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Citations for Thermo Fisher :
1 - 50 of 375 citations for Canine Coronavirus Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... and coronavirus antigens were expressed in Expi293F cells by transient transfection in FreeStyle F17 expression media (Thermo Fisher) supplemented to a final concentration of 0.1% Pluronic Acid F-68 and 20% 4 mM L-glutamine using Expifectamine transfection reagent (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Coronavirus pan monoclonal antibody (FIPV3-70, Invitrogen) was combined with a secondary antibody using Alexa Fluor 647 fluorophore (10739374 ...
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bioRxiv - Microbiology 2024Quote: ... and TNF-α Canine ELISA Kit (# ECTNF, Invitrogen) were used to measure IL-8 and TNF-α ...
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bioRxiv - Microbiology 2024Quote: ... The IL-8 Canine ELISA Kit (#ECCXCL8, Invitrogen) and TNF-α Canine ELISA Kit (# ECTNF ...
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bioRxiv - Microbiology 2021Quote: ... Madin-Darby Canine Kidney (MDCK) cells were maintained in DMEM (Invitrogen), containing 10% (v/v ...
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bioRxiv - Microbiology 2022Quote: ... and stained using SARS/SARS-CoV-2 Coronavirus Nucleocapsid Monoclonal Antibody (Invitrogen MA5-29981) as the primary antibody followed by Alexa647-conjugated secondary antibody (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: Purified coronavirus spike ectodomains were coated onto 96-well NUNC Maxisorp plates (Thermo Scientific) at equimolar amount at room temperature (RT ...
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bioRxiv - Microbiology 2023Quote: ... First Goat anti-Canine IgG biotin (Thermo Scientific™, Waltham, MA, USA) was added to the fox and wolf samples and antiferret IgG biotin (Merck ...
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bioRxiv - Cell Biology 2020Quote: Madin-Darby canine kidney (MDCK) strain II was cultured in DMEM medium (Invitrogen) supplemented with 10% FBS (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: The Madin-Darby canine kidney (MDCK) cell line was maintained in DMEM (Gibco) supplemented with 10% FBS (Gibco ...
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bioRxiv - Microbiology 2020Quote: ... The membranes were incubated with rabbit anti-SARS coronavirus spike protein polyclonal antibody (Invitrogen, PA1-41513) diluted 1:10,000 ...
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bioRxiv - Neuroscience 2023Quote: ... Antigen retrieval was performed with Low pH IHC Antigen Retrieval Solution (Invitrogen) in a pressure cooker set to 100°C for 10 minutes ...
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bioRxiv - Microbiology 2021Quote: Madin-Darby Canine Kidney (MDCK) cells (ATCC) were maintained in DMEM (Gibco-Invitrogen, Inc.) supplemented with 10% foetal bovine serum (Biosera ...
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bioRxiv - Microbiology 2021Quote: Madin-Darby Canine Kidney (MDCK) cells (ATCC) were maintained in DMEM (Gibco-Invitrogen, Inc.) supplemented with 10% foetal bovine serum (Biosera ...
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bioRxiv - Microbiology 2023Quote: ... Madin-Darby Canine Kidney (MDCK) cells (ATCC) were maintained in DMEM (Gibco-Invitrogen, Inc.) supplemented with 10% foetal bovine serum (Biosera ...
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bioRxiv - Microbiology 2023Quote: ... Madin-Darby Canine Kidney (MDCK) cells (ATCC) were maintained in DMEM (Gibco-Invitrogen, Inc.) supplemented with 10% foetal bovine serum (Biosera ...
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bioRxiv - Cancer Biology 2023Quote: ... antigen retrieval was performed using IHC Antigen Retrieval Solution (ThermoFisher, 00-4955-58) in a steamer for 40Dminutes ...
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bioRxiv - Molecular Biology 2021Quote: ... Madin-Darby canine kidney cells (MDCK) were cultured in Glasgow’s Modified Eagle Medium (GMEM; Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2019Quote: Mardin-Darby Canine Kidney cells (MDCKs) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco) supplemented with L-glutamine and 10% Fetal Calf Serum ...
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bioRxiv - Microbiology 2022Quote: Madin-Darby canine kidney (MDCK) cells were cultured in Dulbecco’s modified Eagle medium (DMEM; Gibco) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Microbiology 2022Quote: Madin-Darby canine kidney (MDCK) cells were maintained in minimal essential medium (MEM) (Gibco, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2022Quote: The Spike gene sequence from the severe acute respiratory syndrome coronavirus 2 isolate Wuhan-Hu-1 (NC_045512.2) was codon optimized (GeneArt, ThermoFisher) and gene blocks with overlapping sequences were synthesized by Bio Basic Inc (Markham ...
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bioRxiv - Immunology 2020Quote: ... Anti-SARS-CoV-2 Coronavirus Spike protein (subunit 1) polyclonal antibody was purchased from Invitrogen (Carlsbad, CA, USA). Anti-SARS-CoV-2 Spike RBD rabbit polyclonal antibody was purchased from SinoBiological (Beijing ...
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bioRxiv - Immunology 2022Quote: ... native control peptides and control antigens using a custom-designed antigen microarray (ThermoFisher Scientific, Immunodiagnostics)9 ...
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bioRxiv - Physiology 2022Quote: ... Targeted antigen retrieval was performed by boiling sections in 1x IHC antigen retrieval solution (Invitrogen) for 15-20 mins ...
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bioRxiv - Microbiology 2022Quote: Madin–Darby canine kidney (MDCK) cells were maintained in minimum essential medium (MEM, Thermo Fisher Scientific). African green monkey kidney (Vero ...
