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Citations for Thermo Fisher :
251 - 300 of 10000+ citations for Canine Adenovirus 2 Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: All RBD antigens were His-tagged and purified using HisPur Ni-NTA resin (Thermo Fisher Scientific, 88222). Cell supernatants were diluted with 1/3 volume of wash buffer (20 mM imidazole ...
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bioRxiv - Cancer Biology 2024Quote: ... Antigen retrieval was performed by the use of Lab Vision PT Module (Thermo Fisher Scientific, Waltham, MA), in 10 mM citrate pH 6 ...
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bioRxiv - Cancer Biology 2024Quote: ... heat-induced (98°C) antigen retrieval was performed with a citrate buffer (pH 6.0) (Thermo Fisher Scientific), and slides were incubated with Triton X-100 0.25% in PBS for 5 minutes ...
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bioRxiv - Immunology 2020Quote: ... that does not express HLA DR and are Class II major histocompatibility (MHC) antigen negative was maintained in Iscove’s Modified Dulbecco’s Medium (IMDM, Invitrogen) supplemented with 20% FBS ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and any antigen–MS-Hu6 complex was captured by goat anti–human HRP–conjugated IgG (Invitrogen, Catalog # A18805).
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bioRxiv - Genetics 2020Quote: ... following de-paraffinization the slides were incubated in eBioscience IHC Antigen Retrieval Solution (ThermoFisher; Cat# 00-4955-58) and washed in 1x PBS three times for five minutes ...
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bioRxiv - Cell Biology 2021Quote: ... The antibody-antigen complex was captured by incubating with lysis buffer prewashed proteinA/G beads (Thermo Fisher Scientific) for 2 h ...
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bioRxiv - Biochemistry 2020Quote: ... The antigen-antibody complexes were detected using the Pierce ECL chemi-luminescent detection system (Thermo Scientific, Rockford, IL) and visualized on the ChemiDoc XRS+ gel documentation system (Bio-Rad).
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bioRxiv - Neuroscience 2020Quote: ... The antigen/antibody complex was eluted from beads by boiling in Lane Marker Sample Buffer (#39001, Thermo Fisher) containing β-mercaptoethanol for SDS-PAGE and Western blotting.
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bioRxiv - Microbiology 2019Quote: ... SV40 T-antigens were detected using (pAb)108 and species-specific AlexaFluor 488 (Life Technologies, Thermo Fisher Scientific) antibodies ...
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bioRxiv - Microbiology 2020Quote: ... using antigens conjugated to NHS-activated agarose beads (Pierce™ NHS-Activated Agarose Spin Columns, Thermo Fisher Scientific). Antibodies were eluted in 0.1M glycine ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit or sheep antigens were raised in donkey and conjugated to Alexa 488 or Alexa 594 (Molecular Probes, ThermoFisher ...
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bioRxiv - Bioengineering 2021Quote: ... and subjected to heat- induced antigen retrieval using sodium citrate buffer(10 mM, pH 6, ThermoFisher, Waltham, MA) at >80°C ...
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bioRxiv - Microbiology 2022Quote: Purified F antigens were biotinylated using an EZ-link Sulfo-NHS -LC-Biotinylation kit (Thermo Fisher, cat#A39257) with a 1:1.3 molar ratio of biotin to F ...
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bioRxiv - Microbiology 2019Quote: ... SV40 T-antigens were detected using (pAb)108 and species-specific AlexaFluor 488 (Life Technologies, Thermo Fisher Scientific) antibodies ...
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bioRxiv - Molecular Biology 2021Quote: ... The antiserum was affinity-purified using the peptide antigen coupled to UltraLink™ Iodoacetyl gel (Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2022Quote: ... after dewaxing and antigen retrieval non-specific antibody binding was minimised by incubating sections in CAS Block (Invitrogen). Primary antibodies were diluted in Dako antibody diluent (S0809 ...
