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Citations for Thermo Fisher :
701 - 750 of 10000+ citations for Centrosomal Protein 63 kDa CEP63 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... lysosomes were also stained using fluorescent 10 kDa Dextran A647 (D22914; Thermo Fisher). Cells were incubated with 0.1mg/mL of Dextran for 3 hours at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... or 70 KDa amino-dextrans (Thermo Fisher D1862 or Fina Biosolutions AD70×33) were solubilized at various concentrations in sterile 0.1 M NaHCO3 buffer adjusted to pH 8.3 and reacted with the following N-hydroxysuccinimidyl esters (NHS) ...
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bioRxiv - Biophysics 2023Quote: ... and 5% polyethylene glycol (PEG) (50% w/w 10 kDa, Thermo Fisher Scientific) in 50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Microbiology 2020Quote: ... Protein bands were detected using a primary antibody followed by an HRP-conjugated secondary antibody (rabbit, Life technologies #G21234, or mouse, Invitrogen #G21040) and visualized via chemiluminescence using Clarity Western ECL Substrate and the ChemiDoc MP Imaging System (BioRad) ...
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bioRxiv - Genomics 2021Quote: ... Membranes with liver and muscle mitochondrial protein samples were incubated overnight with COX4 antibody (1:1.000 COX4 Polyclonal Antibody, cat no. PA5-17511, Thermo Fisher Scientific, Rockford, USA) and loading control protein ATP5A antibody (1:1.000 anti-ATP5A antibody ...
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bioRxiv - Cancer Biology 2021Quote: ... and the rest was added to antibody-coupled Dynabead Protein A (Thermo Fisher Scientific, Cat #10001D) and incubated overnight rocking at 4 °C ...
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bioRxiv - Genetics 2021Quote: ... Epitope-tagged bait proteins were detected with mouse anti-c-myc monoclonal antibody (Invitrogen, MA1-980) and IR-680 conjugated donkey anti-mouse secondary antibody (LI-COR ...
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bioRxiv - Immunology 2021Quote: ... The secondary antibody for the positive controls was Purified RecombTM Protein A/G Peroxidase Conjugated (Invitrogen). Plates were left to incubate with the primary antibody for 2 hours at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Labeled phalloidins and secondary antibodies and the protein molecular weight marker were purchased from Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... Protein-specific antibodies were visualized using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific). The following antibodies were diluted in TBS ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Proteins were detected with horseradish peroxidase-conjugated secondary antibody (1:5,000; goat anti-rabbit (Thermo Fisher Scientific #31460 or 1:3,000 ...
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bioRxiv - Biophysics 2019Quote: ... The antibody was labeled by TexasRed (Texas Red™-X Protein Labeling Kit, ThermoFisher, CA, USA). Labeling efficiency of the antibody was determined as 1.52 TexasRed/antibody ...
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bioRxiv - Genomics 2021Quote: ... antibodies were conjugated to a mix of washed Dynabeads protein A and G (Thermo Fisher Scientific), and incubated on a rotator (overnight at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Antibody-bound chromatin was incubated with 50 ul of magnetic beads (G-protein magnetic Beads, Invitrogen) 2 hrs on rotating platform at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... The membranes were incubated with rabbit anti-SARS coronavirus spike protein polyclonal antibody (Invitrogen, PA1-41513) diluted 1:10,000 ...
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bioRxiv - Systems Biology 2020Quote: ... The concentration of conjugated oligo DNA and antibody with BCA Protein Assay Kit (Thermo Scientific 23225) were quantified using Nanodrop.
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bioRxiv - Immunology 2021Quote: ... Monoclonal antibodies were purified using protein A resin columns for IgG and CaptureSelect beads (Thermo Scientific) for DH1017.IgM following manufacturer’s recommendations ...
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bioRxiv - Microbiology 2021Quote: ... Protein bands were detected with specific antibodies using SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher) and viewed on a FluorChem R system (Proteinsimple).
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bioRxiv - Cancer Biology 2022Quote: ... Antibody-protein complexes were visualized using the Pierce Enhanced Chemiluminescence Western Blotting Substrate (ThermoFisher Cat# 32109). Images were acquired using the ChemiDoc Imaging System (Bio-Rad) ...
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bioRxiv - Neuroscience 2022Quote: ... 10 µl of Dynabeads Protein G or A (depending on the antibody species) (Thermo Fisher Scientific) and 1 µg antibody or IgG control were added to 200 µl lysis buffer and rotated for 30–40 min at RT ...
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bioRxiv - Microbiology 2020Quote: ... anti-NeuN and anti-SARS-CoV-2 spike protein antibodies (Thermo Fisher Scientific, Norcross, GA, USA) overnight at 4°C followed by incubation with Alexa Fluor 546- or Alexa Fluor 488-conjugated secondary antibody for 1 hr at room temperature [31,34] ...
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bioRxiv - Immunology 2021Quote: Monoclonal antibody was digested into Fab using papain resin and purified by protein A beads (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: ... Complexes bound to the antibody were captured with protein G Dynabeads (Thermo Fisher Scientific, Waltham, MA), extensively washed ...
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bioRxiv - Bioengineering 2020Quote: ... Bound patient antibodies were then detected using Protein L-HRP (Thermo Fisher Scientific 32420, [1:5000]). Background from the raw ELISA signal in serum-treated wells was removed by first subtracting the signal measured in NeutrAvidin-alone coated wells then subtracting the signal detected in antigen-coated wells incubated with 1% nonfat milk + 100 nM competitor.