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bioRxiv - Microbiology 2020Quote: ... Madin-Darby Canine Kidney (MDCK) cells were grown in Minimal Essential Medium (MEM)(11430-030, Gibco) containing 5% newborn calf serum (16010-159 ...
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bioRxiv - Genetics 2021Quote: ... dataset contained 423 subjects from 37 breeds genotyped for ∼45,000 SNPs (Affymetrix v.2 Canine array)15 ...
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bioRxiv - Cell Biology 2021Quote: ... The cDNAs of canine Tgfα and Nrg1 were synthesized by GeneArt (Thermo Fisher Scientific, Waltham, MA). The cDNA sequences of the cloned growth factors are shown in the supplementary table and note.
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bioRxiv - Microbiology 2022Quote: ... and Madin-Darby canine kidney (MDCK) cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2022Quote: ... Madin Darby canine kidney (MDCK, ATCC) cells were grown in Dulbecco’s modified Eagle’s medium (DMEM, Gibco) supplemented with 10% FBS and penicillin (100 U/ml)-streptomycin (100 μg/ml ...
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bioRxiv - Microbiology 2024Quote: ... For the SARS-CoV-2 staining a polyclonal rabbit anti-SARS Coronavirus Spike protein (PA1-41142, Thermo Fisher, France) and revealed using an anti-rabbit IgG (H+L ...
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bioRxiv - Immunology 2023Quote: ... or control antigen PE (Thermo Fisher) were mixed with adjuvants per manufacturer’s protocols and added to cells immediately after plating ...
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bioRxiv - Immunology 2023Quote: ... Monomeric antigens were biotinylated (ThermoFisher; A39257) at an optimized ratio which resulted in <1 biotin per molecule ...
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bioRxiv - Immunology 2020Quote: ELISA of SARS-CoV-2 antigens was performed by coating antigens on MaxiSorp 96-well plates (ThermoFisher) at 2 µg/mL in 1X PBS [pH 7.4] overnight at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... antigen retrieval techniques were applied human tissue sections using IHC Antigen Retrieval Solution (00-4956-58, Invitrogen). These tissues were stained with Abs against human CD4 pre-labeled with Alexa Fluor 488 using an Ab labeling kit (Molecular Probes ...
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bioRxiv - Microbiology 2022Quote: ... Viruses were grown in Madin-Darby canine kidney (MDCK) cells in Opti-MEM (Life Technologies, Waltham, MA) with 10% fetal calf serum and antibiotics/antimycotics supplemented with 1g/ml tosyl phenylalanyl chloromethyl ketone (TPCK)-trypsin (Worthington Biochemical Corp. ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The MERS-CoV viral lysate was immuno-detected with a MERS-coronavirus spike protein S2 polyclonal antibody (Invitrogen, Carlsbad, CA) followed by a donkey anti-rabbit IgG (H+L) ...
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bioRxiv - Bioengineering 2021Quote: Recombinant coronavirus spike glycoproteins S (Table S1) were coated overnight onto NUNC® Immulon 4 HBX microtiter plates (Thermo Fisher) at 50 ng/well in 100 µL of PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... brain slices underwent an additional antigen retrieval step in 1X IHC Antigen Retrieval Solution (Invitrogen, 00-4956-58) for 15 minutes at 95°C prior to blocking.
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bioRxiv - Cell Biology 2021Quote: ... Viral copy number quantification by RT-qPCR was performed using Detection Kit for 2019 Novel Coronavirus (2019-nCoV) RNA (PCR-Fluorescent Probing) (Cat. #DA-930) (China) in a QuantStudio 3 real-time PCR system (Applied Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2022Quote: ... qRT-PCR analyses were conducted utilizing the Centers for Disease Control 2019-nCoV N1 primers and probe (https://www.cdc.gov/coronavirus/2019-ncov/lab/rt-pcr-panel-primer-probes.html) and AgPath-ID One-Step RT-PCR reagents (Ambion/ThermoFisher, Waltham, Massachusetts). We included a standard curve of serial dilutions of RNA extracted from SARS-CoV-2 virus stock (107 TCID50/ml ...
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bioRxiv - Microbiology 2019Quote: ... and Madin-Darby canine kidney (MDCK) cells (ATCC) were maintained in cell culture media (Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen) supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Microbiology 2020Quote: ... cells (ATCC) and Madin-Darby canine kidney (MDCK) cells (ATCC) were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen) supplemented with 10% FBS ...
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A Polybasic Domain in aPKC Mediates Par6-Dependent Control of Membrane Targeting and Kinase ActivitybioRxiv - Cell Biology 2020Quote: ... Madin-Darby canine kidney (MDCK) II cells were cultured in MEMα media containing 10% fetal bovine serum (Gibco) and 1% penicillin and streptomycin (Gibco) ...
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bioRxiv - Microbiology 2020Quote: ... MDCK (Mardin–Darby canine kidney) and Vero E6 cells (ATCC, no.1586) were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen) with 10 % FBS ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Madin-Darby Canine Kidney (MDCK) cells were transfected with the pcDNA4-Tbx2a construct using Lipofectamine 3000 (ThermoFisher Scientific) following manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: Madin-Darby canine kidney (MDCK; ATCC) and HEK293T (293T) were grown in Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2021Quote: Madin–Darby canine kidney (MDCK) cells were grown in minimal essential medium (MEM) (Thermo Fisher Scientific, MA USA) containing 5% newborn calf serum (16010-159 ...
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bioRxiv - Immunology 2023Quote: ... Stock virus was sub-passaged through Madin-Darby canine kidney (MDCK) cells in Dulbecco’s modified Eagle’s medium (DMEM; Gibco), harvested as tissue culture supernatant and viral titres determined by cytopathic effects on MDCK cells and expressed as the mean log10 tissue culture infective dose required to kill 50% of the cells (TCID50 ...