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bioRxiv - Plant Biology 2022Quote: ... Chemiluminescence detection of antigen-antibody complexes was carried out with SuperSignal West Femto Western substrate (Thermo Fisher Scientific). As a loading control ...
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bioRxiv - Neuroscience 2023Quote: ... Antibody/antigen mixture was incubated with Dynabeads M-280 sheep anti-rabbit or mouse IgG (Thermo Fisher Scientific) for 6h at 4°C in the following day ...
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bioRxiv - Neuroscience 2023Quote: ... For antigen retrieval cells were incubated for ten minutes with 1 ug/ml proteinase K (Thermo Fisher Scientific) at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... 200 μL of the virus dilution containing 1µg-3µg p24 antigen was transferred to a poly-D-lysine coated glass coverslip (#1 chambered coverglass, Nunc™ Lab-Tek™ ...
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bioRxiv - Immunology 2023Quote: ... and coronavirus antigens were expressed in Expi293F cells by transient transfection in FreeStyle F17 expression media (Thermo Fisher) supplemented to a final concentration of 0.1% Pluronic Acid F-68 and 20% 4 mM L-glutamine using Expifectamine transfection reagent (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2023Quote: ... the antigens were captured for 15 mins at 4 °C with 50 µl Streptavidin Magnetic beads (Thermo Fisher) which were previously incubated with superblock (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... Target antigens were finally detected using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, Waltham, MA). Images were scanned and analyzed using ImageJ software ...
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bioRxiv - Neuroscience 2020Quote: ... The slides were placed in 250 ml freshly made antigen retrieval solution (0.1 M sodium citrate buffer pH 6.0, Fisher Scientific) and pressure cooked (A ...
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bioRxiv - Immunology 2022Quote: ... The antigen binding fragment (Fab) portion of LY-CoV1404 was generated by proteolytic digestion using immobilized papain (ThermoFisher Scientific), followed by removal of un-cleaved protein using standard chromatography techniques ...
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bioRxiv - Systems Biology 2019Quote: Recombinant protein antigens were first labeled with biotin using EZ-Link™ NHS-LC-Biotin reagent (Thermo Fisher, #21366) and then hybridized with the PETAL array ...
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bioRxiv - Neuroscience 2021Quote: ... Immunoreactivity to the antigen was visualized using Alexa Fluor 488-or Alexa Fluor 594-conjugated secondary antibody (Molecular Probes). Nuclear staining was performed with Hoechst 33258 (Polysciences) ...
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bioRxiv - Microbiology 2020Quote: ... the cells were washed in PBS and incubated for 1-h at RT with 1 mL anti-Early Endosomal Antigen 1 (EEA1) monoclonal antibody (F. 43.1; Invitrogen) diluted 1:200 in blocking buffer ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit or sheep antigens were raised in donkey and conjugated to Alexa 488 or Alexa 594 (Molecular Probes, ThermoFisher, RRIDs ...
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bioRxiv - Immunology 2020Quote: ... Anti-mouse monoclonal antibodies targeting cell surface antigens included anti-GL7 (FITC, PE, eFluor660; clone GL 7; Thermo Fisher), anti-B220 (PE-Cy7 ...
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bioRxiv - Immunology 2022Quote: Vaccine 5: The above mentioned hCMP-mRBD antigen when expressed from Stable CHO (Thermo Fisher Scientific, Cat no. R75807) cell lines was named as hCMP-mRBD-CHO ...
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bioRxiv - Cancer Biology 2020Quote: ... The beads with antigens captured were loaded onto a SDS-PAGE gel (4-20% gradient polyacrylamide) (Thermo Fisher Scientific) in duplicates ...
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bioRxiv - Immunology 2020Quote: ... Antigen-specific IgG was purified from pooled serum with UF6b-conjugated Dynabeads™ M-270 Epoxy (Thermo Fisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... The antigen peptide with N-terminus Cysteine (NH2-CGGGGAAPGPPRHG-COOH) was coupled with keyhole limpet hemocyanin (Thermo fisher 77600). The antigen was injected into rabbit and antisera were collected after primary and several booster injections ...
