and transferred to PVDF membrane and probed with antibodies against TrkB (1:1000 ...
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bioRxiv - Immunology 2020Quote: ... 15 µg of lysates were reduced in NuPAGE reducing reagent (Invitrogen) and boiled for 5 min prior to loading in 4-12% NuPAGE Bis-Tris Gel for separation by electrophoresis ...
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bioRxiv - Microbiology 2021Quote: ... RNA was collected from cell lysate with Trizol reagent (Invitrogen #15596018) and extracted with Direct-zol RNA Miniprep Plus kit (Zymo #R2072) ...
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bioRxiv - Microbiology 2021Quote: ... Clarified lysates were loaded on Ni-NTA resin columns (Thermo Scientific) previously equilibrated with 6% (v/v ...
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bioRxiv - Genomics 2021Quote: ... 200ug of cell lysates (in Pierce IP lysis buffer; #87787, ThermoFisher), derived from fully differentiated CAD or N2a cells ...
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bioRxiv - Microbiology 2021Quote: Whole cell lysates were prepared in RIPA lysis buffer (ThermoFisher Scientific). After sonication for 30 seconds ...
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bioRxiv - Biochemistry 2021Quote: ... the lysate was batch bound to Ni-NTA affinity resin (ThermoFisher), the resin was washed with lysis buffer ...
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bioRxiv - Bioengineering 2021Quote: Lysate proteins: RIPA lysis and extraction buffer (Thermo Fisher Scientific 89900) supplemented with halt protease & phosphatase inhibitor cocktail (Thermo Fisher Scientific 78440 ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein concentration of the lysate was determined by BCA assay (Thermofisher). Lysates were resuspended in Laemmli SDS-PAGE sample loading buffer (10% glycerol ...
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bioRxiv - Cancer Biology 2021Quote: ... The concentration of protein lysates was measure by Qubit assay (Invitrogen) before analysis ...
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bioRxiv - Cell Biology 2020Quote: ... The cell lysates were fractionated using 4–12% NuPAGE gels (Invitrogen), immobilized on to PVDF membranes (Millipore ...
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bioRxiv - Cell Biology 2020Quote: ... lysates were applied to Ni-NTA resin (Thermo Scientific, Rockford, IL) that had been equilibrated with lysis buffer containing 20 mM imidazole ...
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bioRxiv - Cell Biology 2019Quote: ... Lysate was sonicated using a Sonic Dismembrator (Model 60; Fisher Scientific) at setting 3.5 with 10 s per pulse for 15 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... total RNA was extracted from lysate powder using TRI Reagent (Ambion) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... Protein concentration of lysates were determined by Bradford Assay (Thermo Scientific).
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bioRxiv - Microbiology 2021Quote: ... Clarified lysates were run on 4%– 12% Bis-Tris gels (Invitrogen), transferred onto PVDF membranes (Bio-Rad) ...
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bioRxiv - Immunology 2020Quote: ... Whole cell lysates were prepared using RIPA lysis buffer (Thermo Scientific) with added protease and phosphatase inhibitors ...
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bioRxiv - Cell Biology 2021Quote: ... Cleared lysate was incubated with 2 mL glutathione-sepharose (Fisher Scientific) for 1 hr and washed with 50 mL (25 CV ...
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bioRxiv - Microbiology 2022Quote: ... The lysate was treated with sodium dodecyl sulphate (SDS) (Acros Organics), ethylenediamine-tetraacetic acid (EDTA ...