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bioRxiv - Microbiology 2022Quote: ... (ii) the 3’ adapter was ligated to protein/RNA complexes on antibody-conjugated Dynabeads (ThermoFisher 10004D) instead of ligating to purified RNAs ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The antibody-antigen complexes were captured by incubation with Dynabeads™ protein G (Thermo Fisher Scientific) at room temperature for 2 h ...
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bioRxiv - Microbiology 2019Quote: ... Immunoprecipitation was performed using Protein G Dynabeads and Leishmania GP63 monoclonal antibody clone 96-126 (Thermofisher). As instructed by the manufacturer ...
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bioRxiv - Cancer Biology 2019Quote: ... and the antibody-bound material was then precipitated with 30 μl protein-G Sepharose beads (Invitrogen) that had been pre-equilibrated with 30 μg bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2019Quote: ... Antibody-bound protein was visualized using Pierce ECL Western blotting substrate (32106; ThermoFisher Scienific, Waltham, MA). To visualize loading controls ...
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HIV-1 Nef interacts with LMP7 to attenuate immunoproteasome formation and MHC-I antigen presentationbioRxiv - Microbiology 2019Quote: ... cell lysate supernatants were mixed with antibodies and recombinant protein G-Sepharose 4B beads (Thermo Fisher) at 4°C for overnight ...
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bioRxiv - Molecular Biology 2019Quote: ... Antibody-conjugated bead preparation was performed as follows: 120 μl of DynaBeads Protein G (ThermoFisher Scientific) was transferred to a tube and washed twice with IP buffer ...
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bioRxiv - Developmental Biology 2019Quote: ... Protein-antibody complexes were detected following incubation of the membrane with SuperSignal West Femto (Thermo Fisher). Chemiluminescence was detected under UV (VersaDoc ...
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bioRxiv - Plant Biology 2020Quote: ... Serum was used for antibody affinity purification to the STS1 recombinant protein using magnetic beads (Invitrogen). Validation of antibody was carried out by immunoblot with total protein extract from sts1 complemented lines and sts1 mutant as a negative control ...
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bioRxiv - Systems Biology 2019Quote: ... antibodies and proteins were visualized on X-ray film using Super Signal chemiluminescence reagent (Thermo Scientific).
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bioRxiv - Cell Biology 2021Quote: ... Rabbit antibodies against the following proteins were used: α-catenin (Thermo Fisher Scientific 711200; 1:100), pMRLC (Merck Millipore AB3381 ...
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bioRxiv - Microbiology 2020Quote: ... Bound protein was detected via the C-terminal 6XHis Tag using 6XHis Antibody MA1-135 (Invitrogen) followed by three washes in TBS-T and incubation with donkey anti-mouse horseradish peroxidase (HRP)-coupled secondary antibody (Dianova) ...
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bioRxiv - Cell Biology 2021Quote: ... supernatants were subjected to immunoprecipitation with anti-Myc antibody crosslinked to Protein G Dynabeads beads (ThermoFisher) for 2 hrs ...
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bioRxiv - Genomics 2021Quote: ... Cleared cell lysates were subjected to IP with CTCF antibody-bound Protein G Dynabeads (Invitrogen, 10004D) overnight ...
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bioRxiv - Microbiology 2020Quote: ... The lysate/antibody mix was then incubated with 30μL of Dynabeads Protein G (#10003D, ThermoFisher Scientific) for 1 hour at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... The antibody-containing supernatant was collected and purified using protein G spin columns (Thermo Fisher Scientific). Eluted purified IgG were dialyzed in PBS using a dialysis cassette (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2022Quote: ... The 3-phosphoglycerate kinase (Pgk1) protein was detected using anti-Pgk1 monoclonal antibody (1:30,000; Invitrogen) as a loading control.
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bioRxiv - Genetics 2022Quote: ... The 3-phosphoglycerate kinase (Pgk1) protein was detected using anti-Pgk1 monoclonal antibody (1:30,000; Invitrogen) as a loading control ...
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bioRxiv - Cell Biology 2022Quote: ... Antibody-chromatin complexes were then captured with Dynabeads® Protein A (cat. no. 10002D, Life technologies). The precipitated material was washed twice with Lysis Buffer ...
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bioRxiv - Plant Biology 2022Quote: ... The GFP protein was probed with an anti-GFP antibody at 1:1,000 dilution (G10362, Thermofisher) and visualized using a goat anti-rabbit IgG (H+L ...
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bioRxiv - Plant Biology 2023Quote: ... and proteins were visualized using Western blot and monoclonal anti-ubiquitin antibody (Fisher scientific, eBioP4D1 (p4D1)) ...
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bioRxiv - Molecular Biology 2023Quote: ... Anti-G3BP1 antibody-bound SG cores were collected through the incubation with Protein G Dynabeads (Invitrogen) with consecutive SG RNA elution from the Dynabeads by Proteinase K (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: JD1 antibody was adsorbed to high protein binding Nunc-Immuno MaxiSorp 96-well plates (Nunc, Roskilde,) at 4°C for 4hrs ...
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bioRxiv - Molecular Biology 2022Quote: ... Nucleolar protein 58 (NOP58) was detected using an anti-NOP58 antibody (Thermo Fisher Scientific, PA5-54321) diluted 1:500 and an anti-Rabbit HRP conjugated secondary antibody diluted to 1:10000 ...
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bioRxiv - Cell Biology 2023Quote: ... The eluted proteins were determined by Western blotting with anti-V5 and anti-FLAG antibodies (Invitrogen) and visualized by the horseradish peroxidase-conjugated secondary antibodies (ZSGB-BIO ...
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bioRxiv - Developmental Biology 2023Quote: ... Immunoprecipitations were performed using 1-2ug of antibody bound to protein A/G magnetic beads (Thermofisher), incubated overnight at 4℃ ...