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bioRxiv - Cancer Biology 2021Quote: ... The antigen-antibody complexes on the membrane were detected using labeled secondary antibodies and enhanced chemiluminescence reagents (Thermo Scientific). The antibodies and their dilutions were as follows ...
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bioRxiv - Immunology 2020Quote: ... total IgG against these antigens were detected using HRP-conjugated respective anti-mouse antibody (Sigma-Aldrich, eBioscience and Invitrogen) and the reaction was detected using TMB substrate (BD biosciences) ...
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bioRxiv - Biochemistry 2022Quote: ... and LacI antibodies were affinity-purified from serum using the appropriate antigen coupled to AminoLink Coupling Resin (Thermo Scientific) according to manufacturer’s protocol.
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bioRxiv - Developmental Biology 2022Quote: ... were used to detect antigens and DNA was stained with DAPI in the mounting medium (ThermoFisher Scientific, Cat# P36941).
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bioRxiv - Physiology 2022Quote: ... Non-specific antigens were blocked for 60 min using a blocking buffer (Sea Block Blocking Buffer; ThermoFisher Scientific, USA). Then ...
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bioRxiv - Microbiology 2022Quote: All antigens and FcγRs were equilibrated in 1X PBS using Zeba-Spin desalting and size exclusion chromatography columns (ThermoFisher) prior to bead coupling ...
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bioRxiv - Cell Biology 2023Quote: ... The antigen-antibody complex was detected by SuperSignal™ West Dura Extended Duration Chemiluminescent Substrate (Cat#34075, Thermo Fisher).
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bioRxiv - Immunology 2021Quote: ... for 2-18 hours at 4°C and stained with cocktails of fluorescently-labeled antibodies against intracellular antigens diluted in Permeabilization Buffer (Invitrogen) for 20 minutes at room temperature followed by 20 minutes at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... anti-mouse surface antigen 1 (SAG1) mab DG52 1:500 46 and then with goat anti-rat-Alexa546 1:300 (Invitrogen), goat anti-rat-Alexa375 1:300 (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: All antigens were biotinylated using an NHS-Sulfo-LC-LC kit according to the manufacturer’s instruction (Thermo Fisher, MA, USA) if required by the assay and excessive biotin was removed by size exclusion chromatography using Zeba-Spin desalting columns (7kDa cutoff ...
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bioRxiv - Immunology 2021Quote: ... cells were fixed and permeabilized using the Foxp3/Transcription Factor Staining Buffer Set overnight and stained with antibodies against intracellular antigens (1 µg/ml) in Permeabilization buffer (ThermoFisher). For biotinylated antibodies ...
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bioRxiv - Immunology 2021Quote: ... Induced yeast libraries were stained for antigen binding (RBD-Fc APC tetramers) and scFv expression (chicken anti-c-myc IgY; Invitrogen). Following two washes in PBSF (1x PBS ...
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bioRxiv - Bioengineering 2019Quote: Paraffin-embedded aorta sections mounted slides were subjected to heat-induced antigen epitope retrieval with citrate buffer (Thermo Scientific, MA). The slides were incubated overnight at 4°C with primary antibodies for anti -MMP-9 (Rabbit anti-Mouse ...
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bioRxiv - Microbiology 2021Quote: ... fixed parasites were incubated with 5µg/mL Blood Group Antigen H (O) Type 1 monoclonal antibody (Invitrogen 13-9810-82), washed three times with 1x PBS and incubated with 1:100 α-mouse secondary antibody conjugated to Alexa 488 (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... Phage display selections were carried out by alternating between capture of the antigen on neutravidin coated wells on Maxisorp plates (Nunc) and streptavidin magnetic beads (Invitrogen ...